National Institutes of Health/National Center for Research Resources (NIH/NCRR)
R01GM102498
United States
National Institutes of Health/National Center for Research Resources (NIH/NCRR)
R01GM098672
United States
National Institutes of Health/National Center for Research Resources (NIH/NCRR)
S10OD020054
United States
National Institutes of Health/National Center for Research Resources (NIH/NCRR)
S10OD021741
United States
National Institutes of Health/National Center for Research Resources (NIH/NCRR)
R35GM122530
United States
Citation
Journal: Cell / Year: 2018 Title: Structural Basis for Cholesterol Transport-like Activity of the Hedgehog Receptor Patched. Authors: Yunxiao Zhang / David P Bulkley / Yao Xin / Kelsey J Roberts / Daniel E Asarnow / Ashutosh Sharma / Benjamin R Myers / Wonhwa Cho / Yifan Cheng / Philip A Beachy / Abstract: Hedgehog protein signals mediate tissue patterning and maintenance by binding to and inactivating their common receptor Patched, a 12-transmembrane protein that otherwise would suppress the activity ...Hedgehog protein signals mediate tissue patterning and maintenance by binding to and inactivating their common receptor Patched, a 12-transmembrane protein that otherwise would suppress the activity of the 7-transmembrane protein Smoothened. Loss of Patched function, the most common cause of basal cell carcinoma, permits unregulated activation of Smoothened and of the Hedgehog pathway. A cryo-EM structure of the Patched protein reveals striking transmembrane domain similarities to prokaryotic RND transporters. A central hydrophobic conduit with cholesterol-like contents courses through the extracellular domain and resembles that used by other RND proteins to transport substrates, suggesting Patched activity in cholesterol transport. Cholesterol activity in the inner leaflet of the plasma membrane is reduced by PTCH1 expression but rapidly restored by Hedgehog stimulation, suggesting that PTCH1 regulates Smoothened by controlling cholesterol availability.
History
Deposition
Sep 13, 2018
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Header (metadata) release
Nov 28, 2018
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Map release
Nov 28, 2018
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Update
Mar 16, 2022
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Current status
Mar 16, 2022
Processing site: RCSB / Status: Released
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Structure visualization
Movie
Surface view with section colored by density value
Model: Quantifoil R1.2/1.3 / Material: COPPER / Mesh: 400 / Support film - Material: CARBON / Support film - topology: HOLEY / Pretreatment - Type: GLOW DISCHARGE / Pretreatment - Atmosphere: AIR
Vitrification
Cryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 23 K / Instrument: FEI VITROBOT MARK III
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Electron microscopy
Microscope
FEI TITAN KRIOS
Image recording
Film or detector model: GATAN K2 SUMMIT (4k x 4k) / Detector mode: SUPER-RESOLUTION / Number grids imaged: 1 / Number real images: 5236 / Average exposure time: 8.0 sec. / Average electron dose: 38.0 e/Å2
Electron beam
Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron optics
Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Cs: 2.7 mm
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company
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Image processing
Particle selection
Number selected: 378828 Details: Number of particles selected after rough initial 2D classification
CTF correction
Software - Name: Gctf
Startup model
Type of model: NONE / Details: Ab initio model generated from CryoSparc
Final reconstruction
Applied symmetry - Point group: C1 (asymmetric) / Resolution.type: BY AUTHOR / Resolution: 3.6 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: RELION (ver. 2) / Number images used: 245725
Initial angle assignment
Type: MAXIMUM LIKELIHOOD / Software - Name: cryoSPARC (ver. 1) Details: Initial angles were assigned by ab initio reconstruction in CryoSparc
Final angle assignment
Type: MAXIMUM LIKELIHOOD / Software - Name: RELION (ver. 2)
Final 3D classification
Software - Name: RELION (ver. 2)
FSC plot (resolution estimation)
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Atomic model buiding 1
Refinement
Space: REAL / Protocol: AB INITIO MODEL
Output model
PDB-6mg8: Structural basis for cholesterol transport-like activity of the Hedgehog receptor Patched
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