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- EMDB-47628: Cryo-EM structure of the Pyrobaculum calidifontis 70S ribosome (C... -

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Open data

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Basic information

Entry

Database: EMDB / ID: EMD-47628

Title

Cryo-EM structure of the Pyrobaculum calidifontis 70S ribosome (Composite Map)

Map data

Pyrobaculum calidifontis 70S ribosome (composite map)

Sample

Complex: 70S ribosome
- Complex: 50S Subunit
  - RNA: x 2 types
  - Protein or peptide: x 36 types
- Complex: 30S subunit
  - RNA: x 1 types
  - Protein or peptide: x 29 types
Ligand: x 4 types

Keywords

Ribosome / Translation / Hibernation / RNA

Function / homology

Function and homology information

ribonuclease P activity / tRNA 5'-leader removal / cytosolic ribosome / ribosomal large subunit biogenesis / large ribosomal subunit / ribosome biogenesis / ribosomal small subunit assembly / large ribosomal subunit rRNA binding / 5S rRNA binding / small ribosomal subunit ...
Similarity search - Function

Small zinc finger protein HVO_2753-like, zinc-binding pocket / Small zinc finger protein HVO_2753-like / Small zinc finger protein HVO_2753-like, Zn-binding pocket / Ribosomal protein L14e / : / Ribosomal protein L40e, archaeal / Ribosomal protein S9, archaeal / Ribosomal protein S13, archaeal / Ribosomal protein S17, archaeal / Ribosomal protein S6e, archaeal ...Small zinc finger protein HVO_2753-like, zinc-binding pocket / Small zinc finger protein HVO_2753-like / Small zinc finger protein HVO_2753-like, Zn-binding pocket / Ribosomal protein L14e / : / Ribosomal protein L40e, archaeal / Ribosomal protein S9, archaeal / Ribosomal protein S13, archaeal / Ribosomal protein S17, archaeal / Ribosomal protein S6e, archaeal / Ribosomal protein S4, archaeal / Ribosomal protein S12, archaea / Ribosomal protein S3, archaeal / 30S ribosomal protein S3Ae / : / Ribosomal protein S7, archaeal / Ribosomal protein S19e, archaeal / Ribosomal protein S11, archaeal / Ribosomal protein S2, archaeal / Ribosomal protein S14, type Z, archaeal / Ribosomal protein S8e, archaeal / Ribosomal protein L19e, archaeal / Ribosomal protein L30, archaeal / Ribosomal protein L6P, archaea / Ribosomal protein L14P, archaeal / Ribosomal protein L21e, archaeal / Ribosomal protein L18e, archaea / Ribosomal protein L10e, archaea / Ribosomal protein L32e, archaeal / Ribosomal protein L3, archaeal / Ribosomal protein L4, archaea / Ribosomal protein L5, archaeal / Ribosomal protein L7Ae, archaea / DJ-1/PfpI / DJ-1/PfpI family / Ribosomal protein L24e / Ribosomal protein L30e / Ribosomal protein L2, archaeal-type / Ribosomal L15/L27a, N-terminal / Ribosomal protein L23 / : / Class I glutamine amidotransferase-like / metallochaperone-like domain / TRASH domain / Ribosomal protein S26e / Ribosomal protein S26e superfamily / Ribosomal protein S26e / Ribosomal protein S5, eukaryotic/archaeal / Ribosomal protein S19e, conserved site / Ribosomal protein S19e signature. / Ribosomal protein L13e, conserved site / Ribosomal protein L13e signature. / Ribosomal protein L38e / Ribosomal protein L38e superfamily / Ribosomal L38e protein family / Ribosomal protein S10, eukaryotic/archaeal / Ribosomal protein L10e, conserved site / Ribosomal protein L10e signature. / Ribosomal protein L10e / Ribosomal protein S25 / S25 ribosomal protein / Ribosomal protein L13e / Ribosomal protein L13e / Ribosomal protein S2, eukaryotic/archaeal / Ribosomal protein S17e, conserved site / Ribosomal protein S17e signature. / Ribosomal protein S30 / Ribosomal protein S30 / Ribosomal protein L24e, conserved site / 40S ribosomal protein S29/30S ribosomal protein S14 type Z / Ribosomal protein L24e signature. / Ribosomal protein L34e, conserved site / Ribosomal protein L34e signature. / Ribosomal protein S3, eukaryotic/archaeal / 50S ribosomal protein L18Ae/60S ribosomal protein L20 and L18a / Ribosomal protein S19e / Ribosomal protein S19e / Ribosomal_S19e / Ribosomal protein 50S-L18Ae/60S-L20/60S-L18A / Ribosomal L40e family / Ribosomal proteins 50S-L18Ae/60S-L20/60S-L18A / Ribosomal protein S27e signature. / Ribosomal_L40e / Ribosomal protein L40e / Ribosomal protein L40e superfamily / Ribosomal protein 60S L18 and 50S L18e / Ribosomal protein L30e signature 2. / Ribosomal protein L30e, conserved site / Ribosomal protein S19A/S15e / Ribosomal protein L39e, conserved site / Ribosomal protein L39e signature. / Ribosomal protein S17e / Ribosomal protein S17e-like superfamily / Ribosomal S17 / Ribosomal protein L34Ae / Ribosomal protein L34e / : / 60S ribosomal protein L19 / Ribosomal protein L30/YlxQ / Ribosomal protein S4e, N-terminal
Similarity search - Domain/homology

Small ribosomal subunit protein uS7 / Large ribosomal subunit protein uL2 / Small ribosomal subunit protein eS17 / Small ribosomal subunit protein uS2 / Large ribosomal subunit protein eL14 / Large ribosomal subunit protein uL18 / Large ribosomal subunit protein eL19 / Large ribosomal subunit protein eL32 / Small ribosomal subunit protein uS8 / Small ribosomal subunit protein uS14 ...Small ribosomal subunit protein uS7 / Large ribosomal subunit protein uL2 / Small ribosomal subunit protein eS17 / Small ribosomal subunit protein uS2 / Large ribosomal subunit protein eL14 / Large ribosomal subunit protein uL18 / Large ribosomal subunit protein eL19 / Large ribosomal subunit protein eL32 / Small ribosomal subunit protein uS8 / Small ribosomal subunit protein uS14 / SSU ribosomal protein S30E / Small ribosomal subunit protein eS24 / Small ribosomal subunit protein uS10 / Large ribosomal subunit protein eL24 / Large ribosomal subunit protein eL8 / Large ribosomal subunit protein uL29 / Small ribosomal subunit protein uS3 / Large ribosomal subunit protein uL22 / DJ-1/PfpI domain-containing protein / Large ribosomal subunit protein eL13 / LSU ribosomal protein L38E / Small ribosomal subunit protein uS19 / Small ribosomal subunit protein uS17 / Large ribosomal subunit protein uL30 / Small ribosomal subunit protein uS5 / Small ribosomal subunit protein eS6 / Large ribosomal subunit protein eL43 / Small ribosomal subunit protein uS4 / Small ribosomal subunit protein uS13 / Large ribosomal subunit protein eL39 / Small ribosomal subunit protein eS19 / Small ribosomal subunit protein eS27 / Large ribosomal subunit protein uL15 / SSU ribosomal protein S25E / SSU ribosomal protein S26E / Large ribosomal subunit protein eL34 / Small ribosomal subunit protein uS15 / Small ribosomal subunit protein eS1 / Large ribosomal subunit protein uL3 / Large ribosomal subunit protein uL4 / Large ribosomal subunit protein uL23 / 50S ribosomal protein L15e / PaREP1 domain containing protein / Large ribosomal subunit protein uL6 / Large ribosomal subunit protein eL21 / Small ribosomal subunit protein eS8 / Small ribosomal subunit protein eS4 / Large ribosomal subunit protein uL24 / Large ribosomal subunit protein uL14 / Small ribosomal subunit protein uS11 / Large ribosomal subunit protein eL31 / Large ribosomal subunit protein eL20 / Large ribosomal subunit protein eL40 / Large ribosomal subunit protein uL16 / Large ribosomal subunit protein uL5 / Large ribosomal subunit protein eL30 / Small ribosomal subunit protein uS12 / Large ribosomal subunit protein eL18 / Large ribosomal subunit protein uL13 / Small ribosomal subunit protein uS9 / Small zinc finger protein HVO-2753-like zinc-binding pocket domain-containing protein / Large ribosomal subunit protein eL37
Similarity search - Component

Biological species

Pyrobaculum calidifontis JCM 11548 (archaea)

Method

single particle reconstruction / cryo EM / Resolution: 2.36 Å

Authors

Nissley AJ / Cate JHD

Funding support

United States, 1 items

Citation

Journal: To Be Published
Title: Structure of an Archaeal Ribosome with a Divergent Active Site
Authors: Nissley AJ / Shulgina Y / Kivimae RW / Downing BE / Penev PI / Banfield JF / Nayak DD / Cate JHD

History

Deposition	Oct 31, 2024	-
Header (metadata) release	Nov 20, 2024	-
Map release	Nov 20, 2024	-
Update	Nov 20, 2024	-
Current status	Nov 20, 2024	Processing site: RCSB / Status: Released

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Structure visualization

Supplemental images	emd_47628.png

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Downloads & links

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EMDB archive

Map data	emd_47628.map.gz	744.1 MB		EMDB map data format
Header (meta data)	emd-47628-v30.xml emd-47628.xml	95.7 KB 95.7 KB	Display Display	EMDB header
Images	emd_47628.png	154.6 KB
Filedesc metadata	emd-47628.cif.gz	16.7 KB
Archive directory	http://ftp.pdbj.org/pub/emdb/structures/EMD-47628 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-47628	HTTPS FTP

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Validation report

Summary document	emd_47628_validation.pdf.gz	618.2 KB	Display	EMDB validaton report
Full document	emd_47628_full_validation.pdf.gz	617.7 KB	Display
Data in XML	emd_47628_validation.xml.gz	8.5 KB	Display
Data in CIF	emd_47628_validation.cif.gz	9.9 KB	Display
Arichive directory	https://ftp.pdbj.org/pub/emdb/validation_reports/EMD-47628 ftp://ftp.pdbj.org/pub/emdb/validation_reports/EMD-47628	HTTPS FTP

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Related structure data

Related structure data	9e71MC 47578C 47604C 47605C 47606C 47611C 47617C 47662C 47664C 47666C 47667C 47668C 9e6qC 9e7fC C: citing same article (ref.) M: atomic model generated by this map
Similar structure data	Similarity search - Function & homologyF&H Search Similarity search - FunctionF&H Search Similarity search - HomologyF&H Search

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Links

EMDB pages	EMDB (EBI/PDBe) / EMDataResource
Related items in Molecule of the Month	#121 - Jan 2010 70S Ribosomes similarity (83) #10 - Oct 2000 Ribosome similarity (82) #161 - May 2013 Ricin similarity (141) #266 - Feb 2022 Oligosaccharyltransferase similarity (63) #60 - Dec 2004 Ubiquitin similarity (4) #260 - Aug 2021 Ribonuclease P similarity (6) #215 - Nov 2017 Aspartate Transcarbamoylase similarity (1) #57 - Sep 2004 Catalase similarity (1)

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Map

File

Download / File: emd_47628.map.gz / Format: CCP4 / Size: 857.4 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)

Annotation

Pyrobaculum calidifontis 70S ribosome (composite map)

Projections & slices

Image control

Size
Brightness
Contrast
Others	InvertY flip

Axes	Z (Sec.)	Y (Row.)	X (Col.)
	0.83 Å/pix. x 608 pix. = 504.214 Å	0.83 Å/pix. x 608 pix. = 504.214 Å	0.83 Å/pix. x 608 pix. = 504.214 Å
Surface
Projections
Slices (1/3)
Slices (1/2)
Slices (2/3)

Images are generated by Spider.

Voxel size

X=Y=Z: 0.8293 Å

Density

Histogram

Histogram (log scale)

Contour Level	By AUTHOR: 0.215
Minimum - Maximum	-0.61848336 - 1.5264345
Average (Standard dev.)	-0.000000000048487 (±0.049015474)

Symmetry

Space group: 1

Details

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Supplemental data

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Sample components

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Entire : 70S ribosome

Entire	Name: 70S ribosome
Components	Complex: 70S ribosome Complex: 50S Subunit RNA: 5S rRNA RNA: 23S rRNA Protein or peptide: Large ribosomal subunit protein uL2 Protein or peptide: Large ribosomal subunit protein uL3 Protein or peptide: Large ribosomal subunit protein uL4 Protein or peptide: Large ribosomal subunit protein uL5 Protein or peptide: Large ribosomal subunit protein uL6 Protein or peptide: Large ribosomal subunit protein eL8 Protein or peptide: Large ribosomal subunit protein uL13 Protein or peptide: Large ribosomal subunit protein eL13 Protein or peptide: Large ribosomal subunit protein uL14 Protein or peptide: Large ribosomal subunit protein eL14 Protein or peptide: Large ribosomal subunit protein uL15 Protein or peptide: 50S ribosomal protein L15e Protein or peptide: Large ribosomal subunit protein uL16 Protein or peptide: Large ribosomal subunit protein uL18 Protein or peptide: Large ribosomal subunit protein eL18 Protein or peptide: Large ribosomal subunit protein eL19 Protein or peptide: Large ribosomal subunit protein eL20 Protein or peptide: Large ribosomal subunit protein eL21 Protein or peptide: Large ribosomal subunit protein uL22 Protein or peptide: Large ribosomal subunit protein uL23 Protein or peptide: Large ribosomal subunit protein uL24 Protein or peptide: Large ribosomal subunit protein eL24 Protein or peptide: Large ribosomal subunit protein uL29 Protein or peptide: Large ribosomal subunit protein uL30 Protein or peptide: Large ribosomal subunit protein eL30 Protein or peptide: Large ribosomal subunit protein eL31 Protein or peptide: Large ribosomal subunit protein eL32 Protein or peptide: Large ribosomal subunit protein eL34 Protein or peptide: Large ribosomal subunit protein eL37 Protein or peptide: LSU ribosomal protein L38E Protein or peptide: Large ribosomal subunit protein eL39 Protein or peptide: Large ribosomal subunit protein eL40 Protein or peptide: eL42 Protein or peptide: Large ribosomal subunit protein eL43 Protein or peptide: DJ-1/PfpI domain-containing protein Protein or peptide: PaREP1 domain containing protein Complex: 30S subunit RNA: 16S rRNA Protein or peptide: Small ribosomal subunit protein eS1 Protein or peptide: Small ribosomal subunit protein uS2 Protein or peptide: Small ribosomal subunit protein uS3 Protein or peptide: Small ribosomal subunit protein uS4 Protein or peptide: Small ribosomal subunit protein eS4 Protein or peptide: Small ribosomal subunit protein uS5 Protein or peptide: Small ribosomal subunit protein eS6 Protein or peptide: Small ribosomal subunit protein uS7 Protein or peptide: Small ribosomal subunit protein uS8 Protein or peptide: Small ribosomal subunit protein eS8 Protein or peptide: Small ribosomal subunit protein uS9 Protein or peptide: Small ribosomal subunit protein uS10 Protein or peptide: Small ribosomal subunit protein uS11 Protein or peptide: Small ribosomal subunit protein uS12 Protein or peptide: Small ribosomal subunit protein uS13 Protein or peptide: Small ribosomal subunit protein uS14 Protein or peptide: Small ribosomal subunit protein uS15 Protein or peptide: Small ribosomal subunit protein uS17 Protein or peptide: Small ribosomal subunit protein eS17 Protein or peptide: Small ribosomal subunit protein uS19 Protein or peptide: Small ribosomal subunit protein eS19 Protein or peptide: Small ribosomal subunit protein eS24 Protein or peptide: SSU ribosomal protein S25E Protein or peptide: SSU ribosomal protein S26E Protein or peptide: Small ribosomal subunit protein eS27 Protein or peptide: eS28 Protein or peptide: SSU ribosomal protein S30E Protein or peptide: aS35 Protein or peptide: Small zinc finger protein HVO-2753-like zinc-binding pocket domain-containing protein Ligand: SPERMINE Ligand: MAGNESIUM ION Ligand: ZINC ION Ligand: water

Entire

Name: 70S ribosome

Components

Complex: 70S ribosome
- Complex: 50S Subunit
  - RNA: 5S rRNA
  - RNA: 23S rRNA
  - Protein or peptide: Large ribosomal subunit protein uL2
  - Protein or peptide: Large ribosomal subunit protein uL3
  - Protein or peptide: Large ribosomal subunit protein uL4
  - Protein or peptide: Large ribosomal subunit protein uL5
  - Protein or peptide: Large ribosomal subunit protein uL6
  - Protein or peptide: Large ribosomal subunit protein eL8
  - Protein or peptide: Large ribosomal subunit protein uL13
  - Protein or peptide: Large ribosomal subunit protein eL13
  - Protein or peptide: Large ribosomal subunit protein uL14
  - Protein or peptide: Large ribosomal subunit protein eL14
  - Protein or peptide: Large ribosomal subunit protein uL15
  - Protein or peptide: 50S ribosomal protein L15e
  - Protein or peptide: Large ribosomal subunit protein uL16
  - Protein or peptide: Large ribosomal subunit protein uL18
  - Protein or peptide: Large ribosomal subunit protein eL18
  - Protein or peptide: Large ribosomal subunit protein eL19
  - Protein or peptide: Large ribosomal subunit protein eL20
  - Protein or peptide: Large ribosomal subunit protein eL21
  - Protein or peptide: Large ribosomal subunit protein uL22
  - Protein or peptide: Large ribosomal subunit protein uL23
  - Protein or peptide: Large ribosomal subunit protein uL24
  - Protein or peptide: Large ribosomal subunit protein eL24
  - Protein or peptide: Large ribosomal subunit protein uL29
  - Protein or peptide: Large ribosomal subunit protein uL30
  - Protein or peptide: Large ribosomal subunit protein eL30
  - Protein or peptide: Large ribosomal subunit protein eL31
  - Protein or peptide: Large ribosomal subunit protein eL32
  - Protein or peptide: Large ribosomal subunit protein eL34
  - Protein or peptide: Large ribosomal subunit protein eL37
  - Protein or peptide: LSU ribosomal protein L38E
  - Protein or peptide: Large ribosomal subunit protein eL39
  - Protein or peptide: Large ribosomal subunit protein eL40
  - Protein or peptide: eL42
  - Protein or peptide: Large ribosomal subunit protein eL43
  - Protein or peptide: DJ-1/PfpI domain-containing protein
  - Protein or peptide: PaREP1 domain containing protein
- Complex: 30S subunit
  - RNA: 16S rRNA
  - Protein or peptide: Small ribosomal subunit protein eS1
  - Protein or peptide: Small ribosomal subunit protein uS2
  - Protein or peptide: Small ribosomal subunit protein uS3
  - Protein or peptide: Small ribosomal subunit protein uS4
  - Protein or peptide: Small ribosomal subunit protein eS4
  - Protein or peptide: Small ribosomal subunit protein uS5
  - Protein or peptide: Small ribosomal subunit protein eS6
  - Protein or peptide: Small ribosomal subunit protein uS7
  - Protein or peptide: Small ribosomal subunit protein uS8
  - Protein or peptide: Small ribosomal subunit protein eS8
  - Protein or peptide: Small ribosomal subunit protein uS9
  - Protein or peptide: Small ribosomal subunit protein uS10
  - Protein or peptide: Small ribosomal subunit protein uS11
  - Protein or peptide: Small ribosomal subunit protein uS12
  - Protein or peptide: Small ribosomal subunit protein uS13
  - Protein or peptide: Small ribosomal subunit protein uS14
  - Protein or peptide: Small ribosomal subunit protein uS15
  - Protein or peptide: Small ribosomal subunit protein uS17
  - Protein or peptide: Small ribosomal subunit protein eS17
  - Protein or peptide: Small ribosomal subunit protein uS19
  - Protein or peptide: Small ribosomal subunit protein eS19
  - Protein or peptide: Small ribosomal subunit protein eS24
  - Protein or peptide: SSU ribosomal protein S25E
  - Protein or peptide: SSU ribosomal protein S26E
  - Protein or peptide: Small ribosomal subunit protein eS27
  - Protein or peptide: eS28
  - Protein or peptide: SSU ribosomal protein S30E
  - Protein or peptide: aS35
  - Protein or peptide: Small zinc finger protein HVO-2753-like zinc-binding pocket domain-containing protein
Ligand: SPERMINE
Ligand: MAGNESIUM ION
Ligand: ZINC ION
Ligand: water

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Supramolecule #1: 70S ribosome

Supramolecule	Name: 70S ribosome / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#68
Source (natural)	Organism: Pyrobaculum calidifontis JCM 11548 (archaea)

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Supramolecule #2: 50S Subunit

Supramolecule	Name: 50S Subunit / type: complex / ID: 2 / Parent: 1 / Macromolecule list: #1-#2, #4-#39
Source (natural)	Organism: Pyrobaculum calidifontis JCM 11548 (archaea)

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Supramolecule #3: 30S subunit

Supramolecule	Name: 30S subunit / type: complex / ID: 3 / Parent: 1 / Macromolecule list: #3, #40-#68
Source (natural)	Organism: Pyrobaculum calidifontis JCM 11548 (archaea)

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Macromolecule #1: 5S rRNA

Macromolecule	Name: 5S rRNA / type: rna / ID: 1 / Number of copies: 1
Source (natural)	Organism: Pyrobaculum calidifontis JCM 11548 (archaea)
Molecular weight	Theoretical: 41.803953 KDa
Sequence	String: GCCCCUAACC CGGCCAUAGG CGCCGGUGCU ACACCCGGUC UCAUCAGAAC CCGGAAGUUA AGGCCGGCGC CGCGCUCGGG AGUACUGGG CUCCGCGAGG GCCCGGGAAA CCGGCGUGCU GGGAGGGGGC GENBANK: GENBANK: CP000561.1

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Macromolecule #2: 23S rRNA

Macromolecule	Name: 23S rRNA / type: rna / ID: 2 / Number of copies: 1
Source (natural)	Organism: Pyrobaculum calidifontis JCM 11548 (archaea)
Molecular weight	Theoretical: 984.007875 KDa
Sequence	String: UAGGCAAAGC CGCCCGGUGG AUGGCUCGGC UCGGGCG(5MC)CG AAGAAGGGCG UGGCAAGCUG CGAUAAGCCC GGGGUA GC(B8T) GCAGGCAGGC UUAGAACCCG GGAUCCCCGA AUGGGGCUUC CUGCCGGGGC CGAAUAGGCC CCGGCGCCCC GUA AGGGGC ...String: UAGGCAAAGC CGCCCGGUGG AUGGCUCGGC UCGGGCG(5MC)CG AAGAAGGGCG UGGCAAGCUG CGAUAAGCCC GGGGUA GC(B8T) GCAGGCAGGC UUAGAACCCG GGAUCCCCGA AUGGGGCUUC CUGCCGGGGC CGAAUAGGCC CCGGCGCCCC GUA AGGGGC GGGAACGCGG GGAAAGGAAA CAUCUUAGUA CCCGCAGGAA GGGAAGCCAA CAGGGACCCC CUGAGUAGGG GCGA CCGAA AGGGGGAUAG CCCAAACCAA AUCCUCGCGG GACAACCGUG GGGAGAUGUG GGGCUUGGGC CCGGGCAACC GCCGG CGGG CGGUAGCCGA AGUGGGCUGG AAUGCCCCGC CGUAGAGGGU GAUAGCCCCG UAGGCGAAAC CGCCCGUGGC GGAGUC CCG GGGUCCCGGA GUACCUCGGC UUAGUUUUGC CGGGGGAACG CGCCGGCCAC UGGCCGGCAA GGCUAAGCAC GUCCCGA GU CCGAUAGCGC ACUAGUA(OMC)(OMC)G UGAGGGAAAG CUGAAAAGAA CCCCGGAAGG GGGGUGAAAA GAGCCUGAAA CCGGGCGGC UACAGUGGGG CAGGCCCGAA AGGAUGCCCC CUCCCGAAGG AAACCCCGGU GACGGGGGAG UACGAGGGAG G GGGUCCAG GGUCUGCCCU UACGUCUAGA AACACGGGCC GGGGAGUUCA (OMC)GGCCGUGGC GAGCCUAAGG GGUUCAAC C CCGGAGGCGU AGGGAAACCG ACAGCCCGCA GCGGGGCAAC CCGCGAGGGG CGGGGUCUUA AAGGGCCCGU AGUCACGGC CGUGAGACCA GAAACCGGGC GAUCUAGCCC UGGGCAGGGU GAAGCGGGGC GAAAGCCCCG UGGAGGCCCG AAGGGGUUCU GAUGUGCAA AU(OMC)GU(OMU)CCCA UGACCUGGGG CUAGGGGCAA AA(OMG)ACCAA(OMU)C AAGCCCGGUG AUAGC UGGU UCCCCCCGAA GCGGGUCUCA GCCCGGCCUC CCCGGAGGCG GCCGGCGGGG UAGAGUACUG AUCGGGGGUG CGGGAG CCG AAAGGCUCCG GCCCCCGGUC AAACUCCGAA CCUGCCAGCG CCGUAGAAGG GGGGAGGCGG GGGCGGUGGG GUAAGCC UC CGCUCCGAGA CGGGAACAAC CGAGACCGGG GUUAAGGCCC CCAAGUGCGG GCUUAGUGUC AAUCUAAAAG GGCGUCCC C CGCCCAAGAC AGCGGGGCCG UGGGCCUAAC AGCAGCCAUC GGCUAAGCAA CGCGUAACAG CGGACCCGCC GAGGCGGGG GGCCCCGAAG AUGUACAGGG ACUAAGCCCG CCGCCGAGAC CCCGGCCCGC GGGCCGUUGG CCCGCGUGGG GUAGGGGGGC GCGGCCGUG GGGCAGAAGC CGGGCCGUGA GGUCCGGUGG ACCCGCGGCC GACGAAGAUC CCGGCGGUAG UAGCAGCGAA G AGGGGUGA GAAGCCCCUC CGCCGGAAAG GACCAGGGUU UCCUGGCAAC UUCAAUAGGC CAGGAGUUAG CCGGUCCUAA GG CGGGGCC UAAUAGGCAC CCGCCGAAAG GGAAACGGGU UAAUAUUCCC GUGCCGCGGG GGUAGGUUCU GCGGCAACGC AGG CCCCGU CCCCGACGCC UCGGGAUAGG GCGGGCGGGA CUGCCGUCCC GCUUAACCGU CGAAGGCCGG GGAGUGCCGU AAUG GCGAG AACCGGCCGA AGGCGGGAAU AGCCGGGGGU UUCCCCGGUC CGCCCGACUC CUGGGGCCCG UGAAAAGGGG ACGGG GAAC GAGCCCCCGC GCCCGUACCG AGAACCGACG CAGGUGCUCC UGGGUGAGAA GCCCAAGGCG GCUCGGGUGA CCCCGG GCC AGGGAACU(OMC)G GCAAAUUGGC CCCGUAACUU CGGGAGAAGG GGUGCCUGCG GUCUUGGGGU AUACCCCCGG GAC CGCAGG UCGCAGUGGC AAGGGGGACC UGAC(PSU)GUUUA ACAAAAACAU AGGUCCCCGC GAGCCCGUAA (OMG)G (OMG)UGUGUA C(OMG)GGGGCUGA AUCCU(OMG)GCCA (OMC)UGGCGGUAC G(PSU)GA(A2M)CCCCG GGUACAACC GGGCGA(A2M)GCG C(4AC)(OMG)(OMC)UGAAGG CCGGGGGUAA CUCUGACCC(PSU) CUUAAGGUAG C(5MC)AA (A2M)UGCC UU(OMG)CCGG(OMG)UA AGU(OMU)CCGGCG UGCA(OMU)GAAUG GAUCAACGA(OMG) (OMG)UCCCCA CU GU(OMC)(OMC)CGGCCC GGGGCCCGGC GAACCCACCU (OMC)CAGGUGCAC AG(OMU)CCUGGGA CCCCCGACGG GG C(OMG)AGAAG UCCCUAUGGA GCUUCACAGC AGCCUGUCGU UGCGGGGGGG CGGGGGGUGC AGAGCGUAGG UGGGAGCGA UGAAACGGGG UCUCCGGGCC CCGUGGAUGC GACCCUGGAA CACCACCCAC UCUCCGCCCC UCCGCUUACC CGCCGCAAGG CGGGGACAG CGGCAGGCGG GCUGUUCGGC UG(OMG)GGC(OMG)GCA CACCCCUGAA AAGAUAUC(OMG)G GGGUGCCCA AAGCUCGGC(OMU) CAGGCGGGUC AGAAAUCCGC CGUAGAGUGU AAGGGCAAAA GCCGGGCUGA CUGGGCCCUU GAACGC AAG GGGCCCAGGC GGGAAACCGG GGCCUAGAGA ACGCUCGUGC CCCCACCAGU GGGGGCCGG(OMG) (OMC)AUGACAGA AAAGUUAC(OMC)C UAGGAAUAAC CGGC(PSU)CG(OMU)CG CGGGUGAGAG UCCCCAUCGA CCCC(OMG)CGGUU (PSU)(OMG)G(PSU)ACCCA GACGUCG(OMU)(OMC)(PSU) CU(OMU)CCCAUCC UGGCGGUGCA GCAGCCGCCA AGG GUGGGG C(OMU)(OMG)CCCGCCC AUUAAAGGGG AACGUG(A2M)GAU GGG(UR3)UCAGA(OMC) CG(OMU)CGCGAGA CAGGU(OMC)GGU CUCUACCUGU CGGGGGCGCU GGCCGCCUGA GGGGAAGGUG CCCUCAGUAC GAGAGGAACG GGGCGCC GC GGCCUCUAGU GUACCGGUUG UCCGGCAGGG CACUGCCGGG CAGCCACGCC GUGGGGGA(OMU)A ACCGCUGAAA GCAU CUAAG CGGGAAGCCC UC(OMC)(OMC)CGAGAC GAGGCGGCCG UUGCCCUGGG GGCAACCCCG GGGCACGAGG GCUCC (B8T)GUA GAAGACGGGG UUGAUGGGGG GGCGGUGUAA CCCCCGAGGG UUUCCCGAGG GGAGAGCCGG CCCCUCCCAA UC GCCCGAG CGU(G7M)C

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Macromolecule #3: 16S rRNA

Macromolecule	Name: 16S rRNA / type: rna / ID: 3 / Number of copies: 1
Source (natural)	Organism: Pyrobaculum calidifontis JCM 11548 (archaea)
Molecular weight	Theoretical: 487.519906 KDa
Sequence	String: AAAC(4AC)G(OMG)UUG AUCC(OMU)GCC(OMG)G ACCCGACCGC UAUCGGGGU(A2M) GGGCUAAGCC AUGCGAGUCG CGCGCCCGG GGGCGCCCGG GAGCGGCGCA CGGCUCAGUA ACACGUGCCU AACCUAACCU CGGGAGGGGG ACACCCCCGG G AAACUGGG ...String: AAAC(4AC)G(OMG)UUG AUCC(OMU)GCC(OMG)G ACCCGACCGC UAUCGGGGU(A2M) GGGCUAAGCC AUGCGAGUCG CGCGCCCGG GGGCGCCCGG GAGCGGCGCA CGGCUCAGUA ACACGUGCCU AACCUAACCU CGGGAGGGGG ACACCCCCGG G AAACUGGG GUCAAUCCCC CAUAGGGGAA GGGCGCUGGA AGGCCCCUUC CCCGAAAGGG CUGGCGGCCG AUCCGCCGCC AG UCGCCCG AGGGUGGGGG CACGGCCCAU CAGGUAGUUG GCGGG(PSU)UAAA GGCCCGCCAA GCCGAAGACG GGUAGGGGC GGUGAGAGCC GCGAGCCCCG AGAUGGGCAC UGAGACAAGG GCCCAGGCCC UACGGGGUGC AGCAGGCGCG AAUACUCCGC AAUGCGGGC AACCGCGACG GGGCUACCCC GAGUGCCGGG CGAAGAGCCC GGCUUUUGCC CGGUCUAAAA AGCCGGGAGA A UAAGC(OMG)GG (OMG)G(OMG)CAAGUCU (OMG)GUGUCAGCC GCCG(OMC)GGUAA U(A2M)CCAGCUCC GCG(M7A) GU(OMG)GU (OMC)GGGGUGUUU ACUGGGCUUA AAGCGCCCGU AGCCGGCCCG GCAAGUCGCU CCUGA(A2M)AU(OMC) CCCGGGCUCA ACCCGGGGGC CGGGGGCGAU ACUGCCGGGC UAGGGGGCGG GAGAGGCCGC CGGUACUCCG GGGGUAGGGG CGAAAUCCG UUAAUCCCCG GA(OMG)GACCACC AGUGGCGAAA GCGGGCGGCC AGAACGCGCC CGACGGUGAG GGGCGAA AG CCGGGGGAGC AAAGGGGAUU AGAUACCCCU GUAGUCCCGG CCGUAAACGA UGCGGGCUAG CUGUCGGUCG GGCUUAGG G CCCGGCCGGU GGCGAAGGGA AACCGUUAAG CCCGCCGCCU GGGGAGUACG GCCGCAAGGC UGAAACU(OMU)A(A2M) (A2M)GGAAUUGG CGGGGGGGCA CCACAAG(OMG)G(OMG) UGAAGCUUGC GGCUUAAUUG GAGUCAACGC CGGAAACCU CACCCGGGGC GACAGCAGGA UGAAGGCCAG GCUAACGACC UUGCCGGACG AGCUGAGAGG AGGUGCAUGG CCGUCGUCAG CUCGUG(OMC)(B8T)G UGAGGUGU(OMC)C GGUUAAGUCC GGC(A2M)ACGAGC GAGACCCCCG CCCCUAGUUG CUACC CCUC CCUACGGGGA GGGGGGCACA CUAGGGGGAC UGCCGGCGUA AGCCGGAGGA AGGAGGGGGC CACGGCA(OMG)GU CA GUAUGCC CCGAAACCC(OMC) GGGGCUGCAC GCGAGCU(OMG)CA AUGGC(OMG)(OMG)(OMG)GA CAGCGGGAUC CGA CCCCGA AAGGGGGAGG CAAUCCCGUA AACCCCGCCC CAGUAGGGAU CGAGGGCUGC AACUC(OMG)CCCU CGUGAACGUG GAAUCCCUA GUAAC(4AC)GCGU GUCACCAACG CGCGGUGAAU ACGUCCCUGC CCCUUGCACA CACCG(OMC)CCGU CGC ACCACC CGAGGGAGCC CUCUGCGAGG CCCCUCGCCG CAAGGUGGGG GGACGAGCAG GGGGCUCCCA AGGGGGGUGA AGUC GUA(6MZ)C AAGGUAGC(B8T)G UAGGGG(MA6)(MA6)CC UGCGGCUGGA UCACCUCC GENBANK: GENBANK: NR_040922.1

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Macromolecule #4: Large ribosomal subunit protein uL2

Macromolecule	Name: Large ribosomal subunit protein uL2 / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Pyrobaculum calidifontis JCM 11548 (archaea)
Molecular weight	Theoretical: 26.240348 KDa
Sequence	String: MGKRILVQRR GRGGSQFRSP SWKRDGPVRY PPNISGRGIV VEILHEPGLN APVAKIRMEN GVEFFNYAAE GLYVGQVIQV GPDAPPAVG NVLPLGKIPE GTMVFNVEKR FGDGGKFARS GGTYALVIGQ RPEENKTIVR LPSGRVIEVD ARGRATIGIV A GGGRVEKP ...String: MGKRILVQRR GRGGSQFRSP SWKRDGPVRY PPNISGRGIV VEILHEPGLN APVAKIRMEN GVEFFNYAAE GLYVGQVIQV GPDAPPAVG NVLPLGKIPE GTMVFNVEKR FGDGGKFARS GGTYALVIGQ RPEENKTIVR LPSGRVIEVD ARGRATIGIV A GGGRVEKP FVKAGKKYHR ARAKSWKYPT VRGKAMSPYA HPHGGGSHQK GGTPVPKTAP PGQKVGFIGS RCTGRGCVRA RA QQKQ UniProtKB: Large ribosomal subunit protein uL2

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Macromolecule #5: Large ribosomal subunit protein uL3

Macromolecule	Name: Large ribosomal subunit protein uL3 / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Pyrobaculum calidifontis JCM 11548 (archaea)
Molecular weight	Theoretical: 37.240543 KDa
Sequence	String: MGLKINRPRR GSMGVYPRKR AADIVPRVRT WPEVNLGKPT LLGFAAYKAG MLHAVVVDDR PTSPLYGKEV VKAVTVLDAP PLYVAAVRL YTLDPTNGYK VAVGEAWVSE PPADLRRVLT LPEKFDTEKQ LKALEEYRDV AVDVRVLVAT QPRLSGIGKK T PEVLEIPV ...String: MGLKINRPRR GSMGVYPRKR AADIVPRVRT WPEVNLGKPT LLGFAAYKAG MLHAVVVDDR PTSPLYGKEV VKAVTVLDAP PLYVAAVRL YTLDPTNGYK VAVGEAWVSE PPADLRRVLT LPEKFDTEKQ LKALEEYRDV AVDVRVLVAT QPRLSGIGKK T PEVLEIPV GGVPSIDERI NFAISLLGKT VSPKDVFTPG QLVDVIAVTK GKGYQGVVKR FGVTILPRWH KHRKGHRRTG TI GPQAPAL MFTQPRPGQM GFHQRTEYNK RILKIGDNGA EITPKSGFPH YGVIKGPYIL LQGSVPGARK RLVVLRYPVR PPK KAPPAA EPQVVWVSSQ SI UniProtKB: Large ribosomal subunit protein uL3

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Macromolecule #6: Large ribosomal subunit protein uL4

Macromolecule	Name: Large ribosomal subunit protein uL4 / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Pyrobaculum calidifontis JCM 11548 (archaea)
Molecular weight	Theoretical: 31.602059 KDa
Sequence	String: MGAVVKELLK FKLLDLSPYI KPAEERPPEA LKVYDVNGQY MADIETPIHF YEPVRPDLIR RAYLSALSAR FQPKGVYEGA GKEHSCESF GVGLGIARIP RYKGHLWPRG CFAPNTRGGR RAHPPRPEKK LHEEINWKEK NLAIRSAIAA TAYKSWVAAR G HMVEKVPS ...String: MGAVVKELLK FKLLDLSPYI KPAEERPPEA LKVYDVNGQY MADIETPIHF YEPVRPDLIR RAYLSALSAR FQPKGVYEGA GKEHSCESF GVGLGIARIP RYKGHLWPRG CFAPNTRGGR RAHPPRPEKK LHEEINWKEK NLAIRSAIAA TAYKSWVAAR G HMVEKVPS LPLVVSGDAE KIAKAKEAKK LFEVLGLWPD VERAAEGVKI RAGKGKMRGR RYKEPKSVLV VVSELDVPLI GA VRNFPGV DVVPVSHLNM LVLAPGGVPG RLTLWTATAV ERLKGLFL UniProtKB: Large ribosomal subunit protein uL4

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Macromolecule #7: Large ribosomal subunit protein uL5

Macromolecule	Name: Large ribosomal subunit protein uL5 / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Pyrobaculum calidifontis JCM 11548 (archaea)
Molecular weight	Theoretical: 20.156908 KDa
Sequence	String: MKWKELVLVK DHPMKRVYIE KVVVNIGVGT GGERLEKAAN LLRELTGAEP SLRRAKRSIK DFGIRKGEPI GVAVTLRRDK AVEFLMRAL QAVGNRIKRS SFDERGNVCF GIKEHIMLPG VKYDPAVGIW GMDVCVRLAK PGLRVQLRRR RRSKVGKGQL V TREEAVEF FQKVLGVQVD UniProtKB: Large ribosomal subunit protein uL5

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Macromolecule #8: Large ribosomal subunit protein uL6

Macromolecule	Name: Large ribosomal subunit protein uL6 / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Pyrobaculum calidifontis JCM 11548 (archaea)
Molecular weight	Theoretical: 21.663471 KDa
Sequence	String: MHVVYAVEEV PIPDGVKVAI EKTGPFDYVV KVKGPLGELV KEFKNTPVIM SLSDGKVVLE VLNAKKREYA LLGTYKGILK NMFLGVTKG WRYKLKVIYT HFPMLVKVQG NQLTIENFLG RKSKIVLEIP KGVKVEVKGK EDIVVEGIDR ELVSQFAAAI Q AATELRGE ...String: MHVVYAVEEV PIPDGVKVAI EKTGPFDYVV KVKGPLGELV KEFKNTPVIM SLSDGKVVLE VLNAKKREYA LLGTYKGILK NMFLGVTKG WRYKLKVIYT HFPMLVKVQG NQLTIENFLG RKSKIVLEIP KGVKVEVKGK EDIVVEGIDR ELVSQFAAAI Q AATELRGE EKPSPHGREG GLGVVDGIYV VGYEHVKA UniProtKB: Large ribosomal subunit protein uL6

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Macromolecule #9: Large ribosomal subunit protein eL8

Macromolecule	Name: Large ribosomal subunit protein eL8 / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Pyrobaculum calidifontis JCM 11548 (archaea)
Molecular weight	Theoretical: 16.013695 KDa
Sequence	String: MAVTIDPKTF YANPLPGKPF YVRFEVPSDV AEKALEILSI ARQTGKIKKG TNETTKAVER GLAKLVLIAE DVDPPEVVAH LPLLCEEKK VPYVYVPSKE KLGKAAGINV AAAAAVVIEA GQAAGELEAL VNKINEIRAK HGLNAIPVRR UniProtKB: Large ribosomal subunit protein eL8

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Macromolecule #10: Large ribosomal subunit protein uL13

Macromolecule	Name: Large ribosomal subunit protein uL13 / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Pyrobaculum calidifontis JCM 11548 (archaea)
Molecular weight	Theoretical: 21.736996 KDa
Sequence	String: MIVKKVLDLS QIPEKGEIVI DAEGHIMGRL ASYVAKILLS KPELRVVVVN AEKLVVTGDR KMVVEWFMRK ISEWRTHYNP EKAGPKIPR RPDRVFKRVV RGMLPKKVES GRDALKRLRV YMSIPLDFIQ RRRLVLYEVP AAKLRVRPLM QFVTLEEVWR S IDPAAWEK WNKAKEVWAK KIKQATSG UniProtKB: Large ribosomal subunit protein uL13

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Macromolecule #11: Large ribosomal subunit protein eL13

Macromolecule	Name: Large ribosomal subunit protein eL13 / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Pyrobaculum calidifontis JCM 11548 (archaea)
Molecular weight	Theoretical: 17.939125 KDa
Sequence	String: MIPKPVVQVP RSIKSGGVTK WKEGRGFSLG ELKEVGLNAE QARLLGLPVD TRRDSVWPQN VEALRKWLIE VLEGKATPPE PTLPKVVKV KRKRGRAFRG LTSAGRRSRG LISTRFRETH NYKFKKKAKE RRLKKRHEAT KGLGNVLRIS RIINESKE UniProtKB: Large ribosomal subunit protein eL13

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Macromolecule #12: Large ribosomal subunit protein uL14

Macromolecule	Name: Large ribosomal subunit protein uL14 / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Pyrobaculum calidifontis JCM 11548 (archaea)
Molecular weight	Theoretical: 15.768449 KDa
Sequence	String: MAKRGGKRTV GVPYRFHVTP GIFMNSLVPV ADNSGAKLVR VIGVVGHYSK SVHRRIPGAG VGDMVVVVVR EGKPELRKQI FRAIVVRQR RPYRRPDGTW VAFEDNAVVI VTPEGDPKGS EIHGPVAMEA TLRWPTIANL ASIVV UniProtKB: Large ribosomal subunit protein uL14

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Macromolecule #13: Large ribosomal subunit protein eL14

Macromolecule	Name: Large ribosomal subunit protein eL14 / type: protein_or_peptide / ID: 13 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)	Organism: Pyrobaculum calidifontis JCM 11548 (archaea)
Molecular weight	Theoretical: 11.470542 KDa
Sequence	String: MVKVIDVGRV VVKVLGREAG RKAVVVDIVD ENYVVITGPK SLSGVRRRRV NINHIEPTDK KIEIKKGASD EEVLKALEAA GLVEYMKER VKPKLFEITP ADVK UniProtKB: Large ribosomal subunit protein eL14

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Macromolecule #14: Large ribosomal subunit protein uL15

Macromolecule	Name: Large ribosomal subunit protein uL15 / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Pyrobaculum calidifontis JCM 11548 (archaea)
Molecular weight	Theoretical: 17.52543 KDa
Sequence	String: MVRRFKRATK YRRGSRTHGW GRVGQHRKSG GSGGKGMVGF HKHKWSLVMK YGESGTGWPF YGKHGFKQPE TISVEWRPIN VGRLAELVK ELRERGEVRE EEGRLVVDLL KLGYNKLLGG GHMDVPLVVY TPVASKAAVE KIAKAGGEVR IVSLVHR UniProtKB: Large ribosomal subunit protein uL15

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Macromolecule #15: 50S ribosomal protein L15e

Macromolecule	Name: 50S ribosomal protein L15e / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Pyrobaculum calidifontis JCM 11548 (archaea)
Molecular weight	Theoretical: 22.623768 KDa
Sequence	String: MPSAYQYMAM WYRKVGREVH ERLMRSVLIK WRKEPAIVRV ERPFRLERAR KLGYKPKQGV VVARVRLIRG PFNRRRPDSG RRPKRMGVY GITAWKSWRQ VAEERAARKF PGLEVLNSYW VADDGIYRYF EVILIDCNQP TIQRDPLYSG ICGREVPRRR L ARRLRERQ RKLIEHLKKT LLPKLKGTST A UniProtKB: 50S ribosomal protein L15e

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Macromolecule #16: Large ribosomal subunit protein uL16

Macromolecule	Name: Large ribosomal subunit protein uL16 / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Pyrobaculum calidifontis JCM 11548 (archaea)
Molecular weight	Theoretical: 19.89323 KDa
Sequence	String: MPARPARCYR RIKGPPYTRE EYIHGAPMIQ IPKFDMGTTS AAARAAFTMV AKLVAEERGQ IRMQALEAAR QISSKYLTKY VGDANYYLR LNVVPHHVLR ENRMLAMAGA DRLQEGMRLA FGSPAGRAAR VEPGQVIFYV EFKPEHLAHV KEALRRAASK L PIPTRIVV EPKGDGKTAS UniProtKB: Large ribosomal subunit protein uL16

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Macromolecule #17: Large ribosomal subunit protein uL18

Macromolecule	Name: Large ribosomal subunit protein uL18 / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Pyrobaculum calidifontis JCM 11548 (archaea)
Molecular weight	Theoretical: 23.424332 KDa
Sequence	String: MARGARYKVP FRRRREGVTN YRKRRKLLLS RKPRLVVRKT NKHLIAQIVI ARPHGDETVV GLDTRVLSKF GWKGDENNTS AAYLLGLIV GYKARAKGIK EAILDIGLHR PTPGARVFAV MKGVIDAGVE VPHGEEVVPS EERIRGEHVA EYAKRLKEEA P EAYKARFS ...String: MARGARYKVP FRRRREGVTN YRKRRKLLLS RKPRLVVRKT NKHLIAQIVI ARPHGDETVV GLDTRVLSKF GWKGDENNTS AAYLLGLIV GYKARAKGIK EAILDIGLHR PTPGARVFAV MKGVIDAGVE VPHGEEVVPS EERIRGEHVA EYAKRLKEEA P EAYKARFS RYLQRGLPPE ELPSHFEEVK KKIVEYYEQK LAKAVAR UniProtKB: Large ribosomal subunit protein uL18

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Macromolecule #18: Large ribosomal subunit protein eL18

Macromolecule	Name: Large ribosomal subunit protein eL18 / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Pyrobaculum calidifontis JCM 11548 (archaea)
Molecular weight	Theoretical: 13.224723 KDa
Sequence	String: MPPNPTGPTN RQLRMLIRFL RKAATANSAN IWRAIAELVE RPRRSRVVVN VGKLNRVAND GDVVVIPGKL LGGGELKKRV VVAAVSVTP KAWKKVVEAG GEVLTIPELV RRNPKGSGVK IVI UniProtKB: Large ribosomal subunit protein eL18

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Macromolecule #19: Large ribosomal subunit protein eL19

Macromolecule	Name: Large ribosomal subunit protein eL19 / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Pyrobaculum calidifontis JCM 11548 (archaea)
Molecular weight	Theoretical: 17.414342 KDa
Sequence	String: MVDVKRLAAE ILGVGVSRVK ISPEAVGRLE EVVTKDDVRA LIDEGLIWAE PEKGNSRGRW RELHEKKKKG RRRGQGSRKG RRLDEEEAW VNKMRVMRRF LNTLKRQGKL DPATWRQLYR MVKGNYFRDL RHLKTYINEH KLTKEPVK UniProtKB: Large ribosomal subunit protein eL19

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Macromolecule #20: Large ribosomal subunit protein eL20

Macromolecule	Name: Large ribosomal subunit protein eL20 / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Pyrobaculum calidifontis JCM 11548 (archaea)
Molecular weight	Theoretical: 8.929547 KDa
Sequence	String: MPKIYRVVGD TSTGMKFVVE VVGERPYDAL EKVYSVLGSR HKLKRTQIRI REVSVVEPSS AKSNEAKMLM VLEKVVRY UniProtKB: Large ribosomal subunit protein eL20

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Macromolecule #21: Large ribosomal subunit protein eL21

Macromolecule	Name: Large ribosomal subunit protein eL21 / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Pyrobaculum calidifontis JCM 11548 (archaea)
Molecular weight	Theoretical: 11.319301 KDa
Sequence	String: MVKRTHGYRY KSRKLLSKAP RERGMSGLSR LLYEYKPGDK VVIDIDPTFI STAPHRRYQG KVGVVIGTRG RAYVIETYIG DKKKIIITT PEHLRPHLGG UniProtKB: Large ribosomal subunit protein eL21

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Macromolecule #22: Large ribosomal subunit protein uL22

Macromolecule	Name: Large ribosomal subunit protein uL22 / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Pyrobaculum calidifontis JCM 11548 (archaea)
Molecular weight	Theoretical: 21.309219 KDa
Sequence	String: MPHFHYSLTD EQAAELVFKK YGVRITPEQI AKAYAPEQRM SWKKSVEVAR FIKGMTLKQA KAWLEDVVKL KRPIPIKTFK KKQAHHATP WEGWPVAKWP VKVAKRYLQL LENLENNAKF KGLDVERLVI IHAAAHKGIK IPNIMPRAFG RATPFNEETV N VEIVAAEL PKEVVPKRYK LNLVKR UniProtKB: Large ribosomal subunit protein uL22

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Macromolecule #23: Large ribosomal subunit protein uL23

Macromolecule	Name: Large ribosomal subunit protein uL23 / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Pyrobaculum calidifontis JCM 11548 (archaea)
Molecular weight	Theoretical: 9.307085 KDa
Sequence	String: MIKRFVITEK ALRLAERENK ITIIVERTAT KKQIADEVQR LYNVKVEKVN TLITPRGEKK AYVKLAEGYS AMDLLTKLGV L UniProtKB: Large ribosomal subunit protein uL23

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Macromolecule #24: Large ribosomal subunit protein uL24

Macromolecule	Name: Large ribosomal subunit protein uL24 / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Pyrobaculum calidifontis JCM 11548 (archaea)
Molecular weight	Theoretical: 14.588312 KDa
Sequence	String: MPFTASAQPR KQRLSLYAAP LHLRRKLLNA KLSPELQKKL GVKRLPVRRG DTVLIMRGDF KGVTGKVVKV DLKRVRIFVE GATRTNSRG QTVYYPIHPS KVMIVDVDLS DKARQKLIER RKRGQHGSS UniProtKB: Large ribosomal subunit protein uL24

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Macromolecule #25: Large ribosomal subunit protein eL24

Macromolecule	Name: Large ribosomal subunit protein eL24 / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Pyrobaculum calidifontis JCM 11548 (archaea)
Molecular weight	Theoretical: 7.10254 KDa
Sequence	String: MKIHKCSFCG ADIPPGYGIM YVRSDGTVQR FCSRKCFVSA VKYGRNPRKL AWVRKKKAKT SK UniProtKB: Large ribosomal subunit protein eL24

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Macromolecule #26: Large ribosomal subunit protein uL29

Macromolecule	Name: Large ribosomal subunit protein uL29 / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Pyrobaculum calidifontis JCM 11548 (archaea)
Molecular weight	Theoretical: 9.15887 KDa
Sequence	String: MSERKLKPKA LREMSPEERR ELLNQLRAEL VKLQTQRSRG FVEKPGRIRQ VRRMIARILT IERELAKAGA RAEKGGAKA UniProtKB: Large ribosomal subunit protein uL29

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Macromolecule #27: Large ribosomal subunit protein uL30

Macromolecule	Name: Large ribosomal subunit protein uL30 / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Pyrobaculum calidifontis JCM 11548 (archaea)
Molecular weight	Theoretical: 20.287805 KDa
Sequence	String: MMAATEQKTV YPRYAVIRLR GIPTTPGDIA ATLDLLRLRR KFTMVVVVGS PSVMGMIERV NDWVTWGEID ADTLAEVLRK RGRIVGDKP LTLEYLQKWG WNSFEEVALA YISGEIETLA CKKDFKAREG QRPPCIPYLK PFFRLHPPRG GLNSVKLHFS V GGDLGYRG PLINDLIKRM L UniProtKB: Large ribosomal subunit protein uL30

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Macromolecule #28: Large ribosomal subunit protein eL30

Macromolecule	Name: Large ribosomal subunit protein eL30 / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Pyrobaculum calidifontis JCM 11548 (archaea)
Molecular weight	Theoretical: 10.886839 KDa
Sequence	String: MIDIGRELQV AINTGKVIIG LRETKKSLLT GAPKLVIIAA NAPRWAREDI EYYAKLAGVP IFTFPGSSIE LGAAAKRPHK IMALAVVDP GQSEILKLVE HA UniProtKB: Large ribosomal subunit protein eL30

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Macromolecule #29: Large ribosomal subunit protein eL31

Macromolecule	Name: Large ribosomal subunit protein eL31 / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Pyrobaculum calidifontis JCM 11548 (archaea)
Molecular weight	Theoretical: 10.668632 KDa
Sequence	String: MSEEKKVVLT REYVINLRRT REVSRTKRAK YAVGLIRRFV ARHLKVEPKA VKLGQALNEA LWARSIEKPP RRVHVVVEKL DDGTVRVEL KQ UniProtKB: Large ribosomal subunit protein eL31

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Macromolecule #30: Large ribosomal subunit protein eL32

Macromolecule	Name: Large ribosomal subunit protein eL32 / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Pyrobaculum calidifontis JCM 11548 (archaea)
Molecular weight	Theoretical: 18.172572 KDa
Sequence	String: MVVVPRRELP QPLKRLLRLR LQMKRRKPEF VRIDQWRYKR IEDSGWRNQR TLDNKIRRKW KGWPKPVEVG YRKPAAVRGL HPSGFVEVL VHRPEDLAGL DPKVHAVRIG RTVGLRKRLE IVKKARELGF YVLNPGKEVV ELLKKELNTA QPQQ UniProtKB: Large ribosomal subunit protein eL32

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Macromolecule #31: Large ribosomal subunit protein eL34

Macromolecule	Name: Large ribosomal subunit protein eL34 / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Pyrobaculum calidifontis JCM 11548 (archaea)
Molecular weight	Theoretical: 9.373127 KDa
Sequence	String: MPRPAYRSRS LRRVKVKTPG GRTVVHYEKR AKGVPKCAVT GQPLGGMNSK VYRFGISTRA PSRPYGGVVS HKVLARAIRY AVRR UniProtKB: Large ribosomal subunit protein eL34

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Macromolecule #32: Large ribosomal subunit protein eL37

Macromolecule	Name: Large ribosomal subunit protein eL37 / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Pyrobaculum calidifontis JCM 11548 (archaea)
Molecular weight	Theoretical: 6.174278 KDa
Sequence	String: MKGTPSMGKH NKGKTHIRCP RCGRHSYNVT KKYCASCGWG RSKRWRRYNW AK UniProtKB: Large ribosomal subunit protein eL37

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Macromolecule #33: LSU ribosomal protein L38E

Macromolecule	Name: LSU ribosomal protein L38E / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Pyrobaculum calidifontis JCM 11548 (archaea)
Molecular weight	Theoretical: 7.954435 KDa
Sequence	String: MPKEIFLVEL WKKYGEKAQV VRVKRYEDFV KLKARLSGYL YTIRVPPDKA EQLISELKAK NVKVEEV UniProtKB: LSU ribosomal protein L38E

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Macromolecule #34: Large ribosomal subunit protein eL39

Macromolecule	Name: Large ribosomal subunit protein eL39 / type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Pyrobaculum calidifontis JCM 11548 (archaea)
Molecular weight	Theoretical: 6.030298 KDa
Sequence	String: MARNKPLGKK LRLAAALNSN RNPPLWVIAK TKRRVTRSPA RRHWRRQKLK A UniProtKB: Large ribosomal subunit protein eL39

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Macromolecule #35: Large ribosomal subunit protein eL40

Macromolecule	Name: Large ribosomal subunit protein eL40 / type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Pyrobaculum calidifontis JCM 11548 (archaea)
Molecular weight	Theoretical: 6.356657 KDa
Sequence	String: MPITLDPEKL AIVLQHRFNY KICRNCGARN PPDAEKCRRC RSRNLRPKKF KKK UniProtKB: Large ribosomal subunit protein eL40

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Macromolecule #36: eL42

Macromolecule	Name: eL42 / type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Pyrobaculum calidifontis JCM 11548 (archaea)
Molecular weight	Theoretical: 10.60361 KDa
Sequence	String: MKFPKSVNMY CPRCNTYTQH SVSNYHGGQR RSLAEGQRRY ERKLKGYGST PKPKQKRFAK VNKKVTLVFT CTKCGYKIVK SLGRMKKVE LV

+
Macromolecule #37: Large ribosomal subunit protein eL43

Macromolecule	Name: Large ribosomal subunit protein eL43 / type: protein_or_peptide / ID: 37 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Pyrobaculum calidifontis JCM 11548 (archaea)
Molecular weight	Theoretical: 11.387464 KDa
Sequence	String: MPFSHTKIVG PAGRYGARYG MGIRRKVTTI EVKQRGKHRC PSCRSLVRLE RLAFGIWHCP KCGHTFAGGA YVPQTILGKT IAPEELKGV EAEKAKWKEA AKR UniProtKB: Large ribosomal subunit protein eL43

+
Macromolecule #38: DJ-1/PfpI domain-containing protein

Macromolecule	Name: DJ-1/PfpI domain-containing protein / type: protein_or_peptide / ID: 38 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Pyrobaculum calidifontis JCM 11548 (archaea)
Molecular weight	Theoretical: 20.956449 KDa
Sequence	String: MPKALLYVID TAKRDEYPTL KNFLVKGGYE VDVAVGSRDV NVNYTVDLMT LPSDKLPTLF EEYDAFALAG GFKIYYHVLQ KKPPLKIWD LNIDKERLAQ LVVEFNRRRK LLIAPMAVPA YVAQLGLLSG RHATVYPVTE LIKILRDNKV NFVNKQVVRS E NIITVKDI TTTSEKEFLA ALREKG UniProtKB: DJ-1/PfpI domain-containing protein

+
Macromolecule #39: PaREP1 domain containing protein

Macromolecule	Name: PaREP1 domain containing protein / type: protein_or_peptide / ID: 39 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Pyrobaculum calidifontis JCM 11548 (archaea)
Molecular weight	Theoretical: 10.938797 KDa
Sequence	String: MPLCSKWESL ITWAEKGGNS YKALEFREKL VECIVYTATE KVKKKKLDEA EELLKYGKEV AKKFNIEELN FHIALLEKEI SKIRQRRAQ AQTK UniProtKB: PaREP1 domain containing protein

+
Macromolecule #40: Small ribosomal subunit protein eS1

Macromolecule	Name: Small ribosomal subunit protein eS1 / type: protein_or_peptide / ID: 40 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Pyrobaculum calidifontis JCM 11548 (archaea)
Molecular weight	Theoretical: 24.74016 KDa
Sequence	String: MAEKQKAVAA QEKVAISKRD PWALKKWFSV YAPPYLGGVF LAEVPAAEAQ KLVKRTLEVT LYDITKDISH LPIKLRFQIH RVDGLNAST RFKGLELTRD YLRSLIRRGT SKIMAITDVK TKDGWVMRVA VLGVTTHRVG TAQKSAIRKR FIEVVTKKAA E ADIGQFLK ...String: MAEKQKAVAA QEKVAISKRD PWALKKWFSV YAPPYLGGVF LAEVPAAEAQ KLVKRTLEVT LYDITKDISH LPIKLRFQIH RVDGLNAST RFKGLELTRD YLRSLIRRGT SKIMAITDVK TKDGWVMRVA VLGVTTHRVG TAQKSAIRKR FIEVVTKKAA E ADIGQFLK EVLEGTLAAD LFIAGKKIAP MRKVEVAKIK VLRYPPEEER VAVKEVAAEA AASS UniProtKB: Small ribosomal subunit protein eS1

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Macromolecule #41: Small ribosomal subunit protein uS2

Macromolecule	Name: Small ribosomal subunit protein uS2 / type: protein_or_peptide / ID: 41 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Pyrobaculum calidifontis JCM 11548 (archaea)
Molecular weight	Theoretical: 23.829814 KDa
Sequence	String: MAEEAQYEYL VPLEKYLSAG VRLGTRLSNK YLEERGFIYA VRPDGLRIFD IKKIDERLRI AAKLIARYPP DRVLVHTTRP YGFKPVQMF CKFVGCKALT GRFIPGMLTN PNLSNYLEVD LLFVVDPRLD SQAVAEAAKM GIPVVALVDT DTPHQYIDFM I PCNNKGRK ...String: MAEEAQYEYL VPLEKYLSAG VRLGTRLSNK YLEERGFIYA VRPDGLRIFD IKKIDERLRI AAKLIARYPP DRVLVHTTRP YGFKPVQMF CKFVGCKALT GRFIPGMLTN PNLSNYLEVD LLFVVDPRLD SQAVAEAAKM GIPVVALVDT DTPHQYIDFM I PCNNKGRK SLALIFWVLA RQVLRERGEL KPDQDLPVPP EEFETRLVQT UniProtKB: Small ribosomal subunit protein uS2

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Macromolecule #42: Small ribosomal subunit protein uS3

Macromolecule	Name: Small ribosomal subunit protein uS3 / type: protein_or_peptide / ID: 42 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Pyrobaculum calidifontis JCM 11548 (archaea)
Molecular weight	Theoretical: 24.688248 KDa
Sequence	String: MSTAQRRLPV YKKILEENKK KWMIKEFLEY RLAKYGYIDS EILKTPLGTR IVIYAERPSR IIGRKGAIVK EVSSILANKL GVENPQIDV IDVSKIEAPE LFPKVVAYRI ANAMARGVRF RRVMFVAVRQ LMEAGAKGFE IVVSGKLSTE RAKFEKITYG K LYKIGYDA ...String: MSTAQRRLPV YKKILEENKK KWMIKEFLEY RLAKYGYIDS EILKTPLGTR IVIYAERPSR IIGRKGAIVK EVSSILANKL GVENPQIDV IDVSKIEAPE LFPKVVAYRI ANAMARGVRF RRVMFVAVRQ LMEAGAKGFE IVVSGKLSTE RAKFEKITYG K LYKIGYDA KNRVRRAVVH VLLKPGIYGI EVRITPATLR YSDEYKIKPP TRPEAAAQ UniProtKB: Small ribosomal subunit protein uS3

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Macromolecule #43: Small ribosomal subunit protein uS4

Macromolecule	Name: Small ribosomal subunit protein uS4 / type: protein_or_peptide / ID: 43 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Pyrobaculum calidifontis JCM 11548 (archaea)
Molecular weight	Theoretical: 18.516201 KDa
Sequence	String: MGGLKKPKKK YLAGKPKKIW NKQLLLEELQ LMGEYGLRNK KELWLARARL KWIVRRARAL LSMTAEERAP LEVPFKEKLY KMGFIEDPN VPLDRILSLD VRAILERRLQ TLVFRMGLAK SIHHARQLVV HGHVLVAGRR VTSPGFLVPR ELEDKITIEQ UniProtKB: Small ribosomal subunit protein uS4

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Macromolecule #44: Small ribosomal subunit protein eS4

Macromolecule	Name: Small ribosomal subunit protein eS4 / type: protein_or_peptide / ID: 44 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Pyrobaculum calidifontis JCM 11548 (archaea)
Molecular weight	Theoretical: 26.774389 KDa
Sequence	String: MVHLRKSLAP YWWPIPRKAG GVWAVRPSTG PHSLEYSLPL AVVVRDVLRY AKTLREARYI ISRGYIKVDG VVRKDYKFPI GLMDVVEIV PTGEIYRVVP DEAKYYDLKP IPSSEAALKP LRVEGKTAVS GGRIQLHFHD GRNLILPPDV GRQIKTFDTV V YDLENKAI ...String: MVHLRKSLAP YWWPIPRKAG GVWAVRPSTG PHSLEYSLPL AVVVRDVLRY AKTLREARYI ISRGYIKVDG VVRKDYKFPI GLMDVVEIV PTGEIYRVVP DEAKYYDLKP IPSSEAALKP LRVEGKTAVS GGRIQLHFHD GRNLILPPDV GRQIKTFDTV V YDLENKAI KTHLPLRLGQ LAVVTHGGNI GFVGQFFEVV WTLKRRQSVV ALRKGEEVRR TILDYIMVIG TEAPVVKIS UniProtKB: Small ribosomal subunit protein eS4

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Macromolecule #45: Small ribosomal subunit protein uS5

Macromolecule	Name: Small ribosomal subunit protein uS5 / type: protein_or_peptide / ID: 45 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Pyrobaculum calidifontis JCM 11548 (archaea)
Molecular weight	Theoretical: 22.153717 KDa
Sequence	String: MSVVDLSLWE PKTALGRMVK EGKIRTIDEI FANNLLIKEP EIVDILLPGL KQELLNLNLV QRQTHAGERN QFQAVVAVGN EDGYVGVGI GKSRQVRQAI EKATREAKLN ITPVRRGCGS WKCSCDEPHS VPFVVSGKSG SVEVTLIPAP KGVGLVAGDV A KVVLRLAG ...String: MSVVDLSLWE PKTALGRMVK EGKIRTIDEI FANNLLIKEP EIVDILLPGL KQELLNLNLV QRQTHAGERN QFQAVVAVGN EDGYVGVGI GKSRQVRQAI EKATREAKLN ITPVRRGCGS WKCSCDEPHS VPFVVSGKSG SVEVTLIPAP KGVGLVAGDV A KVVLRLAG IKDVWTKTRG DTRTTLNFAL AVFNALRNTY YFKL UniProtKB: Small ribosomal subunit protein uS5

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Macromolecule #46: Small ribosomal subunit protein eS6

Macromolecule	Name: Small ribosomal subunit protein eS6 / type: protein_or_peptide / ID: 46 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Pyrobaculum calidifontis JCM 11548 (archaea)
Molecular weight	Theoretical: 16.248015 KDa
Sequence	String: MPTFKLVLSD PLSGKARQFE IKDPLAQRFV GLRIGEEIDG TVLKELIELP KGVKIRITGG SGVEGAPMHP GVPGPVKRYI LADGPPGYR PRKRGMRKKK LVRGDTITDS IVQINAVLVY PEGYSGPPAI PLGAKELEKL KGGKTEEAAA SQ UniProtKB: Small ribosomal subunit protein eS6

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Macromolecule #47: Small ribosomal subunit protein uS7

Macromolecule	Name: Small ribosomal subunit protein uS7 / type: protein_or_peptide / ID: 47 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Pyrobaculum calidifontis JCM 11548 (archaea)
Molecular weight	Theoretical: 25.515701 KDa
Sequence	String: MASIFGEQLP KTSRVEYIGD VPIIEECPRE LKTLDGNPIL LFGKWSFEDV VVRDPGLRRY ICLKPVVLPH TEGRYQKRRF GKAHIPIVE RLVNLLMRPG RNTGKKHKAY NIVKRAFEII YYKTGRNPIQ VFIDAIINTA PREEITRIIM GGIAYSVSVD V SPQRRLDL ...String: MASIFGEQLP KTSRVEYIGD VPIIEECPRE LKTLDGNPIL LFGKWSFEDV VVRDPGLRRY ICLKPVVLPH TEGRYQKRRF GKAHIPIVE RLVNLLMRPG RNTGKKHKAY NIVKRAFEII YYKTGRNPIQ VFIDAIINTA PREEITRIIM GGIAYSVSVD V SPQRRLDL SLRWITEGAR QCSFNNPKPI EECLADEIMA AAANDPKSYA VRKREELERI AAASR UniProtKB: Small ribosomal subunit protein uS7

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Macromolecule #48: Small ribosomal subunit protein uS8

Macromolecule	Name: Small ribosomal subunit protein uS8 / type: protein_or_peptide / ID: 48 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Pyrobaculum calidifontis JCM 11548 (archaea)
Molecular weight	Theoretical: 14.836492 KDa
Sequence	String: MVMLDLLSNA LTAIKNAEAM GKRQVVIWPV NKLIYYTLRV LQRYGYVGEI EYIDDGRGGK YVVQLLGKIN DIGAIRPRFP VKYREIVEW EQKYLPARQI GILVISTSQG VMSHLEAKEK KIGGVLLAYV Y UniProtKB: Small ribosomal subunit protein uS8

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Macromolecule #49: Small ribosomal subunit protein eS8

Macromolecule	Name: Small ribosomal subunit protein eS8 / type: protein_or_peptide / ID: 49 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Pyrobaculum calidifontis JCM 11548 (archaea)
Molecular weight	Theoretical: 14.446925 KDa
Sequence	String: MKLGAFYKGG DLVKPSGGKK GRVRKTKKKA LGGGPPQIPK LGEEDIRYVE RVRGGNHKVR LRQARYANVY IPKERRHVKA KILSIVSSP SNPDYARRNY IVKGAIIQTE VGKAVVTSRP GQDGVINAVL IE UniProtKB: Small ribosomal subunit protein eS8

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Macromolecule #50: Small ribosomal subunit protein uS9

Macromolecule	Name: Small ribosomal subunit protein uS9 / type: protein_or_peptide / ID: 50 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Pyrobaculum calidifontis JCM 11548 (archaea)
Molecular weight	Theoretical: 16.146151 KDa
Sequence	String: MPWVSILQEN PRVIISVGKK KTAVARAIIK PGMGRVRING YPLELWPIEM ARVKMQEPLI LAGDLAKKVD IEVNVRGGGY MGQAIATRI AIARGLVAYF QSEELKKLYE EYDPYMLKGD PRRTEPKKPG IKHARSKRQK AYR UniProtKB: Small ribosomal subunit protein uS9

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Macromolecule #51: Small ribosomal subunit protein uS10

Macromolecule	Name: Small ribosomal subunit protein uS10 / type: protein_or_peptide / ID: 51 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Pyrobaculum calidifontis JCM 11548 (archaea)
Molecular weight	Theoretical: 12.221515 KDa
Sequence	String: MSLASRRKVR IRLYGTNPAD LDQVAREIVD LAKKMGVAVR GPIPLPTKRL MVTVRRAPSG QGYHTFDHWE LRISKRLIDI EASERVLRR LMTIRVPDTV KIELQLI UniProtKB: Small ribosomal subunit protein uS10

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Macromolecule #52: Small ribosomal subunit protein uS11

Macromolecule	Name: Small ribosomal subunit protein uS11 / type: protein_or_peptide / ID: 52 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Pyrobaculum calidifontis JCM 11548 (archaea)
Molecular weight	Theoretical: 14.984247 KDa
Sequence	String: MSTAETPQQP QQQQKLRWGI AWIYSSSNNT IITITDLTGA ETVARVSGGM VVRADKDKPS PWAAMQAAYK AAQLALARGI NAVHIKVRG PGGYGMKVPG PGASAAIRAL ARSGLVIGRI EDVTPIPH(IAS)T IRPPSGRKGR RV UniProtKB: Small ribosomal subunit protein uS11

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Macromolecule #53: Small ribosomal subunit protein uS12

Macromolecule	Name: Small ribosomal subunit protein uS12 / type: protein_or_peptide / ID: 53 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Pyrobaculum calidifontis JCM 11548 (archaea)
Molecular weight	Theoretical: 16.477486 KDa
Sequence	String: MPGKKSPYGL FAGGKLKKKR QKFRWNDVTY KRRMLGLAEK YDPLEGAPMA RGIVLEKVGV EARKPNAAVR KCVRVQLVKN GKVVTAFVP YDGGLNYINE HDEVIIERIG GPEGKSLGDI PGVRFKVVKV NGVSLWAIWR GKKQKPTR UniProtKB: Small ribosomal subunit protein uS12

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Macromolecule #54: Small ribosomal subunit protein uS13

Macromolecule	Name: Small ribosomal subunit protein uS13 / type: protein_or_peptide / ID: 54 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Pyrobaculum calidifontis JCM 11548 (archaea)
Molecular weight	Theoretical: 16.758621 KDa
Sequence	String: MAQEIRAIVR IGDTDLDGNK TVAYALAKIR GIGIATAYAI CRKLGIDPHA TLGLLPEDQI NKLDWAVRNL HELAPAWFLN RRKDPETGR DLHLIGSELV LAAKRDVDLM KKLKSWKGIR HSLGLKVRGQ RTVTTGRFGA TAGVTKKKAA PGGK UniProtKB: Small ribosomal subunit protein uS13

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Macromolecule #55: Small ribosomal subunit protein uS14

Macromolecule	Name: Small ribosomal subunit protein uS14 / type: protein_or_peptide / ID: 55 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Pyrobaculum calidifontis JCM 11548 (archaea)
Molecular weight	Theoretical: 6.429787 KDa
Sequence	String: MPSTKPPKER PYGKSKIRCL RCGTREAVIR KYGLYLCRRC FREVAPVLGF KKYY UniProtKB: Small ribosomal subunit protein uS14

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Macromolecule #56: Small ribosomal subunit protein uS15

Macromolecule	Name: Small ribosomal subunit protein uS15 / type: protein_or_peptide / ID: 56 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Pyrobaculum calidifontis JCM 11548 (archaea)
Molecular weight	Theoretical: 17.587598 KDa
Sequence	String: MPHRSRHKKG RSSSVRPPHP TVPTWIQYTP EEVEQLAVEL ARRGFPPSQI GIILRDQYGI PLVKPITGKK LTKILEEHGI KQELPEDLL NLIRRALRIR KHLEEHPKDM ASRRGLQLVE SKIHRLIKYY KRVGKIPQDF VYNPEALSHL AT UniProtKB: Small ribosomal subunit protein uS15

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Macromolecule #57: Small ribosomal subunit protein uS17

Macromolecule	Name: Small ribosomal subunit protein uS17 / type: protein_or_peptide / ID: 57 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Pyrobaculum calidifontis JCM 11548 (archaea)
Molecular weight	Theoretical: 16.986256 KDa
Sequence	String: MTVVKLQSRP KVGDVVTLPD GKKVEVKNIG VPYVLPPAAV CDDPLCPWHG HLKIRLKLLE VTVEKVRMHK AAVVTHEWVH YLPKYNRYE RRRRRMRVRV PECIEVKPGD KVIIAETRPL SKTIAWVVIG KKEDVVEWKA KHETLPSV UniProtKB: Small ribosomal subunit protein uS17

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Macromolecule #58: Small ribosomal subunit protein eS17

Macromolecule	Name: Small ribosomal subunit protein eS17 / type: protein_or_peptide / ID: 58 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Pyrobaculum calidifontis JCM 11548 (archaea)
Molecular weight	Theoretical: 8.082505 KDa
Sequence	String: MGRVKPKFIK SLAEKLLEAY PDRFTESFKE NKKAVAELAD IPSKTVRNKV AGYITRLVKR QKAKEKAEQA A UniProtKB: Small ribosomal subunit protein eS17

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Macromolecule #59: Small ribosomal subunit protein uS19

Macromolecule	Name: Small ribosomal subunit protein uS19 / type: protein_or_peptide / ID: 59 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Pyrobaculum calidifontis JCM 11548 (archaea)
Molecular weight	Theoretical: 18.182557 KDa
Sequence	String: MSSKEAEAQK GKQGWITPTV IPPEEWGSFR YRGKTLEELL NMPMDEFIKL LPARQRRSLK RGLKPEHRKL LEKIRKAKRL MAQGKKVTI KTHCRDMIIL PEMVGLTIHV YNGITYIPVF ISPWHIGHYL GEFALTTRIV QHGEPGLKAT RSSLHIAAK UniProtKB: Small ribosomal subunit protein uS19

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Macromolecule #60: Small ribosomal subunit protein eS19

Macromolecule	Name: Small ribosomal subunit protein eS19 / type: protein_or_peptide / ID: 60 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Pyrobaculum calidifontis JCM 11548 (archaea)
Molecular weight	Theoretical: 17.770932 KDa
Sequence	String: MTSVKDVPAD LLIAELAKYI KDNVPQVKPP VWAPFVKTGA NKERPPMSED WWYIRAASIM RKLYIYGPVG VGSLRTAYGY RAKIGDKTR PERTRKAGGA IIRKILQQLE HAGFVTKTKR GRVLTPEGKS LVDRVAARIA KELVKQRPEL AKYVAPPKE UniProtKB: Small ribosomal subunit protein eS19

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Macromolecule #61: Small ribosomal subunit protein eS24

Macromolecule	Name: Small ribosomal subunit protein eS24 / type: protein_or_peptide / ID: 61 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Pyrobaculum calidifontis JCM 11548 (archaea)
Molecular weight	Theoretical: 14.855409 KDa
Sequence	String: MSPVSEAQFN ILSLRENKLL GRKEVLVEIL HRKSSTPTRQ SVREWLAKQL GVEVDKIFVR KIKTEYGIGK SKAEVHVYTD GALARVIEP LYILARNLGE EGKKLLEEAR KRRNERREKR RRKKKGGGK UniProtKB: Small ribosomal subunit protein eS24

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Macromolecule #62: SSU ribosomal protein S25E

Macromolecule	Name: SSU ribosomal protein S25E / type: protein_or_peptide / ID: 62 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Pyrobaculum calidifontis JCM 11548 (archaea)
Molecular weight	Theoretical: 12.477728 KDa
Sequence	String: MGGKKRPTLS QLAKKAEKEK AQPQQQKSKK EVKKEEAPAK RTIQTLDEKV FQTIAKEVQS LKAVTPYEIA TKYGIKMSLA FKVLRALRD RGDLVLVAKG HRTEVYVPKR S UniProtKB: SSU ribosomal protein S25E

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Macromolecule #63: SSU ribosomal protein S26E

Macromolecule	Name: SSU ribosomal protein S26E / type: protein_or_peptide / ID: 63 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Pyrobaculum calidifontis JCM 11548 (archaea)
Molecular weight	Theoretical: 11.571864 KDa
Sequence	String: MAMPKKRKNR GRKKGDKGRE PYVYCDNCGK VVPRSKAIRL TVPYSPVPPD LARELEKQGA IISRYLVTKT YCINCAVFFG IIKVRPREE RKKRVPLQQV I UniProtKB: SSU ribosomal protein S26E

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Macromolecule #64: Small ribosomal subunit protein eS27

Macromolecule	Name: Small ribosomal subunit protein eS27 / type: protein_or_peptide / ID: 64 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Pyrobaculum calidifontis JCM 11548 (archaea)
Molecular weight	Theoretical: 7.446884 KDa
Sequence	String: MPQRFSRVLI PQPRSRFVRL RCPDCGNEQV TFSHAAMVVR CLVCGRVLAQ PTGGKAIFAG HVVKVLE UniProtKB: Small ribosomal subunit protein eS27

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Macromolecule #65: eS28

Macromolecule	Name: eS28 / type: protein_or_peptide / ID: 65 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Pyrobaculum calidifontis JCM 11548 (archaea)
Molecular weight	Theoretical: 8.575992 KDa
Sequence	String: MAEEVKFSPY EDAVAGLVVQ ILGRTGVAGE VTQVKIRVLE GRDKGRVLTR NVKGPVRLGD IVMLRETERE ARRITAR

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Macromolecule #66: SSU ribosomal protein S30E

Macromolecule	Name: SSU ribosomal protein S30E / type: protein_or_peptide / ID: 66 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Pyrobaculum calidifontis JCM 11548 (archaea)
Molecular weight	Theoretical: 6.113139 KDa
Sequence	String: MPSHGSLTKA GKVRNQTPKI PAKPRKNLTP RRRNIRNYKR RVLYASQQAA AAEA UniProtKB: SSU ribosomal protein S30E

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Macromolecule #67: aS35

Macromolecule	Name: aS35 / type: protein_or_peptide / ID: 67 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Pyrobaculum calidifontis JCM 11548 (archaea)
Molecular weight	Theoretical: 7.856398 KDa
Sequence	String: MAEELEAVAA KLITRLREAE RLALEGKTSE AKAILKEVLK EARERGLEKS LRNLLLRVKA LLRRKTRR

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Macromolecule #68: Small zinc finger protein HVO-2753-like zinc-binding pocket domai...

Macromolecule	Name: Small zinc finger protein HVO-2753-like zinc-binding pocket domain-containing protein type: protein_or_peptide / ID: 68 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Pyrobaculum calidifontis JCM 11548 (archaea)
Molecular weight	Theoretical: 6.956113 KDa
Sequence	String: MSIRGARIYG PSPPADSTTN WPLAPLERGV VFACPNCGKT TIVRSARARK LGVPYRCPEC GFVGP UniProtKB: Small zinc finger protein HVO-2753-like zinc-binding pocket domain-containing protein

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Macromolecule #69: SPERMINE

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Macromolecule #70: MAGNESIUM ION

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Macromolecule #71: ZINC ION

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Macromolecule #72: water

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Experimental details

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Structure determination

Method	cryo EM
Processing	single particle reconstruction
Aggregation state	particle

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Sample preparation

Concentration

0.12 mg/mL

Buffer

pH: 7
Component:

Concentration	Formula	Name
20.0 mM	C₈H₁₈N₂O₄S	HEPES
10.0 mM	KCl	Potassium Chloride
20.0 mM	Mg(CH₃COO)₂	Magnesium Acetate
3.0 mM	C₁₀H₂₆N₄	Spermine
2.0 mM	C₄H₁₀O₂S₂	DTT

Grid

Model: UltrAuFoil R1.2/1.3 / Material: GOLD / Support film - Material: CARBON / Support film - topology: CONTINUOUS / Pretreatment - Type: GLOW DISCHARGE

Vitrification

Cryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 277.15 K / Instrument: FEI VITROBOT MARK IV

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Electron microscopy

Microscope	TFS KRIOS
Image recording	Film or detector model: GATAN K3 (6k x 4k) / Number grids imaged: 1 / Number real images: 7318 / Average electron dose: 40.0 e/Å²
Electron beam	Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron optics	Illumination mode: OTHER / Imaging mode: BRIGHT FIELD / Nominal defocus max: 1.5 µm / Nominal defocus min: 0.5 µm / Nominal magnification: 105000
Experimental equipment	Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Particle selection	Number selected: 2360632
Startup model	Type of model: OTHER
Final reconstruction	Resolution.type: BY AUTHOR / Resolution: 2.36 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: cryoSPARC (ver. 4.4) / Details: Global resolution / Number images used: 129829
Initial angle assignment	Type: MAXIMUM LIKELIHOOD / Software - Name: cryoSPARC (ver. 4.4)
Final angle assignment	Type: MAXIMUM LIKELIHOOD / Software - Name: cryoSPARC (ver. 4.4)

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Atomic model buiding 1

Refinement	Protocol: AB INITIO MODEL
Output model	PDB-9e71: Cryo-EM structure of the Pyrobaculum calidifontis 70S ribosome

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Related info.:EMDB / PDB / SASBDB / Comparison of 3 databanks / Yorodumi Search / Aug 31, 2016. New EM Navigator & Yorodumi / Yorodumi Papers / Jmol/JSmol / Function and homology information / Changes in new EM Navigator and Yorodumi

Macromolecule	Name: SPERMINE / type: ligand / ID: 69 / Number of copies: 64 / Formula: SPM
Molecular weight	Theoretical: 202.34 Da
Chemical component information	ChemComp-SPM: SPERMINE

Macromolecule	Name: MAGNESIUM ION / type: ligand / ID: 70 / Number of copies: 252 / Formula: MG
Molecular weight	Theoretical: 24.305 Da

Macromolecule	Name: ZINC ION / type: ligand / ID: 71 / Number of copies: 11 / Formula: ZN
Molecular weight	Theoretical: 65.409 Da

Macromolecule	Name: water / type: ligand / ID: 72 / Number of copies: 7812 / Formula: HOH
Molecular weight	Theoretical: 18.015 Da
Chemical component information	ChemComp-HOH: WATER

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-Supplemental data

-Sample components

+Entire : 70S ribosome

+Supramolecule #1: 70S ribosome

+Supramolecule #2: 50S Subunit

+Supramolecule #3: 30S subunit

+Macromolecule #1: 5S rRNA

+Macromolecule #2: 23S rRNA

+Macromolecule #3: 16S rRNA

+Macromolecule #4: Large ribosomal subunit protein uL2

+Macromolecule #5: Large ribosomal subunit protein uL3

+Macromolecule #6: Large ribosomal subunit protein uL4

+Macromolecule #7: Large ribosomal subunit protein uL5

+Macromolecule #8: Large ribosomal subunit protein uL6

+Macromolecule #9: Large ribosomal subunit protein eL8

+Macromolecule #10: Large ribosomal subunit protein uL13

+Macromolecule #11: Large ribosomal subunit protein eL13

+Macromolecule #12: Large ribosomal subunit protein uL14

+Macromolecule #13: Large ribosomal subunit protein eL14

+Macromolecule #14: Large ribosomal subunit protein uL15

+Macromolecule #15: 50S ribosomal protein L15e

+Macromolecule #16: Large ribosomal subunit protein uL16

+Macromolecule #17: Large ribosomal subunit protein uL18

+Macromolecule #18: Large ribosomal subunit protein eL18

+Macromolecule #19: Large ribosomal subunit protein eL19

+Macromolecule #20: Large ribosomal subunit protein eL20

+Macromolecule #21: Large ribosomal subunit protein eL21

+Macromolecule #22: Large ribosomal subunit protein uL22

+Macromolecule #23: Large ribosomal subunit protein uL23

+Macromolecule #24: Large ribosomal subunit protein uL24

+Macromolecule #25: Large ribosomal subunit protein eL24

+Macromolecule #26: Large ribosomal subunit protein uL29

+Macromolecule #27: Large ribosomal subunit protein uL30

+Macromolecule #28: Large ribosomal subunit protein eL30

+Macromolecule #29: Large ribosomal subunit protein eL31

+Macromolecule #30: Large ribosomal subunit protein eL32

+Macromolecule #31: Large ribosomal subunit protein eL34

+Macromolecule #32: Large ribosomal subunit protein eL37

+Macromolecule #33: LSU ribosomal protein L38E

+Macromolecule #34: Large ribosomal subunit protein eL39

+Macromolecule #35: Large ribosomal subunit protein eL40

+Macromolecule #36: eL42

+Macromolecule #37: Large ribosomal subunit protein eL43

+Macromolecule #38: DJ-1/PfpI domain-containing protein

+Macromolecule #39: PaREP1 domain containing protein

+Macromolecule #40: Small ribosomal subunit protein eS1

+Macromolecule #41: Small ribosomal subunit protein uS2

+Macromolecule #42: Small ribosomal subunit protein uS3

+Macromolecule #43: Small ribosomal subunit protein uS4

+Macromolecule #44: Small ribosomal subunit protein eS4

+Macromolecule #45: Small ribosomal subunit protein uS5

+Macromolecule #46: Small ribosomal subunit protein eS6

+Macromolecule #47: Small ribosomal subunit protein uS7

+Macromolecule #48: Small ribosomal subunit protein uS8

+Macromolecule #49: Small ribosomal subunit protein eS8

+Macromolecule #50: Small ribosomal subunit protein uS9

+Macromolecule #51: Small ribosomal subunit protein uS10

+Macromolecule #52: Small ribosomal subunit protein uS11

+Macromolecule #53: Small ribosomal subunit protein uS12

+Macromolecule #54: Small ribosomal subunit protein uS13

+Macromolecule #55: Small ribosomal subunit protein uS14

+Macromolecule #56: Small ribosomal subunit protein uS15

+Macromolecule #57: Small ribosomal subunit protein uS17

+Macromolecule #58: Small ribosomal subunit protein eS17

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