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- EMDB-3637: The cryo-EM structure of hibernating 100S ribosome dimer from pat... -

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Basic information

Entry
Database: EMDB / ID: EMD-3637
TitleThe cryo-EM structure of hibernating 100S ribosome dimer from pathogenic Staphylococcus aureus
Map datacryo-EM maps of S. aureus 100S complex (unsharpened)
Sample
  • Complex: 100S complex of Staphylococcus aureus
    • Complex: 30S subunit
      • RNA: x 1 types
      • Protein or peptide: x 21 types
    • Complex: 50S subunit
      • RNA: x 2 types
      • Protein or peptide: x 28 types
    • Complex: 23S and 5S rRNA
    • Complex: 50S ribosomal proteins
    • Complex: 16S rRNA
    • Complex: 30S ribosomal proteins
Function / homology
Function and homology information


large ribosomal subunit / regulation of translation / ribosomal small subunit assembly / small ribosomal subunit / 5S rRNA binding / transferase activity / cytosolic small ribosomal subunit / ribosomal large subunit assembly / cytosolic large ribosomal subunit / tRNA binding ...large ribosomal subunit / regulation of translation / ribosomal small subunit assembly / small ribosomal subunit / 5S rRNA binding / transferase activity / cytosolic small ribosomal subunit / ribosomal large subunit assembly / cytosolic large ribosomal subunit / tRNA binding / rRNA binding / ribosome / structural constituent of ribosome / ribonucleoprotein complex / translation / mRNA binding / zinc ion binding / cytoplasm
Similarity search - Function
Ribosome hibernation promoting factor, long/plastid / Sigma 54 modulation/S30EA ribosomal protein, C-terminal / Sigma 54 modulation/S30EA ribosomal protein, C-terminal domain superfamily / Sigma 54 modulation/S30EA ribosomal protein C terminus / Ribosomal protein L31 type B / : / Ribosome hibernation promoting factor/RaiA / Ribosome hibernation promotion factor-like / Sigma 54 modulation protein / S30EA ribosomal protein / Ribosomal protein L25, long-form ...Ribosome hibernation promoting factor, long/plastid / Sigma 54 modulation/S30EA ribosomal protein, C-terminal / Sigma 54 modulation/S30EA ribosomal protein, C-terminal domain superfamily / Sigma 54 modulation/S30EA ribosomal protein C terminus / Ribosomal protein L31 type B / : / Ribosome hibernation promoting factor/RaiA / Ribosome hibernation promotion factor-like / Sigma 54 modulation protein / S30EA ribosomal protein / Ribosomal protein L25, long-form / Ribosomal protein L25, beta domain / Ribosomal protein L25, C-terminal / Ribosomal protein TL5, C-terminal domain / : / Ribosomal protein S14, type Z / Ribosomal protein S21, conserved site / Ribosomal protein S21 signature. / Ribosomal protein S21 superfamily / Ribosomal protein S21 / Ribosomal protein S21 / Ribosomal protein L31 signature. / Ribosomal protein L31 / Ribosomal protein L31 superfamily / Ribosomal protein L31 / : / Ribosomal protein L16 signature 1. / : / Ribosomal protein L6, conserved site / Ribosomal protein L6 signature 1. / Ribosomal protein L16, conserved site / Ribosomal protein L16 signature 2. / Ribosomal protein L17 signature. / Ribosomal L25p family / Ribosomal protein L25 / Ribosomal protein L36 signature. / Ribosomal protein L28/L24 superfamily / Ribosomal protein L25/Gln-tRNA synthetase, N-terminal / Ribosomal protein L25/Gln-tRNA synthetase, anti-codon-binding domain superfamily / Ribosomal protein L32p, bacterial type / : / : / Ribosomal protein L28 / Ribosomal protein L35, conserved site / Ribosomal protein L35 signature. / Ribosomal protein L33, conserved site / Ribosomal protein L33 signature. / Ribosomal protein L35, non-mitochondrial / Ribosomal protein L5, bacterial-type / Ribosomal protein L18, bacterial-type / Ribosomal protein L6, bacterial-type / Ribosomal protein S14/S29 / Ribosomal protein L19, conserved site / Ribosomal protein L19 signature. / Ribosomal protein S3, bacterial-type / Ribosomal protein S6, conserved site / Ribosomal protein S6 signature. / Ribosomal protein S19, bacterial-type / Ribosomal protein S13, bacterial-type / Ribosomal protein L36 / Ribosomal protein L36 superfamily / Ribosomal protein L36 / Ribosomal protein S7, bacterial/organellar-type / Ribosomal protein S11, bacterial-type / Ribosomal protein S20 / Ribosomal protein S20 superfamily / Ribosomal protein S20 / Ribosomal protein L20 signature. / Ribosomal protein S9, bacterial/plastid / Ribosomal protein L27, conserved site / Ribosomal protein L27 signature. / Ribosomal protein S4, bacterial-type / 30S ribosomal protein S17 / Ribosomal protein S5, bacterial-type / Ribosomal protein L14P, bacterial-type / Ribosomal protein S6, plastid/chloroplast / Ribosomal protein L22, bacterial/chloroplast-type / Ribosomal protein L2, bacterial/organellar-type / Ribosomal protein S2, bacteria/mitochondria/plastid / Ribosomal protein L35 / Ribosomal protein L35 superfamily / Ribosomal protein L35 / Ribosomal protein L34, conserved site / Ribosomal L28 family / Ribosomal protein L34 signature. / Ribosomal protein L33 / Ribosomal protein L33 / Ribosomal protein L28/L24 / Ribosomal protein L18 / Ribosomal L18 of archaea, bacteria, mitoch. and chloroplast / Ribosomal protein L33 superfamily / Ribosomal protein L30, bacterial-type / Ribosomal protein L16 / Ribosomal protein S16 / Ribosomal protein S16 domain superfamily / Ribosomal protein S16 / : / L28p-like / Ribosomal protein L20 / Ribosomal protein S15, bacterial-type / Ribosomal protein L20
Similarity search - Domain/homology
Large ribosomal subunit protein bL36 / Large ribosomal subunit protein bL33 / Small ribosomal subunit protein uS7 / 50S ribosomal protein L27 / Small ribosomal subunit protein uS19 / Small ribosomal subunit protein uS12 / Large ribosomal subunit protein bL25 / : / Small ribosomal subunit protein uS9 / 50S ribosomal protein L32 ...Large ribosomal subunit protein bL36 / Large ribosomal subunit protein bL33 / Small ribosomal subunit protein uS7 / 50S ribosomal protein L27 / Small ribosomal subunit protein uS19 / Small ribosomal subunit protein uS12 / Large ribosomal subunit protein bL25 / : / Small ribosomal subunit protein uS9 / 50S ribosomal protein L32 / Large ribosomal subunit protein bL27 / Ribosome hibernation promotion factor / Small ribosomal subunit protein bS21 / Small ribosomal subunit protein bS20 / Large ribosomal subunit protein bL21 / Large ribosomal subunit protein bL35 / Large ribosomal subunit protein bL20 / Small ribosomal subunit protein uS4 / Large ribosomal subunit protein bL31B / Small ribosomal subunit protein bS6 / Small ribosomal subunit protein bS16 / Large ribosomal subunit protein bL28 / Small ribosomal subunit protein uS2 / Large ribosomal subunit protein bL19 / Small ribosomal subunit protein uS15 / Small ribosomal subunit protein uS17 / Large ribosomal subunit protein uL14 / Large ribosomal subunit protein uL24 / Large ribosomal subunit protein uL5 / Small ribosomal subunit protein uS14B / Small ribosomal subunit protein uS8 / Large ribosomal subunit protein uL6 / Small ribosomal subunit protein uS5 / Large ribosomal subunit protein uL30 / Large ribosomal subunit protein uL15 / Small ribosomal subunit protein uS13 / Small ribosomal subunit protein uS11 / Large ribosomal subunit protein bL17 / Large ribosomal subunit protein uL13 / Large ribosomal subunit protein uL29 / Small ribosomal subunit protein uS10 / Large ribosomal subunit protein uL3 / Large ribosomal subunit protein uL4 / Large ribosomal subunit protein uL23 / Large ribosomal subunit protein uL2 / Large ribosomal subunit protein uL22 / Small ribosomal subunit protein uS3 / Large ribosomal subunit protein uL16 / Large ribosomal subunit protein bL34 / Large ribosomal subunit protein uL18
Similarity search - Component
Biological speciesStaphylococcus aureus (bacteria)
Methodsingle particle reconstruction / cryo EM / Resolution: 6.76 Å
AuthorsMatzov D / Aibara S / Zimmerman E / Bashan A / Amunts A / Yonath A
CitationJournal: Nat Commun / Year: 2017
Title: The cryo-EM structure of hibernating 100S ribosome dimer from pathogenic Staphylococcus aureus.
Authors: Donna Matzov / Shintaro Aibara / Arnab Basu / Ella Zimmerman / Anat Bashan / Mee-Ngan F Yap / Alexey Amunts / Ada E Yonath /
Abstract: Formation of 100S ribosome dimer is generally associated with translation suppression in bacteria. Trans-acting factors ribosome modulation factor (RMF) and hibernating promoting factor (HPF) were ...Formation of 100S ribosome dimer is generally associated with translation suppression in bacteria. Trans-acting factors ribosome modulation factor (RMF) and hibernating promoting factor (HPF) were shown to directly mediate this process in E. coli. Gram-positive S. aureus lacks an RMF homolog and the structural basis for its 100S formation was not known. Here we report the cryo-electron microscopy structure of the native 100S ribosome from S. aureus, revealing the molecular mechanism of its formation. The structure is distinct from previously reported analogs and relies on the HPF C-terminal extension forming the binding platform for the interactions between both of the small ribosomal subunits. The 100S dimer is formed through interactions between rRNA h26, h40, and protein uS2, involving conformational changes of the head as well as surface regions that could potentially prevent RNA polymerase from docking to the ribosome.Under conditions of nutrient limitation, bacterial ribosomes undergo dimerization, forming a 100S complex that is translationally inactive. Here the authors present the structural basis for formation of the 100S complexes in Gram-positive bacteria, shedding light on the mechanism of translation suppression by the ribosome-silencing factors.
History
DepositionMar 17, 2017-
Header (metadata) releaseApr 5, 2017-
Map releaseOct 4, 2017-
UpdateNov 25, 2020-
Current statusNov 25, 2020Processing site: PDBe / Status: Released

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 0.023
  • Imaged by UCSF Chimera
  • Download
  • Surface view colored by cylindrical radius
  • Surface level: 0.023
  • Imaged by UCSF Chimera
  • Download
  • Surface view with fitted model
  • Atomic models: PDB-6fxc
  • Surface level: 0.023
  • Imaged by UCSF Chimera
  • Download
Movie viewer
Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

emd_3637.png

Downloads & links

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Map

FileDownload / File: emd_3637.map.gz / Format: CCP4 / Size: 244.1 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotationcryo-EM maps of S. aureus 100S complex (unsharpened)
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
1.61 Å/pix.
x 400 pix.
= 642. Å		1.61 Å/pix.
x 400 pix.
= 642. Å		1.61 Å/pix.
x 400 pix.
= 642. Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 1.605 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.023 / Movie #1: 0.023
Minimum - Maximum-0.021265034 - 0.059484284
Average (Standard dev.)0.0000687619 (-)
SymmetrySpace group: 0
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions400400400
Spacing400400400
CellA=B=C: 642.0 Å
α=β=γ: 90.0 °

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z1.6051.6051.605
M x/y/z400400400
origin x/y/z0.0000.0000.000
length x/y/z642.000642.000642.000
α/β/γ90.00090.00090.000
MAP C/R/S123
start NC/NR/NS000
NC/NR/NS400400400
D min/max/mean-0.0210.0590.000

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Supplemental data

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Additional map: ryo-EM maps of S. aureus 100S complex with...

Fileemd_3637_additional.map
Annotationryo-EM maps of S. aureus 100S complex with both large subunits masked out (unsharpened)
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: First half map of the masked S. aureus 100S complex

Fileemd_3637_half_map_1.map
AnnotationFirst half map of the masked S. aureus 100S complex
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: Second half map of the masked S. aureus 100S complex

Fileemd_3637_half_map_2.map
AnnotationSecond half map of the masked S. aureus 100S complex
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : 100S complex of Staphylococcus aureus

EntireName: 100S complex of Staphylococcus aureus
Components
  • Complex: 100S complex of Staphylococcus aureus
    • Complex: 30S subunit
      • RNA: 16S ribosomal RNA
      • Protein or peptide: 30S ribosomal protein S2
      • Protein or peptide: 30S ribosomal protein S3
      • Protein or peptide: 30S ribosomal protein S4
      • Protein or peptide: 30S ribosomal protein S5
      • Protein or peptide: 30S ribosomal protein S6
      • Protein or peptide: 30S ribosomal protein S7
      • Protein or peptide: 30S ribosomal protein S8
      • Protein or peptide: 30S ribosomal protein S9
      • Protein or peptide: 30S ribosomal protein S10
      • Protein or peptide: 30S ribosomal protein S11
      • Protein or peptide: 30S ribosomal protein S12
      • Protein or peptide: 30S ribosomal protein S13
      • Protein or peptide: 30S ribosomal protein S14 type Z
      • Protein or peptide: 30S ribosomal protein S15
      • Protein or peptide: 30S ribosomal protein S16
      • Protein or peptide: 30S ribosomal protein S17
      • Protein or peptide: 30S ribosomal protein S18
      • Protein or peptide: 30S ribosomal protein S19
      • Protein or peptide: 30S ribosomal protein S20
      • Protein or peptide: 30S ribosomal protein S21
      • Protein or peptide: Ribosome hibernation promotion factor
    • Complex: 50S subunit
      • RNA: 23S ribosomal RNA
      • RNA: 5S ribosomal RNA
      • Protein or peptide: 50S ribosomal protein L2
      • Protein or peptide: 50S ribosomal protein L3
      • Protein or peptide: 50S ribosomal protein L4
      • Protein or peptide: 50S ribosomal protein L5
      • Protein or peptide: 50S ribosomal protein L6
      • Protein or peptide: 50S ribosomal protein L13
      • Protein or peptide: 50S ribosomal protein L14
      • Protein or peptide: 50S ribosomal protein L15
      • Protein or peptide: 50S ribosomal protein L16
      • Protein or peptide: 50S ribosomal protein L17
      • Protein or peptide: 50S ribosomal protein L18
      • Protein or peptide: 50S ribosomal protein L19
      • Protein or peptide: 50S ribosomal protein L20
      • Protein or peptide: 50S ribosomal protein L21
      • Protein or peptide: 50S ribosomal protein L22
      • Protein or peptide: 50S ribosomal protein L23
      • Protein or peptide: 50S ribosomal protein L24
      • Protein or peptide: 50S ribosomal protein L25
      • Protein or peptide: 50S ribosomal protein L27
      • Protein or peptide: 50S ribosomal protein L28
      • Protein or peptide: 50S ribosomal protein L29
      • Protein or peptide: 50S ribosomal protein L30
      • Protein or peptide: 50S ribosomal protein L31 type B
      • Protein or peptide: 50S ribosomal protein L32
      • Protein or peptide: 50S ribosomal protein L33
      • Protein or peptide: 50S ribosomal protein L34
      • Protein or peptide: 50S ribosomal protein L35
      • Protein or peptide: 50S ribosomal protein L36
    • Complex: 23S and 5S rRNA
    • Complex: 50S ribosomal proteins
    • Complex: 16S rRNA
    • Complex: 30S ribosomal proteins

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Supramolecule #1: 100S complex of Staphylococcus aureus

SupramoleculeName: 100S complex of Staphylococcus aureus / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#52
Source (natural)Organism: Staphylococcus aureus (bacteria) / Strain: USA300_FPR3757

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Supramolecule #2: 30S subunit

SupramoleculeName: 30S subunit / type: complex / ID: 2 / Parent: 1 / Macromolecule list: #1-#22
Source (natural)Organism: Staphylococcus aureus (bacteria) / Strain: USA300_FPR3757

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Supramolecule #3: 50S subunit

SupramoleculeName: 50S subunit / type: complex / ID: 3 / Parent: 1 / Macromolecule list: #23-#52
Source (natural)Organism: Staphylococcus aureus (bacteria) / Strain: USA300_FPR3757

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Supramolecule #4: 23S and 5S rRNA

SupramoleculeName: 23S and 5S rRNA / type: complex / ID: 4 / Parent: 1 / Macromolecule list: #23-#24
Source (natural)Organism: Staphylococcus aureus (bacteria) / Strain: USA300_FPR3757

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Supramolecule #5: 50S ribosomal proteins

SupramoleculeName: 50S ribosomal proteins / type: complex / ID: 5 / Parent: 1 / Macromolecule list: #25-#52
Source (natural)Organism: Staphylococcus aureus (bacteria) / Strain: USA300_FPR3757

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Supramolecule #6: 16S rRNA

SupramoleculeName: 16S rRNA / type: complex / ID: 6 / Parent: 1 / Macromolecule list: #1
Source (natural)Organism: Staphylococcus aureus (bacteria) / Strain: USA300_FPR3757

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Supramolecule #7: 30S ribosomal proteins

SupramoleculeName: 30S ribosomal proteins / type: complex / ID: 7 / Parent: 1 / Macromolecule list: #2-#21
Source (natural)Organism: Staphylococcus aureus (bacteria) / Strain: USA300_FPR3757

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Macromolecule #1: 16S ribosomal RNA

MacromoleculeName: 16S ribosomal RNA / type: rna / ID: 1 / Number of copies: 2
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 502.975906 KDa
SequenceString: UUUUAUGGAG AGUUUGAUCC UGGCUCAGGA UGAACGCUGG CGGCGUGCCU AAUACAUGCA AGUCGAGCGA ACGGACGAGA AGCUUGCUU CUCUGAUGUU AGCGGCGGAC GGGUGAGUAA CACGUGGAUA ACCUACCUAU AAGACUGGGA UAACUUCGGG A AACCGGAG ...String:
UUUUAUGGAG AGUUUGAUCC UGGCUCAGGA UGAACGCUGG CGGCGUGCCU AAUACAUGCA AGUCGAGCGA ACGGACGAGA AGCUUGCUU CUCUGAUGUU AGCGGCGGAC GGGUGAGUAA CACGUGGAUA ACCUACCUAU AAGACUGGGA UAACUUCGGG A AACCGGAG CUAAUACCGG AUAAUAUUUU GAACCGCAUG GUUCAAAAGU GAAAGACGGU CUUGCUGUCA CUUAUAGAUG GA UCCGCGC UGCAUUAGCU AGUUGGUAAG GUAACGGCUU ACCAAGGCAA CGAUGCAUAG CCGACCUGAG AGGGUGAUCG GCC ACACUG GAACUGAGAC ACGGUCCAGA CUCCUACGGG AGGCAGCAGU AGGGAAUCUU CCGCAAUGGG CGAAAGCCUG ACGG AGCAA CGCCGCGUGA GUGAUGAAGG UCUUCGGAUC GUAAAACUCU GUUAUUAGGG AAGAACAUAU GUGUAAGUAA CUGUG CACA UCUUGACGGU ACCUAAUCAG AAAGCCACGG CUAACUACGU GCCAGCAGCC GCGGUAAUAC GUAGGUGGCA AGCGUU AUC CGGAAUUAUU GGGCGUAAAG CGCGCGUAGG CGGUUUUUUA AGUCUGAUGU GAAAGCCCAC GGCUCAACCG UGGAGGG UC AUUGGAAACU GGAAAACUUG AGUGCAGAAG AGGAAAGUGG AAUUCCAUGU GUAGCGGUGA AAUGCGCAGA GAUAUGGA G GAACACCAGU GGCGAAGGCG ACUUUCUGGU CUGUAACUGA CGCUGAUGUG CGAAAGCGUG GGGAUCAAAC AGGAUUAGA UACCCUGGUA GUCCACGCCG UAAACGAUGA GUGCUAAGUG UUAGGGGGUU UCCGCCCCUU AGUGCUGCAG CUAACGCAUU AAGCACUCC GCCUGGGGAG UACGACCGCA AGGUUGAAAC UCAAAGGAAU UGACGGGGAC CCGCACAAGC GGUGGAGCAU G UGGUUUAA UUCGAAGCAA CGCGAAGAAC CUUACCAAAU CUUGACAUCC UUUGACAACU CUAGAGAUAG AGCCUUCCCC UU CGGGGGA CAAAGUGACA GGUGGUGCAU GGUUGUCGUC AGCUCGUGUC GUGAGAUGUU GGGUUAAGUC CCGCAACGAG CGC AACCCU UAAGCUUAGU UGCCAUCAUU AAGUUGGGCA CUCUAAGUUG ACUGCCGGUG ACAAACCGGA GGAAGGUGGG GAUG ACGUC AAAUCAUCAU GCCCCUUAUG AUUUGGGCUA CACACGUGCU ACAAUGGACA AUACAAAGGG CAGCGAAACC GCGAG GUCA AGCAAAUCCC AUAAAGUUGU UCUCAGUUCG GAUUGUAGUC UGCAACUCGA CUACAUGAAG CUGGAAUCGC UAGUAA UCG UAGAUCAGCA UGCUACGGUG AAUACGUUCC CGGGUCUUGU ACACACCGCC CGUCACACCA CGAGAGUUUG UAACACC CG AAGCCGGUGG AGUAACCUUU UAGGAGCUAG CCGUCGAAGG UGGGACAAAU GAUUGGGGUG AAGUCGUAAC AAGGUAGC C GUAUCGGAAG GUGCGGCUGG AUCACCUCCU UUCU

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Macromolecule #23: 23S ribosomal RNA

MacromoleculeName: 23S ribosomal RNA / type: rna / ID: 23 / Number of copies: 2
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 946.697625 KDa
SequenceString: GAUUAAGUUA UUAAGGGCGC ACGGUGGAUG CCUUGGCACU AGAAGCCGAU GAAGGACGUU ACUAACGACG AUAUGCUUUG GGGAGCUGU AAGUAAGCUU UGAUCCAGAG AUUUCCGAAU GGGGAAACCC AGCAUGAGUU AUGUCAUGUU AUCGAUAUGU G AAUACAUA ...String:
GAUUAAGUUA UUAAGGGCGC ACGGUGGAUG CCUUGGCACU AGAAGCCGAU GAAGGACGUU ACUAACGACG AUAUGCUUUG GGGAGCUGU AAGUAAGCUU UGAUCCAGAG AUUUCCGAAU GGGGAAACCC AGCAUGAGUU AUGUCAUGUU AUCGAUAUGU G AAUACAUA GCAUAUCAGA AGGCACACCC GGAGAACUGA AACAUCUUAG UACCCGGAGG AAGAGAAAGA AAAUUCGAUU CC CUUAGUA GCGGCGAGCG AAAUGGGAAG AGCCCAAACC AACAAGCUUG CUUGUUGGGG UUGUAGGACA CUCUAUACGG AGU UACAAA GGACGACAUU AGACGAAUCA UCUGGAAAGA UGAAUCAAAG AAGGUAAUAA UCCUGUAGUC GAAAAUGUUG UCUC UCUUG AGUGGAUCCU GAGUACGACG GAGCACGUGA AAUUCCGUCG GAAUCUGGGA GGACCAUCUC CUAAGGCUAA AUACU CUCU AGUGACCGAU AGUGAACCAG UACCGUGAGG GAAAGGUGAA AAGCACCCCG GAAGGGGAGU GAAAUAGAAC CUGAAA CCG UGUGCUUACA AGUAGUCAGA GCCCGUUAAU GGGUGAUGGC GUGCCUUUUG UAGAAUGAAC CGGCGAGUUA CGAUUUG AU GCAAGGUUAA GCAGUAAAUG UGGAGCCGUA GCGAAAGCGA GUCUGAAUAG GGCGUUUAGU AUUUGGUCGU AGACCCGA A ACCAGGUGAU CUACCCUUGG UCAGGUUGAA GUUCAGGUAA CACUGAAUGG AGGACCGAAC CGACUUACGU UGAAAAGUG AGCGGAUGAA CUGAGGGUAG CGGAGAAAUU CCAAUCGAAC CUGGAGAUAG CUGGUUCUCU CCGAAAUAGC UUUAGGGCUA GCCUCAAGU GAUGAUUAUU GGAGGUAGAG CACUGUUUGG ACGAGGGGCC CCUCUCGGGU UACCGAAUUC AGACAAACUC C GAAUGCCA AUUAAUUUAA CUUGGGAGUC AGAACAUGGG UGAUAAGGUC CGUGUUCGAA AGGGAAACAG CCCAGACCAC CA GCUAAGG UCCCAAAAUA UAUGUUAAGU GGAAAAGGAU GUGGCGUUGC CCAGACAACU AGGAUGUUGG CUUAGAAGCA GCC AUCAUU UAAAGAGUGC GUAAUAGCUC ACUAGUCGAG UGACACUGCG CCGAAAAUGU ACCGGGGCUA AACAUAUUAC CGAA GCUGU GGAUUGUCCU UUGGACAAUG GUAGGAGAGC GUUCUAAGGG CGUUGAAGCA UGAUCGUAAG GACAUGUGGA GCGCU UAGA AGUGAGAAUG CCGGUGUGAG UAGCGAAAGA CGGGUGAGAA UCCCGUCCAC CGAUUGACUA AGGUUUCCAG AGGAAG GCU CGUCCGCUCU GGGUUAGUCG GGUCCUAAGC UGAGGCCGAC AGGCGUAGGC GAUGGAUAAC AGGUUGAUAU UCCUGUA CC ACCUAUAAUC GUUUUAAUCG AUGGGGGGAC GCAGUAGGAU AGGCGAAGCG UGCGAUUGGA UUGCACGUCU AAGCAGUA A GGCUGAGUAU UAGGCAAAUC CGGUACUCGU UAAGGCUGAG CUGUGAUGGG GAGAAGACAU UGUGUCUUCG AGUCGUUGA UUUCACACUG CCGAGAAAAG CCUCUAGAUA GAAAAUAGGU GCCCGUACCG CAAACCGACA CAGGUAGUCA AGAUGAGAAU UCUAAGGUG AGCGAGCGAA CUCUCGUUAA GGAACUCGGC AAAAUGACCC CGUAACUUCG GGAGAAGGGG UGCUCUUUAG G GUUAACGC CCAGAAGAGC CGCAGUGAAU AGGCCCAAGC GACUGUUUAU CAAAAACACA GGUCUCUGCU AAACCGUAAG GU GAUGUAU AGGGGCUGAC GCCUGCCCGG UGCUGGAAGG UUAAGAGGAG UGGUUAGCUU CUGCGAAGCU ACGAAUCGAA GCC CCAGUA AACGGCGGCC GUAACUAUAA CGGUCCUAAG GUAGCGAAAU UCCUUGUCGG GUAAGUUCCG ACCCGCACGA AAGG CGUAA CGAUUUGGGC ACUGUCUCAA CGAGAGACUC GGUGAAAUCA UAGUACCUGU GAAGAUGCAG GUUACCCGCG ACAGG ACGG AAAGACCCCG UGGAGCUUUA CUGUAGCCUG AUAUUGAAAU UCGGCACAGC UUGUACAGGA UAGGUAGGAG CCUUUG AAA CGUGAGCGCU AGCUUACGUG GAGGCGCUGG UGGGAUACUA CCCUAGCUGU GUUGGCUUUC UAACCCGCAC CACUUAU CG UGGUGGGAGA CAGUGUCAGG CGGGCAGUUU GACUGGGGCG GUCGCCUCCU AAAAGGUAAC GGAGGCGCUC AAAGGUUC C CUCAGAAUGG UUGGAAAUCA UUCAUAGAGU GUAAAGGCAU AAGGGAGCUU GACUGCGAGA CCUACAAGUC GAGCAGGGU CGAAAGACGG ACUUAGUGAU CCGGUGGUUC CGCAUGGAAG GGCCAUCGCU CAACGGAUAA AAGCUACCCC GGGGAUAACA GGCUUAUCU CCCCCAAGAG UUCACAUCGA CGGGGAGGUU UGGCACCUCG AUGUCGGCUC AUCGCAUCCU GGGGCUGUAG U CGGUCCCA AGGGUUGGGC UGUUCGCCCA UUAAAGCGGU ACGCGAGCUG GGUUCAGAAC GUCGUGAGAC AGUUCGGUCC CU AUCCGUC GUGGGCGUAG GAAAUUUGAG AGGAGCUGUC CUUAGUACGA GAGGACCGGG AUGGACAUAC CUCUGGUGUA CCA GUUGUC GUGCCAACGG CAUAGCUGGG UAGCUAUGUG UGGACGGGAU AAGUGCUGAA AGCAUCUAAG CAUGAAGCCC CCCU CAAGA UGAGAUUUCC CAACUUCGGU UAUAAGAUCC CUCAAAGAUG AUGAGGUUAA UAGGUUCGAG GUGGAAGCAU GGUGA CAUG UGGAGCUGAC GAAUACUAAU CGAUCGAAGA CUUAAUCAA

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Macromolecule #24: 5S ribosomal RNA

MacromoleculeName: 5S ribosomal RNA / type: rna / ID: 24 / Number of copies: 2
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 36.974945 KDa
SequenceString:
UCUGGUGACU AUAGCAAGGA GGUCACACCU GUUCCCAUGC CGAACACAGA AGUUAAGCUC CUUAGCGUCG AUGGUAGUCG AACUUACGU UCCGCUAGAG UAGAACGUUG CCAGGC

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Macromolecule #2: 30S ribosomal protein S2

MacromoleculeName: 30S ribosomal protein S2 / type: protein_or_peptide / ID: 2 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 25.929135 KDa
SequenceString: MAVISMKQLL EAGVHFGHQT RRWNPKMKKY IFTERNGIYI IDLQKTVKKV DEAYNFLKQV SEDGGQVLFV GTKKQAQESV KSEAERAGQ FYINQRWLGG LLTNYKTISK RIKRISEIEK MEEDGLFEVL PKKEVVELKK EYDRLIKFLG GIRDMKSMPQ A LFVVDPRK ...String:
MAVISMKQLL EAGVHFGHQT RRWNPKMKKY IFTERNGIYI IDLQKTVKKV DEAYNFLKQV SEDGGQVLFV GTKKQAQESV KSEAERAGQ FYINQRWLGG LLTNYKTISK RIKRISEIEK MEEDGLFEVL PKKEVVELKK EYDRLIKFLG GIRDMKSMPQ A LFVVDPRK ERNAIAEARK LNIPIVGIVD TNCDPDEIDY VIPANDDAIR AVKLLTAKMA DAILEGQQ

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Macromolecule #3: 30S ribosomal protein S3

MacromoleculeName: 30S ribosomal protein S3 / type: protein_or_peptide / ID: 3 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 22.684201 KDa
SequenceString: GQKINPIGLR VGIIRDWEAK WYAEKDFASL LHEDLKIRKF IDNELKEASV SHVEIERAAN RINIAIHTGK PGMVIGKGGS EIEKLRNKL NALTDKKVHI NVIEIKKVDL DARLVAENIA RQLENRASFR RVQKQAITRA MKLGAKGIKT QVSGRLGGAD I ARAEQYSE ...String:
GQKINPIGLR VGIIRDWEAK WYAEKDFASL LHEDLKIRKF IDNELKEASV SHVEIERAAN RINIAIHTGK PGMVIGKGGS EIEKLRNKL NALTDKKVHI NVIEIKKVDL DARLVAENIA RQLENRASFR RVQKQAITRA MKLGAKGIKT QVSGRLGGAD I ARAEQYSE GTVPLHTLRA DIDYAHAEAD TTYGKLGVKV WIYR

+
Macromolecule #4: 30S ribosomal protein S4

MacromoleculeName: 30S ribosomal protein S4 / type: protein_or_peptide / ID: 4 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 22.849145 KDa
SequenceString: RFRGSNWKKS RRLGISLSGT GKELEKRPYA PGQHGPNQRK KLSEYGLQLR EKQKLRYLYG MTERQFRNTF DIAGKKFGVH GENFMILLA SRLDAVVYSL GLARTRRQAR QLVNHGHILV DGKRVDIPSY SVKPGQTISV REKSQKLNII VESVEINNFV P EYLNFDAD ...String:
RFRGSNWKKS RRLGISLSGT GKELEKRPYA PGQHGPNQRK KLSEYGLQLR EKQKLRYLYG MTERQFRNTF DIAGKKFGVH GENFMILLA SRLDAVVYSL GLARTRRQAR QLVNHGHILV DGKRVDIPSY SVKPGQTISV REKSQKLNII VESVEINNFV P EYLNFDAD SLTGTFVRLP ERSELPAEIN EQLIVEYYSR

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Macromolecule #5: 30S ribosomal protein S5

MacromoleculeName: 30S ribosomal protein S5 / type: protein_or_peptide / ID: 5 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 16.522119 KDa
SequenceString:
EFEERVVTIN RVAKVVKGGR RFRFTALVVV GDKNGRVGFG TGKAQEVPEA IKKAVEAAKK DLVVVPRVEG TTPHTITGRY GSGSVFMKP AAPGTGVIAG GPVRAVLELA GITDILSKSL GSNTPINMVR ATIDGLQNLK NAEDVAKLRG KTVEELY

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Macromolecule #6: 30S ribosomal protein S6

MacromoleculeName: 30S ribosomal protein S6 / type: protein_or_peptide / ID: 6 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 11.239766 KDa
SequenceString:
MRTYEVMYIV RPNIEEDAKK ALVERFNGIL ATEGAEVLEA KDWGKRRLAY EINDFKDGFY NIVRVKSDNN KATDEFQRLA KISDDIIRY MVIRED

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Macromolecule #7: 30S ribosomal protein S7

MacromoleculeName: 30S ribosomal protein S7 / type: protein_or_peptide / ID: 7 / Details: Ribosomal protein uS7 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 17.18183 KDa
SequenceString:
MPRKGSVPKR DVLPDPIHNS KLVTKLINKI MLDGKRGTAQ RILYSAFDLV EQRSGRDALE VFEEAINNIM PVLEVKARRV GGSNYQVPV EVRPERRTTL GLRWLVNYAR LRGEKTMEDR LANEILDAAN NTGGAVKKRE DTHKMAEANK AFA

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Macromolecule #8: 30S ribosomal protein S8

MacromoleculeName: 30S ribosomal protein S8 / type: protein_or_peptide / ID: 8 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 14.723118 KDa
SequenceString:
TMTDPIADML TRVRNANMVR HEKLELPASN IKKEIAEILK SEGFIKNVEY VEDDKQGVLR LFLKYGQNDE RVITGLKRIS KPGLRVYAK ASEMPKVLNG LGIALVSTSE GVITDKEARK RNVGGEIIAY VW

+
Macromolecule #9: 30S ribosomal protein S9

MacromoleculeName: 30S ribosomal protein S9 / type: protein_or_peptide / ID: 9 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 14.312321 KDa
SequenceString:
VEYRGTGRRK NSVARVRLVP GEGNITVNNR DVREYLPFES LILDLNQPFD VTETKGNYDV LVNVHGGGFT GQAQAIRHGI ARALLEADP EYRGSLKRAG LLTRDPRMKE RKKPGLKAAR RSPQFSKR

+
Macromolecule #10: 30S ribosomal protein S10

MacromoleculeName: 30S ribosomal protein S10 / type: protein_or_peptide / ID: 10 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 11.009742 KDa
SequenceString:
IRIRLKAYDH RVIDQSAEKI VETAKRSGAD VSGPIPLPTE KSVYTIIRAV HKYKDSREQF EQRTHKRLID IVNPTPKTVD ALMGLNLPS GVDIEIKL

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Macromolecule #11: 30S ribosomal protein S11

MacromoleculeName: 30S ribosomal protein S11 / type: protein_or_peptide / ID: 11 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 12.047609 KDa
SequenceString:
NIENGVAHIR STFNNTIVTI TDEFGNALSW SSAGALGFKG SKKSTPFAAQ MASETASKSA MEHGLKTVEV TVKGPGPGRE SAIRALQSA GLEVTAIRDV TPVPHNGCRP PKRRR

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Macromolecule #12: 30S ribosomal protein S12

MacromoleculeName: 30S ribosomal protein S12 / type: protein_or_peptide / ID: 12 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 15.07557 KDa
SequenceString:
PTINQLVRKP RQSKIKKSDS PALNKGFNSK KKKFTDLNSP QKRGVCTRVG TMTPKKPNSA LRKYARVRLS NNIEINAYIP GIGHNLQEH SVVLVRGGRV KDLPGVRYHI VRGALDTSGV DGRRQGRSLY GTKKPK

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Macromolecule #13: 30S ribosomal protein S13

MacromoleculeName: 30S ribosomal protein S13 / type: protein_or_peptide / ID: 13 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 13.747919 KDa
SequenceString:
MARIAGVDIP REKRVVISLT YIYGIGTSTA QKILEEANVS ADTRVKDLTD DELGRIREVV DGYKVEGDLR RETNLNIKRL MEISSYRGI RHRRGLPVRG QKTKNNARTR KGPVKTVANK KK

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Macromolecule #14: 30S ribosomal protein S14 type Z

MacromoleculeName: 30S ribosomal protein S14 type Z / type: protein_or_peptide / ID: 14 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 7.186573 KDa
SequenceString:
AKTSMVAKQQ KKQKYAVREY TRCERCGRPH SVYRKFKLCR ICFRELAYKG QIPGVRKASW

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Macromolecule #15: 30S ribosomal protein S15

MacromoleculeName: 30S ribosomal protein S15 / type: protein_or_peptide / ID: 15 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 10.503135 KDa
SequenceString:
AISQERKNEI IKEYRVHETD TGSPEVQIAV LTAEINAVNE HLRTHKKDHH SRRGLLKMVG RRRHLLNYLR SKDIQRYREL IKSLGIRR

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Macromolecule #16: 30S ribosomal protein S16

MacromoleculeName: 30S ribosomal protein S16 / type: protein_or_peptide / ID: 16 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 9.99351 KDa
SequenceString:
AVKIRLTRLG SKRNPFYRIV VADARSPRDG RIIEQIGTYN PTSANAPEIK VDEALALKWL NDGAKPTDTV HNILSKEGIM KKFDEQKKA

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Macromolecule #17: 30S ribosomal protein S17

MacromoleculeName: 30S ribosomal protein S17 / type: protein_or_peptide / ID: 17 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 9.36686 KDa
SequenceString:
NDRKVYVGKV VSDKMDKTIT VLVETYKTHK LYGKRVKYSK KYKTHDENNS AKLGDIVKIQ ETRPLSATKR FRLVEIVEES

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Macromolecule #18: 30S ribosomal protein S18

MacromoleculeName: 30S ribosomal protein S18 / type: protein_or_peptide / ID: 18 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 6.404623 KDa
SequenceString:
THIDYKDTEL LKRFISERGK ILPRRVTGTS AKYQRMLTTA IKRSRHMALL PYVK

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Macromolecule #19: 30S ribosomal protein S19

MacromoleculeName: 30S ribosomal protein S19 / type: protein_or_peptide / ID: 19 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 9.205604 KDa
SequenceString:
SIKKGPFVDE HLMKKVEAQE GSEKKQVIKT WSRRSTIFPN FIGHTFAVYD GRKHVPVYVT EDMVGHKLGE FAPTRTFKGH

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Macromolecule #20: 30S ribosomal protein S20

MacromoleculeName: 30S ribosomal protein S20 / type: protein_or_peptide / ID: 20 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 8.779096 KDa
SequenceString:
ANIKSAIKRV KTTEKAEARN ISQKSAMRTA VKNAKTAVSN NADNKNELVS LAVKLVDKAA QSNLIHSNKA DRIKSQLMTA N

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Macromolecule #21: 30S ribosomal protein S21

MacromoleculeName: 30S ribosomal protein S21 / type: protein_or_peptide / ID: 21 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 6.24128 KDa
SequenceString:
TVVRKNESLE DALRRFKRSV SKSGTIQEVR KREFYEKPSV KRKKKSEAAR KR

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Macromolecule #22: Ribosome hibernation promotion factor

MacromoleculeName: Ribosome hibernation promotion factor / type: protein_or_peptide / ID: 22 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 22.244914 KDa
SequenceString:
MIRFEIHGDN LTITDAIRNY IEEKIGKLER YFNDVPNAVA HVKVKTYSNS ATKIEVTIPL KNVTLRAEER NDDLYAGIDL INNKLERQV RKYKTRINRK SRDRGDQEVF VAELQEMQET QVDNDAYDDN EIEIIRSKEF SLKPMDSEEA VLQMNLLGHD F FVFTDRET DGTSIVYRRK DGKYGLIQTS EQ

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Macromolecule #25: 50S ribosomal protein L2

MacromoleculeName: 50S ribosomal protein L2 / type: protein_or_peptide / ID: 25 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 29.827607 KDa
SequenceString: AIKKYKPITN GRRNMTSLDF AEITKTTPEK SLLKPLPKKA GRNNQGKLTV RHHGGGHKRQ YRVIDFKRNK DGINAKVDSI QYDPNRSAN IALVVYADGE KRYIIAPKGL EVGQIVESGA EADIKVGNAL PLQNIPVGTV VHNIELKPGK GGQIARSAGA S AQVLGKEG ...String:
AIKKYKPITN GRRNMTSLDF AEITKTTPEK SLLKPLPKKA GRNNQGKLTV RHHGGGHKRQ YRVIDFKRNK DGINAKVDSI QYDPNRSAN IALVVYADGE KRYIIAPKGL EVGQIVESGA EADIKVGNAL PLQNIPVGTV VHNIELKPGK GGQIARSAGA S AQVLGKEG KYVLIRLRSG EVRMILSTCR ATIGQVGNLQ HELVNVGKAG RSRWKGIRPT VRGSVMNPND HPHGGGEGRA PI GRPSPMS PWGKPTLGKK TRRGKKSSDK LIVRGRK

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Macromolecule #26: 50S ribosomal protein L3

MacromoleculeName: 50S ribosomal protein L3 / type: protein_or_peptide / ID: 26 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 23.199545 KDa
SequenceString: TKGILGRKIG MTQVFGENGE LIPVTVVEAK ENVVLQKKTV EVDGYNAIQV GFEDKKAYKK DAKSNKYANK PAEGHAKKAD AAPKRFIRE FRNVDVDAYE VGQEVSVDTF VAGDVIDVTG VSKGKGFQGA IKRHGQSRGP MSHGSHFHRA PGSVGMASDA S RVFKGQKM ...String:
TKGILGRKIG MTQVFGENGE LIPVTVVEAK ENVVLQKKTV EVDGYNAIQV GFEDKKAYKK DAKSNKYANK PAEGHAKKAD AAPKRFIRE FRNVDVDAYE VGQEVSVDTF VAGDVIDVTG VSKGKGFQGA IKRHGQSRGP MSHGSHFHRA PGSVGMASDA S RVFKGQKM PGRMGGNTVT VQNLEVVQVD TENKVILVKG NVPGPKKGLV EIRTSIK

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Macromolecule #27: 50S ribosomal protein L4

MacromoleculeName: 50S ribosomal protein L4 / type: protein_or_peptide / ID: 27 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 22.364504 KDa
SequenceString: ANYDVLKLDG TKSGSIELSD AVFGIEPNNS VLFEAINLQR ASLRQGTHAV KNRSAVSGGG RKPWKQKGTG RARQGTIRAP QWRGGGIVF GPTPRSYAYK MPKKMRRLAL RSALSFKAQE NGLTVVDAFN FEAPKTKEFK NVLSTLEQPK KVLVVTENED V NVELSARN ...String:
ANYDVLKLDG TKSGSIELSD AVFGIEPNNS VLFEAINLQR ASLRQGTHAV KNRSAVSGGG RKPWKQKGTG RARQGTIRAP QWRGGGIVF GPTPRSYAYK MPKKMRRLAL RSALSFKAQE NGLTVVDAFN FEAPKTKEFK NVLSTLEQPK KVLVVTENED V NVELSARN IPGVQVTTAQ GLNVLDITNA DSLVITEAAA KKVEEVLG

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Macromolecule #28: 50S ribosomal protein L5

MacromoleculeName: 50S ribosomal protein L5 / type: protein_or_peptide / ID: 28 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 19.731893 KDa
SequenceString:
NRLKEKFNTE VTENLMKKFN YSSVMEVPKI DKIVVNMGVG DAVQNSKVLD NAVEELELIT GQKPLVTKAK KSIATFRLRE GMPIGAKVT LRGERMYEFL DKLISVSLPR VRDFQGVSKK AFDGRGNYTL GVKEQLIFPE IDYDKVSKVR GMDIVIVTTA N TDEEAREL LANFGMP

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Macromolecule #29: 50S ribosomal protein L6

MacromoleculeName: 50S ribosomal protein L6 / type: protein_or_peptide / ID: 29 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 19.501154 KDa
SequenceString:
SRVGKKIIDI PSDVTVTFDG NHVTVKGPKG ELSRTLNERM TFKQEENTIE VVRPSDSKED RTNHGTTRAL LNNMVQGVSQ GYVKVLELV GVGYRAQMQG KDLILNVGYS HPVEIKAEEN ITFSVEKNTV VKVEGISKEQ VGALASNIRS VRPPEPYKGK G IRYQGEYV RRKEGKT

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Macromolecule #30: 50S ribosomal protein L13

MacromoleculeName: 50S ribosomal protein L13 / type: protein_or_peptide / ID: 30 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 16.359427 KDa
SequenceString:
MRQTFMANES NIERKWYVID AEGQTLGRLS SEVASILRGK NKVTYTPHVD TGDYVIVINA SKIEFTGNKE TDKVYYRHSN HPGGIKSIT AGELRRTNPE RLIENSIKGM LPSTRLGEKQ GKKLFVYGGA EHPHAAQQPE NYELRG

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Macromolecule #31: 50S ribosomal protein L14

MacromoleculeName: 50S ribosomal protein L14 / type: protein_or_peptide / ID: 31 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 13.157342 KDa
SequenceString:
MIQQETRLKV ADNSGAREVL TIKVLGGSGR KTANIGDVIV CTVKNATPGG VVKKGDVVKA VIVRTKSGVR RNDGSYIKFD ENACVIIRD DKGPRGTRIF GPVARELREG NFMKIVSLAP EVL

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Macromolecule #32: 50S ribosomal protein L15

MacromoleculeName: 50S ribosomal protein L15 / type: protein_or_peptide / ID: 32 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 15.62889 KDa
SequenceString:
MKLHELKPAE GSRKERNRVG RGVATGNGKT SGRGHKGQKA RSGGGVRPGF EGGQLPLFRR LPKRGFTNIN RKEYAIVNLD QLNKFEDGT EVTPALLVES GVVKNEKSGI KILGNGSLDK KLTVKAHKFS ASAAEAIDAK GGAHEVI

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Macromolecule #33: 50S ribosomal protein L16

MacromoleculeName: 50S ribosomal protein L16 / type: protein_or_peptide / ID: 33 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 15.599435 KDa
SequenceString:
MLLPKRVKYR RQHRPKTTGR SKGGNYVTFG EFGLQATTTS WITSRQIESA RIAMTRYMKR GGKVWIKIFP HTPYTKKPLE VRMGAGKGA VEGWIAVVKP GRILFEVAGV SEEVAREALR LASHKLPVKT KFVKREEL

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Macromolecule #34: 50S ribosomal protein L17

MacromoleculeName: 50S ribosomal protein L17 / type: protein_or_peptide / ID: 34 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 13.583523 KDa
SequenceString:
YRKLGRTSDQ RKAMLRDLAT SLIISERIET TEARAKEVRS VVEKLITLGK KGDLASRRNA AKTLRNVEIL NEDETTQTAL QKLFGEIAE RYTERQGGYT RILKQGPRRG DGAESVIIEL V

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Macromolecule #35: 50S ribosomal protein L18

MacromoleculeName: 50S ribosomal protein L18 / type: protein_or_peptide / ID: 35 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 13.124093 KDa
SequenceString:
MISKIDKNKV RLKRHARVRT NLSGTAEKPR LNVYRSNKHI YAQIIDDNKG VTLAQASSKD SDIATTATKV ELATKVGEAI AKKAADKGI KEIVFDRGGY LYHGRVKALA EAARESGLEF

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Macromolecule #36: 50S ribosomal protein L19

MacromoleculeName: 50S ribosomal protein L19 / type: protein_or_peptide / ID: 36 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 13.104381 KDa
SequenceString:
TNHKLIEAVT KSQLRTDLPS FRPGDTLRVH VRIIEGTRER IQVFEGVVIK RRGGGVSETF TVRKISSGVG VERTFPLHTP KIEKIEVKR RGKVRRAKLY YLRSLRGKAA RIQEI

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Macromolecule #37: 50S ribosomal protein L20

MacromoleculeName: 50S ribosomal protein L20 / type: protein_or_peptide / ID: 37 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 13.459919 KDa
SequenceString:
PRVKGGTVTR ARRKKTIKLA KGYFGSKHTL YKVAKQQVMK SGQYAFRDRR QRKRDFRKLW ITRINAAARQ HEMSYSRLMN GLKKAGIDI NRKMLSEIAI SDEKAFAQLV TKAKDAL

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Macromolecule #38: 50S ribosomal protein L21

MacromoleculeName: 50S ribosomal protein L21 / type: protein_or_peptide / ID: 38 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 11.354081 KDa
SequenceString:
MFAIIETGGK QIKVEEGQEI FVEKLDVNEG DTFTFDKVLF VGGDSVKVGA PTVEGATVTA TVNKQGRGKK ITVFTYKRRK NSKRKKGHR QPYTKLTIDK INA

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Macromolecule #39: 50S ribosomal protein L22

MacromoleculeName: 50S ribosomal protein L22 / type: protein_or_peptide / ID: 39 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 12.328358 KDa
SequenceString:
MEAKAVARTI RIAPRKVRLV LDLIRGKNAA EAIAILKLTN KASSPVIEKV LMSALANAEH NYDMNTDELV VKEAYANEGP TLKRFRPRA QGRASAINKR TSHITIVVSD GKE

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Macromolecule #40: 50S ribosomal protein L23

MacromoleculeName: 50S ribosomal protein L23 / type: protein_or_peptide / ID: 40 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 10.3801 KDa
SequenceString:
EARDILKRPV ITEKSSEAMA EDKYTFDVDT RVNKTQVKMA VEEIFNVKVA SVNIMNYKPK KKRMGRYQGY TNKRRKAIVT LKEGSIDLF

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Macromolecule #41: 50S ribosomal protein L24

MacromoleculeName: 50S ribosomal protein L24 / type: protein_or_peptide / ID: 41 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 11.316205 KDa
SequenceString:
HIKKGDNVKV IAGKDKGKEG KVIATLPKKD RVVVEGVNIM KKHQKPTQLN PEGGILETEA AIHVSNVQLL DPKTNEPTRV GYKFVDGKK VRIAKKSGEE IKSN

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Macromolecule #42: 50S ribosomal protein L25

MacromoleculeName: 50S ribosomal protein L25 / type: protein_or_peptide / ID: 42 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 10.4622 KDa
SequenceString:
ASLKSIIRQG KQTRSDLKQL RKSGKVPAVV YGYGTKNVSV KVDEVEFIKV IREVGRNGVI ELGVGSKTIK VMVADYQFDP LKNQITHID FLAIN

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Macromolecule #43: 50S ribosomal protein L27

MacromoleculeName: 50S ribosomal protein L27 / type: protein_or_peptide / ID: 43 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 8.911099 KDa
SequenceString:
KKGVSSTKNG RDSESKRLGA KRADGQFVTG GSILYRQRGT KIYPGENVGR GGDDTLFAKI DGVVKFERKG RDKKQVSVYA VA

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Macromolecule #44: 50S ribosomal protein L28

MacromoleculeName: 50S ribosomal protein L28 / type: protein_or_peptide / ID: 44 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 6.550716 KDa
SequenceString:
KQCFVTGRKA STGNRRSHAL NSTKRRWNAN LQKVRILVDG KPKKVWVSAR ALKSGKVT

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Macromolecule #45: 50S ribosomal protein L29

MacromoleculeName: 50S ribosomal protein L29 / type: protein_or_peptide / ID: 45 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 7.845942 KDa
SequenceString:
KAKEIRDLTT SEIEEQIKSS KEELFNLRFQ LATGQLEETA RIRTVRKTIA RLKTVARERE IEQSKAN

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Macromolecule #46: 50S ribosomal protein L30

MacromoleculeName: 50S ribosomal protein L30 / type: protein_or_peptide / ID: 46 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 6.434487 KDa
SequenceString:
AKLQITLTRS VIGRPETQRK TVEALGLKKT NSSVVVEDNP AIRGQINKVK HLVTVEEK

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Macromolecule #47: 50S ribosomal protein L31 type B

MacromoleculeName: 50S ribosomal protein L31 type B / type: protein_or_peptide / ID: 47 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 6.909694 KDa
SequenceString:
MKQGIHPEYH QVIFLDTTTN FKFLSGSTKT SSEMMEWEDG KEYPVIRLDI SSDSHPFYT

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Macromolecule #48: 50S ribosomal protein L32

MacromoleculeName: 50S ribosomal protein L32 / type: protein_or_peptide / ID: 48 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 5.569565 KDa
SequenceString:
AVPKRRTSKT RKNKRRTHFK ISVPGMTECP NCGEYKLSHR VCKNCGSY

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Macromolecule #49: 50S ribosomal protein L33

MacromoleculeName: 50S ribosomal protein L33 / type: protein_or_peptide / ID: 49 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 5.684561 KDa
SequenceString:
RVNVTLACTE CGDRNYITTK NKRNNPERIE MKKYCPRLNK YTLHRET

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Macromolecule #50: 50S ribosomal protein L34

MacromoleculeName: 50S ribosomal protein L34 / type: protein_or_peptide / ID: 50 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 5.252369 KDa
SequenceString:
VKRTYQPNKR KHSKVHGFRK RMSTKNGRKV LARRRRKGRK VLS

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Macromolecule #51: 50S ribosomal protein L35

MacromoleculeName: 50S ribosomal protein L35 / type: protein_or_peptide / ID: 51 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 7.461992 KDa
SequenceString:
PKMKTHRGAA KRVKRTASGQ LKRSRAFTSH LFANKSTKQK RQLRKARLVS KSDMKRVKQL LAYK

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Macromolecule #52: 50S ribosomal protein L36

MacromoleculeName: 50S ribosomal protein L36 / type: protein_or_peptide / ID: 52 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 4.318422 KDa
SequenceString:
MKVRPSVKPI CEKCKVIKRK GKVMVICENP KHKQRQG

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 7.5
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Image recordingFilm or detector model: FEI FALCON II (4k x 4k) / Average electron dose: 2.3 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Final reconstructionNumber classes used: 1 / Resolution.type: BY AUTHOR / Resolution: 6.76 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 12570
Initial angle assignmentType: OTHER
Final angle assignmentType: OTHER

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