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- EMDB-37388: The cryo-EM structure of the Nicotiana tabacum PEP-PAP-TEC2 -

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Basic information

Entry
Database: EMDB / ID: EMD-37388
TitleThe cryo-EM structure of the Nicotiana tabacum PEP-PAP-TEC2
Map data
Sample
  • Complex: Nicotiana tabacum PEP-PAP-TEC2
    • Protein or peptide: x 18 types
    • DNA: x 2 types
    • RNA: x 1 types
  • Ligand: x 3 types
KeywordsTRANSCRIPTION / PEP / PAP
Function / homology
Function and homology information


plastid-encoded plastid RNA polymerase complex / etioplast organization / plastid transcription / positive regulation of red or far-red light signaling pathway / plastid organization / chloroplast nucleoid / chloroplast organization / acid-amino acid ligase activity / disulfide oxidoreductase activity / plastid ...plastid-encoded plastid RNA polymerase complex / etioplast organization / plastid transcription / positive regulation of red or far-red light signaling pathway / plastid organization / chloroplast nucleoid / chloroplast organization / acid-amino acid ligase activity / disulfide oxidoreductase activity / plastid / biosynthetic process / superoxide dismutase / superoxide dismutase activity / protein-disulfide reductase activity / response to light stimulus / DNA-directed RNA polymerase complex / cell redox homeostasis / chloroplast / methyltransferase activity / ribonucleoside binding / DNA-directed 5'-3' RNA polymerase activity / DNA-directed RNA polymerase / kinase activity / regulation of cell shape / methylation / protein dimerization activity / cell division / DNA-templated transcription / positive regulation of DNA-templated transcription / magnesium ion binding / DNA binding / RNA binding / zinc ion binding / ATP binding / nucleus / metal ion binding / cytoplasm
Similarity search - Function
Protein PLASTID TRANSCRIPTIONALLY ACTIVE 12 / Protein plastid transcriptionally active 7 / PLASTID TRANSCRIPTIONALLY ACTIVE protein 6 / Protein PLASTID TRANSCRIPTIONALLY ACTIVE 10 / Thioredoxin-like protein CITRX, chloroplastic / : / (S)-ureidoglycine aminohydrolase, cupin domain / EutQ-like cupin domain / PPR repeat / Pentacotripeptide-repeat region of PRORP ...Protein PLASTID TRANSCRIPTIONALLY ACTIVE 12 / Protein plastid transcriptionally active 7 / PLASTID TRANSCRIPTIONALLY ACTIVE protein 6 / Protein PLASTID TRANSCRIPTIONALLY ACTIVE 10 / Thioredoxin-like protein CITRX, chloroplastic / : / (S)-ureidoglycine aminohydrolase, cupin domain / EutQ-like cupin domain / PPR repeat / Pentacotripeptide-repeat region of PRORP / Pentacotripeptide-repeat region of PRORP / DNA-directed RNA polymerase subunit RpoC1 / DNA-directed RNA polymerase, subunit beta'' / Smr domain / Smr domain profile. / UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate ligase / Rubisco LSMT, substrate-binding domain / Rubisco LSMT, substrate-binding domain superfamily / : / Rubisco LSMT substrate-binding / PPR repeat family / MurE/MurF, N-terminal / : / Mur ligase, N-terminal catalytic domain / Mur ligase family, catalytic domain / pfkB family of carbohydrate kinases signature 1. / Mur ligase, C-terminal / Mur ligase, C-terminal domain superfamily / Mur ligase, glutamate ligase domain / Mur ligase, central / Mur-like, catalytic domain superfamily / Mur ligase middle domain / Pentatricopeptide repeat domain / Carbohydrate/purine kinase, PfkB, conserved site / Pentatricopeptide (PPR) repeat profile. / Manganese/iron superoxide dismutase, binding site / Manganese and iron superoxide dismutases signature. / Manganese/iron superoxide dismutase / Pentatricopeptide repeat / Manganese/iron superoxide dismutase, N-terminal / Iron/manganese superoxide dismutases, alpha-hairpin domain / Manganese/iron superoxide dismutase, C-terminal / Manganese/iron superoxide dismutase, C-terminal domain superfamily / Manganese/iron superoxide dismutase, N-terminal domain superfamily / Iron/manganese superoxide dismutases, C-terminal domain / Carbohydrate kinase PfkB / pfkB family carbohydrate kinase / Ribokinase-like / Thioredoxin / SET domain / SET domain superfamily / SET domain profile. / SET domain / S1 domain profile. / RmlC-like cupin domain superfamily / DNA-directed RNA polymerase, beta subunit, external 1 domain superfamily / RNA polymerase, alpha subunit, C-terminal / Bacterial RNA polymerase, alpha chain C terminal domain / DNA-directed RNA polymerase, alpha subunit / DNA-directed RNA polymerase beta subunit, bacterial-type / S1 domain / Thioredoxin domain profile. / Thioredoxin domain / RmlC-like jelly roll fold / RNA polymerase Rpb1, domain 3 superfamily / RNA polymerase Rpb1, clamp domain superfamily / RNA polymerase Rpb2, domain 2 superfamily / DNA-directed RNA polymerase, subunit beta-prime / RNA polymerase Rpb1, domain 1 / RNA polymerase Rpb1, domain 1 / DNA-directed RNA polymerase, insert domain / DNA-directed RNA polymerase, RpoA/D/Rpb3-type / RNA polymerase Rpb3/RpoA insert domain / RNA polymerase Rpb3/Rpb11 dimerisation domain / RNA polymerases D / RNA polymerase, alpha subunit / RNA polymerase Rpb1, domain 5 / RNA polymerase Rpb1, domain 4 / RNA polymerase Rpb1, domain 2 / RNA polymerase Rpb1, domain 5 / RNA polymerase Rpb1, domain 4 / RNA polymerase, N-terminal / RNA polymerase Rpb1, funnel domain superfamily / RNA polymerase I subunit A N-terminus / DNA-directed RNA polymerase, insert domain superfamily / RNA polymerase, RBP11-like subunit / RNA polymerase Rpb2, domain 2 / RNA polymerase Rpb2, domain 2 / RNA polymerase, beta subunit, conserved site / RNA polymerase Rpb2, domain 7 / RNA polymerase Rpb2, domain 3 / RNA polymerase Rpb2, OB-fold / RNA polymerase Rpb2, domain 7 / RNA polymerase Rpb2, domain 3 / RNA polymerases beta chain signature. / DNA-directed RNA polymerase, subunit 2, hybrid-binding domain / DNA-directed RNA polymerase, subunit 2 / DNA-directed RNA polymerase, subunit 2, hybrid-binding domain superfamily / RNA polymerase Rpb2, domain 6 / Tetratricopeptide-like helical domain superfamily
Similarity search - Domain/homology
DNA-directed RNA polymerase subunit gamma / Uncharacterized protein LOC107760033 / Protein PLASTID TRANSCRIPTIONALLY ACTIVE 7-like isoform X1 / Thioredoxin-like protein CITRX1, chloroplastic / Protein PLASTID TRANSCRIPTIONALLY ACTIVE 12-like / Uncharacterized protein LOC107779769 / Protein PLASTID TRANSCRIPTIONALLY ACTIVE 10-like / Pentatricopeptide repeat-containing protein At1g74850, chloroplastic-like / Fructokinase-like 2, chloroplastic / Fructokinase-like 1, chloroplastic ...DNA-directed RNA polymerase subunit gamma / Uncharacterized protein LOC107760033 / Protein PLASTID TRANSCRIPTIONALLY ACTIVE 7-like isoform X1 / Thioredoxin-like protein CITRX1, chloroplastic / Protein PLASTID TRANSCRIPTIONALLY ACTIVE 12-like / Uncharacterized protein LOC107779769 / Protein PLASTID TRANSCRIPTIONALLY ACTIVE 10-like / Pentatricopeptide repeat-containing protein At1g74850, chloroplastic-like / Fructokinase-like 2, chloroplastic / Fructokinase-like 1, chloroplastic / superoxide dismutase / superoxide dismutase / UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2, 6-diaminopimelate ligase-like / Protein PLASTID TRANSCRIPTIONALLY ACTIVE 14-like isoform X2 / DNA-directed RNA polymerase subunit alpha / DNA-directed RNA polymerase subunit beta / DNA-directed RNA polymerase subunit beta''
Similarity search - Component
Biological speciesNicotiana tabacum (common tobacco) / synthetic construct (others)
Methodsingle particle reconstruction / cryo EM / Resolution: 2.8 Å
AuthorsWu XX / Zhang Y
Funding support China, 1 items
OrganizationGrant numberCountry
Other governmentJCYJ-SHFY-2022-012 China
CitationJournal: Cell / Year: 2024
Title: Cryo-EM structures of the plant plastid-encoded RNA polymerase.
Authors: Xiao-Xian Wu / Wen-Hui Mu / Fan Li / Shu-Yi Sun / Chao-Jun Cui / Chanhong Kim / Fei Zhou / Yu Zhang /
Abstract: Chloroplasts are green plastids in the cytoplasm of eukaryotic algae and plants responsible for photosynthesis. The plastid-encoded RNA polymerase (PEP) plays an essential role during chloroplast ...Chloroplasts are green plastids in the cytoplasm of eukaryotic algae and plants responsible for photosynthesis. The plastid-encoded RNA polymerase (PEP) plays an essential role during chloroplast biogenesis from proplastids and functions as the predominant RNA polymerase in mature chloroplasts. The PEP-centered transcription apparatus comprises a bacterial-origin PEP core and more than a dozen eukaryotic-origin PEP-associated proteins (PAPs) encoded in the nucleus. Here, we determined the cryo-EM structures of Nicotiana tabacum (tobacco) PEP-PAP apoenzyme and PEP-PAP transcription elongation complexes at near-atomic resolutions. Our data show the PEP core adopts a typical fold as bacterial RNAP. Fifteen PAPs bind at the periphery of the PEP core, facilitate assembling the PEP-PAP supercomplex, protect the complex from oxidation damage, and likely couple gene transcription with RNA processing. Our results report the high-resolution architecture of the chloroplast transcription apparatus and provide the structural basis for the mechanistic and functional study of transcription regulation in chloroplasts.
History
DepositionSep 6, 2023-
Header (metadata) releaseMar 13, 2024-
Map releaseMar 13, 2024-
UpdateOct 30, 2024-
Current statusOct 30, 2024Processing site: PDBj / Status: Released

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Structure visualization

Supplemental images

emd_37388.png

Downloads & links

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Map

FileDownload / File: emd_37388.map.gz / Format: CCP4 / Size: 669.9 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

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AxesZ (Sec.)Y (Row.)X (Col.)
0.83 Å/pix.
x 560 pix.
= 464.8 Å		0.83 Å/pix.
x 560 pix.
= 464.8 Å		0.83 Å/pix.
x 560 pix.
= 464.8 Å

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Voxel sizeX=Y=Z: 0.83 Å
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Contour LevelBy AUTHOR: 0.1
Minimum - Maximum-0.37960824 - 1.362376
Average (Standard dev.)-0.0002392651 (±0.046108633)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions560560560
Spacing560560560
CellA=B=C: 464.8 Å
α=β=γ: 90.0 °

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Supplemental data

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Mask #1

Fileemd_37388_msk_1.map
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Additional map: #2

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Additional map: #1

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Half map: #2

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Sample components

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Entire : Nicotiana tabacum PEP-PAP-TEC2

EntireName: Nicotiana tabacum PEP-PAP-TEC2
Components
  • Complex: Nicotiana tabacum PEP-PAP-TEC2
    • Protein or peptide: DNA-directed RNA polymerase subunit alpha
    • Protein or peptide: DNA-directed RNA polymerase subunit beta
    • Protein or peptide: DNA-directed RNA polymerase subunit gamma
    • Protein or peptide: DNA-directed RNA polymerase subunit beta''
    • Protein or peptide: PAP1(pTAC3)
    • Protein or peptide: Pentatricopeptide repeat-containing protein At1g74850, chloroplastic-like
    • Protein or peptide: Protein PLASTID TRANSCRIPTIONALLY ACTIVE 10-like
    • Protein or peptide: superoxide dismutase
    • Protein or peptide: Protein PLASTID TRANSCRIPTIONALLY ACTIVE 12-like
    • Protein or peptide: Fructokinase-like 1, chloroplastic
    • Protein or peptide: Fructokinase-like 2, chloroplastic
    • Protein or peptide: Protein PLASTID TRANSCRIPTIONALLY ACTIVE 14-like isoform X2
    • Protein or peptide: PAP8(pTAC6)
    • Protein or peptide: superoxide dismutase
    • Protein or peptide: Thioredoxin-like protein CITRX1, chloroplastic
    • Protein or peptide: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2, 6-diaminopimelate ligase-like
    • Protein or peptide: Protein PLASTID TRANSCRIPTIONALLY ACTIVE 7-like isoform X1
    • Protein or peptide: PAP13(pTAC18)
    • DNA: DNA (24-mer)
    • DNA: DNA (24-mer)
    • RNA: RNA (27-mer)
  • Ligand: ZINC ION
  • Ligand: MAGNESIUM ION
  • Ligand: FE (III) ION

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Supramolecule #1: Nicotiana tabacum PEP-PAP-TEC2

SupramoleculeName: Nicotiana tabacum PEP-PAP-TEC2 / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#3, #20, #4-#9, #21, #10-#19
Source (natural)Organism: Nicotiana tabacum (common tobacco)

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Macromolecule #1: DNA-directed RNA polymerase subunit alpha

MacromoleculeName: DNA-directed RNA polymerase subunit alpha / type: protein_or_peptide / ID: 1 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Nicotiana tabacum (common tobacco)
Molecular weightTheoretical: 38.663543 KDa
SequenceString: MVREKVTVST RTLQWKCVES RTDSKRLYYG RFILSPLMKG QADTIGIAMR RALLGEIEGT CITRVKSEKV PHEYSTITGI QESVHEILM NLKEIILRSN LYGTSDASIC VKGPGSVTAQ DIILPPYVEI VDNTQHIASL TEPIDFCIGL QIERNRGYLI K TPHNFQDG ...String:
MVREKVTVST RTLQWKCVES RTDSKRLYYG RFILSPLMKG QADTIGIAMR RALLGEIEGT CITRVKSEKV PHEYSTITGI QESVHEILM NLKEIILRSN LYGTSDASIC VKGPGSVTAQ DIILPPYVEI VDNTQHIASL TEPIDFCIGL QIERNRGYLI K TPHNFQDG SYPIDAVFMP VRNANHSIHS YGNGNEKQEI LFLEIWTNGS LTPKEALHEA SRNLIDLFIP FLHMEEDNLY LQ DNQHTVP LSPFTFHDKL AKLIKNKKKI ALKSIFIDQS ELPSRIYNCL KMSNIYTLLD LLNNSQEDLM KIEHFRSEDV KRI LGILEK YFVIDLAKNK F

UniProtKB: DNA-directed RNA polymerase subunit alpha

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Macromolecule #2: DNA-directed RNA polymerase subunit beta

MacromoleculeName: DNA-directed RNA polymerase subunit beta / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Nicotiana tabacum (common tobacco)
Molecular weightTheoretical: 120.698125 KDa
SequenceString: MLGDGNEGIS TIPGFNQIQF EGFCRFIDQG LTEELYKFPK IEDTDQEIEF QLFVETYQLV EPLIKERDAV YESLTYSSEL YVSAGLIWK NSRDMQEQTI FIGNIPLMNS LGTSIVNGIY RIVINQILQS PGIYYRSELD HNGISVYTGT IISDWGGRSE L EIDRKARI ...String:
MLGDGNEGIS TIPGFNQIQF EGFCRFIDQG LTEELYKFPK IEDTDQEIEF QLFVETYQLV EPLIKERDAV YESLTYSSEL YVSAGLIWK NSRDMQEQTI FIGNIPLMNS LGTSIVNGIY RIVINQILQS PGIYYRSELD HNGISVYTGT IISDWGGRSE L EIDRKARI WARVSRKQKI SILVLSSAMG LNLREILENV CYPEIFLSFL SDKERKKIGS KENAILEFYQ QFACVGGDPV FS ESLCKEL QKKFFQQRCE LGRIGRRNMN RRLNLDIPQN NTFLLPRDIL AAADHLIGLK FGMGALDDMN HLKNKRIRSV ADL LQDQFG LALVRLENVV RGTICGAIRH KLIPTPQNLV TSTPLTTTYE SFFGLHPLSQ VLDRTNPLTQ IVHGRKLSYL GPGG LTGRT ASFRIRDIHP SHYGRICPID TSEGINVGLI GSLAIHARIG HWGSLESPFY EISERSTGVR MLYLSPGRDE YYMVA AGNS LALNQDIQEE QVVPARYRQE FLTIAWEQVH LRSIFPFQYF SIGASLIPFI EHNDANRALM SSNMQRQAVP LSRSEK CIV GTGLERQAAL DSGALAIAER EGRVVYTNTD KILLAGNGDI LSIPLVIYQR SNKNTCMHQK LQVPRGKCIK KGQILAD GA ATVGGELALG KNVLVAYMPW EGYNSEDAVL ISERLVYEDI YTSFHIRKYE IQTHVTSQGP EKVTNEIPHL EAHLLRNL D KNGIVMLGSW VETGDILVGK LTPQVVKESS YAPEDRLLRA ILGIQVSTSK ETCLKLPIGG RGRVIDVRWI QKRGGSSYN PETIRVYILQ KREIKVGDKV AGRHGNKGII SKILPRQDMP YLQDGRSVDM VFNPLGVPSR MNVGQIFECS LGLAGSLLDR HYRIAPFDE RYEQEASRKL VFSELYEASK QTANPWVFEP EYPGKSRIFD GRTGNPFEQP VIIGKPYILK LIHQVDDKIH G RSSGHYAL VTQQPLRGRA KQGGQRVGEM EVWALEGFGV AHILQEMLTY KSDHIRARQE VLGTTIIGGT IPNPEDAPES FR LLVRELR SLALELNHFL VSEKNFQINR KEA

UniProtKB: DNA-directed RNA polymerase subunit beta

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Macromolecule #3: DNA-directed RNA polymerase subunit gamma

MacromoleculeName: DNA-directed RNA polymerase subunit gamma / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Nicotiana tabacum (common tobacco)
Molecular weightTheoretical: 79.367297 KDa
SequenceString: MNNNFSSMID RYKHQQLRIG SVSPQQISAW ATKILPNGEI VGEVTKPYTF HYKTNKPEKD GLFCERIFGP IKSGICACGN YRVIGDEKE DPKFCEQCGV EFVDSRIRRY QMGYIKLACP VTHVWYLKRL PSYIANLLDK PLKELEGLVY CDFSFARPIT K KPTFLRLR ...String:
MNNNFSSMID RYKHQQLRIG SVSPQQISAW ATKILPNGEI VGEVTKPYTF HYKTNKPEKD GLFCERIFGP IKSGICACGN YRVIGDEKE DPKFCEQCGV EFVDSRIRRY QMGYIKLACP VTHVWYLKRL PSYIANLLDK PLKELEGLVY CDFSFARPIT K KPTFLRLR GLFEYEIQSW KYSIPLFFTT QGFDTFRNRE ISTGAGAIRE QLADLDLRII IENSLVEWEE LGEEGHTGNE WE DRKVGRR KDFLVRRVEL AKHFIRTNIE PEWMVLCLLP VLPPELRPII QIDGGKLMSS DINELYRRVI YRNNTLTDLL TTS RSTPGE LVMCQEKLVQ EAVDTLLDNG IRGQPMRDGH NKVYKSFSDV IEGKEGRFRE TLLGKRVDYS GRSVIVVGPS LSLH RCGLP REIAIELFQT FVIRGLIRQH LASNIGVAKS KIREKEPIVW EILQEVMQGH PVLLNRAPTL HRLGIQAFQP VLVEG RAIC LHPLVCKGFN ADFDGDQMAV HVPLSLEAQV EARLLMFSHM NLLSPAIGDP ISVPTQDMLI GLYVLTSGNH RGICVN RYN PCNRRNYQNQ KRSDNSHYKY TKEPFFSNSY DAIGAYRQKR INLDSPLWLR WRLDQRVIAS RETPIEVHYE SLGTFYE IY GHYLIVRSLK KQILFIYIRT TVGHIALYRE IEEAIQGFSR AYSSGT

UniProtKB: DNA-directed RNA polymerase subunit gamma

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Macromolecule #4: PAP1(pTAC3)

MacromoleculeName: PAP1(pTAC3) / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Nicotiana tabacum (common tobacco)
Molecular weightTheoretical: 101.413672 KDa
SequenceString: MAGISIHYLP FNSKFTLPIP RPSISRSIKA SVSSQPRKTR RRKQTQQQQH QLKTAEDDGS MASGTEKVLR LVFMEELMER ARNADSKGV SQVIYDMIAA GLSPGPRSFH GFVVSHVLNR DNDGAMHALR RELSEGLRPL HETFLALVRL FGAKGLATRG L EILAAMEK ...String:
MAGISIHYLP FNSKFTLPIP RPSISRSIKA SVSSQPRKTR RRKQTQQQQH QLKTAEDDGS MASGTEKVLR LVFMEELMER ARNADSKGV SQVIYDMIAA GLSPGPRSFH GFVVSHVLNR DNDGAMHALR RELSEGLRPL HETFLALVRL FGAKGLATRG L EILAAMEK LKYDIRQAWL VLVEELVRSN HLEDANKVFL KGAEGGLRAT DEIYDLLIEQ DCKVGDHSNA LTIAYEMEAA GR MATTFHF NCLLSVQATC GIPEIAFATF ENMEYGEDHM KPDTETYNWV IQAYTRAESY DRVQDVAELL GMMVEDHKRV QPN VRTYAL LVECFTKYCV VREAIRHFRG LKNFEGGTQV LYNDGKYGDP LSLYLRALCR EGRIVELLEA LEAMAKDNQP IPPR AMILS RKYRTLVSSW IEPLQEEAEL GYEIDYIARY VAEGGLTGDR KRWVPRRGKT PLDPDAEGFI YSNPRETSFK QRCLE EWRL HHRKLLKTLH NEGPSILGKI SESDYIRLVE RLRKIIKGPE QNALKPKAAS KMIVSELKEE LEAQGLPTDG TRNVLY QRV QKARRINRSR GRPLWVPPVE EEEEEVDEEL DELISRIKLH EGNTEFWKRR FLGEGLNENH VQQSEIIDLE PTDVVDD TD DAVDDISKEA EDDEAEDDEA QDEEEEVEQA ESQPEVGDRK DKEVEAAKPL QMIGVQLLKD SDQTASSSRK SRRRLSRV A AVDDDDDDWF PLDIQEAFVE MRKRNIFDVS DMYTITDAWG WTWEKEIKNK APQRWSQEWE VELGIKVMTK VIELGGTPT IGDCAVILRA AVRAPMPSAF LNILQTTHSL GYVFGSPLYD EIITLCLDLG ELDAAIAIVA DLETSGIKVP DETLDRVISA RQSSDTPVN GSQ

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Macromolecule #5: Pentatricopeptide repeat-containing protein At1g74850, chloroplas...

MacromoleculeName: Pentatricopeptide repeat-containing protein At1g74850, chloroplastic-like
type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Nicotiana tabacum (common tobacco)
Molecular weightTheoretical: 96.666289 KDa
SequenceString: MSLSCNSFSP VFTPVPPSHR FLFPAKIPNY GKLSPVHRRL LLTVAVRAKP KELILGNPTV TVEKGKYSYD VETLINKLSS LPPRGSIAR CLDTFKNKLS LTDFSHVFKE FAARGDWQRS LRLFKYMQRQ IWCKPNEHIY TLMIGILGRE GLLDKAFEIF D EMPSHSVA ...String:
MSLSCNSFSP VFTPVPPSHR FLFPAKIPNY GKLSPVHRRL LLTVAVRAKP KELILGNPTV TVEKGKYSYD VETLINKLSS LPPRGSIAR CLDTFKNKLS LTDFSHVFKE FAARGDWQRS LRLFKYMQRQ IWCKPNEHIY TLMIGILGRE GLLDKAFEIF D EMPSHSVA RTVFSYTAII NAYGRNGQYG TSLQLLEKMK QEKIIPSILT YNTVINSCAR GGHEWEGLLS LFAEMRHEGI QP DLVTYNT LLSACSSRGL GDEAEMVFRT MNEAGILPDV TTYSYLVDTF GKLGKLEKVS ELLMEMEAGG TSPEVTSYNV LLE AYAHSG SMKEAMDVFR QMQTAGCVAN AETYSVLLNL YGKNGRYDQV RDLFLEMKMS NTEPDADTYN ILIQVFGEGG YFKE VVTLF HDMVEEKVEP NMETYEGLIY ACGKGGLHDD AKRILLHMNG QGLVPSSKVY TGVIEAYGQA ALYEEAVVAF NTMNE VGSR PMVETFNSLI HAFAKGGLYK ESEAIWFRMG EVGVPRNRDS FNGMIEGYRQ GGQFEEAIKA YVEMEKARCD PDERTL EAV LSVYCFAGLV DESEEQFQEI KSLGIQPSII CCCMMLAIYA KSERWNMAHE LLNDVMTNKT SDMHQVIGQM IHGDFDD EN NWQMVEYVFD KLNSEGCGLS MRFYNTLIEA LWWLGQKERA ARVLNEATKR GLFPELFRRN KLVWSVDVHR MWPGGACT A ISVWLNDMEE LFHKGEELPQ LASVVVVRGQ TEKSSITRDF PVAKAAYSFL KDTVSSSFCF PGWNKGRIVC QKTQLKRIF SSAEPSSGSS KGDRLIPLSN SPISLLGTQT SVSDAKRSES ANADSKRSTK SDSELMASSV

UniProtKB: Pentatricopeptide repeat-containing protein At1g74850, chloroplastic-like

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Macromolecule #6: Protein PLASTID TRANSCRIPTIONALLY ACTIVE 10-like

MacromoleculeName: Protein PLASTID TRANSCRIPTIONALLY ACTIVE 10-like / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Nicotiana tabacum (common tobacco)
Molecular weightTheoretical: 80.402969 KDa
SequenceString: MQTLQSSSLF FTFPSSKTLL KPQSSKFSSF SLFPSSLSKP SRPLTLHCFS SDEFPVGDDD AFLEAFGPKE KESEEEARRK NWVDRGWAP WEEILSPEAN FARKSLNEGE EVALQSPEAI EAFKMLSPNY RKKKISDMGI TEDEYYAKQF EIKGEIPEPL S TMWAGPLV ...String:
MQTLQSSSLF FTFPSSKTLL KPQSSKFSSF SLFPSSLSKP SRPLTLHCFS SDEFPVGDDD AFLEAFGPKE KESEEEARRK NWVDRGWAP WEEILSPEAN FARKSLNEGE EVALQSPEAI EAFKMLSPNY RKKKISDMGI TEDEYYAKQF EIKGEIPEPL S TMWAGPLV VRHVPPRDWP PRGWEVDKKE LEFIRETHKL QSVRVDYDKV EEMVKMETDD MGLDRYKMFL KQYNEWVAAN KD RLEKESY KYDQDYYPGR RKRGKDYQDG MYELPFYYPG QICAGKVTAI HLYQGAFVDI GGVHDGWVPI KRNDWYWIRH HIK VGMHVI VEILAKRDPY RFRFPIEMRF IDPNIDHLIF NRFDFAPIFH RDEDTNLDEL RRDCGRQPLP RKDPGVKVEE EPLL SNHPY VDKLWQIHNA EQMILDDMEA NPVKYKGKNL TELTDDEDFD EENRIEYSKA YYKKALLPKM ITKVSVKELD LEAAF AERQ HHNKLRMEAQ ERGEVYKIPK LRRNIEMDEY DFIHWRRSLE EREAMLRDIS CRRALGLPLE EPGRYVDPSA FGKDQY DPD SPLYRYDYWG EPKNSEKSKQ ERMTDVHNKS IVGKGTVWYE MAYEDAVKER MQMEAQGIVR ELYDEDSDSD EVGTDDD DD DEEDFDYSIL GDPSANVSNQ PYVNGTESRL SDEGMFEDKS

UniProtKB: Protein PLASTID TRANSCRIPTIONALLY ACTIVE 10-like

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Macromolecule #7: superoxide dismutase

MacromoleculeName: superoxide dismutase / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Nicotiana tabacum (common tobacco)
Molecular weightTheoretical: 30.420742 KDa
SequenceString: MSWSSCNSLC TSSSRQLLVT TDLSSQLNIP SRLSNVKRSL HGHHGVPKVF AYYGLKTPPY KLDALEPYMS QKMLEVHWGE HHRSYVEAL NKQLEKNDIL YGCTMEELIK VTYNNGNPLP EFSDAAQVWN HDFFWESMQP GGGDMPKLGL LQQIEKDFGS F TNFREKFT ...String:
MSWSSCNSLC TSSSRQLLVT TDLSSQLNIP SRLSNVKRSL HGHHGVPKVF AYYGLKTPPY KLDALEPYMS QKMLEVHWGE HHRSYVEAL NKQLEKNDIL YGCTMEELIK VTYNNGNPLP EFSDAAQVWN HDFFWESMQP GGGDMPKLGL LQQIEKDFGS F TNFREKFT EAALALFGSG WIWLVLKREE KRLAIVKTSN AVNPLVWNDI PLIGLDLWEH AYYLDYKNDK AKYVNVFMNH LV SWDAALG RMARAQAFVN LGEPKIPVA

UniProtKB: superoxide dismutase

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Macromolecule #8: Protein PLASTID TRANSCRIPTIONALLY ACTIVE 12-like

MacromoleculeName: Protein PLASTID TRANSCRIPTIONALLY ACTIVE 12-like / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Nicotiana tabacum (common tobacco)
Molecular weightTheoretical: 61.281289 KDa
SequenceString: MASVLSTSLY QDRGLRTMVS TDGFVPSFCC PYRKTLFTGT IPVSIWQFKL VSSSPFQKAP LAPCIKCENK EKDQASQGSF EQVSVERYP YHSYMDSTSG QLEPASGARA SIPGQEYWPE GTASRVRAAR APEPTGTSTG TPSYGKNPGS RRKKYKASAA A SKPSEINI ...String:
MASVLSTSLY QDRGLRTMVS TDGFVPSFCC PYRKTLFTGT IPVSIWQFKL VSSSPFQKAP LAPCIKCENK EKDQASQGSF EQVSVERYP YHSYMDSTSG QLEPASGARA SIPGQEYWPE GTASRVRAAR APEPTGTSTG TPSYGKNPGS RRKKYKASAA A SKPSEINI ISDDSAESPD NLPEEPKDLS SEYVIYQPEQ EEEELTGYEL DKRLGRPHPF IDPKTKKKIE KPLTSEELWW NW RKPEKEQ WSRWQRRRPD VETVFLKAMA ETGQVKLYGD HPTLTETALY RARRHLYKKE RLQAEKEKLE KIGPIAYYSE WVQ AWKKDT SREAIQKHFE ETGEDENTQL IEMFCHQTDR EYRIMMGTDI RIPRDPLAMR MREDQIKQIW GGDPVYPTIN YIQD PDEVI DYRGPDFHEP TPNMLAYLKE HGKIISREEL EKILAKEKTE EIEVAEIDEA MARAVDIGEN DDEEEGSDAE VEEGD EKIT RNWSVLKSNP ELRKSKEKPK KKDMSLEEAV DDSENLTDFL MDFDEDE

UniProtKB: Protein PLASTID TRANSCRIPTIONALLY ACTIVE 12-like

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Macromolecule #9: Fructokinase-like 1, chloroplastic

MacromoleculeName: Fructokinase-like 1, chloroplastic / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Nicotiana tabacum (common tobacco)
Molecular weightTheoretical: 55.77 KDa
SequenceString: MASIHFLPQS LPHHSHNSLT HFNFSSIFFS SKLPHRKNTL KCSSNDHSTT TQEPPKHSPR GPKKSSRTAK KTSKPQKDSN FETNIEKSN TQMELSKNAD DKIEKYDDGV DFPYPNPPLV ICFGAAQKEF VPTVRVSHEQ MHQDKYSEWK MLQWNPPEFV R APGGPPSN ...String:
MASIHFLPQS LPHHSHNSLT HFNFSSIFFS SKLPHRKNTL KCSSNDHSTT TQEPPKHSPR GPKKSSRTAK KTSKPQKDSN FETNIEKSN TQMELSKNAD DKIEKYDDGV DFPYPNPPLV ICFGAAQKEF VPTVRVSHEQ MHQDKYSEWK MLQWNPPEFV R APGGPPSN VAISHVRLGG RAAFMGKVGN DEFGQEMVLL MNKEKVQTRA VKFDDSMRTG CTHMKIKFED GKMKVEKVKE PA EDSLSSS ELNLDVLKEA RIFHFNSEVL TSSSMRSTLF KAISLSKKFG GLVFFDLNLP LPLWRSRDET RNLIKEAWEQ ANI IEVSRQ ELEFLLDEEH YERKRNYRPQ YFAENFEQTK QRRDYYHYTP AEIAPLWHDG LKLLFVTDGT LRLHYYSPSF DGVV VGTED VLITPFTCDR TGSGDAVVAG IMRKLTTQPE MYHDQDVLER QLRFAIAAGI ISQWTIGAVR GFPTESATQN LKEQV YVPS MW

UniProtKB: Fructokinase-like 1, chloroplastic

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Macromolecule #10: Protein PLASTID TRANSCRIPTIONALLY ACTIVE 14-like isoform X2

MacromoleculeName: Protein PLASTID TRANSCRIPTIONALLY ACTIVE 14-like isoform X2
type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Nicotiana tabacum (common tobacco)
Molecular weightTheoretical: 58.183145 KDa
SequenceString: MVSSTILIQQ PTNLFHQPEF QHQLWRQGCA APLQKQPIII CSSNKGRSRP LRVTASANGA ATSSTLEGYD SSPTPSAFPL FTPPSQPHD TPASQLELAD PDFYKIGYVR SFRAYGIEFR EGPDGYGVFA SRDVEPLRRA RVIMEIPLEL MLTISKKLPW M FFPDIIPV ...String:
MVSSTILIQQ PTNLFHQPEF QHQLWRQGCA APLQKQPIII CSSNKGRSRP LRVTASANGA ATSSTLEGYD SSPTPSAFPL FTPPSQPHD TPASQLELAD PDFYKIGYVR SFRAYGIEFR EGPDGYGVFA SRDVEPLRRA RVIMEIPLEL MLTISKKLPW M FFPDIIPV GHPIFDIINS TNPETDSDLR LACLLLYAFD CKDNFWQLYG DFLPSDDECT SFLLATEEDL LELQDEKLAS TM REQQQRA LEFWEKNWHS AVPLKIKRLA RDPERFIWAM CIAQSRSINL QMRIGALVQD ANLLVPYADM MNHSFQPNCF FHW RFKDRM LEVMINAGQR IRKGDEMTVD YMAGQKNNFF MQRYGFSSPV NPWDVIHFTG DAKIHLDTFL SVFNISGLPG EYYH NSRLS NDGDSFVDGA IIAAARTLPT WSDGDLPPIP SLERKAVKEL QEECHQMLAE FPTTSDEDQK ILDSMPDCRR TLEAA IKYR LHRKLLIEKV IQALDIYQDR ILF

UniProtKB: Protein PLASTID TRANSCRIPTIONALLY ACTIVE 14-like isoform X2

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Macromolecule #11: PAP8(pTAC6)

MacromoleculeName: PAP8(pTAC6) / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Nicotiana tabacum (common tobacco)
Molecular weightTheoretical: 38.179574 KDa
SequenceString: MSAAQLFFPL PPNLSTFFTT PSSQALLTIS FVKPISNNPN SVKQSFTTKR RRDFRVFADD EDADGGGPDD YDMDEDEVEE ADNKKDFDV DYDTLLGGAS LAVAATGDDI AMVHSSSFVF TQGWNSEKIV DYRINEEEFH KISLLDCDFF IRKPPDPDND V YDFREMYV ...String:
MSAAQLFFPL PPNLSTFFTT PSSQALLTIS FVKPISNNPN SVKQSFTTKR RRDFRVFADD EDADGGGPDD YDMDEDEVEE ADNKKDFDV DYDTLLGGAS LAVAATGDDI AMVHSSSFVF TQGWNSEKIV DYRINEEEFH KISLLDCDFF IRKPPDPDND V YDFREMYV TPPDTDIYAI PRVLAPMPQK YIRCAMSDYG CYNVTEPPID APRDPMYKSE REVSKVFLTK HYRNRRAGDP EF ALDFEEI YVIDSKTKSI TRAKVVVTVP GGRNRDRKND LLVIRDNGNS FKIIPSEERD DPTTVIEKEE WKKSRQDMER HLR KLRDFS VSNWF

UniProtKB: Uncharacterized protein LOC107760033

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Macromolecule #12: superoxide dismutase

MacromoleculeName: superoxide dismutase / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Nicotiana tabacum (common tobacco)
Molecular weightTheoretical: 34.825203 KDa
SequenceString: MMAATASTTL TSAFLPFQGF RESCRTLNWR THKKQFARKA GPVKVTAKFE LMPPPYPMNA LEPHMSHTTF EYHWGKHHRA YVDNLNKQI DGTELDGMTL EDIILITYNR GDLLPPFNNA AQAWNHQFFW ESMKPSGGGK PSGELLQLIN RDFGSFEAFV K EFKAAAAT ...String:
MMAATASTTL TSAFLPFQGF RESCRTLNWR THKKQFARKA GPVKVTAKFE LMPPPYPMNA LEPHMSHTTF EYHWGKHHRA YVDNLNKQI DGTELDGMTL EDIILITYNR GDLLPPFNNA AQAWNHQFFW ESMKPSGGGK PSGELLQLIN RDFGSFEAFV K EFKAAAAT QFGSGWAWLA YKANRLNVGN TSNPHPTDED KKLVVVKTPN AVNPLVWDYS PLLTIDVWEH AYYLDFRNRR PD YISIFME KLVSWEAVSS RLEVAKAKAA EREEEEERKK REKEEEYKAG GEVREMYVES TDSDAE

UniProtKB: superoxide dismutase

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Macromolecule #13: Thioredoxin-like protein CITRX1, chloroplastic

MacromoleculeName: Thioredoxin-like protein CITRX1, chloroplastic / type: protein_or_peptide / ID: 13 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Nicotiana tabacum (common tobacco)
Molecular weightTheoretical: 20.142209 KDa
SequenceString:
MQAATLSFHP LAPPPQTSAC HFSSNQRKYS LFSYTCPTPR PSLLSTQTLS RKSICKPPAV ATGKYVREDY LVKKVSAKDI QELIKGERN VPLIIDFYAT WCGPCILMAQ ELEMLAVEYE SNALIVKVDT DDEYEFARDM QVRGLPTLYF ISPDPNKDAI R TEGLIPIQ MMRDIINNDL

UniProtKB: Thioredoxin-like protein CITRX1, chloroplastic

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Macromolecule #14: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2, 6-diaminopimelate l...

MacromoleculeName: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2, 6-diaminopimelate ligase-like
type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Nicotiana tabacum (common tobacco)
Molecular weightTheoretical: 85.288945 KDa
SequenceString: MPFTLFSLPP FPSLLHHNPP PLQFKPFTSL HHLRLKYPTT LTTVSAIGAD GKYYPNPSDD DPPEAPEDSM HGVNKFQQIQ RQAAKAKKQ QEELFKKEQS IFVNALADVE DAPDNPALND NDNSGDDLFG EIDKAIALKR KEFVKQGLLK PNPPKNSTLV E SEVENVDE ...String:
MPFTLFSLPP FPSLLHHNPP PLQFKPFTSL HHLRLKYPTT LTTVSAIGAD GKYYPNPSDD DPPEAPEDSM HGVNKFQQIQ RQAAKAKKQ QEELFKKEQS IFVNALADVE DAPDNPALND NDNSGDDLFG EIDKAIALKR KEFVKQGLLK PNPPKNSTLV E SEVENVDE LEPEEVVDLE EIDGLSGLAE IEESDEEKSD FEVSDDMGKS EFSDLSSFDI DFDEFGKTKP RIVEPKFRMS LA ELLDESR VVPLSVYGDL EVGISGVQHD SRLVESGDLF VCCVGRKTDG HLYLSEADKR GAVAVVASKE IDIEETLGCK ALV IVEDTN AVLAVLAASF YRHPSKSMTL IGITGTNGKT TTSYLIKAMY EAMGLRTGML STVGYYIYGD NKLESPHTTP DAVL VQKLM AKMVHNGTEA LVMEASSHGL ALGRCDKVDF DIAVFTNLTR DHLDFHGTEE EYRDAKAKLF ARMVDPARHR KIVNI DDPN AAFFLAQGNP DVPIVTFAME NKSADVHPLK FQLSLFETQV LVNTPQGILE ISSGLLGRHN IYNILAAVAV GIAVGA PLE DIVKGIEEVD AVPGRCELID EEQAFGVIVD YAHTPDALSR LLDYVRELGP RRVITVFGCA GESDRGKRPI MAKIATD KS DVTILTSDNP KTEDPLDILD DMLAGVGWSM QDYLKHGEND YYPPLPNGHR LFLHDIRRVA VRCAVAMGEE GDIVVVAG K GHETYQIEGD KKEFFDDREE CREALQYVDE LHQAGIDTSE FPWRLPESH

UniProtKB: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2, 6-diaminopimelate ligase-like

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Macromolecule #15: Protein PLASTID TRANSCRIPTIONALLY ACTIVE 7-like isoform X1

MacromoleculeName: Protein PLASTID TRANSCRIPTIONALLY ACTIVE 7-like isoform X1
type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Nicotiana tabacum (common tobacco)
Molecular weightTheoretical: 19.2549 KDa
SequenceString:
MAASTLTFSG FSTLQTFPKI AAVENTKRVN FSIRSQSASN SKNESRGGRR IWRRRKLTKK DETLDAKMER IPFLEEQVRK IRDGGKLLT MDIHRLLLNE DNRFDFVNEI AAEAKQYVEN NRDEYGAKKA ILHVLSNRMN DAGVYRAEAY MESDPFKPGP G YMKDELL

UniProtKB: Protein PLASTID TRANSCRIPTIONALLY ACTIVE 7-like isoform X1

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Macromolecule #16: PAP13(pTAC18)

MacromoleculeName: PAP13(pTAC18) / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Nicotiana tabacum (common tobacco)
Molecular weightTheoretical: 17.054311 KDa
SequenceString:
MASFITMPAL SYLSTNTSSL ERTNFRPSSR GYQGVRAMRT EKPLEELYNV RVERNVTKDR LMELGVPRWS MWKTGKCKLP WDWHVDQLV YIEEGEVRVV PEGSQRFMQF VAGDLVRYPK WFEADLYFND FYQERYSFRA YGDS

UniProtKB: Uncharacterized protein LOC107779769

+
Macromolecule #20: DNA-directed RNA polymerase subunit beta''

MacromoleculeName: DNA-directed RNA polymerase subunit beta'' / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Nicotiana tabacum (common tobacco)
Molecular weightTheoretical: 157.057734 KDa
SequenceString: MAERANLVFH NKAINGTAMK RLISRLIDHF GMAYTSHILD QVKTLGFQQA TATSISLGID DLLTIPSKGW LVQDAEQQSL ILEKHHHYG NVHAVEKLRQ SIEIWYATSE YLRQEMNPNF RMTDPFNPVH IMSFSGARGN ASQVHQLVGM RGLMSDPQGQ M IDLPIQSN ...String:
MAERANLVFH NKAINGTAMK RLISRLIDHF GMAYTSHILD QVKTLGFQQA TATSISLGID DLLTIPSKGW LVQDAEQQSL ILEKHHHYG NVHAVEKLRQ SIEIWYATSE YLRQEMNPNF RMTDPFNPVH IMSFSGARGN ASQVHQLVGM RGLMSDPQGQ M IDLPIQSN LREGLSLTEY IISCYGARKG VVDTAVRTSD AGYLTRRLVE VVQHIVVRRT DCGTARGISV SPRNGMMPER IF IQTLIGR VLADDIYMGP RCIATRNQDI GIGLVNRFIT FRAQPISIRT PFTCRSTSWI CRLCYGRSPT HGDLVELGEA VGI IAGQSI GEPGTQLTLR TFHTGGVFTG GTAEHVRAPS NGKIKFNEDL VHPTRTRHGH PAFLCSIDLY VTIESEDILH NVNI PPKSL LLVQNDQYVE SEQVIAEIRA GISTLNFKEK VRKHIYSDSD GEMHWSTDVY HAPEFTYGNV HLLPKTSHLW ILLGR PCRS SLVYLSIHKD QDQMNAHFLS GKRRYTSNLS VTNDQARQKL FSSDFSGKKE DRIPDYSDLN RIICAGQYNL VYSPIL HEN SDLLSKRRRN KFIIPLHSIQ ELENELMPCS GISIEIPVNG IFRRNSILAY FDDPRYRRKS SGIIKYGTVE THSVIKK ED LLEYRGVKEF RPKYQMKVDR FFFIPEEVHI LPGSSSIMVR NNSIVGVDTQ ITLNLRSRVG GLVRVERKKK RIELKIFS G DIHFPGETDK ISRHTGVLIP PGTGKRNSKE SKKVKNWIYV QRITPSKKKF FVLVRPVVTY EITDGINLAT LFPPDPLQE RDNVQLRIVN YILYGNGKPI RGISDTSIQL VRTCLVLNWN QDKKSSSCEE ARASFVEIRT NGLIRHFLRI NLVKSPISYI GKRNDPSGS GLLSDNGSDC TNINPFSSIY SYSKAKIQQS INQPQGTIHT LLNRNKECQS LIILSAANCS RMGPFKDVKY H SVIKKSIK KDPLIPIRNS LGPLGTSLPI ENFYSSYHLI THNQILVTNY LQLDNLKQTF QVIKFKYYLM DENGKIFNPD PC RNIILNP FNLNWYFLHH NYCEETSKII SLGQFICENV CIAKNGPPLK SGQVILVQVD SIVIRSAKPY LATPGATVHG HYG ETLYEG DTLVTFIYEK SRSGDITQGL PKVEQVLEVR SVDSISMNLE KRIEGWNKCI TRILGIPWGF LIGAELTIAQ SRIS LVNKI QQVYRSQGVQ IHNRHLEIIV RQITSKVLVS EDGMSNVFSP GELIGLLRAE RMGRALEEAI CYRVVLLGIT RASLN TQSF ISEASFQETA RVLAKAALRG RIDWLKGLKE NVVLGGVIPV GTGFKGLVHP SKQHNNIPLE TKKKNLFEGE MRDILF HHK KLFDSCLSKN FHDIPEQSFI GFNDS

UniProtKB: DNA-directed RNA polymerase subunit beta''

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Macromolecule #21: Fructokinase-like 2, chloroplastic

MacromoleculeName: Fructokinase-like 2, chloroplastic / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Nicotiana tabacum (common tobacco)
Molecular weightTheoretical: 72.534133 KDa
SequenceString: MAALSFSLLS TLPRQHLHWN FYPNMKVMQL QDLGLKNKWV LMAVSKEGTP EAIAKELSKT EVFGAGKKTV RISKRAPVMA RRKKVVETS NDDPVNVEEA ENTSGSTEEP KKTQRRTRKK KEIVESSFGD SISDAEGNVI DAEAVTEPES SAKPKKTRRT R KKKETVVS ...String:
MAALSFSLLS TLPRQHLHWN FYPNMKVMQL QDLGLKNKWV LMAVSKEGTP EAIAKELSKT EVFGAGKKTV RISKRAPVMA RRKKVVETS NDDPVNVEEA ENTSGSTEEP KKTQRRTRKK KEIVESSFGD SISDAEGNVI DAEAVTEPES SAKPKKTRRT R KKKETVVS TFEDSTLDVE GNVTDEEVLP TSGSSEGSVE IRKRTSKKAA SSSSSLEKEP TQKVTRRRRK KVNNLEDEGS QT ELSDIEE ELHVANADAD SEEELDFDGG EDISFSYGWP PLVCCFGAAQ HAFVPSGRPS NRLVDHEWHE RMKDAIWDPE KFT RAPGGC SSNVAVALAS LGGKVAFMGK LGDDDFGQSL VYFMNINKVQ TRSVRLDSKK ATAITHMKIG KRGGLRMTTT KPSA EDSLL KSEINIDVLK EAKMFYFNTF SMLDPNMRLT TLRATKISKK LGGVVFYDVN LPFPLWESGD KAKTFIQQAW DLADI IEVT KQELEFLCGI KPSERFDTKD NDRSKFTHYP PEVIAPLWHE NLKVLFVTNG TSKIHYYTKE HNSAVLGLED VPLTPY TSD MSASGDGIIA GIIRMLTVQP HLMTDKGYLE RTLKYAISCG VVDQWLQARR LGYPPKEGME DDVVPDDHGI KSVTERE YR ILVPVS

UniProtKB: Fructokinase-like 2, chloroplastic

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Macromolecule #17: DNA (24-mer)

MacromoleculeName: DNA (24-mer) / type: dna / ID: 17 / Number of copies: 1 / Classification: DNA
Source (natural)Organism: synthetic construct (others)
Molecular weightTheoretical: 7.301712 KDa
SequenceString:
(DC)(DG)(DT)(DT)(DA)(DT)(DT)(DC)(DA)(DC) (DC)(DA)(DT)(DT)(DA)(DG)(DC)(DG)(DT)(DC) (DT)(DT)(DG)(DT)

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Macromolecule #18: DNA (24-mer)

MacromoleculeName: DNA (24-mer) / type: dna / ID: 18 / Number of copies: 1 / Classification: DNA
Source (natural)Organism: synthetic construct (others)
Molecular weightTheoretical: 7.386807 KDa
SequenceString:
(DA)(DC)(DA)(DA)(DC)(DT)(DG)(DC)(DG)(DA) (DT)(DT)(DA)(DC)(DG)(DT)(DG)(DA)(DA)(DT) (DA)(DA)(DC)(DG)

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Macromolecule #19: RNA (27-mer)

MacromoleculeName: RNA (27-mer) / type: rna / ID: 19 / Number of copies: 1
Source (natural)Organism: synthetic construct (others)
Molecular weightTheoretical: 8.72028 KDa
SequenceString:
CUAAUAACAG AGGACGUGGU AAUCGCA

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Macromolecule #22: ZINC ION

MacromoleculeName: ZINC ION / type: ligand / ID: 22 / Number of copies: 1 / Formula: ZN
Molecular weightTheoretical: 65.409 Da

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Macromolecule #23: MAGNESIUM ION

MacromoleculeName: MAGNESIUM ION / type: ligand / ID: 23 / Number of copies: 1 / Formula: MG
Molecular weightTheoretical: 24.305 Da

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Macromolecule #24: FE (III) ION

MacromoleculeName: FE (III) ION / type: ligand / ID: 24 / Number of copies: 2 / Formula: FE
Molecular weightTheoretical: 55.845 Da

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 7.5
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Image recordingFilm or detector model: GATAN K3 (6k x 4k) / Average electron dose: 57.8 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 1.8 µm / Nominal defocus min: 1.0 µm
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

+
Image processing

Startup modelType of model: NONE
Final reconstructionResolution.type: BY AUTHOR / Resolution: 2.8 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 88607
Initial angle assignmentType: MAXIMUM LIKELIHOOD
Final angle assignmentType: MAXIMUM LIKELIHOOD
FSC plot (resolution estimation)

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