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Yorodumi- EMDB-44254: Consensus map: CW Flagellar Switch Complex - FliF, FliG, FliM, an... -
+Open data
-Basic information
Entry | Database: EMDB / ID: EMD-44254 | |||||||||
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Title | Consensus map: CW Flagellar Switch Complex - FliF, FliG, FliM, and FliN forming the C-ring from Salmonella | |||||||||
Map data | ||||||||||
Sample |
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Keywords | Domain Swap / Symmetry mismatch / Flagellar component / Switch complex / MOTOR PROTEIN | |||||||||
Biological species | Salmonella enterica subsp. enterica serovar Typhimurium (bacteria) | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 7.01 Å | |||||||||
Authors | Singh PK / Iverson TM | |||||||||
Funding support | United States, 1 items
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Citation | Journal: To Be Published Title: Consensus map: CW Flagellar Switch Complex - FliF, FliG, FliM, and FliN forming the C-ring from Salmonella Authors: Singh PK / Iverson TM | |||||||||
History |
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-Structure visualization
Supplemental images |
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-Downloads & links
-EMDB archive
Map data | emd_44254.map.gz | 253.2 MB | EMDB map data format | |
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Header (meta data) | emd-44254-v30.xml emd-44254.xml | 12.4 KB 12.4 KB | Display Display | EMDB header |
FSC (resolution estimation) | emd_44254_fsc.xml | 17 KB | Display | FSC data file |
Images | emd_44254.png | 47.3 KB | ||
Masks | emd_44254_msk_1.map | 512 MB | Mask map | |
Filedesc metadata | emd-44254.cif.gz | 3.8 KB | ||
Others | emd_44254_half_map_1.map.gz emd_44254_half_map_2.map.gz | 474.6 MB 474.6 MB | ||
Archive directory | http://ftp.pdbj.org/pub/emdb/structures/EMD-44254 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-44254 | HTTPS FTP |
-Validation report
Summary document | emd_44254_validation.pdf.gz | 968.1 KB | Display | EMDB validaton report |
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Full document | emd_44254_full_validation.pdf.gz | 967.7 KB | Display | |
Data in XML | emd_44254_validation.xml.gz | 25.8 KB | Display | |
Data in CIF | emd_44254_validation.cif.gz | 34.7 KB | Display | |
Arichive directory | https://ftp.pdbj.org/pub/emdb/validation_reports/EMD-44254 ftp://ftp.pdbj.org/pub/emdb/validation_reports/EMD-44254 | HTTPS FTP |
-Related structure data
Related structure data |
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-Links
EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
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-Map
File | Download / File: emd_44254.map.gz / Format: CCP4 / Size: 512 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||||||||||||||||||
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Projections & slices | Image control
Images are generated by Spider. | ||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 2.05 Å | ||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Mask #1
File | emd_44254_msk_1.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Half map: #1
File | emd_44254_half_map_1.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Half map: #2
File | emd_44254_half_map_2.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Sample components
-Entire : Flagellar C-ring containing FliF, FliG, FliM, and FliN
Entire | Name: Flagellar C-ring containing FliF, FliG, FliM, and FliN |
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Components |
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-Supramolecule #1: Flagellar C-ring containing FliF, FliG, FliM, and FliN
Supramolecule | Name: Flagellar C-ring containing FliF, FliG, FliM, and FliN type: complex / ID: 1 / Parent: 0 |
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Source (natural) | Organism: Salmonella enterica subsp. enterica serovar Typhimurium (bacteria) |
Molecular weight | Theoretical: 3.5 MDa |
-Experimental details
-Structure determination
Method | cryo EM |
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Processing | single particle reconstruction |
Aggregation state | particle |
-Sample preparation
Buffer | pH: 7.5 |
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Vitrification | Cryogen name: ETHANE |
-Electron microscopy
Microscope | FEI TITAN KRIOS |
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Image recording | Film or detector model: GATAN K3 (6k x 4k) / Average electron dose: 56.323 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.0 µm / Nominal defocus min: 1.0 µm |
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |