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| Title | Cryo-EM with sub-1 Å specimen movement. |
|---|---|
| Journal, issue, pages | Science, Vol. 370, Issue 6513, Page 223-226, Year 2020 |
| Publish date | Oct 9, 2020 |
 Authors | Katerina Naydenova / Peipei Jia / Christopher J Russo /   |
| PubMed Abstract | Most information loss in cryogenic electron microscopy (cryo-EM) stems from particle movement during imaging, which remains poorly understood. We show that this movement is caused by buckling and ...Most information loss in cryogenic electron microscopy (cryo-EM) stems from particle movement during imaging, which remains poorly understood. We show that this movement is caused by buckling and subsequent deformation of the suspended ice, with a threshold that depends directly on the shape of the frozen water layer set by the support foil. We describe a specimen support design that eliminates buckling and reduces electron beam-induced particle movement to less than 1 angstrom. The design allows precise foil tracking during imaging with high-speed detectors, thereby lessening demands on cryostage precision and stability. It includes a maximal density of holes, which increases throughput in automated cryo-EM without degrading data quality. Movement-free imaging allows extrapolation to a three-dimensional map of the specimen at zero electron exposure, before the onset of radiation damage. |
 External links | Science / PubMed:33033219 / PubMed Central |
| Methods | EM (single particle) |
| Resolution | 1.9 Å |
| Structure data | EMDB-11210, PDB-6zgl: |
| Chemicals |  ChemComp-HOH: |
| Source |
|
 Keywords | DNA BINDING PROTEIN / DNA-BINDING PROTEIN |
escherichia coli (E. coli)
