+Open data
-Basic information
Entry | Database: EMDB / ID: EMD-4186 | |||||||||
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Title | Glutamine synthetase I from Mycobacterium smegmatis | |||||||||
Map data | Glutamine synthetase I from Mycobacterium smegmatis | |||||||||
Sample |
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Biological species | Mycobacterium smegmatis str. MC2 155 (bacteria) | |||||||||
Method | single particle reconstruction / negative staining / Resolution: 24.0 Å | |||||||||
Authors | Kirykowicz AM / Woodward JD | |||||||||
Citation | Journal: To Be Published Title: Glutamine synthetase I from Mycobacterium smegmatis Authors: Kirykowicz AM / Woodward JD | |||||||||
History |
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-Structure visualization
Movie |
Movie viewer |
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Structure viewer | EM map: SurfViewMolmilJmol/JSmol |
Supplemental images |
-Downloads & links
-EMDB archive
Map data | emd_4186.map.gz | 145.2 KB | EMDB map data format | |
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Header (meta data) | emd-4186-v30.xml emd-4186.xml | 8.6 KB 8.6 KB | Display Display | EMDB header |
Images | emd_4186.png | 27 KB | ||
Archive directory | http://ftp.pdbj.org/pub/emdb/structures/EMD-4186 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-4186 | HTTPS FTP |
-Validation report
Summary document | emd_4186_validation.pdf.gz | 199.8 KB | Display | EMDB validaton report |
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Full document | emd_4186_full_validation.pdf.gz | 198.9 KB | Display | |
Data in XML | emd_4186_validation.xml.gz | 5.2 KB | Display | |
Arichive directory | https://ftp.pdbj.org/pub/emdb/validation_reports/EMD-4186 ftp://ftp.pdbj.org/pub/emdb/validation_reports/EMD-4186 | HTTPS FTP |
-Related structure data
Similar structure data |
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-Links
EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
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-Map
File | Download / File: emd_4186.map.gz / Format: CCP4 / Size: 1 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Annotation | Glutamine synthetase I from Mycobacterium smegmatis | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Projections & slices | Image control
Images are generated by Spider. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 5.4 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
CCP4 map header:
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-Supplemental data
-Sample components
-Entire : Glutamine Synthetase I
Entire | Name: Glutamine Synthetase I |
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Components |
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-Supramolecule #1: Glutamine Synthetase I
Supramolecule | Name: Glutamine Synthetase I / type: complex / ID: 1 / Parent: 0 |
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Source (natural) | Organism: Mycobacterium smegmatis str. MC2 155 (bacteria) |
Molecular weight | Experimental: 648 KDa |
-Experimental details
-Structure determination
Method | negative staining |
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Processing | single particle reconstruction |
Aggregation state | particle |
-Sample preparation
Concentration | 0.3 mg/mL |
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Buffer | pH: 8 / Details: 50 mM Tris-HCl, 200 mM NaCl |
Staining | Type: NEGATIVE / Material: Uranyl Acetate / Details: Sample washed/stained with 2% UA and air-dried |
Grid | Material: COPPER / Pretreatment - Type: GLOW DISCHARGE / Pretreatment - Atmosphere: AIR |
Details | Obtained from partially purified fraction |
-Electron microscopy
Microscope | FEI TECNAI F20 |
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Image recording | Film or detector model: GATAN ULTRASCAN 4000 (4k x 4k) / Number real images: 152 / Average exposure time: 0.5 sec. / Average electron dose: 20.0 e/Å2 |
Electron beam | Acceleration voltage: 200 kV / Electron source: FIELD EMISSION GUN |
Electron optics | C2 aperture diameter: 70.0 µm / Illumination mode: OTHER / Imaging mode: BRIGHT FIELD / Cs: 1.2 mm / Nominal magnification: 50000 |
Experimental equipment | Model: Tecnai F20 / Image courtesy: FEI Company |