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- EMDB-38088: Cryo-EM structure of Staphylococcus aureus sigB-dependent RNAP-pr... -

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Basic information

Entry
Database: EMDB / ID: EMD-38088
TitleCryo-EM structure of Staphylococcus aureus sigB-dependent RNAP-promoter open complex
Map data
Sample
  • Complex: sigB-dependent RNAP-promoter open complex
    • Protein or peptide: DNA-directed RNA polymerase subunit alpha
    • Protein or peptide: DNA-directed RNA polymerase subunit beta
    • Protein or peptide: DNA-directed RNA polymerase subunit beta'
    • Protein or peptide: Probable DNA-directed RNA polymerase subunit delta
    • Protein or peptide: DNA-directed RNA polymerase subunit omega
    • Protein or peptide: DNA-directed RNA polymerase subunit epsilon
    • Protein or peptide: RNA polymerase sigma factor
    • DNA: DNA (70-mer)
    • DNA: DNA (70-mer)
KeywordsRNA polymerase / transcription / Staphylococcus aureus / sigB
Function / homology
Function and homology information


sigma factor activity / DNA-directed RNA polymerase complex / DNA-templated transcription initiation / ribonucleoside binding / DNA-directed 5'-3' RNA polymerase activity / DNA-directed RNA polymerase / protein dimerization activity / DNA-templated transcription / regulation of DNA-templated transcription / magnesium ion binding ...sigma factor activity / DNA-directed RNA polymerase complex / DNA-templated transcription initiation / ribonucleoside binding / DNA-directed 5'-3' RNA polymerase activity / DNA-directed RNA polymerase / protein dimerization activity / DNA-templated transcription / regulation of DNA-templated transcription / magnesium ion binding / DNA binding / zinc ion binding / cytoplasm
Similarity search - Function
RNA polymerase sigma-B factor / RNA polymerase sigma-B/F/G type / RNA polymerase epsilon subunit / RNA polymerase epsilon subunit / DNA-directed RNA polymerase subunit delta / DNA-directed RNA polymerase subunit delta, N-terminal domain superfamily / ASXL, HARE-HTH domain / HB1, ASXL, restriction endonuclease HTH domain / HARE-type HTH domain profile. / Sigma-70 factors family signature 1. ...RNA polymerase sigma-B factor / RNA polymerase sigma-B/F/G type / RNA polymerase epsilon subunit / RNA polymerase epsilon subunit / DNA-directed RNA polymerase subunit delta / DNA-directed RNA polymerase subunit delta, N-terminal domain superfamily / ASXL, HARE-HTH domain / HB1, ASXL, restriction endonuclease HTH domain / HARE-type HTH domain profile. / Sigma-70 factors family signature 1. / RNA polymerase sigma-70 region 3 / Sigma-70 region 3 / Sigma-70 factors family signature 2. / RNA polymerase sigma-70 / RNA polymerase sigma-70 region 4 / Sigma-70, region 4 / RNA polymerase sigma-70 region 2 / RNA polymerase sigma-70 like domain / Sigma-70 region 2 / RNA polymerase sigma factor, region 2 / RNA polymerase sigma factor, region 3/4-like / DNA-directed RNA polymerase, omega subunit / DNA-directed RNA polymerase, subunit beta-prime, bacterial type / DNA-directed RNA polymerase, beta subunit, external 1 domain / RNA polymerase beta subunit external 1 domain / RNA polymerase, alpha subunit, C-terminal / Bacterial RNA polymerase, alpha chain C terminal domain / DNA-directed RNA polymerase, alpha subunit / DNA-directed RNA polymerase beta subunit, bacterial-type / RNA polymerase Rpb6 / RNA polymerase, subunit omega/Rpo6/RPB6 / RNA polymerase Rpb6 / RNA polymerase Rpb1, domain 3 superfamily / RNA polymerase Rpb1, clamp domain superfamily / RPB6/omega subunit-like superfamily / DNA-directed RNA polymerase, subunit beta-prime / RNA polymerase Rpb2, domain 2 superfamily / RNA polymerase Rpb1, domain 3 / RNA polymerase Rpb1, domain 3 / RNA polymerase Rpb1, domain 1 / RNA polymerase Rpb1, domain 1 / RNA polymerase, alpha subunit / RNA polymerase Rpb1, domain 4 / RNA polymerase Rpb1, domain 2 / RNA polymerase Rpb1, domain 4 / RNA polymerase, beta subunit, protrusion / RNA polymerase beta subunit / RNA polymerase, N-terminal / RNA polymerase Rpb1, funnel domain superfamily / RNA polymerase I subunit A N-terminus / RNA polymerase Rpb1, domain 5 / RNA polymerase Rpb1, domain 5 / DNA-directed RNA polymerase, insert domain / DNA-directed RNA polymerase, RpoA/D/Rpb3-type / RNA polymerase Rpb3/RpoA insert domain / RNA polymerase Rpb3/Rpb11 dimerisation domain / RNA polymerases D / DNA-directed RNA polymerase, insert domain superfamily / RNA polymerase, RBP11-like subunit / RNA polymerase Rpb2, domain 2 / RNA polymerase Rpb2, domain 2 / RNA polymerase, beta subunit, conserved site / RNA polymerase Rpb2, domain 7 / RNA polymerase Rpb2, domain 3 / RNA polymerase Rpb2, OB-fold / RNA polymerase Rpb2, domain 7 / RNA polymerase Rpb2, domain 3 / RNA polymerases beta chain signature. / DNA-directed RNA polymerase, subunit 2, hybrid-binding domain / DNA-directed RNA polymerase, subunit 2 / DNA-directed RNA polymerase, subunit 2, hybrid-binding domain superfamily / RNA polymerase Rpb2, domain 6 / Winged helix-like DNA-binding domain superfamily
Similarity search - Domain/homology
DNA-directed RNA polymerase subunit alpha / DNA-directed RNA polymerase subunit beta' / RNA polymerase sigma factor / Probable DNA-directed RNA polymerase subunit delta / DNA-directed RNA polymerase subunit epsilon / DNA-directed RNA polymerase subunit beta / DNA-directed RNA polymerase subunit omega
Similarity search - Component
Biological speciesStaphylococcus aureus (bacteria)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.3 Å
AuthorsYuan L / Xu L / Liu Q / Feng Y
Funding support China, 1 items
OrganizationGrant numberCountry
National Natural Science Foundation of China (NSFC) China
CitationJournal: Nat Commun / Year: 2024
Title: Structural basis of promoter recognition by Staphylococcus aureus RNA polymerase.
Authors: Linggang Yuan / Qingyang Liu / Liqiao Xu / Bing Wu / Yu Feng /
Abstract: Bacterial RNAP needs to form holoenzyme with σ factors to initiate transcription. While Staphylococcus aureus σ controls housekeeping functions, S. aureus σ regulates virulence, biofilm formation, ...Bacterial RNAP needs to form holoenzyme with σ factors to initiate transcription. While Staphylococcus aureus σ controls housekeeping functions, S. aureus σ regulates virulence, biofilm formation, persistence, cell internalization, membrane transport, and antimicrobial resistance. Besides the sequence difference, the spacers between the -35 element and -10 element of σ regulated promoters are shorter than those of σ regulated promoters. Therefore, how σ recognizes and initiates transcription from target promoters can not be inferred from that of the well studied σ. Here, we report the cryo-EM structures of S. aureus RNAP-promoter open complexes comprising σ and σ, respectively. Structural analyses, in combination with biochemical experiments, reveal the structural basis for the promoter specificity of S. aureus transcription. Although the -10 element of σ regulated promoters is recognized by domain σ as single-stranded DNA, the -10 element of σ regulated promoters is co-recognized by domains σ and σ as double-stranded DNA, accounting for the short spacers of σ regulated promoters. S. aureus RNAP is a validated target of antibiotics, and our structures pave the way for rational drug design targeting S. aureus RNAP.
History
DepositionNov 21, 2023-
Header (metadata) releaseJun 5, 2024-
Map releaseJun 5, 2024-
UpdateJun 19, 2024-
Current statusJun 19, 2024Processing site: PDBj / Status: Released

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Structure visualization

Supplemental images

emd_38088.png

Downloads & links

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Map

FileDownload / File: emd_38088.map.gz / Format: CCP4 / Size: 40.6 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Voxel sizeX=Y=Z: 1.19 Å
Density
Contour LevelBy AUTHOR: 0.01
Minimum - Maximum-0.06307855 - 0.10763293
Average (Standard dev.)0.00019492378 (±0.0044690245)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions220220220
Spacing220220220
CellA=B=C: 261.80002 Å
α=β=γ: 90.0 °

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Supplemental data

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Half map: #2

Fileemd_38088_half_map_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: #1

Fileemd_38088_half_map_2.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : sigB-dependent RNAP-promoter open complex

EntireName: sigB-dependent RNAP-promoter open complex
Components
  • Complex: sigB-dependent RNAP-promoter open complex
    • Protein or peptide: DNA-directed RNA polymerase subunit alpha
    • Protein or peptide: DNA-directed RNA polymerase subunit beta
    • Protein or peptide: DNA-directed RNA polymerase subunit beta'
    • Protein or peptide: Probable DNA-directed RNA polymerase subunit delta
    • Protein or peptide: DNA-directed RNA polymerase subunit omega
    • Protein or peptide: DNA-directed RNA polymerase subunit epsilon
    • Protein or peptide: RNA polymerase sigma factor
    • DNA: DNA (70-mer)
    • DNA: DNA (70-mer)

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Supramolecule #1: sigB-dependent RNAP-promoter open complex

SupramoleculeName: sigB-dependent RNAP-promoter open complex / type: complex / ID: 1 / Parent: 0 / Macromolecule list: all
Source (natural)Organism: Staphylococcus aureus (bacteria)

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Macromolecule #1: DNA-directed RNA polymerase subunit alpha

MacromoleculeName: DNA-directed RNA polymerase subunit alpha / type: protein_or_peptide / ID: 1 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 35.052656 KDa
Recombinant expressionOrganism: Escherichia coli (E. coli)
SequenceString: MIEIEKPRIE TIEISEDAKF GKFVVEPLER GYGTTLGNSL RRILLSSLPG AAVKYIEIEG VLHEFSAVDN VVEDVSTIIM NIKQLALKI YSEEDKTLEI DVRDEGEVTA SDITHDSDVE ILNPELKIAT VSKGGHLKIR LVANKGRGYA LAEQNNTSDL P IGVIPVDS ...String:
MIEIEKPRIE TIEISEDAKF GKFVVEPLER GYGTTLGNSL RRILLSSLPG AAVKYIEIEG VLHEFSAVDN VVEDVSTIIM NIKQLALKI YSEEDKTLEI DVRDEGEVTA SDITHDSDVE ILNPELKIAT VSKGGHLKIR LVANKGRGYA LAEQNNTSDL P IGVIPVDS LYSPVERVNY TVENTRVGQS SDFDKLTLDV WTNGSITPQE SVSLAAKIMT EHLNIFVGLT DEAQNAEIMI EK EEDQKEK VLEMSIEELD LSVRSYNCLK RAGINSVQEL ADKSEADMMK VRNLGRKSLE EVKYKLEDLG LGLRKED

UniProtKB: DNA-directed RNA polymerase subunit alpha

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Macromolecule #2: DNA-directed RNA polymerase subunit beta

MacromoleculeName: DNA-directed RNA polymerase subunit beta / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 133.381312 KDa
Recombinant expressionOrganism: Escherichia coli (E. coli)
SequenceString: MAGQVVQYGR HRKRRNYARI SEVLELPNLI EIQTKSYEWF LREGLIEMFR DISPIEDFTG NLSLEFVDYR LGEPKYDLEE SKNRDATYA APLRVKVRLI IKETGEVKEQ EVFMGDFPLM TDTGTFVING AERVIVSQLV RSPSVYFNEK IDKNGRENYD A TIIPNRGA ...String:
MAGQVVQYGR HRKRRNYARI SEVLELPNLI EIQTKSYEWF LREGLIEMFR DISPIEDFTG NLSLEFVDYR LGEPKYDLEE SKNRDATYA APLRVKVRLI IKETGEVKEQ EVFMGDFPLM TDTGTFVING AERVIVSQLV RSPSVYFNEK IDKNGRENYD A TIIPNRGA WLEYETDAKD VVYVRIDRTR KLPLTVLLRA LGFSSDQEIV DLLGDNEYLR NTLEKDGTEN TEQALLEIYE RL RPGEPPT VENAKSLLYS RFFDPKRYDL ASVGRYKTNK KLHLKHRLFN QKLAEPIVNT ETGEIVVEEG TVLDRRKIDE IMD VLESNA NSEVFELHGS VIDEPVEIQS IKVYVPNDDE GRTTTVIGNA FPDSEVKCIT PADIIASMSY FFNLLSGIGY TDDI DHLGN RRLRSVGELL QNQFRIGLSR MERVVRERMS IQDTESITPQ QLINIRPVIA SIKEFFGSSQ LSQFMDQANP LAELT HKRR LSALGPGGLT RERAQMEVRD VHYSHYGRMC PIETPEGPNI GLINSLSSYA RVNEFGFIET PYRKVDLDTH AITDQI DYL TADEEDSYVV AQANSKLDEN GRFMDDEVVC RFRGNNTVMA KEKMDYMDVS PKQVVSAATA CIPFLENDDS NRALMGA NM QRQAVPLMNP EAPFVGTGME HVAARDSGAA ITAKHRGRVE HVESNEILVR RLVEENGVEH EGELDRYPLA KFKRSNSG T CYNQRPIVAV GDVVEYNEIL ADGPSMELGE MALGRNVVVG FMTWDGYNYE DAVIMSERLV KDDVYTSIHI EEYESEARD TKLGPEEITR DIPNVSESAL KNLDDRGIVY IGAEVKDGDI LVGKVTPKGV TELTAEERLL HAIFGEKARE VRDTSLRVPH GAGGIVLDV KVFNREEGDD TLSPGVNQLV RVYIVQKRKI HVGDKMCGRH GNKGVISKIV PEEDMPYLPD GRPIDIMLNP L GVPSRMNI GQVLELHLGM AAKNLGIHVA SPVFDGANDD DVWSTIEEAG MARDGKTVLY DGRTGEPFDN RISVGVMYML KL AHMVDDK LHARSTGPYS LVTQQPLGGK AQFGGQRFGE MEVWALEAYG AAYTLQEILT YKSDDTVGRV KTYEAIVKGE NIS RPSVPE SFRVLMKELQ SLGLDVKVMD EQDNEIEMTD VDDDDVVERK VDLQQNDAPE TQKEVTD

UniProtKB: DNA-directed RNA polymerase subunit beta

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Macromolecule #3: DNA-directed RNA polymerase subunit beta'

MacromoleculeName: DNA-directed RNA polymerase subunit beta' / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 135.594266 KDa
Recombinant expressionOrganism: Escherichia coli (E. coli)
SequenceString: MIDVNNFHYM KIGLASPEKI RSWSFGEVKK PETINYRTLK PEKDGLFCER IFGPTKDWEC SCGKYKRVRY KGMVCDRCGV EVTKSKVRR ERMGHIELAA PVSHIWYFKG IPSRMGLLLD MSPRALEEVI YFASYVVVDP GPTGLEKKTL LSEAEFRDYY D KYPGQFVA ...String:
MIDVNNFHYM KIGLASPEKI RSWSFGEVKK PETINYRTLK PEKDGLFCER IFGPTKDWEC SCGKYKRVRY KGMVCDRCGV EVTKSKVRR ERMGHIELAA PVSHIWYFKG IPSRMGLLLD MSPRALEEVI YFASYVVVDP GPTGLEKKTL LSEAEFRDYY D KYPGQFVA KMGAEGIKDL LEEIDLDEEL KLLRDELESA TGQRLTRAIK RLEVVESFRN SGNKPSWMIL DVLPIIPPEI RP MVQLDGG RFATSDLNDL YRRVINRNNR LKRLLDLGAP GIIVQNEKRM LQEAVDALID NGRRGRPVTG PGNRPLKSLS HML KGKQGR FRQNLLGKRV DYSGRSVIAV GPSLKMYQCG LPKEMALELF KPFVMKELVQ REIATNIKNA KSKIERMDDE VWDV LEEVI REHPVLLNRA PTLHRLGIQA FEPTLVEGRA IRLHPLVTTA YNADFDGDQM AVHVPLSKEA QAEARMLMLA AQNIL NPKD GKPVVTPSQD MVLGNYYLTL ERKDAVNTGA IFNNTNEVLK AYANGFVHLH TRIGVHASSF NNPTFTEEQN KKILAT SVG KIIFNEIIPD SFAYINEPTQ ENLERKTPNR YFIDPTTLGE GGLKEYFENE ELIEPFNKKF LGNIIAEVFN RFSITDT SM MLDRMKDLGF KFSSKAGITV GVADIVVLPD KQQILDEHEK LVDRITKQFN RGLITEEERY NAVVEIWTDA KDQIQGEL M QSLDKTNPIF MMSDSGARGN ASNFTQLAGM RGLMAAPSGK IIELPITSSF REGLTVLEYF ISTHGARKGL ADTALKTAD SGYLTRRLVD VAQDVIVREE DCGTDRGLLV SDIKEGTEMI EPFIERIEGR YSKETIRHPE TDEIIIRPDE LITPEIAKKI TDAGIEQMY IRSAFTCNAR HGVCEKCYGK NLATGEKVEV GEAVGTIAAQ SIGEPGTQLT MRTFHTGGVA GSDITQGLPR I QEIFEARN PKGQAVITEI EGVVEDIKLA KDRQQEIVVK GANETRSYLA SGTSRIIVEI GQPVQRGEVL TEGSIEPKNY LS VAGLNAT ESYLLKEVQK VYRMQGVEID DKHVEVMVRQ MLRKVRIIEA GDTKLLPGSL VDIHNFTDAN REAFKHRKRP ATA KPVLLG ITKASLETES FLSAASFQET TRVLTDAAIK GKRDDLLGLK ENVIIGKLIP AGTGMRRYSD VKYEKTAKPV AEVE SQTEV TE

UniProtKB: DNA-directed RNA polymerase subunit beta'

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Macromolecule #4: Probable DNA-directed RNA polymerase subunit delta

MacromoleculeName: Probable DNA-directed RNA polymerase subunit delta / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 20.89301 KDa
Recombinant expressionOrganism: Escherichia coli (E. coli)
SequenceString:
MKIQDYTKQM VDEKSFIDMA YTLLNDKGET MNLYDIIDEF RALGDYEYEE IENRVVQFYT DLNTDGRFLN VGENLWGLRD WYSVDDIEE KIAPTIQKFD ILDADDEEDQ NLKLLGEDEM DDDDDIPAQT DDQEELNDPE DEQVEEEINH SDIVIEEDED E LDEDEEVF EDEEDFND

UniProtKB: Probable DNA-directed RNA polymerase subunit delta

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Macromolecule #5: DNA-directed RNA polymerase subunit omega

MacromoleculeName: DNA-directed RNA polymerase subunit omega / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 8.161236 KDa
Recombinant expressionOrganism: Escherichia coli (E. coli)
SequenceString:
MLNPPLNQLT SQIKSKYLIA TTAAKRAREI DEQPETELLS EYHSFKPVGR ALEEIADGKI RPVISSDYYG KE

UniProtKB: DNA-directed RNA polymerase subunit omega

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Macromolecule #6: DNA-directed RNA polymerase subunit epsilon

MacromoleculeName: DNA-directed RNA polymerase subunit epsilon / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 8.764739 KDa
Recombinant expressionOrganism: Escherichia coli (E. coli)
SequenceString:
MAVFKVFYQH NRDEVIVREN TQSLYVEAQT EEQVRRYLKD RNFNIEFITK LEGAHLDYEK ENSEHFNVEI AK

UniProtKB: DNA-directed RNA polymerase subunit epsilon

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Macromolecule #7: RNA polymerase sigma factor

MacromoleculeName: RNA polymerase sigma factor / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 29.474559 KDa
Recombinant expressionOrganism: Escherichia coli (E. coli)
SequenceString: MAKESKSANE VSPEQINQWI KEHQENKNTD AQDKLVKHYQ KLIESLAYKY SKGQSHHEDL VQVGMVGLIG AINRFDMSFE RKFEAFLVP TVIGEIKRYL RDKTWSVHVP RRIKEIGPRI KKVSDELTAE LERSPSISEI ANRLEVSEEE VLEAMEMGQS Y NALSVDHS ...String:
MAKESKSANE VSPEQINQWI KEHQENKNTD AQDKLVKHYQ KLIESLAYKY SKGQSHHEDL VQVGMVGLIG AINRFDMSFE RKFEAFLVP TVIGEIKRYL RDKTWSVHVP RRIKEIGPRI KKVSDELTAE LERSPSISEI ANRLEVSEEE VLEAMEMGQS Y NALSVDHS IEADKDGSTV TLLDIMGQQD DHYDLTEKRM ILEKILPILS DREREIIQCT FIEGLSQKET GERIGLSQMH VS RLQRTAI KKLQEAAHK

UniProtKB: RNA polymerase sigma factor

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Macromolecule #8: DNA (70-mer)

MacromoleculeName: DNA (70-mer) / type: dna / ID: 8 / Number of copies: 1 / Classification: DNA
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 21.360715 KDa
SequenceString: (DC)(DT)(DT)(DC)(DT)(DT)(DT)(DT)(DG)(DA) (DT)(DT)(DC)(DT)(DT)(DG)(DT)(DT)(DC)(DA) (DT)(DG)(DA)(DC)(DT)(DA)(DA)(DC)(DC) (DC)(DG)(DT)(DT)(DT)(DT)(DA)(DC)(DA)(DT) (DG) (DG)(DG)(DC)(DT)(DT)(DT) ...String:
(DC)(DT)(DT)(DC)(DT)(DT)(DT)(DT)(DG)(DA) (DT)(DT)(DC)(DT)(DT)(DG)(DT)(DT)(DC)(DA) (DT)(DG)(DA)(DC)(DT)(DA)(DA)(DC)(DC) (DC)(DG)(DT)(DT)(DT)(DT)(DA)(DC)(DA)(DT) (DG) (DG)(DG)(DC)(DT)(DT)(DT)(DA)(DA) (DA)(DC)(DT)(DC)(DA)(DT)(DT)(DC)(DA)(DT) (DT)(DA) (DA)(DA)(DT)(DT)(DC)(DA)(DT) (DA)(DA)(DC)

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Macromolecule #9: DNA (70-mer)

MacromoleculeName: DNA (70-mer) / type: dna / ID: 9 / Number of copies: 1 / Classification: DNA
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 21.771047 KDa
SequenceString: (DG)(DT)(DT)(DA)(DT)(DG)(DA)(DA)(DT)(DT) (DT)(DA)(DA)(DT)(DG)(DA)(DA)(DT)(DG)(DA) (DG)(DT)(DT)(DT)(DA)(DA)(DA)(DG)(DC) (DC)(DC)(DA)(DT)(DG)(DT)(DA)(DA)(DA)(DA) (DC) (DG)(DG)(DG)(DT)(DA)(DT) ...String:
(DG)(DT)(DT)(DA)(DT)(DG)(DA)(DA)(DT)(DT) (DT)(DA)(DA)(DT)(DG)(DA)(DA)(DT)(DG)(DA) (DG)(DT)(DT)(DT)(DA)(DA)(DA)(DG)(DC) (DC)(DC)(DA)(DT)(DG)(DT)(DA)(DA)(DA)(DA) (DC) (DG)(DG)(DG)(DT)(DA)(DT)(DC)(DA) (DG)(DT)(DA)(DC)(DT)(DT)(DG)(DT)(DA)(DG) (DA)(DA) (DT)(DC)(DA)(DA)(DA)(DA)(DG) (DA)(DA)(DG)

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 7.5
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Image recordingFilm or detector model: FEI FALCON IV (4k x 4k) / Average electron dose: 51.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.0 µm / Nominal defocus min: 1.0 µm
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Startup modelType of model: EMDB MAP
EMDB ID:

8585

Final reconstructionResolution.type: BY AUTHOR / Resolution: 3.3 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 95089
Initial angle assignmentType: MAXIMUM LIKELIHOOD
Final angle assignmentType: MAXIMUM LIKELIHOOD

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