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- EMDB-41517: Polyclonal immune complex of Fab binding the H2 HA from serum of ... -

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Entry
Database: EMDB / ID: EMD-41517
TitlePolyclonal immune complex of Fab binding the H2 HA from serum of subject 1-1 at week 20
Map dataNegative stain map of global refinement of particle stack used to sort polyclonal responses to H2 HA from human subject 1-1 at week 20
Sample
  • Complex: Polyclonal immune complex of Fab binding the H2 HA from serum of subject 1-1 at week 20
Keywordsinfluenza / hemagglutinin / H2 / polyclonal complex / Fab complex / viral fusion protein / VIRAL PROTEIN
Biological speciesHomo sapiens (human)
Methodsingle particle reconstruction / negative staining / Resolution: 20.0 Å
AuthorsYang YR / Han J / Richey ST / Ward AB
Funding support United States, 1 items
OrganizationGrant numberCountry
Bill & Melinda Gates FoundationINV-004923 United States
CitationJournal: Cell Rep / Year: 2024
Title: Immune memory shapes human polyclonal antibody responses to H2N2 vaccination.
Authors: Yuhe R Yang / Julianna Han / Hailee R Perrett / Sara T Richey / Alesandra J Rodriguez / Abigail M Jackson / Rebecca A Gillespie / Sarah O'Connell / Julie E Raab / Lauren Y Cominsky / Ankita ...Authors: Yuhe R Yang / Julianna Han / Hailee R Perrett / Sara T Richey / Alesandra J Rodriguez / Abigail M Jackson / Rebecca A Gillespie / Sarah O'Connell / Julie E Raab / Lauren Y Cominsky / Ankita Chopde / Masaru Kanekiyo / Katherine V Houser / Grace L Chen / Adrian B McDermott / Sarah F Andrews / Andrew B Ward /
Abstract: Influenza A virus subtype H2N2, which caused the 1957 influenza pandemic, remains a global threat. A recent phase 1 clinical trial investigating a ferritin nanoparticle vaccine displaying H2 ...Influenza A virus subtype H2N2, which caused the 1957 influenza pandemic, remains a global threat. A recent phase 1 clinical trial investigating a ferritin nanoparticle vaccine displaying H2 hemagglutinin (HA) in H2-naive and H2-exposed adults enabled us to perform comprehensive structural and biochemical characterization of immune memory on the breadth and diversity of the polyclonal serum antibody response elicited. We temporally map the epitopes targeted by serum antibodies after vaccine prime and boost, revealing that previous H2 exposure results in higher responses to the variable HA head domain. In contrast, initial responses in H2-naive participants are dominated by antibodies targeting conserved epitopes. We use cryoelectron microscopy and monoclonal B cell isolation to describe the molecular details of cross-reactive antibodies targeting conserved epitopes on the HA head, including the receptor-binding site and a new site of vulnerability deemed the medial junction. Our findings accentuate the impact of pre-existing influenza exposure on serum antibody responses post-vaccination.
History
DepositionAug 8, 2023-
Header (metadata) releaseMay 22, 2024-
Map releaseMay 22, 2024-
UpdateMay 22, 2024-
Current statusMay 22, 2024Processing site: RCSB / Status: Released

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Structure visualization

Supplemental images

emd_41517.png

Downloads & links

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Map

FileDownload / File: emd_41517.map.gz / Format: CCP4 / Size: 15.6 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
AnnotationNegative stain map of global refinement of particle stack used to sort polyclonal responses to H2 HA from human subject 1-1 at week 20
Projections & slices

Image control

Size
Brightness
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Others
AxesZ (Sec.)Y (Row.)X (Col.)
2.06 Å/pix.
x 160 pix.
= 329.6 Å		2.06 Å/pix.
x 160 pix.
= 329.6 Å		2.06 Å/pix.
x 160 pix.
= 329.6 Å

Surface

Projections

Slices (1/3)

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Images are generated by Spider.

Voxel sizeX=Y=Z: 2.06 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.0242
Minimum - Maximum-0.030868892 - 0.08509597
Average (Standard dev.)0.00026437672 (±0.0049625803)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions160160160
Spacing160160160
CellA=B=C: 329.59998 Å
α=β=γ: 90.0 °

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Supplemental data

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Additional map: Negative stain map of polyclonal Fab binding the...

Fileemd_41517_additional_1.map
AnnotationNegative stain map of polyclonal Fab binding the H2 HA top head 1 epitope from human subject 1-1 at week 20
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Additional map: Negative stain map of polyclonal Fab binding the...

Fileemd_41517_additional_2.map
AnnotationNegative stain map of polyclonal Fab binding the H2 HA RBS epitope from human subject 1-1 at week 20
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Additional map: Negative stain map of polyclonal Fab binding the...

Fileemd_41517_additional_3.map
AnnotationNegative stain map of polyclonal Fab binding the H2 HA top head 2 epitope from human subject 1-1 at week 20
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Additional map: Negative stain map of polyclonal Fab binding the...

Fileemd_41517_additional_4.map
AnnotationNegative stain map of polyclonal Fab binding the H2 HA central stem epitope from human subject 1-1 at week 20
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Half map: Negative stain map of half map B for...

Fileemd_41517_half_map_1.map
AnnotationNegative stain map of half map B for global refinement of particle stack used to sort polyclonal responses to H2 HA from human subject 1-1 at week 20
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: Negative stain map of half map A for...

Fileemd_41517_half_map_2.map
AnnotationNegative stain map of half map A for global refinement of particle stack used to sort polyclonal responses to H2 HA from human subject 1-1 at week 20
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : Polyclonal immune complex of Fab binding the H2 HA from serum of ...

EntireName: Polyclonal immune complex of Fab binding the H2 HA from serum of subject 1-1 at week 20
Components
  • Complex: Polyclonal immune complex of Fab binding the H2 HA from serum of subject 1-1 at week 20

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Supramolecule #1: Polyclonal immune complex of Fab binding the H2 HA from serum of ...

SupramoleculeName: Polyclonal immune complex of Fab binding the H2 HA from serum of subject 1-1 at week 20
type: complex / ID: 1 / Parent: 0
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 280 KDa

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Experimental details

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Structure determination

Methodnegative staining
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

Concentration0.015 mg/mL
BufferpH: 7.4
StainingType: NEGATIVE / Material: 2% w/v uranyl formate
GridModel: Homemade / Material: COPPER / Mesh: 400 / Support film - Material: CARBON / Pretreatment - Type: GLOW DISCHARGE

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Electron microscopy

MicroscopeFEI TECNAI SPIRIT
Image recordingFilm or detector model: FEI EAGLE (4k x 4k) / Average electron dose: 25.0 e/Å2
Electron beamAcceleration voltage: 200 kV / Electron source: LAB6
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: DARK FIELD / Nominal defocus max: 2.0 µm / Nominal defocus min: 2.0 µm
Sample stageSpecimen holder model: SIDE ENTRY, EUCENTRIC
Experimental equipment
Model: Tecnai Spirit / Image courtesy: FEI Company

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Image processing

Startup modelType of model: PDB ENTRY
PDB model - PDB ID:

3ku5

Final reconstructionResolution.type: BY AUTHOR / Resolution: 20.0 Å / Resolution method: FSC 0.5 CUT-OFF / Software - Name: RELION (ver. 3.0) / Number images used: 51147
Initial angle assignmentType: ANGULAR RECONSTITUTION
Final angle assignmentType: ANGULAR RECONSTITUTION
Final 3D classificationSoftware - Name: RELION (ver. 3.0)

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