+
Open data
-
Basic information
Entry | ![]() | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
Title | Filament structure of GAC with phosphate | |||||||||
![]() | ||||||||||
![]() |
| |||||||||
![]() | Glutaminase / phosphate / filament / PROTEIN FIBRIL / HYDROLASE | |||||||||
Function / homology | ![]() glutamine catabolic process / regulation of respiratory gaseous exchange by nervous system process / glutamate biosynthetic process / Glutamate and glutamine metabolism / intracellular glutamate homeostasis / Glutamate Neurotransmitter Release Cycle / glutaminase / glutaminase activity / suckling behavior / TP53 Regulates Metabolic Genes ...glutamine catabolic process / regulation of respiratory gaseous exchange by nervous system process / glutamate biosynthetic process / Glutamate and glutamine metabolism / intracellular glutamate homeostasis / Glutamate Neurotransmitter Release Cycle / glutaminase / glutaminase activity / suckling behavior / TP53 Regulates Metabolic Genes / chemical synaptic transmission / protein homotetramerization / mitochondrial matrix / synapse / mitochondrion / cytosol Similarity search - Function | |||||||||
Biological species | ![]() | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 2.9 Å | |||||||||
![]() | Guo CJ / Liu JL | |||||||||
Funding support | ![]()
| |||||||||
![]() | ![]() Title: Structural basis for activation and filamentation of glutaminase. Authors: Chen-Jun Guo / Zi-Xuan Wang / Ji-Long Liu / ![]() ![]() | |||||||||
History |
|
-
Structure visualization
Supplemental images |
---|
-
Downloads & links
-EMDB archive
Map data | ![]() | 20.6 MB | ![]() | |
---|---|---|---|---|
Header (meta data) | ![]() ![]() | 13.5 KB 13.5 KB | Display Display | ![]() |
FSC (resolution estimation) | ![]() | 7.1 KB | Display | ![]() |
Images | ![]() | 34.6 KB | ||
Masks | ![]() | 30.5 MB | ![]() | |
Filedesc metadata | ![]() | 5.3 KB | ||
Others | ![]() ![]() | 22.5 MB 22.5 MB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Validation report
Summary document | ![]() | 768.8 KB | Display | ![]() |
---|---|---|---|---|
Full document | ![]() | 768.4 KB | Display | |
Data in XML | ![]() | 12.8 KB | Display | |
Data in CIF | ![]() | 17.7 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 8imaMC ![]() 8imbC M: atomic model generated by this map C: citing same article ( |
---|---|
Similar structure data | Similarity search - Function & homology ![]() |
-
Links
EMDB pages | ![]() ![]() |
---|---|
Related items in Molecule of the Month |
-
Map
File | ![]() | ||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Voxel size | X=Y=Z: 1.06 Å | ||||||||||||||||||||
Density |
| ||||||||||||||||||||
Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
|
-Supplemental data
-Mask #1
File | ![]() | ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Projections & Slices |
| ||||||||||||
Density Histograms |
-Half map: #1
File | emd_35573_half_map_1.map | ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Projections & Slices |
| ||||||||||||
Density Histograms |
-Half map: #2
File | emd_35573_half_map_2.map | ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Projections & Slices |
| ||||||||||||
Density Histograms |
-
Sample components
-Entire : GAC filament with phosphate
Entire | Name: GAC filament with phosphate |
---|---|
Components |
|
-Supramolecule #1: GAC filament with phosphate
Supramolecule | Name: GAC filament with phosphate / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1 |
---|---|
Source (natural) | Organism: ![]() |
-Macromolecule #1: Glutaminase kidney isoform, mitochondrial
Macromolecule | Name: Glutaminase kidney isoform, mitochondrial / type: protein_or_peptide / ID: 1 / Number of copies: 4 / Enantiomer: LEVO / EC number: glutaminase |
---|---|
Source (natural) | Organism: ![]() |
Molecular weight | Theoretical: 53.083738 KDa |
Recombinant expression | Organism: ![]() ![]() |
Sequence | String: LVASGENKIK QGLLPSLEDL LFYTIAEGQE KIPVHKFITA LKSTGLRTSD PRLKECMDML RLTLQTTSDG VMLDKDLFKK CVQSNIVLL TQAFRRKFVI PDFMSFTSHI DELYESAKKQ SGGKVADYIP QLAKFSPDLW GVSVCTVDGQ RHSTGDTKVP F CLQSCVKP ...String: LVASGENKIK QGLLPSLEDL LFYTIAEGQE KIPVHKFITA LKSTGLRTSD PRLKECMDML RLTLQTTSDG VMLDKDLFKK CVQSNIVLL TQAFRRKFVI PDFMSFTSHI DELYESAKKQ SGGKVADYIP QLAKFSPDLW GVSVCTVDGQ RHSTGDTKVP F CLQSCVKP LKYAIAVNDL GTEYVHRYVG KEPSGLRFNK LFLNEDDKPH NPMVNAGAIV VTSLIKQGVN NAEKFDYVMQ FL NKMAGNE YVGFSNATFQ SERESGDRNF AIGYYLKEKK CFPEGTDMVG ILDFYFQLCS IEVTCESASV MAATLANGGF CPI TGERVL SPEAVRNTLS LMHSCGMYDF SGQFAFHVGL PAKSGVAGGI LLVVPNVMGM MCWSPPLDKM GNSVKGIHFC HDLV SLCNF HNYDNLRHFA KKLDPRREGG DQRHSFGPLD YESLQQELAL KETVWKKVSP ESNEDISTTV VYRMESLGEK S UniProtKB: Glutaminase kidney isoform, mitochondrial |
-Macromolecule #2: PHOSPHATE ION
Macromolecule | Name: PHOSPHATE ION / type: ligand / ID: 2 / Number of copies: 4 / Formula: PO4 |
---|---|
Molecular weight | Theoretical: 94.971 Da |
Chemical component information | ![]() ChemComp-PO4: |
-Experimental details
-Structure determination
Method | cryo EM |
---|---|
![]() | single particle reconstruction |
Aggregation state | filament |
-
Sample preparation
Buffer | pH: 8.5 |
---|---|
Vitrification | Cryogen name: ETHANE |
-
Electron microscopy
Microscope | FEI TITAN KRIOS |
---|---|
Image recording | Film or detector model: GATAN K3 (6k x 4k) / Average electron dose: 50.0 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 1.8 µm / Nominal defocus min: 1.2 µm |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |