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Structure paper

Title"Mix-it-up": Accessible time-resolved cryo-EM on the millisecond timescale
Journal, issue, pagesTo Be Published
Publish dateMay 20, 2025 (structure data deposition date)
AuthorsAlexandrescu L / Lander GC
External linksSearch PubMed
MethodsEM (single particle)
Resolution2.9 - 7.1 Å
Structure data

EMDB-70747: Mouse heavy-chain apoferritin from mixed sample prepared with Mix-it-up
Method: EM (single particle) / Resolution: 2.9 Å

EMDB-70748: Rabbit muscle aldolase from mixed sample prepared with Mix-it-up
Method: EM (single particle) / Resolution: 2.9 Å

EMDB-70749: Cowpea chlorotic mottle virus in the contracted state
Method: EM (single particle) / Resolution: 3.2 Å

EMDB-70750: Cowpea chlorotic mottle virus in the expanded state
Method: EM (single particle) / Resolution: 6.9 Å

EMDB-70751: Cowpea chlorotic mottle virus in contracted state 700 ms after pH shift triggered using Mix-it-up
Method: EM (single particle) / Resolution: 3.9 Å

EMDB-70752: E.coli GroEL in presence of GroES and in absence of ATP
Method: EM (single particle) / Resolution: 3.2 Å

EMDB-70753: E.coli GroEL in presence of GroES 300ms after addition of ATP
Method: EM (single particle) / Resolution: 3.6 Å

EMDB-70754: E.coli GroEL/ES in the bullet conformation 300ms after addition of ATP
Method: EM (single particle) / Resolution: 3.3 Å

EMDB-70755: E.coli GroEL/ES in the football conformation 300ms after addition of ATP
Method: EM (single particle) / Resolution: 4.8 Å

EMDB-70756: E.coli GroEL in presence of GroES 700ms after addition of ATP
Method: EM (single particle) / Resolution: 4.65 Å

EMDB-70757: E.coli GroEL/ES in the bullet conformation 700ms after addition of ATP
Method: EM (single particle) / Resolution: 3.5 Å

EMDB-70758: E.coli GroEL/ES in the football conformation 700ms after addition of ATP
Method: EM (single particle) / Resolution: 7.1 Å

EMDB-70759: E.coli GroEL in presence of GroES 100ms after addition of ATP
Method: EM (single particle) / Resolution: 3.4 Å

Source
  • Mus musculus (house mouse)
  • Oryctolagus cuniculus (rabbit)
  • Cowpea chlorotic mottle virus
  • Escherichia coli (E. coli)

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