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| Title | Structure of a prehandover mammalian ribosomal SRP·SRP receptor targeting complex. |
|---|---|
| Journal, issue, pages | Science, Vol. 360, Issue 6386, Page 323-327, Year 2018 |
| Publish date | Apr 20, 2018 |
 Authors | Kan Kobayashi / Ahmad Jomaa / Jae Ho Lee / Sowmya Chandrasekar / Daniel Boehringer / Shu-Ou Shan / Nenad Ban /   |
| PubMed Abstract | Signal recognition particle (SRP) targets proteins to the endoplasmic reticulum (ER). SRP recognizes the ribosome synthesizing a signal sequence and delivers it to the SRP receptor (SR) on the ER ...Signal recognition particle (SRP) targets proteins to the endoplasmic reticulum (ER). SRP recognizes the ribosome synthesizing a signal sequence and delivers it to the SRP receptor (SR) on the ER membrane followed by the transfer of the signal sequence to the translocon. Here, we present the cryo-electron microscopy structure of the mammalian translating ribosome in complex with SRP and SR in a conformation preceding signal sequence handover. The structure visualizes all eukaryotic-specific SRP and SR proteins and reveals their roles in stabilizing this conformation by forming a large protein assembly at the distal site of SRP RNA. We provide biochemical evidence that the guanosine triphosphate hydrolysis of SRP·SR is delayed at this stage, possibly to provide a time window for signal sequence handover to the translocon. |
 External links | Science / PubMed:29567807 / PubMed Central |
| Methods | EM (single particle) |
| Resolution | 3.7 Å |
| Structure data | |
| Chemicals |  ChemComp-MG:  ChemComp-ZN:  ChemComp-GTP:  ChemComp-GNP: |
| Source |
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 Keywords | TRANSLATION / ER membrane targeting ribosome Signal recognition particle |
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