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- EMDB-42050: Structure of eastern equine encephalitis virus VLP in complex wit... -
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Open data
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Basic information
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Title | Structure of eastern equine encephalitis virus VLP in complex with VLDLR LA1 | |||||||||
![]() | icosahedral asymmetrical unit subparticle map | |||||||||
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![]() | Alphaviruses / Receptor / VIRUS LIKE PARTICLE | |||||||||
Function / homology | ![]() reelin receptor activity / VLDL clearance / glycoprotein transport / ventral spinal cord development / very-low-density lipoprotein particle receptor activity / Reelin signalling pathway / very-low-density lipoprotein particle binding / reelin-mediated signaling pathway / low-density lipoprotein particle receptor activity / very-low-density lipoprotein particle clearance ...reelin receptor activity / VLDL clearance / glycoprotein transport / ventral spinal cord development / very-low-density lipoprotein particle receptor activity / Reelin signalling pathway / very-low-density lipoprotein particle binding / reelin-mediated signaling pathway / low-density lipoprotein particle receptor activity / very-low-density lipoprotein particle clearance / togavirin / very-low-density lipoprotein particle / positive regulation of dendrite development / T=4 icosahedral viral capsid / lipid transport / dendrite morphogenesis / cargo receptor activity / apolipoprotein binding / clathrin-coated pit / VLDLR internalisation and degradation / cholesterol metabolic process / receptor-mediated endocytosis / memory / calcium-dependent protein binding / symbiont-mediated suppression of host gene expression / nervous system development / symbiont-mediated suppression of host toll-like receptor signaling pathway / host cell cytoplasm / receptor complex / symbiont entry into host cell / lysosomal membrane / serine-type endopeptidase activity / fusion of virus membrane with host endosome membrane / calcium ion binding / host cell nucleus / virion attachment to host cell / host cell plasma membrane / structural molecule activity / virion membrane / signal transduction / proteolysis / RNA binding / membrane / plasma membrane / cytoplasm Similarity search - Function | |||||||||
Biological species | ![]() ![]() ![]() | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 3.58 Å | |||||||||
![]() | Abraham J / Yang P / Li W / Fan X / Pan J | |||||||||
Funding support | ![]()
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![]() | ![]() Title: Structural basis for VLDLR recognition by eastern equine encephalitis virus Authors: Abraham J / Yang P / Li W / Fan X / Pan J | |||||||||
History |
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Structure visualization
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Downloads & links
-EMDB archive
Map data | ![]() | |||
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Header (meta data) | ![]() | |||
Archive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 8ua4MC M: atomic model generated by this map C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
EMDB pages | ![]() ![]() |
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Related items in Molecule of the Month |
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Map
File | ![]() | ||||||||||||||||||||
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Annotation | icosahedral asymmetrical unit subparticle map | ||||||||||||||||||||
Voxel size | X=Y=Z: 1.06 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
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Sample components
+Entire : Eastern equine encephalitis virus
+Supramolecule #1: Eastern equine encephalitis virus
+Supramolecule #2: EEEV strain PE6 mature glycoprotein E1
+Supramolecule #3: EEEV strain PE6 mature glycoprotein E2
+Supramolecule #4: EEEV strain PE6 mature glycoprotein E3
+Supramolecule #5: EEEV strain PE6 mature capsid protein
+Supramolecule #6: VLDLR
+Macromolecule #1: Envelope glycoprotein E1
+Macromolecule #2: Envelope glycoprotein E2
+Macromolecule #3: Capsid protein
+Macromolecule #4: Envelope glycoprotein E3
+Macromolecule #5: Very low-density lipoprotein receptor
+Macromolecule #6: 2-acetamido-2-deoxy-beta-D-glucopyranose
+Macromolecule #7: CALCIUM ION
-Experimental details
-Structure determination
Method | cryo EM |
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![]() | single particle reconstruction |
Aggregation state | particle |
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Sample preparation
Buffer | pH: 7.2 |
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Vitrification | Cryogen name: ETHANE |
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Electron microscopy
Microscope | FEI TITAN KRIOS |
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Image recording | Film or detector model: GATAN K3 (6k x 4k) / Average electron dose: 53.0 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.0 µm / Nominal defocus min: 0.8 µm |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |
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Image processing
Startup model | Type of model: EMDB MAP EMDB ID: |
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Final reconstruction | Resolution.type: BY AUTHOR / Resolution: 3.58 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 185420 |
Initial angle assignment | Type: ANGULAR RECONSTITUTION |
Final angle assignment | Type: ANGULAR RECONSTITUTION |