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TitleCryo-EM structures of the plant plastid-encoded RNA polymerase.
Journal, issue, pagesCell, Vol. 187, Issue 5, Page 1127-1144.e21, Year 2024
Publish dateFeb 29, 2024
AuthorsXiao-Xian Wu / Wen-Hui Mu / Fan Li / Shu-Yi Sun / Chao-Jun Cui / Chanhong Kim / Fei Zhou / Yu Zhang /
PubMed AbstractChloroplasts are green plastids in the cytoplasm of eukaryotic algae and plants responsible for photosynthesis. The plastid-encoded RNA polymerase (PEP) plays an essential role during chloroplast ...Chloroplasts are green plastids in the cytoplasm of eukaryotic algae and plants responsible for photosynthesis. The plastid-encoded RNA polymerase (PEP) plays an essential role during chloroplast biogenesis from proplastids and functions as the predominant RNA polymerase in mature chloroplasts. The PEP-centered transcription apparatus comprises a bacterial-origin PEP core and more than a dozen eukaryotic-origin PEP-associated proteins (PAPs) encoded in the nucleus. Here, we determined the cryo-EM structures of Nicotiana tabacum (tobacco) PEP-PAP apoenzyme and PEP-PAP transcription elongation complexes at near-atomic resolutions. Our data show the PEP core adopts a typical fold as bacterial RNAP. Fifteen PAPs bind at the periphery of the PEP core, facilitate assembling the PEP-PAP supercomplex, protect the complex from oxidation damage, and likely couple gene transcription with RNA processing. Our results report the high-resolution architecture of the chloroplast transcription apparatus and provide the structural basis for the mechanistic and functional study of transcription regulation in chloroplasts.
External linksCell / PubMed:38428393
MethodsEM (single particle)
Resolution2.7 - 3.0 Å
Structure data

37386

8w9z

EMDB-37386, PDB-8w9z:
The cryo-EM structure of the Nicotiana tabacum PEP-PAP
Method: EM (single particle) / Resolution: 3.0 Å

37387

8wa0

EMDB-37387, PDB-8wa0:
The cryo-EM structure of the Nicotiana tabacum PEP-PAP-TEC1
Method: EM (single particle) / Resolution: 2.7 Å

37388

8wa1

EMDB-37388, PDB-8wa1:
The cryo-EM structure of the Nicotiana tabacum PEP-PAP-TEC2
Method: EM (single particle) / Resolution: 2.8 Å

Chemicals

ChemComp-ZN:
Unknown entry

ChemComp-MG:
Unknown entry

ChemComp-FE:
Unknown entry

Source
  • nicotiana tabacum (common tobacco)
  • synthetic construct (others)
KeywordsTRANSCRIPTION / PEP / PAP

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