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Yorodumi- EMDB-39767: Cryo-EM structure of CTR-bound type 7 CRISPR-Cas complex at subst... -
+Open data
-Basic information
Entry | Database: EMDB / ID: EMD-39767 | |||||||||
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Title | Cryo-EM structure of CTR-bound type 7 CRISPR-Cas complex at substrate-engaged state 1 | |||||||||
Map data | ||||||||||
Sample |
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Keywords | a protein complex / ANTIVIRAL PROTEIN | |||||||||
Biological species | metagenome (others) | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 2.85 Å | |||||||||
Authors | Zhang H / Deng Z / Li X | |||||||||
Funding support | China, 1 items
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Citation | Journal: To Be Published Title: Cryo-EM structure of CTR-bound type 7 CRISPR-Cas complex at substrate-engaged state 1 Authors: Li X / Deng Z / Zhang H | |||||||||
History |
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-Structure visualization
Supplemental images |
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-Downloads & links
-EMDB archive
Map data | emd_39767.map.gz | 97.2 MB | EMDB map data format | |
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Header (meta data) | emd-39767-v30.xml emd-39767.xml | 16.5 KB 16.5 KB | Display Display | EMDB header |
FSC (resolution estimation) | emd_39767_fsc.xml | 9.9 KB | Display | FSC data file |
Images | emd_39767.png | 65.4 KB | ||
Filedesc metadata | emd-39767.cif.gz | 5.9 KB | ||
Others | emd_39767_half_map_1.map.gz emd_39767_half_map_2.map.gz | 95.7 MB 95.7 MB | ||
Archive directory | http://ftp.pdbj.org/pub/emdb/structures/EMD-39767 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-39767 | HTTPS FTP |
-Related structure data
Related structure data | 8z4l M: atomic model generated by this map |
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-Links
EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
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-Map
File | Download / File: emd_39767.map.gz / Format: CCP4 / Size: 103 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||||||||||||||||||
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Projections & slices | Image control
Images are generated by Spider. | ||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 0.95 Å | ||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Half map: #2
File | emd_39767_half_map_1.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Half map: #1
File | emd_39767_half_map_2.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Sample components
-Entire : a protein
Entire | Name: a protein |
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Components |
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-Supramolecule #1: a protein
Supramolecule | Name: a protein / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#5 |
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Source (natural) | Organism: metagenome (others) |
-Macromolecule #1: a protein
Macromolecule | Name: a protein / type: protein_or_peptide / ID: 1 / Number of copies: 7 / Enantiomer: LEVO |
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Source (natural) | Organism: metagenome (others) |
Molecular weight | Theoretical: 22.255604 KDa |
Recombinant expression | Organism: Escherichia coli (E. coli) |
Sequence | String: MAKTMKKIYV TMKTLSPLYT GEVRREDKEA AQKRVNFPVR KTATNKVLIP FKGALRSALE IMLKAKGENV CDTGESRARP CGRCVTCSL FGSMGRAGRA SVDFLISNDT KEQIVRESTH LRIERQTKSA SDTFKGEEVI EGATFTATIT ISNPQEKDLS L IQSALKFI ...String: MAKTMKKIYV TMKTLSPLYT GEVRREDKEA AQKRVNFPVR KTATNKVLIP FKGALRSALE IMLKAKGENV CDTGESRARP CGRCVTCSL FGSMGRAGRA SVDFLISNDT KEQIVRESTH LRIERQTKSA SDTFKGEEVI EGATFTATIT ISNPQEKDLS L IQSALKFI EENGIGGWLN KGYGRVSFEV KSEDVATDRF LK |
-Macromolecule #2: a protein
Macromolecule | Name: a protein / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO |
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Source (natural) | Organism: metagenome (others) |
Molecular weight | Theoretical: 27.139533 KDa |
Recombinant expression | Organism: Escherichia coli (E. coli) |
Sequence | String: MKEIKGILES ITGFSIPLDN GEYALYPAGR HLRGAIGYIA FNLDLPISSK FLDFDFDDII FRDLLPISKC GKIFYPEKNS NSLKCPSCN EIYGSSVLRN IMARGLSYKE VIEGKKYRLS IIVKDEKYLN EMEAIIRYIL SYGIYLGNKV SKGYGKFKIK E YSIVDILP ...String: MKEIKGILES ITGFSIPLDN GEYALYPAGR HLRGAIGYIA FNLDLPISSK FLDFDFDDII FRDLLPISKC GKIFYPEKNS NSLKCPSCN EIYGSSVLRN IMARGLSYKE VIEGKKYRLS IIVKDEKYLN EMEAIIRYIL SYGIYLGNKV SKGYGKFKIK E YSIVDILP VKDSEVLLLS DAIIDNGEKD IVFSKKEISS SKFEIIRKRG KAKGDIIRDN NHNGFYIGKY GGLGFGEIIS LK |
-Macromolecule #3: a protein
Macromolecule | Name: a protein / type: protein_or_peptide / ID: 3 / Number of copies: 4 / Enantiomer: LEVO |
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Source (natural) | Organism: metagenome (others) |
Molecular weight | Theoretical: 70.468898 KDa |
Recombinant expression | Organism: Escherichia coli (E. coli) |
Sequence | String: MIKFIGGASK VTGSAFLLET GNAKILIDCG IEQEKGIEKD NNEIIEKKIN EIGKADICIL THAHLAASGL VPLLVKKRKV NKIISTPAT KELCRLLFND FQRIQEENND IPLYSYDDIE SSFEIWDEID DRNTIELFDT KITFYNNSHI IGSVSVFIET H NGNYLFSG ...String: MIKFIGGASK VTGSAFLLET GNAKILIDCG IEQEKGIEKD NNEIIEKKIN EIGKADICIL THAHLAASGL VPLLVKKRKV NKIISTPAT KELCRLLFND FQRIQEENND IPLYSYDDIE SSFEIWDEID DRNTIELFDT KITFYNNSHI IGSVSVFIET H NGNYLFSG DIGSKLQQLM DYPPDMPDGN VDYLILESTY GNKSHDSSDR DRLLEIAKTT CENGGKVLIP SFAIGRLQEV LY TFSNYNF NFPVYIDSPM GSKVTNLIKE YNIYLKKKLR RLSITDDLFN NKYIAINTSN QSKELSNSKE PAVIISASGM LEG GRILNH LEQIKNDENS TLIFVGYQAQ NTRGRKILDG EEKVRCRIEK LNSFSAHADQ DELIDYIERL KYTPYKVFLV HGEK EQREI LAKRIISKKI RVELPENYSQ GKEILIEKKV VLNINTDNMC NFASYRLMPF SGFIVEKDDR IEINDKNWFD MIWNE EYNK MRSQIVAEDF STDQNEDSMA LPDMSHDKII ENIEYLFNIK ILSKNRIKEF WEEFCKGQKA AIKYITQVHR KNPNTG RRN WNPPEGDFTD NEIEKLYETA YNTLLSLIKY DKNKVYNILI NFNPKL |
-Macromolecule #4: RNA (49-MER)
Macromolecule | Name: RNA (49-MER) / type: rna / ID: 4 / Number of copies: 1 |
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Source (natural) | Organism: metagenome (others) |
Molecular weight | Theoretical: 19.142277 KDa |
Sequence | String: GGUUAAAACU CUUCUCAUGC UGGAUUCGAA AUUAGGUGCG CUUCGCGUUU AAGUCCCAUA |
-Macromolecule #5: RNA (40-MER)
Macromolecule | Name: RNA (40-MER) / type: rna / ID: 5 / Number of copies: 1 |
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Source (natural) | Organism: metagenome (others) |
Molecular weight | Theoretical: 19.354707 KDa |
Sequence | String: GAACAGAAGA ACACCUAAAC GCGAAGCGCA CCUAAUUUCG AAUCCAGCAU GAGAAGCUAA |
-Macromolecule #6: ZINC ION
Macromolecule | Name: ZINC ION / type: ligand / ID: 6 / Number of copies: 9 / Formula: ZN |
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Molecular weight | Theoretical: 65.409 Da |
-Experimental details
-Structure determination
Method | cryo EM |
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Processing | single particle reconstruction |
Aggregation state | particle |
-Sample preparation
Buffer | pH: 7.5 |
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Vitrification | Cryogen name: ETHANE |
-Electron microscopy
Microscope | JEOL CRYO ARM 300 |
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Image recording | Film or detector model: GATAN K3 (6k x 4k) / Average electron dose: 40.0 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.5 µm / Nominal defocus min: 0.5 µm |