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- EMDB-39882: ExoKCR1 channelrhodopsin -

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Basic information

Entry
Database: EMDB / ID: EMD-39882
TitleExoKCR1 channelrhodopsin
Map data
Sample
  • Complex: exoKCR1
    • Protein or peptide: exoKCR1
  • Ligand: POTASSIUM ION
  • Ligand: RETINAL
  • Ligand: water
KeywordsChannelrhodopsin / MEMBRANE PROTEIN
Biological speciesHyphochytrium catenoides (eukaryote)
Methodsingle particle reconstruction / cryo EM / Resolution: 2.6 Å
AuthorsZhang MF
Funding support1 items
OrganizationGrant numberCountry
Not funded
CitationJournal: Nat Commun / Year: 2024
Title: Channelrhodopsins with distinct chromophores and binding patterns.
Authors: Yuanyue Shan / Liping Zhao / Meiyu Chen / Xiao Li / Mingfeng Zhang / Duanqing Pei /
Abstract: Channelrhodopsins are popular optogenetic tools in neuroscience, but remain poorly understood mechanistically. Here we report the cryo-EM structures of channelrhodopsin-2 (ChR2) from Chlamydomonas ...Channelrhodopsins are popular optogenetic tools in neuroscience, but remain poorly understood mechanistically. Here we report the cryo-EM structures of channelrhodopsin-2 (ChR2) from Chlamydomonas reinhardtii and H. catenoides kalium channelrhodopsin (KCR1). We show that ChR2 recruits an endogenous N-retinylidene-PE-like molecule to a previously unidentified lateral retinal binding pocket, exhibiting a reduced light response in HEK293 cells. In contrast, H. catenoides kalium channelrhodopsin (KCR1) binds an endogenous retinal in its canonical retinal binding pocket under identical condition. However, exogenous ATR reduces the photocurrent magnitude of wild type KCR1 and also inhibits its leaky mutant C110T. Our results uncover diverse retinal chromophores with distinct binding patterns for channelrhodopsins in mammalian cells, which may further inspire next generation optogenetics for complex tasks such as cell fate control.
History
DepositionApr 25, 2024-
Header (metadata) releaseSep 4, 2024-
Map releaseSep 4, 2024-
UpdateOct 16, 2024-
Current statusOct 16, 2024Processing site: PDBc / Status: Released

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Structure visualization

Supplemental images

emd_39882.png

Downloads & links

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Map

FileDownload / File: emd_39882.map.gz / Format: CCP4 / Size: 1000 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
0.35 Å/pix.
x 640 pix.
= 224. Å		0.35 Å/pix.
x 640 pix.
= 224. Å		0.35 Å/pix.
x 640 pix.
= 224. Å

Surface

Projections

Slices (1/3)

Slices (1/2)

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Images are generated by Spider.

Voxel sizeX=Y=Z: 0.35 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.1
Minimum - Maximum-0.15059076 - 0.26047283
Average (Standard dev.)0.00018273923 (±0.007930806)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions640640640
Spacing640640640
CellA=B=C: 224.0 Å
α=β=γ: 90.0 °

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Supplemental data

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Half map: #2

Fileemd_39882_half_map_1.map
Projections & Slices
AxesZYX

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Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: #1

Fileemd_39882_half_map_2.map
Projections & Slices
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Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : exoKCR1

EntireName: exoKCR1
Components
  • Complex: exoKCR1
    • Protein or peptide: exoKCR1
  • Ligand: POTASSIUM ION
  • Ligand: RETINAL
  • Ligand: water

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Supramolecule #1: exoKCR1

SupramoleculeName: exoKCR1 / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1
Source (natural)Organism: Hyphochytrium catenoides (eukaryote)

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Macromolecule #1: exoKCR1

MacromoleculeName: exoKCR1 / type: protein_or_peptide / ID: 1 / Number of copies: 3 / Enantiomer: LEVO
Source (natural)Organism: Hyphochytrium catenoides (eukaryote)
Molecular weightTheoretical: 29.856984 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: PFYDSRPPEG WPKGSINDMD YPLLGSICAV CCVFVAGSGI WMLYRLDLGM GYSCKPYKSG RAPEVNSLSG IICLLCGTMY AAKSFDFFD GGGTPFSLNW YWYLDYVFTC PLLILDFAFT LDLPHKIRYF FAVFLTLWCG VAAFVTPSAY RFAYYALGCC W FTPFALSL ...String:
PFYDSRPPEG WPKGSINDMD YPLLGSICAV CCVFVAGSGI WMLYRLDLGM GYSCKPYKSG RAPEVNSLSG IICLLCGTMY AAKSFDFFD GGGTPFSLNW YWYLDYVFTC PLLILDFAFT LDLPHKIRYF FAVFLTLWCG VAAFVTPSAY RFAYYALGCC W FTPFALSL MRHVKERYLV YPPKCQRWLF WACVIFFGFW PMFPILFIFS WLGTGHISQQ AFYIIHAFLD LTCKSIFGIL MT VFRLELE EHTEVQGLPL NE

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Macromolecule #2: POTASSIUM ION

MacromoleculeName: POTASSIUM ION / type: ligand / ID: 2 / Number of copies: 15 / Formula: K
Molecular weightTheoretical: 39.098 Da

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Macromolecule #3: RETINAL

MacromoleculeName: RETINAL / type: ligand / ID: 3 / Number of copies: 3 / Formula: RET
Molecular weightTheoretical: 284.436 Da
Chemical component information

ChemComp-RET:
RETINAL

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Macromolecule #4: water

MacromoleculeName: water / type: ligand / ID: 4 / Number of copies: 30 / Formula: HOH
Molecular weightTheoretical: 18.015 Da
Chemical component information

ChemComp-HOH:
WATER

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 7.2
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Image recordingFilm or detector model: FEI FALCON IV (4k x 4k) / Average electron dose: 60.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.0 µm / Nominal defocus min: 1.2 µm
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Startup modelType of model: NONE
Final reconstructionResolution.type: BY AUTHOR / Resolution: 2.6 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 24500
Initial angle assignmentType: NOT APPLICABLE
Final angle assignmentType: NOT APPLICABLE

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