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- EMDB-39494: Cryo-EM structure of ButCD complex -

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Basic information

Entry
Database: EMDB / ID: EMD-39494
TitleCryo-EM structure of ButCD complex
Map data
Sample
  • Complex: ButCD complex
    • Protein or peptide: Membrane protein
    • Protein or peptide: SusC/RagA family TonB-linked outer membrane protein
Keywordstransporter / TonB-dependent / MEMBRANE PROTEIN
Function / homology
Function and homology information


cell outer membrane / membrane
Similarity search - Function
TonB-dependent outer membrane protein, SusC/RagA / TonB-dependent outer membrane protein SusC/RagA, conserved site / CarboxypepD_reg-like domain / SusD-like, N-terminal / Starch-binding associating with outer membrane / RagB/SusD domain / SusD family / Carboxypeptidase-like, regulatory domain superfamily / TonB-dependent receptor (TBDR) proteins profile. / Vitamin B12 transporter BtuB-like ...TonB-dependent outer membrane protein, SusC/RagA / TonB-dependent outer membrane protein SusC/RagA, conserved site / CarboxypepD_reg-like domain / SusD-like, N-terminal / Starch-binding associating with outer membrane / RagB/SusD domain / SusD family / Carboxypeptidase-like, regulatory domain superfamily / TonB-dependent receptor (TBDR) proteins profile. / Vitamin B12 transporter BtuB-like / TonB-dependent receptor, plug domain superfamily / TonB-dependent receptor, plug domain / TonB-dependent receptor-like, beta-barrel domain superfamily / TonB-dependent Receptor Plug Domain / Prokaryotic membrane lipoprotein lipid attachment site profile. / Tetratricopeptide-like helical domain superfamily
Similarity search - Domain/homology
Membrane protein / SusC/RagA family TonB-linked outer membrane protein
Similarity search - Component
Biological speciesBacteroides fragilis (bacteria)
Methodsingle particle reconstruction / cryo EM / Resolution: 2.97 Å
AuthorsXu JH / Chen Z / Gao X
Funding support1 items
OrganizationGrant numberCountry
Not funded
CitationJournal: Nat Commun / Year: 2024
Title: A highly conserved SusCD transporter determines the import and species-specific antagonism of Bacteroides ubiquitin homologues
Authors: Xu JH / Chen Z / Gao X
History
DepositionMar 18, 2024-
Header (metadata) releaseSep 25, 2024-
Map releaseSep 25, 2024-
UpdateSep 25, 2024-
Current statusSep 25, 2024Processing site: PDBj / Status: Released

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Structure visualization

Supplemental images

emd_39494.png

Downloads & links

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Map

FileDownload / File: emd_39494.map.gz / Format: CCP4 / Size: 103 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
1.18 Å/pix.
x 300 pix.
= 354. Å		1.18 Å/pix.
x 300 pix.
= 354. Å		1.18 Å/pix.
x 300 pix.
= 354. Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 1.18 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 1.5
Minimum - Maximum-10.773016 - 14.9001255
Average (Standard dev.)0.00086447893 (±0.33211267)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions300300300
Spacing300300300
CellA=B=C: 353.99997 Å
α=β=γ: 90.0 °

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Supplemental data

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Sample components

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Entire : ButCD complex

EntireName: ButCD complex
Components
  • Complex: ButCD complex
    • Protein or peptide: Membrane protein
    • Protein or peptide: SusC/RagA family TonB-linked outer membrane protein

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Supramolecule #1: ButCD complex

SupramoleculeName: ButCD complex / type: complex / ID: 1 / Parent: 0 / Macromolecule list: all
Source (natural)Organism: Bacteroides fragilis (bacteria)

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Macromolecule #1: Membrane protein

MacromoleculeName: Membrane protein / type: protein_or_peptide / ID: 1 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Bacteroides fragilis (bacteria)
Molecular weightTheoretical: 48.007312 KDa
SequenceString: CDSYLDIRPV GSVIPQTAEE YRALLARAYL NVPNDRGLAC LRSDEMLVND NEYDRNSYGD IERWNDVSPF PGTSQFTWSN FYNVLFIAN QVIESQKEIT EGTPEVVNQL VGEAHLLRAY LHFVLVNLHG QPYTKSGALN SKSIPLKLDT DLEKTLGRNT V EEVYTSIL ...String:
CDSYLDIRPV GSVIPQTAEE YRALLARAYL NVPNDRGLAC LRSDEMLVND NEYDRNSYGD IERWNDVSPF PGTSQFTWSN FYNVLFIAN QVIESQKEIT EGTPEVVNQL VGEAHLLRAY LHFVLVNLHG QPYTKSGALN SKSIPLKLDT DLEKTLGRNT V EEVYTSIL SDIEHARELI NKEKWETVFS YRFNVLSVDA LQSRVSLYMG AWPKCLESAE AVLAKKSVLV DMNETPLALP NH FESVESI TALEQVMGSS VNNAVWVPAT FLALYQEGDK RLAAYFAAPD ENGNRKSSKG GKREFSCTFR VGELYLNAAE AAA NMDKLP HARMRLLELM RKRYTPEAYA KKENAVNVMD KNALISEILN ERARELAFEG HRWFDLRRTT RPRMVKVLQG KTYI LEQDD PRYTIPIPRD AIAANPGL

UniProtKB: Membrane protein

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Macromolecule #2: SusC/RagA family TonB-linked outer membrane protein

MacromoleculeName: SusC/RagA family TonB-linked outer membrane protein / type: protein_or_peptide / ID: 2 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Bacteroides fragilis (bacteria)
Molecular weightTheoretical: 111.314523 KDa
SequenceString: DAVVVTGYQT VERRKLTAAV GKLNISDETI GAVKSIDQAL AGQIAGLSVT STSGAPGAPA KIRIRGTSSL NGTQDPLWVL DGIPLEGTD VPQSNVLNDV SNIQQSSIAG LNPADIENIT VLKDAAATAI YGARAANGVI VITTKKGKVG KPVINFSSKF T YMPTLSTN ...String:
DAVVVTGYQT VERRKLTAAV GKLNISDETI GAVKSIDQAL AGQIAGLSVT STSGAPGAPA KIRIRGTSSL NGTQDPLWVL DGIPLEGTD VPQSNVLNDV SNIQQSSIAG LNPADIENIT VLKDAAATAI YGARAANGVI VITTKKGKVG KPVINFSSKF T YMPTLSTN RLNMLNSQEK VDLELELLRS NFAYGDNKGG VSKIISGYGL TDAYKKGGWS ALTPEAQTDI SRLRNTETDW GD ILFRDAF NQEYSLSLSG GNERVTYYTS IGYYQENGNV KGVGLDRLNI VAKTSYKVNR MLKFGVSLFV NRRNNKTYLT DTY GLVNPV YYSRKANPYY QPFDANGNYV YDFDVQNNSD TDLGFNIFEE RKNTSNEETI NALSSIFDAE LRFNDKLKFT TQLG LQLDK ASKEQIADKE SFSMRIIRKN SKYWDSASQS NKYFIPDGGV HKAYENTNSQ ITWKAMGEYR DSFNDIHELE VMVGT ELRK TWYETLFSAG YGFDRQTLTT KPVVFPDEDR ARQFPLHQKT YKENAYVSFF STASYSLMNR YTFGGSIRFD GSDLFG VDK KYRYLPLYSV SGLWRLSNEP FMQGTRKWMD NLAFRVSYGI QGNIDKNTSP FLLGKYIVDN ILPGGSEHMI DINSAPN KK LRWEKTQSVN VGLDFSVLNQ ALNLSVDYYY RKGTDLIGKQ MLPLETGFVS TNINWASMVN KGVEVSLSTR NVATKNFS W YTNLNFAYNN NKVLREAIPE AQTIPGREGY PVDAIFAIKT AGLDEEGYPL FYDKEGKKVT LKELYRLQDP FGLGFTVNS DVTPAEERSF YSYIGSQDTP YTGGLINTFS YKNWELTANL SFNLGGYVRT TPSYNFINFD RGQNVNSDIL DRWTPENTDG RLPALITSE KRADEYYWYD QKSEIYKNLD IWVKKLNYFR LQNLRLGYRL PEKMTKSLGM GSASVAIEGR NLLVFGSSYK N FLDPESMY NPYAPPIPKS ITFSLNLNF

UniProtKB: SusC/RagA family TonB-linked outer membrane protein

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 8
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Image recordingFilm or detector model: FEI FALCON IV (4k x 4k) / Average electron dose: 60.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.0 µm / Nominal defocus min: 0.5 µm
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Startup modelType of model: NONE
Final reconstructionResolution.type: BY AUTHOR / Resolution: 2.97 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 619121
Initial angle assignmentType: MAXIMUM LIKELIHOOD
Final angle assignmentType: MAXIMUM LIKELIHOOD

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