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Open data
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Basic information
Entry | ![]() | |||||||||||||||
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Title | F1 domain of FoF1-ATPase from Bacillus PS3,post-hyd',lowATP | |||||||||||||||
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![]() | ATP synthase F1 ATPase FoF1 / MOTOR PROTEIN | |||||||||||||||
Function / homology | ![]() proton motive force-driven plasma membrane ATP synthesis / proton-transporting ATP synthase complex, catalytic core F(1) / H+-transporting two-sector ATPase / proton-transporting ATPase activity, rotational mechanism / proton-transporting ATP synthase activity, rotational mechanism / ADP binding / ATP hydrolysis activity / ATP binding / plasma membrane Similarity search - Function | |||||||||||||||
Biological species | ![]() ![]() | |||||||||||||||
Method | single particle reconstruction / cryo EM / Resolution: 3.3 Å | |||||||||||||||
![]() | Nakano A / Kishikawa J / Mitsuoka K / Yokoyama K | |||||||||||||||
Funding support | ![]()
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![]() | ![]() Title: Rotation mechanism of ATP synthases driven by ATP hydrolysis Authors: Nakano A / Yokoyama K / Kishikawa J / Mitsuoka K | |||||||||||||||
History |
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Structure visualization
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 140.5 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 21.2 KB 21.2 KB | Display Display | ![]() |
FSC (resolution estimation) | ![]() | 12.8 KB | Display | ![]() |
Images | ![]() | 76.5 KB | ||
Masks | ![]() | 178 MB | ![]() | |
Filedesc metadata | ![]() | 6.5 KB | ||
Others | ![]() ![]() ![]() | 166.3 MB 140.9 MB 140.9 MB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 8hhcMC ![]() 8hh1C ![]() 8hh2C ![]() 8hh3C ![]() 8hh4C ![]() 8hh5C ![]() 8hh6C ![]() 8hh7C ![]() 8hh8C ![]() 8hh9C ![]() 8hhaC ![]() 8hhbC M: atomic model generated by this map C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
EMDB pages | ![]() ![]() |
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Related items in Molecule of the Month |
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Map
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Voxel size | X=Y=Z: 0.88 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Mask #1
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-Additional map: #1
File | emd_34760_additional_1.map | ||||||||||||
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Density Histograms |
-Half map: #2
File | emd_34760_half_map_1.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Half map: #1
File | emd_34760_half_map_2.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
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Sample components
-Entire : FoF1 from Bacillus PS3
Entire | Name: FoF1 from Bacillus PS3 |
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Components |
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-Supramolecule #1: FoF1 from Bacillus PS3
Supramolecule | Name: FoF1 from Bacillus PS3 / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1 |
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Source (natural) | Organism: ![]() ![]() |
Molecular weight | Theoretical: 530 KDa |
-Macromolecule #1: ATP synthase subunit alpha
Macromolecule | Name: ATP synthase subunit alpha / type: protein_or_peptide / ID: 1 / Number of copies: 3 / Enantiomer: LEVO / EC number: H+-transporting two-sector ATPase |
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Source (natural) | Organism: ![]() ![]() |
Molecular weight | Theoretical: 54.717398 KDa |
Recombinant expression | Organism: ![]() ![]() |
Sequence | String: SIRAEEISAL IKQQIENYES QIQVSDVGTV IQVGDGIARA HGLDNVMSGE LVEFANGVMG MALNLEENNV GIVILGPYTG IKEGDEVRR TGRIMEVPVG EALIGRVVNP LGQPVDGLGP VETTETRPIE SPAPGVMDRR SVHEPLQTGI KAIDALVPIG R GQRELIIG ...String: SIRAEEISAL IKQQIENYES QIQVSDVGTV IQVGDGIARA HGLDNVMSGE LVEFANGVMG MALNLEENNV GIVILGPYTG IKEGDEVRR TGRIMEVPVG EALIGRVVNP LGQPVDGLGP VETTETRPIE SPAPGVMDRR SVHEPLQTGI KAIDALVPIG R GQRELIIG DRQTGKTSVA IDTIINQKDQ NMISIYVAIG QKESTVRTVV ETLRKHGALD YTIVVTASAS QPAPLLFLAP YA GVAMGEY FMYKGKHVLV VYDDLSKQAA AYRELSLLLR RPPGREAYPG DIFYLHSRLL ERAAKLSDAK GGGSLTALPF VET QAGDIS AYIPTNVISI TDGQIFLQSD LFFSGVRPAI NAGLSVSRVG GAAQIKAMKK VAGTLRLDLA AYRELEAFAQ FGSD LDKAT QAKLARGART VEVLKQDLHQ PIPVEKQVLI IYALTRGFLD DIPVEDVRRF EKEFYLFLDQ NGQHLLEHIR TTKDL PNED DLNKAIEAFK KTFVVSQ UniProtKB: ATP synthase subunit alpha |
-Macromolecule #2: ATP synthase subunit beta
Macromolecule | Name: ATP synthase subunit beta / type: protein_or_peptide / ID: 2 / Number of copies: 3 / Enantiomer: LEVO / EC number: H+-transporting two-sector ATPase |
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Source (natural) | Organism: ![]() ![]() |
Molecular weight | Theoretical: 53.424625 KDa |
Recombinant expression | Organism: ![]() ![]() |
Sequence | String: MHHHHHHHHH HMTRGRVIQV MGPVVDVKFE NGHLPAIYNA LKIQHKARNE NEVDIDLTLE VALHLGDDTV RTIAMASTDG LIRGMEVID TGAPISVPVG EVTLGRVFNV LGEPIDLEGD IPADARRDPI HRPAPKFEEL ATEVEILETG IKVVDLLAPY I KGGKIGLF ...String: MHHHHHHHHH HMTRGRVIQV MGPVVDVKFE NGHLPAIYNA LKIQHKARNE NEVDIDLTLE VALHLGDDTV RTIAMASTDG LIRGMEVID TGAPISVPVG EVTLGRVFNV LGEPIDLEGD IPADARRDPI HRPAPKFEEL ATEVEILETG IKVVDLLAPY I KGGKIGLF GGAGVGKTVL IQELIHNIAQ EHGGISVFAG VGERTREGND LYHEMKDSGV ISKTAMVFGQ MNEPPGARMR VA LTGLTMA EYFRDEQGQD VLLFIDNIFR FTQAGSEVSA LLGRMPSAVG YQPTLATEMG QLQERITSTA KGSITSIQAI YVP ADDYTD PAPATTFSHL DATTNLERKL AEMGIYPAVD PLASTSRALA PEIVGEEHYQ VARKVQQTLQ RYKELQDIIA ILGM DELSD EDKLVVHRAR RIQFFLSQNF HVAEQFTGQP GSYVPVKETV RGFKEILEGK YDHLPEDAFR LVGRIEEVVE KAKAM GVEV UniProtKB: ATP synthase subunit beta |
-Macromolecule #3: ATP synthase gamma chain
Macromolecule | Name: ATP synthase gamma chain / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO |
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Source (natural) | Organism: ![]() ![]() |
Molecular weight | Theoretical: 31.728328 KDa |
Recombinant expression | Organism: ![]() ![]() |
Sequence | String: ASLRDIKTRI NATKKTSQIT KAMEMVSTSK LNRAEQNAKS FVPYMEKIQE VVANVALGAG GASHPMLVSR PVKKTGYLVI TSDRGLAGA YNSNVLRLVY QTIQKRHASP DEYAIIVIGR VGLSFFRKRN MPVILDITRL PDQPSFADIK EIARKTVGLF A DGTFDELY ...String: ASLRDIKTRI NATKKTSQIT KAMEMVSTSK LNRAEQNAKS FVPYMEKIQE VVANVALGAG GASHPMLVSR PVKKTGYLVI TSDRGLAGA YNSNVLRLVY QTIQKRHASP DEYAIIVIGR VGLSFFRKRN MPVILDITRL PDQPSFADIK EIARKTVGLF A DGTFDELY MYYNHYVSAI QQEVTERKLL PLTDLAENKQ RTVYEFEPSQ EEILDVLLPQ YAESLIYGAL LDAKASEHAA RM TAMKNAT DNANELIRTL TLSYNRARQA AITQEITEIV AGANALQ UniProtKB: ATP synthase gamma chain |
-Macromolecule #4: ADENOSINE-5'-TRIPHOSPHATE
Macromolecule | Name: ADENOSINE-5'-TRIPHOSPHATE / type: ligand / ID: 4 / Number of copies: 5 / Formula: ATP |
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Molecular weight | Theoretical: 507.181 Da |
Chemical component information | ![]() ChemComp-ATP: |
-Macromolecule #5: MAGNESIUM ION
Macromolecule | Name: MAGNESIUM ION / type: ligand / ID: 5 / Number of copies: 5 / Formula: MG |
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Molecular weight | Theoretical: 24.305 Da |
-Macromolecule #6: PHOSPHATE ION
Macromolecule | Name: PHOSPHATE ION / type: ligand / ID: 6 / Number of copies: 1 / Formula: PO4 |
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Molecular weight | Theoretical: 94.971 Da |
Chemical component information | ![]() ChemComp-PO4: |
-Experimental details
-Structure determination
Method | cryo EM |
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![]() | single particle reconstruction |
Aggregation state | particle |
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Sample preparation
Concentration | 10 mg/mL |
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Buffer | pH: 8 |
Vitrification | Cryogen name: ETHANE |
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Electron microscopy
Microscope | FEI TITAN KRIOS |
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Image recording | Film or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Number grids imaged: 1 / Number real images: 7653 / Average electron dose: 60.0 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Cs: 0.3 mm / Nominal defocus max: 2.0 µm / Nominal defocus min: 0.8 µm |
Sample stage | Cooling holder cryogen: NITROGEN |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |