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Open data
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Basic information
Entry | ![]() | |||||||||
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Title | Ghanaian virus fusion glycoprotein (GhV F) | |||||||||
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![]() | GhV F / ![]() ![]() ![]() ![]() | |||||||||
Function / homology | ![]() ![]() ![]() ![]() Similarity search - Function | |||||||||
Biological species | ![]() ![]() ![]() ![]() | |||||||||
Method | ![]() ![]() | |||||||||
![]() | Park YJ / Seattle Structural Genomics Center for Infectious Disease (SSGCID) / Veesler D | |||||||||
Funding support | ![]()
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![]() | ![]() Title: Structure and design of Langya virus glycoprotein antigens. Authors: Zhaoqian Wang / Matthew McCallum / Lianying Yan / Cecily A Gibson / William Sharkey / Young-Jun Park / Ha V Dang / Moushimi Amaya / Ashley Person / Christopher C Broder / David Veesler / ![]() Abstract: Langya virus (LayV) is a recently discovered henipavirus (HNV), isolated from febrile patients in China. HNV entry into host cells is mediated by the attachment (G) and fusion (F) glycoproteins which ...Langya virus (LayV) is a recently discovered henipavirus (HNV), isolated from febrile patients in China. HNV entry into host cells is mediated by the attachment (G) and fusion (F) glycoproteins which are the main targets of neutralizing antibodies. We show here that the LayV F and G glycoproteins promote membrane fusion with human, mouse, and hamster target cells using a different, yet unknown, receptor than Nipah virus (NiV) and Hendra virus (HeV) and that NiV- and HeV-elicited monoclonal and polyclonal antibodies do not cross-react with LayV F and G. We determined cryoelectron microscopy structures of LayV F, in the prefusion and postfusion states, and of LayV G, revealing their conformational landscape and distinct antigenicity relative to NiV and HeV. We computationally designed stabilized LayV G constructs and demonstrate the generalizability of an HNV F prefusion-stabilization strategy. Our data will support the development of vaccines and therapeutics against LayV and closely related HNVs. | |||||||||
History |
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Structure visualization
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 63.3 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 17.6 KB 17.6 KB | Display Display | ![]() |
Images | ![]() | 87.9 KB | ||
Filedesc metadata | ![]() | 6.3 KB | ||
Others | ![]() ![]() ![]() | 33.8 MB 62.2 MB 62.2 MB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 8tvbMC ![]() 8tveC ![]() 8tvfC ![]() 8tvgC ![]() 8tvhC ![]() 8tviC ![]() 8vwpC M: atomic model generated by this map C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
EMDB pages | ![]() ![]() |
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Related items in Molecule of the Month |
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Map
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Voxel size | X=Y=Z: 1 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Additional map: #1
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-Half map: #2
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Density Histograms |
-Half map: #1
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Density Histograms |
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Sample components
-Entire : Prefusion Ghanian henipavirus F fused to the I53-50A trimer via a...
Entire | Name: Prefusion Ghanian henipavirus F fused to the I53-50A trimer via an intervening 16 GS linker |
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Components |
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-Supramolecule #1: Prefusion Ghanian henipavirus F fused to the I53-50A trimer via a...
Supramolecule | Name: Prefusion Ghanian henipavirus F fused to the I53-50A trimer via an intervening 16 GS linker type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1 |
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Source (natural) | Organism: ![]() |
-Macromolecule #1: Fusion glycoprotein F0,2-dehydro-3-deoxyphosphogluconate aldolase...
Macromolecule | Name: Fusion glycoprotein F0,2-dehydro-3-deoxyphosphogluconate aldolase/4-hydroxy-2-oxoglutarate aldolase type: protein_or_peptide / ID: 1 / Number of copies: 3 / Enantiomer: LEVO |
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Source (natural) | Organism: ![]() ![]() ![]() Strain: ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8 |
Molecular weight | Theoretical: 96.991531 KDa |
Recombinant expression | Organism: ![]() ![]() |
Sequence | String: MKKKTDNPTI SKRGHNHSRG IKSRALLRET DNYSNGLIVE NLVRNCHHPS KNNLNYTKTQ KRDSTIPYRV EERKGHYPKI KHLIDKSYK HIKRGKRRNG HNGNIITIIL LLILILKTQM SEGAIHYETL SKIGLIKGIT REYKVKGTPS SKDIVIKLIP N VTGLNKCT ...String: MKKKTDNPTI SKRGHNHSRG IKSRALLRET DNYSNGLIVE NLVRNCHHPS KNNLNYTKTQ KRDSTIPYRV EERKGHYPKI KHLIDKSYK HIKRGKRRNG HNGNIITIIL LLILILKTQM SEGAIHYETL SKIGLIKGIT REYKVKGTPS SKDIVIKLIP N VTGLNKCT NISMENYKEQ LDKILIPINN IIELYANSTK SAPGNARFAG VIIAGVALGV AAAAQITAGI ALHEARQNAE RI NLLKDSI SATNNAVAEL QEATGGIVNV ITGMQDYINT NLVPQIDKLQ CSQIKTALDI SLSQYYSEIL TVFGPNLQNP VTT SMSIQA ISQSFGGNID LLLNLLGYTA NDLLDLLESK SITGQITYIN LEHYFMVIRV YYPIMTTISN AYVQELIKIS FNVD GSEWV SLVPSYILIR NSYLSNIDIS ECLITKNSVI CRHDFAMPMS YTLKECLTGD TEKCPREAVV TSYVPRFAIS GGVIY ANCL STTCQCYQTG KVIAQDGSQT LMMIDNQTCS IVRIEEILIS TGKYLGSQEY NTMHVSVGNP VFTDKLDITS QISNIN QSI EQSKFYLDKS KAILDKINLN LIGMKQIEDK IEEILSKIYH IENEIARIKK LIGEAPGGIE GRGGSGSGGS GGSGSEK AA KAEEAARKME ELFKKHKIVA VLRANSVEEA IEKAVAVFAG GVHLIEITFT VPDADTVIKA LSVLKEKGAI IGAGTVTS V EQARKAVESG AEFIVSPHLD EEISQFAKEK GVFYMPGVMT PTELVKAMKL GHTILKLFPG EVVGPQFVKA MKGPFPNVK FVPTGGVNLD NVAEWFKAGV LAVGVGSALV KGTPDEVREK AKAFVEKIRG ATEGGSGGSH HHHHHGSGGG SGLNDIFEAQ KIEWHE UniProtKB: Fusion glycoprotein F0, 2-dehydro-3-deoxyphosphogluconate aldolase/4-hydroxy-2-oxoglutarate aldolase |
-Macromolecule #2: 2-acetamido-2-deoxy-beta-D-glucopyranose
Macromolecule | Name: 2-acetamido-2-deoxy-beta-D-glucopyranose / type: ligand / ID: 2 / Number of copies: 12 / Formula: NAG |
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Molecular weight | Theoretical: 221.208 Da |
Chemical component information | ![]() ChemComp-NAG: |
-Experimental details
-Structure determination
Method | ![]() |
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Aggregation state | particle |
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Sample preparation
Buffer | pH: 8 |
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Vitrification | Cryogen name: ETHANE |
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Electron microscopy
Microscope | FEI TITAN KRIOS |
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Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD![]() |
Image recording | Film or detector model: GATAN K3 (6k x 4k) / Average electron dose: 63.0 e/Å2 |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |
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Image processing
Startup model | Type of model: OTHER / Details: ab initio |
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Initial angle assignment | Type: PROJECTION MATCHING |
Final angle assignment | Type: PROJECTION MATCHING |
Final reconstruction | Applied symmetry - Point group: C3 (3 fold cyclic![]() |