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- EMDB-31393: Structure of the phycobilisome from the red alga Porphyridium pur... -

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Open data

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Basic information

Entry

Database: EMDB / ID: EMD-31393

Title

Structure of the phycobilisome from the red alga Porphyridium purpureum in Middle Light

Map data

Sample

Complex: phycobilisome
- Protein or peptide: x 28 types
Ligand: x 4 types

Keywords

light-harvesting complex /

photosynthesis

Function / homology

Function and homology information

phycobilisome / chloroplast thylakoid membrane /

photosynthesis /

lyase activity
Similarity search - Function

Biological species

Porphyridium purpureum (eukaryote)

Method

single particle reconstruction /

cryo EM / Resolution: 3.0 Å

Authors

Ma JF / Sui S-F

Funding support

China, 2 items

Citation

Journal: Commun Biol / Year: 2023
Title: The structural basis for light acclimation in phycobilisome light harvesting systems systems in Porphyridium purpureum
Authors: Dodson EJ / Ma J / Suissa Szlejf M / Maroudas-Sklare N / Paltiel Y / Adir N / Sun S / Sui SF / Keren N

History

Deposition	Jun 2, 2021	-
Header (metadata) release	Sep 28, 2022	-
Map release	Sep 28, 2022	-
Update	Apr 3, 2024	-
Current status	Apr 3, 2024	Processing site: PDBj / Status: Released

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Structure visualization

Supplemental images	emd_31393.png

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Downloads & links

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EMDB archive

Map data	emd_31393.map.gz	92 MB		EMDB map data format
Header (meta data)	emd-31393-v30.xml emd-31393.xml	52.5 KB 52.5 KB	Display Display	EMDB header
Images	emd_31393.png	123.8 KB
Filedesc metadata	emd-31393.cif.gz	12.4 KB
Archive directory	http://ftp.pdbj.org/pub/emdb/structures/EMD-31393 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-31393	HTTPS FTP

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Related structure data

Related structure data	7ezxMC 31397C 31406C 31407C 31408C 31409C 31410C 31411C 31412C 31413C 31414C 31415C 31416C 31417C 31418C 34534 C: citing same article (ref.) M: atomic model generated by this map
Similar structure data	Similarity search - Function & homologyF&H Search Similarity search - FunctionF&H Search Similarity search - HomologyF&H Search

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Links

EMDB pages	EMDB (EBI/PDBe) / EMDataResource

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Map

File

Download / File: emd_31393.map.gz / Format: CCP4 / Size: 744.3 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)

Voxel size

X=Y=Z: 1.066 Å

Density

Contour Level	By AUTHOR: 0.02
Minimum - Maximum	-0.06504417 - 0.121381335
Average (Standard dev.)	0.000544629 (±0.004794267)

Symmetry

Space group: 1

Details

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Supplemental data

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Sample components

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Entire : phycobilisome

Entire	Name: phycobilisome
Components	Complex: phycobilisome Protein or peptide: Phycobilisome 31.8 kDa linker polypeptide, phycoerythrin-associated, rod Protein or peptide: R-phycoerythrin gamma chain, chloroplastic Protein or peptide: Phycobilisome rod-core linker polypeptide Protein or peptide: C-phycocyanin alpha subunit Protein or peptide: C-phycocyanin beta subunit Protein or peptide: Phycoerythrin alpha subunit Protein or peptide: B-phycoerythrin beta chain Protein or peptide: Phycobilisome 31.8 kDa linker polypeptide, phycoerythrin-associated, rod Protein or peptide: Phycobilisome 31.8 kDa linker polypeptide, phycoerythrin-associated, rod Protein or peptide: Phycobilisome 27.9 kDa linker polypeptide, phycoerythrin-associated, rod Protein or peptide: CaRSPs2 Protein or peptide: CaRSPs1 Protein or peptide: FAS1 domain-containing protein Protein or peptide: R-phycoerythrin gamma chain, chloroplastic Protein or peptide: Phycobilisome 32.1 kDa linker polypeptide, phycocyanin-associated, rod Protein or peptide: R-phycoerythrin gamma chain, chloroplastic Protein or peptide: R-phycoerythrin gamma chain, chloroplastic Protein or peptide: Phycobilisome 27.9 kDa linker polypeptide, phycoerythrin-associated, rod Protein or peptide: Phycobilisome 31.8 kDa linker polypeptide, phycoerythrin-associated, rod Protein or peptide: Allophycocyanin alpha subunit Protein or peptide: Allophycocyanin beta subunit Protein or peptide: Allophycocyanin gamma subunit Protein or peptide: Allophycocyanin beta 18 subunit Protein or peptide: Phycobilisome 7.8 kDa linker polypeptide, allophycocyanin-associated, core Protein or peptide: Phycobilisome linker polypeptide Protein or peptide: Lrc4 Protein or peptide: LRC5 Protein or peptide: FAS1 domain-containing protein Ligand: PHYCOERYTHROBILIN Ligand: PHYCOUROBILIN Ligand: PHYCOCYANOBILIN Ligand: phenylmethanesulfonic acid

Entire

Name: phycobilisome

Components

Complex: phycobilisome
- Protein or peptide: Phycobilisome 31.8 kDa linker polypeptide, phycoerythrin-associated, rod
- Protein or peptide: R-phycoerythrin gamma chain, chloroplastic
- Protein or peptide: Phycobilisome rod-core linker polypeptide
- Protein or peptide: C-phycocyanin alpha subunit
- Protein or peptide: C-phycocyanin beta subunit
- Protein or peptide: Phycoerythrin alpha subunit
- Protein or peptide: B-phycoerythrin beta chain
- Protein or peptide: Phycobilisome 31.8 kDa linker polypeptide, phycoerythrin-associated, rod
- Protein or peptide: Phycobilisome 31.8 kDa linker polypeptide, phycoerythrin-associated, rod
- Protein or peptide: Phycobilisome 27.9 kDa linker polypeptide, phycoerythrin-associated, rod
- Protein or peptide: CaRSPs2
- Protein or peptide: CaRSPs1
- Protein or peptide: FAS1 domain-containing protein
- Protein or peptide: R-phycoerythrin gamma chain, chloroplastic
- Protein or peptide: Phycobilisome 32.1 kDa linker polypeptide, phycocyanin-associated, rod
- Protein or peptide: R-phycoerythrin gamma chain, chloroplastic
- Protein or peptide: R-phycoerythrin gamma chain, chloroplastic
- Protein or peptide: Phycobilisome 27.9 kDa linker polypeptide, phycoerythrin-associated, rod
- Protein or peptide: Phycobilisome 31.8 kDa linker polypeptide, phycoerythrin-associated, rod
- Protein or peptide: Allophycocyanin alpha subunit
- Protein or peptide: Allophycocyanin beta subunit
- Protein or peptide: Allophycocyanin gamma subunit
- Protein or peptide: Allophycocyanin beta 18 subunit
- Protein or peptide: Phycobilisome 7.8 kDa linker polypeptide, allophycocyanin-associated, core
- Protein or peptide: Phycobilisome linker polypeptide
- Protein or peptide: Lrc4
- Protein or peptide: LRC5
- Protein or peptide: FAS1 domain-containing protein
Ligand: PHYCOERYTHROBILIN
Ligand: PHYCOUROBILIN
Ligand: PHYCOCYANOBILIN
Ligand: phenylmethanesulfonic acid

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Supramolecule #1: phycobilisome

Supramolecule	Name: phycobilisome / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#28
Source (natural)	Organism: Porphyridium purpureum (eukaryote)
Molecular weight	Theoretical: 14700 kDa/nm

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Macromolecule #1: Phycobilisome 31.8 kDa linker polypeptide, phycoerythrin-associat...

Macromolecule	Name: Phycobilisome 31.8 kDa linker polypeptide, phycoerythrin-associated, rod type: protein_or_peptide / ID: 1 / Number of copies: 6 / Enantiomer: LEVO
Source (natural)	Organism: Porphyridium purpureum (eukaryote)
Molecular weight	Theoretical: 39.743625 KDa
Sequence	String: MAAFVTGMGA APRSQMRSVS GSAVCVRSDS VRAPGALTMR LGTVSGNSSL DKLGLDKFLS ESNRAYTPRA QPGFSSEYEQ IISATYKQL FGNAYIMDSE RAEMAKQESM FRDGQLTLKD FCRALAKTEQ YKKRFFDSRP LYGAIELNFK NILGRTPDGL E HYRAKSAV ...String: MAAFVTGMGA APRSQMRSVS GSAVCVRSDS VRAPGALTMR LGTVSGNSSL DKLGLDKFLS ESNRAYTPRA QPGFSSEYEQ IISATYKQL FGNAYIMDSE RAEMAKQESM FRDGQLTLKD FCRALAKTEQ YKKRFFDSRP LYGAIELNFK NILGRTPDGL E HYRAKSAV YDTKGYEAFV DAFFDDGEYD EVYDDYTVPF YRGYKTEANL SMAAFTHFFR MVRGSSTSDK ANPNSMQKDI PL NYYGITK TPLAVIAPGA AGTAYTESFS GTGSWQSGRA GLNAARVALG VPATANGKSF RVEVTGYTQP GFGITAGTAV GKL YKANKL SRYPRSNKSY VVGFDELTPL YQRITKNGGT IASITPL UniProtKB: Phycobilisome 31.8 kDa linker polypeptide, phycoerythrin-associated, rod

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Macromolecule #2: R-phycoerythrin gamma chain, chloroplastic

Macromolecule	Name: R-phycoerythrin gamma chain, chloroplastic / type: protein_or_peptide / ID: 2 / Number of copies: 6 / Enantiomer: LEVO
Source (natural)	Organism: Porphyridium purpureum (eukaryote)
Molecular weight	Theoretical: 31.792982 KDa
Sequence	String: MAAFVSGFHG VQVGAPAENK LVCRAAKPAQ LTMLTGYDSK SSPNFPNRAA TRERRTVSFN ARVARNKSQA KKILEKADEF FARSVTMQY KAFACPNGVY DIQCTEGTVK GAAYEKRAMA VSAAFRAKQA SPAAKARALF ENRRHAIIAS HECQHEEDLF V RFPKLSAA ...String: MAAFVSGFHG VQVGAPAENK LVCRAAKPAQ LTMLTGYDSK SSPNFPNRAA TRERRTVSFN ARVARNKSQA KKILEKADEF FARSVTMQY KAFACPNGVY DIQCTEGTVK GAAYEKRAMA VSAAFRAKQA SPAAKARALF ENRRHAIIAS HECQHEEDLF V RFPKLSAA YMMGKTEAMR TCSRYVVPDS LEEEYMAASV DRQMKERACP GGVYASSCVE GNAKGQAEQA RVAALATAFR SA QKSASKT TAERYSSAAY GRDHFAHGCS YEESVFNTYP ATAAAMRSKS YNY UniProtKB: R-phycoerythrin gamma chain, chloroplastic

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Macromolecule #3: Phycobilisome rod-core linker polypeptide

Macromolecule	Name: Phycobilisome rod-core linker polypeptide / type: protein_or_peptide / ID: 3 / Number of copies: 6 / Enantiomer: LEVO
Source (natural)	Organism: Porphyridium purpureum (eukaryote)
Molecular weight	Theoretical: 26.996689 KDa
Sequence	String: MSIPILKYSL STQNQRVESF EVNPGDEQYR IYSTDSLPSS SEVDQIIWAA YRQIFSEHQI LKSTKQPFLE SQLRFNQISI KDFIKGLVL SAPFRDLNYN VNNNYRFVEI CVQRVLGRDI YNEREKIAWS IIIATQGLEG FIDTLLNTQE YLSNFGDSIV P FQRRRIIP ...String: MSIPILKYSL STQNQRVESF EVNPGDEQYR IYSTDSLPSS SEVDQIIWAA YRQIFSEHQI LKSTKQPFLE SQLRFNQISI KDFIKGLVL SAPFRDLNYN VNNNYRFVEI CVQRVLGRDI YNEREKIAWS IIIATQGLEG FIDTLLNTQE YLSNFGDSIV P FQRRRIIP QRSKGEMPFN LKTPRYGIEF RSKLAATQFS WASPIRRFRP QEQQPKAGDP ALFLNMVQEM GPGL UniProtKB: Phycobilisome rod-core linker polypeptide

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Macromolecule #4: C-phycocyanin alpha subunit

Macromolecule	Name: C-phycocyanin alpha subunit / type: protein_or_peptide / ID: 4 / Number of copies: 36 / Enantiomer: LEVO
Source (natural)	Organism: Porphyridium purpureum (eukaryote)
Molecular weight	Theoretical: 17.478664 KDa
Sequence	String: MKTPITEAIA TADNQGRFLS NTELQAVNGR YQRATASLTA AKALTGSAQR LITGAAQAVY NKFPYTTQMP GPAYASSAIG KAKCARDIG YYLRMVTYCL VVGGTGPMDE YLVAGLEEIN RSFDLSPSWY IEALQYIKNS HGLSGQVANE ANAYIDYAIN T LS UniProtKB: C-phycocyanin alpha subunit

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Macromolecule #5: C-phycocyanin beta subunit

Macromolecule	Name: C-phycocyanin beta subunit / type: protein_or_peptide / ID: 5 / Number of copies: 36 / Enantiomer: LEVO
Source (natural)	Organism: Porphyridium purpureum (eukaryote)
Molecular weight	Theoretical: 18.242705 KDa
Sequence	String: MLDAFAKVVA QADARGEFLS NTQIDALSKM VKEGNQRLDI VNKVTSNASA IVTNSARALF AEQPQLIQPG G(MEN)AYTS RRM AACLRDMEIV LRYVSYAMIA GDSSVLDDRC LNGLRETYQA LGTPGSSVAV AIQKMKDASV ALANDTTGTP IGDCSSL VA ELAGYFDRAA VSVV UniProtKB: C-phycocyanin beta subunit

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Macromolecule #6: Phycoerythrin alpha subunit

Macromolecule	Name: Phycoerythrin alpha subunit / type: protein_or_peptide / ID: 6 / Number of copies: 230 / Enantiomer: LEVO
Source (natural)	Organism: Porphyridium purpureum (eukaryote)
Molecular weight	Theoretical: 17.824029 KDa
Sequence	String: MKSVITTVVS AADAAGRFPS NSDLESIQGN IQRSAARLEA AEKLAGNHEA VVKEAGDACF AKYAYLKNPG EAGENQEKIN KCYRDVDHY MRLVNYCLVV GGTGPLDEWG IAGAREVYRT LNLPTSAYVA SIAYTRDRLC VPRDMSAQAG VEFSAYLDYL I NALS UniProtKB: Phycoerythrin alpha subunit

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Macromolecule #7: B-phycoerythrin beta chain

Macromolecule	Name: B-phycoerythrin beta chain / type: protein_or_peptide / ID: 7 / Number of copies: 250 / Enantiomer: LEVO
Source (natural)	Organism: Porphyridium purpureum (eukaryote)
Molecular weight	Theoretical: 18.584182 KDa
Sequence	String: MLDAFSRVVV NSDAKAAYVG GSDLQALKSF IADGNKRLDA VNSIVSNASC MVSDAVSGMI CENPGLISPG G(MEN)CYTN RRM AACLRDGEII LRYVSYALLA GDASVLEDRC LNGLKETYIA LGVPTNSSIR AVSIMKAQAV AFITNTATER KMSFAAG DC TSLASEVASY FDRVGAAIS UniProtKB: B-phycoerythrin beta chain

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Macromolecule #8: Phycobilisome 31.8 kDa linker polypeptide, phycoerythrin-associat...

Macromolecule	Name: Phycobilisome 31.8 kDa linker polypeptide, phycoerythrin-associated, rod type: protein_or_peptide / ID: 8 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)	Organism: Porphyridium purpureum (eukaryote)
Molecular weight	Theoretical: 32.886242 KDa
Sequence	String: MEAVGFVHVG AWRVGSSSAA AAAAGVAGKR CGALPVSFGA HAPRRAASAS QVKMMAFSSI QNVLTDAPLG TPSAMGFLDE RTEKPLMGP SEMDELVYAV YKQVFGNAHI MESERAELRD AESLFRYLRD VREFVRALAK SNAYTSRFLQ GNQQYRFFEL N FKHLLGRG ...String: MEAVGFVHVG AWRVGSSSAA AAAAGVAGKR CGALPVSFGA HAPRRAASAS QVKMMAFSSI QNVLTDAPLG TPSAMGFLDE RTEKPLMGP SEMDELVYAV YKQVFGNAHI MESERAELRD AESLFRYLRD VREFVRALAK SNAYTSRFLQ GNQQYRFFEL N FKHLLGRG PRSLEEMASH SALYAEGGFE AVIDAILDSD EYAQEFGSTV VPYCRFKGEY PTNEEFNRML VLKGANSTSD KA GKGRSKL CYSIAAGCSQ NWLSMSKALP FGTEKGTGFN LISSSRNPAA RPRIGTKIPG GVVFSS UniProtKB: Phycobilisome 31.8 kDa linker polypeptide, phycoerythrin-associated, rod

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Macromolecule #9: Phycobilisome 31.8 kDa linker polypeptide, phycoerythrin-associat...

Macromolecule	Name: Phycobilisome 31.8 kDa linker polypeptide, phycoerythrin-associated, rod type: protein_or_peptide / ID: 9 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)	Organism: Porphyridium purpureum (eukaryote)
Molecular weight	Theoretical: 43.968605 KDa
Sequence	String: MAAFISSFAG AQLAHARPSK QTALRAALTL PGTAETNLAV PSNAVRKVQP YPIAKPPSYS SVDSLRPARV SRMDADWATI YEQVRRQVM GNAYVMEGEA PDIDVAFSQL KGGNLTVREF VRAVGKSASY RTRFMEAKSS YNFVLLNFKH FLGRAPTQEE V STHIQILA ...String: MAAFISSFAG AQLAHARPSK QTALRAALTL PGTAETNLAV PSNAVRKVQP YPIAKPPSYS SVDSLRPARV SRMDADWATI YEQVRRQVM GNAYVMEGEA PDIDVAFSQL KGGNLTVREF VRAVGKSASY RTRFMEAKSS YNFVLLNFKH FLGRAPTQEE V STHIQILA TSGLEAEIDS YIDSDEYKAL FGDHVVPYVV YRGTYLSSER FNRMVKANPG GATSDKAKSN LNMIATVAAD LP TDAIDVM RGLPSPITSE TLAFGTAYYW AKVEKEASEG RSASPIGEKI GKFDHAPIST YTSLCSYDKV NKAPQISVTN VGS DEHSYV SVTSKYIAPD MAAAAQMLAD CQKYKAGGNA PTGKWMKYYP GTTVNMAPYI SLNDTGSDSS RTVSVTLDKV KISP NKIGK UniProtKB: Phycobilisome 31.8 kDa linker polypeptide, phycoerythrin-associated, rod

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Macromolecule #10: Phycobilisome 27.9 kDa linker polypeptide, phycoerythrin-associat...

Macromolecule	Name: Phycobilisome 27.9 kDa linker polypeptide, phycoerythrin-associated, rod type: protein_or_peptide / ID: 10 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)	Organism: Porphyridium purpureum (eukaryote)
Molecular weight	Theoretical: 37.175957 KDa
Sequence	String: MAFVTGGVGA VVRPGTAVSR GVVCARQQAR PRAAALQMGV SDVSITLSGV AASQPVSAPA KMSLEDRQLL VLQAIKQVFG NAYVMEEER ASFAKQESMF LSGELSVREF VRELALSDTY RRRFFEPCGP YRFVELNMKH LLGRGPISQA EVSQHVQCYV N NGYEAEIS ...String: MAFVTGGVGA VVRPGTAVSR GVVCARQQAR PRAAALQMGV SDVSITLSGV AASQPVSAPA KMSLEDRQLL VLQAIKQVFG NAYVMEEER ASFAKQESMF LSGELSVREF VRELALSDTY RRRFFEPCGP YRFVELNMKH LLGRGPISQA EVSQHVQCYV N NGYEAEIS SYVDSDEYYE RFGEDTVPYE QFRGTYMTAE DFNRMVSMYG APGQSDKSLT SRARSTGVAN SNKVLSLEGA GR SSKTVGR VATNTASSLT SVKSGIPPRP DIDQPRGQSS KRLVGRRLEI VPGSYMYLSP AEAAEYRAQQ AAVSQVSAAF SAD VQSKMA QVSKLKRELA ALGLSV UniProtKB: Phycobilisome 27.9 kDa linker polypeptide, phycoerythrin-associated, rod

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Macromolecule #11: CaRSPs2

Macromolecule	Name: CaRSPs2 / type: protein_or_peptide / ID: 11 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)	Organism: Porphyridium purpureum (eukaryote)
Molecular weight	Theoretical: 36.402004 KDa
Sequence	String: MWEQQRPRRC EAPAAPSSRP AERRAAARRS RAQLRMKQDD YEQWKTEFAG GFPGGEAFYK KWIEEGAKGD VPALEEELQP RSPNKKPTI YEEQMISNRG QQKGVDPTWK TLLAGGFPGG EFFFKKWIGE GAQGEVPNLD ADLQPGSGSA KKTGKKEDAD K SSPGGIMT ...String: MWEQQRPRRC EAPAAPSSRP AERRAAARRS RAQLRMKQDD YEQWKTEFAG GFPGGEAFYK KWIEEGAKGD VPALEEELQP RSPNKKPTI YEEQMISNRG QQKGVDPTWK TLLAGGFPGG EFFFKKWIGE GAQGEVPNLD ADLQPGSGSA KKTGKKEDAD K SSPGGIMT PGRIMVPSGL GEEEETVDKE APRPELYNKY FSADRLHKAP EILFEYNKTK YDRVGVRYTE VTSKASERFF PK SRMNRAP VIEISYREGA VSTASVSLSM PEISGPPALP FPVPKGDVTT TMVTDPTTGR LKLEFKVDGA AVSAYSDPRA TEA YNKMIR K UniProtKB: Uncharacterized protein

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Macromolecule #12: CaRSPs1

Macromolecule	Name: CaRSPs1 / type: protein_or_peptide / ID: 12 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)	Organism: Porphyridium purpureum (eukaryote)
Molecular weight	Theoretical: 31.172643 KDa
Sequence	String: MAAFVSGGCG VGGQRRAWPA KGAAVARTHA CPTTMVVLPV ARAGLAATAK KNQYMGTSVA PEIVLTDKGS DMSRKVKTED KKVAADQAA AMGILANMSL YASLNPVKRM TYKAKEQAPA YVKKTGNPVE DFYPSSWRNM APVISLSANR VAVAFEKIDA A SNGVKANS ...String: MAAFVSGGCG VGGQRRAWPA KGAAVARTHA CPTTMVVLPV ARAGLAATAK KNQYMGTSVA PEIVLTDKGS DMSRKVKTED KKVAADQAA AMGILANMSL YASLNPVKRM TYKAKEQAPA YVKKTGNPVE DFYPSSWRNM APVISLSANR VAVAFEKIDA A SNGVKANS NNKPFWKSGA TTKNYVAPEA PAQSEPETLD DAAYQRYFPA RIRNKAPAME FRRPSFANTE DPSAYFMLQK ET VPLRMAL AEKLLTKLGR KGDGSEAEEE KVKPQKKAAK KDAKDDAKDD E UniProtKB: Uncharacterized protein

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Macromolecule #13: FAS1 domain-containing protein

Macromolecule	Name: FAS1 domain-containing protein / type: protein_or_peptide / ID: 13 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)	Organism: Porphyridium purpureum (eukaryote)
Molecular weight	Theoretical: 31.332955 KDa
Sequence	String: MAAKPYESGY IAEDDFWRGR GIAAWVYATG ANKVIAQIVK DFNLTDKKFM VFIPNDGAFA RLSPQLRKAM MEDSRLVYDM LAGHIFTSK GSAMLKDLQG AGYLQPAYGE AIGYVGTGRV IKIGNAQVIP ESSDILRKNL GFSAHTLDTF IVPKALTKKV S IEAGFSPV ...String: MAAKPYESGY IAEDDFWRGR GIAAWVYATG ANKVIAQIVK DFNLTDKKFM VFIPNDGAFA RLSPQLRKAM MEDSRLVYDM LAGHIFTSK GSAMLKDLQG AGYLQPAYGE AIGYVGTGRV IKIGNAQVIP ESSDILRKNL GFSAHTLDTF IVPKALTKKV S IEAGFSPV TPAKYVSTTK ADLRYVGATK PAAVGGRRAM NLMKQQPFWM YGPPYNAVTQ DEYEPISAAA PKAFVDYQIF AP GTVKVSP DSVNANELNP VSGMSKYIGK TQKLVGDQGI SDRSDKLPMG Q UniProtKB: FAS1 domain-containing protein

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Macromolecule #14: R-phycoerythrin gamma chain, chloroplastic

Macromolecule	Name: R-phycoerythrin gamma chain, chloroplastic / type: protein_or_peptide / ID: 14 / Number of copies: 4 / Enantiomer: LEVO
Source (natural)	Organism: Porphyridium purpureum (eukaryote)
Molecular weight	Theoretical: 36.924992 KDa
Sequence	String: MAAFVVGAQV SSGFMGCAAP RKEQTRVGGA AKAAVSMRII IQDKNSYRKY QTNTSTSKWD KLLNTKPMKR QVQPNPPTNE TRALNLGNT FRSPAFKFLG TLKRSKDPSG LRLGFYGRKA DDFMARSIAM QAKASAAGSG VYTTQCSEGA SKGMAENART A SLAKQFRQ ...String: MAAFVVGAQV SSGFMGCAAP RKEQTRVGGA AKAAVSMRII IQDKNSYRKY QTNTSTSKWD KLLNTKPMKR QVQPNPPTNE TRALNLGNT FRSPAFKFLG TLKRSKDPSG LRLGFYGRKA DDFMARSIAM QAKASAAGSG VYTTQCSEGA SKGMAENART A SLAKQFRQ AQRSAREMSF DYYEGRKYAM KAVGHICNYE EKIFQQYNKT AAAYVMGKQE TLLSCDRYAQ PANKAEEYIQ KS VQMQMKK RSIPYGVYTT SCADGTVKGM AENARVAKES ANFRARQMSA GAKAAARFNA RRVANDWHNN GCNYEEKLTS RFP AAASSV RPTTNRY UniProtKB: R-phycoerythrin gamma chain, chloroplastic

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Macromolecule #15: Phycobilisome 32.1 kDa linker polypeptide, phycocyanin-associated, rod

Macromolecule	Name: Phycobilisome 32.1 kDa linker polypeptide, phycocyanin-associated, rod type: protein_or_peptide / ID: 15 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)	Organism: Porphyridium purpureum (eukaryote)
Molecular weight	Theoretical: 53.601336 KDa
Sequence	String: MAFVNGFSGG AAAVPRRGVA RVCAQRVVMK VVDRLDSQPS AAFEQTKQVY TFSRYILGPH RAVVAPVAMD PSEKEVVLRA VYRQVFGNA YIMEEERAEL RVMESQFLLG ELSVKELVRA LAKSSTYKVR FFEGAVQYRF IELCFKHLLG RAPDNHEEIA V HMRKYQQE ...String: MAFVNGFSGG AAAVPRRGVA RVCAQRVVMK VVDRLDSQPS AAFEQTKQVY TFSRYILGPH RAVVAPVAMD PSEKEVVLRA VYRQVFGNA YIMEEERAEL RVMESQFLLG ELSVKELVRA LAKSSTYKVR FFEGAVQYRF IELCFKHLLG RAPDNHEEIA V HMRKYQQE GYDAEIDSYL DAGEYDNVFG DDTVPFLRFR GVYTPCDSFN RQCALQGGWA NSDKAMGGAA LSGYNGSDGR QM STMIGNY ISGKPIPYEK VAADTPLKST APNWYARPNP ALAPQPAYVS AKEIAELRSR VSKLEAAWSV AVKQSAAAKD TVE TWRAAA KEMAAMRGIS PMGEAYFGGI AQKVDNGALA QLGNKASSYK KYLYAIETDE VSRLEVDLEE AKGQLRVLEA AMAK STPMT RTAEFKTLTK NVAAVTAAEK ADPLSKRPRI SASRPKPTPA AAKMAAAEKK KGPFGLSVPS LPNLPNLPKL PSLSL PKVS LPFGKK UniProtKB: Phycobilisome 32.1 kDa linker polypeptide, phycocyanin-associated, rod

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Macromolecule #16: R-phycoerythrin gamma chain, chloroplastic

Macromolecule	Name: R-phycoerythrin gamma chain, chloroplastic / type: protein_or_peptide / ID: 16 / Number of copies: 6 / Enantiomer: LEVO
Source (natural)	Organism: Porphyridium purpureum (eukaryote)
Molecular weight	Theoretical: 29.453639 KDa
Sequence	String: MAAFVGSAAS AFTGASAVKA NEKRSVCSLQ MVAMPQTGLV NSKFSARMAK KTAKQTKNKV DEYMARSVQR QYKQAAVATG VYGTQCTEG TVKGAAEASR SAALSRQFRI KQRSAFSKAH DLFEFRKHAI IAAAGCSYEE KMVTRFPKLA AAMVLGQTEM M RTCSRYVV ...String: MAAFVGSAAS AFTGASAVKA NEKRSVCSLQ MVAMPQTGLV NSKFSARMAK KTAKQTKNKV DEYMARSVQR QYKQAAVATG VYGTQCTEG TVKGAAEASR SAALSRQFRI KQRSAFSKAH DLFEFRKHAI IAAAGCSYEE KMVTRFPKLA AAMVLGQTEM M RTCSRYVV PESVEEEYMA ASVDKQMKRR GAPGGVYSLS CAEGVAKGQA EIARVSALGA AYRAASKSAS AVTAERYNSM AY GRVHFAH GCSYEEQQFN KYPAAAAAMR SDSYGY UniProtKB: R-phycoerythrin gamma chain, chloroplastic

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Macromolecule #17: R-phycoerythrin gamma chain, chloroplastic

Macromolecule	Name: R-phycoerythrin gamma chain, chloroplastic / type: protein_or_peptide / ID: 17 / Number of copies: 4 / Enantiomer: LEVO
Source (natural)	Organism: Porphyridium purpureum (eukaryote)
Molecular weight	Theoretical: 31.358787 KDa
Sequence	String: METAFVSGFM GKAAVAKFGA TAVCDKTARR SSSSNSQVHM VTGAVSSVNM RRFQRVPKVS GFSAKVTKKN VNKALDKADM FFAKSVTME GKAAAIPYGV YGIQCMEGSA KGMAHEKRAM ALSAAFRMNQ RSAAEKTGAM YENRRLALIL AQNDHQEKQY I KYPKLAAA ...String: METAFVSGFM GKAAVAKFGA TAVCDKTARR SSSSNSQVHM VTGAVSSVNM RRFQRVPKVS GFSAKVTKKN VNKALDKADM FFAKSVTME GKAAAIPYGV YGIQCMEGSA KGMAHEKRAM ALSAAFRMNQ RSAAEKTGAM YENRRLALIL AQNDHQEKQY I KYPKLAAA ALMASTEVTR ACQRYAVPES IEEEFLAASV DKVNKMRGTT ASGVYKSSCV EGNAKGQAEQ ARVAALAVAF RS AQKSASQ FAAERYAQSK YGRDLFSSTH FEEGYANTYP AMAAAKRASS YGY UniProtKB: R-phycoerythrin gamma chain, chloroplastic

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Macromolecule #18: Phycobilisome 27.9 kDa linker polypeptide, phycoerythrin-associat...

Macromolecule	Name: Phycobilisome 27.9 kDa linker polypeptide, phycoerythrin-associated, rod type: protein_or_peptide / ID: 18 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)	Organism: Porphyridium purpureum (eukaryote)
Molecular weight	Theoretical: 34.958746 KDa
Sequence	String: MAFVSGAGAA VRGTSGKAAA RCAAVRTVRA ARAIRMTTAP GSAPFSSLDE PERQVVMWES VGDEKDQVFK QLYRQVFGNA YLMESDLEE LLVPESQLLM GSISVKDFIK RVAKSDAYKK RFFEPCGPYR FVELCTKHFL GRGPRDQKEV SEHVQRLANE G YDADVDSY ...String: MAFVSGAGAA VRGTSGKAAA RCAAVRTVRA ARAIRMTTAP GSAPFSSLDE PERQVVMWES VGDEKDQVFK QLYRQVFGNA YLMESDLEE LLVPESQLLM GSISVKDFIK RVAKSDAYKK RFFEPCGPYR FVELCTKHFL GRGPRDQKEV SEHVQRLANE G YDADVDSY MDSEEYMSLF GENGVPRFVF KGTYEGNDQF NRLAAMRQFA DGSYTDTRSG STAPRKAQKA ELTMAEGDFV GR AKVSRGL PAETSAAKTG TPPVRALKGP VNPRAGVRVR IKVVDNLYQV YEIPPMADPK AKVNAFWAKP IPSSLTKKY UniProtKB: Phycobilisome 27.9 kDa linker polypeptide, phycoerythrin-associated, rod

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Macromolecule #19: Phycobilisome 31.8 kDa linker polypeptide, phycoerythrin-associat...

Macromolecule	Name: Phycobilisome 31.8 kDa linker polypeptide, phycoerythrin-associated, rod type: protein_or_peptide / ID: 19 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)	Organism: Porphyridium purpureum (eukaryote)
Molecular weight	Theoretical: 55.146727 KDa
Sequence	String: MAFVGAMPGG GGEPRAVRMM MQTDARSLSS GSSAAVPFST AVRFESPSGG LDRYSRVDPA APGPNVITRF LFKDRPVRRS DPSLSEVDR EATMRTVYRN VMGNAYVMEE ERAELATLES QFLVGAISTR DFVRGVAKSA TYKKRFFESV SQFRFIELNF K HFMGRAPL ...String: MAFVGAMPGG GGEPRAVRMM MQTDARSLSS GSSAAVPFST AVRFESPSGG LDRYSRVDPA APGPNVITRF LFKDRPVRRS DPSLSEVDR EATMRTVYRN VMGNAYVMEE ERAELATLES QFLVGAISTR DFVRGVAKSA TYKKRFFESV SQFRFIELNF K HFMGRAPL DMAEMSKHYE IFAAGGYDAE VDSYFDSEEY LDVFGLDTVP YMRFRGTYAP NSTFNLQCRL QGGWARSDKK LP MMSMLPL NNKAAIMPHQ IVDGLPVIPN SEHPSQKYNV PKVSREKLQR ELLIAQGKAN ALQIELDAAY TSLASSRAFL APF AAMAAD MDIRPLYGKN PQVFAGQFLG VGAGQWGKTG ADTVRGRSRR VAADIGVKEF QLERVKQLVV DLQRALALED AEAD APATS LLQAYQAKVY VKPPVIAKKK GPEPVNEDEI TIGQGDKKIK VTVLRNLGDR TEKLREKPEK EEEEGPRTFK DLYET AKPM KGFPGDGSEM NVGG UniProtKB: Phycobilisome 31.8 kDa linker polypeptide, phycoerythrin-associated, rod

+
Macromolecule #20: Allophycocyanin alpha subunit

Macromolecule	Name: Allophycocyanin alpha subunit / type: protein_or_peptide / ID: 20 / Number of copies: 20 / Enantiomer: LEVO
Source (natural)	Organism: Porphyridium purpureum (eukaryote)
Molecular weight	Theoretical: 17.639123 KDa
Sequence	String: MSIVTKSIVN ADAEARYLSP GELDRIKSFV LSGQRRLRIA QTLTENRERI VKQGGQQLFQ RRPDVVSPGG NAYGEEMTAT CLRDLDYYL RLVTYGIIAG DVTPIEEIGL VGVKEMYSAL GTPISGVAEG IRCMKDVACS LLSGEDAAEV GFYFDYTLAA M Q UniProtKB: Allophycocyanin alpha subunit

+
Macromolecule #21: Allophycocyanin beta subunit

Macromolecule	Name: Allophycocyanin beta subunit / type: protein_or_peptide / ID: 21 / Number of copies: 22 / Enantiomer: LEVO
Source (natural)	Organism: Porphyridium purpureum (eukaryote)
Molecular weight	Theoretical: 17.415797 KDa
Sequence	String: MQDAITSVIN AADVQGKYLD ANSVEKLRGY FQTGELRVRA AATIAANAAT IIKEAVAKSL LYSDITRPGG (MEN)MYTTR RYA ACIRDLDYYL RYATYGMLAG DPSILDERVL NGLKETYNSL GVPIGATIQA IQAMKEVTGS LVGSDAGKEM GLYFDYI CS GLS UniProtKB: Allophycocyanin beta subunit

+
Macromolecule #22: Allophycocyanin gamma subunit

Macromolecule	Name: Allophycocyanin gamma subunit / type: protein_or_peptide / ID: 22 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)	Organism: Porphyridium purpureum (eukaryote)
Molecular weight	Theoretical: 18.126934 KDa
Sequence	String: MSLVTQVILS ADDELRYPTA GELETISSYL KTGEYRIRLI SILQGKEQEI IRLASKKIFQ LHPEYIAPGG NASGARQRAL CLRDYGWYL RLITYAILAG DKEPLEKIGI IGVREMYNSL GVPIIGMIDA IKCLKEATVE VISQEEEDFV APYYDYIIQG M S UniProtKB: Allophycocyanin gamma subunit

+
Macromolecule #23: Allophycocyanin beta 18 subunit

Macromolecule	Name: Allophycocyanin beta 18 subunit / type: protein_or_peptide / ID: 23 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)	Organism: Porphyridium purpureum (eukaryote)
Molecular weight	Theoretical: 19.77874 KDa
Sequence	String: MQDIITSVIN QYDLSGRYLD IKGINQINNY FDTGLKRLMI AKLINKEATN LIKEASELLF IEQPELLRPC GNAFIGNAYT TRRYAACIR DIEYYLRYSA YSIIAGNNSI LDERVLDGLK ETYNSLLVPI GPTIRVIQLL KEIIQKKFST ENIENAIIAE P FDYLAINL SDKNL UniProtKB: Allophycocyanin beta 18 subunit

+
Macromolecule #24: Phycobilisome 7.8 kDa linker polypeptide, allophycocyanin-associa...

Macromolecule	Name: Phycobilisome 7.8 kDa linker polypeptide, allophycocyanin-associated, core type: protein_or_peptide / ID: 24 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)	Organism: Porphyridium purpureum (eukaryote)
Molecular weight	Theoretical: 14.108206 KDa
Sequence	String: MAGGAAFVLT SSGFAGTRLR SAAVCGAQGK SRAHGVAVAM AYDMDGTGAG SLEPRGIKSS AVEKDSRCFK VTFVLPSQSR LYTFRELQN VYTTKMVPFS SWYAEQQRIQ KMGGKILKVE LVAGGQMRSV GNQ UniProtKB: Phycobilisome 7.8 kDa linker polypeptide, allophycocyanin-associated, core

+
Macromolecule #25: Phycobilisome linker polypeptide

Macromolecule	Name: Phycobilisome linker polypeptide / type: protein_or_peptide / ID: 25 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)	Organism: Porphyridium purpureum (eukaryote)
Molecular weight	Theoretical: 99.468367 KDa
Sequence	String: MVTKASGGSP VVKPQLYKTA SMLTIAQAEQ QDRFLELGEL NQLVSFLNTG NIRLEIADLL TKNANIIVAR AADRIFVGGS AISYLERPQ ASIIEANSAD IASIRQMSGD SQSNFLENLT SVFQGNNATP TGFKPISVVR YGPSRMKKSL RDLDWFLRYL T YAIVAGDP ...String: MVTKASGGSP VVKPQLYKTA SMLTIAQAEQ QDRFLELGEL NQLVSFLNTG NIRLEIADLL TKNANIIVAR AADRIFVGGS AISYLERPQ ASIIEANSAD IASIRQMSGD SQSNFLENLT SVFQGNNATP TGFKPISVVR YGPSRMKKSL RDLDWFLRYL T YAIVAGDP NILFVNIRGL REIIENACSS AATIVALKEM KKTSLSLFPE NSIQKEIIEE YFNVVVDEFI NPALTDTIRK RT SNDLQGL RLPQIYAKAG ISRQKFVMKP GLSTDEKQSV ISACYRQVFE RDISKAYGFS FSVLESQVKN GQISIKEFVR SLG KSSVYQ KQFYQPYVNS RVVELAFRHF LGRNLSSLAE FQKFFAILSK KGLTGLVDSL INSREYSDYF NEETVPYIRG FGEE PQECR NWGTQIDLFQ YSAPFRKVPQ SITLFSDYLK ALPDQHPYGR GNDPLLIQFG AIFPIGTKNL KQNPAPFGKD TRRLL IRRG PGIYNQVGNP STRSVSVGSL GPKVFKSEGI NSNAQRTNNE SILQASYLAV FGRMIYQNER IGLKGIDNKF LDNNLS VKE LIRSLAISDT FRSLYWTPLY VCKSIEWIHY RLLGRPTYGR QEINQYFNVA YKKGFVGVIN SIIDSVEYNE CFGDNIV PY ERYLTANSVS QRQLKLGNII KSANLKPQNI EKFVQLGQSQ TNQNLYSIKY KVKQGVSKLR DQQKIFETKG SLSKDAYL S IFQAACRQIF ERDISTFVIG NEIENIKIQF IKGQISVKEM INALGKSSVY LKEFYNPYPN IKVIELGTKH FLGRAPNNQ AEIRFYNQIL ASCGLQAFID MLTNSQEYAE IFGEVRVPFR RFPTLPAANF PNTNTLFDKQ TKQNSVVIVP SFKAITGNQ UniProtKB: Phycobiliprotein ApcE

+
Macromolecule #26: Lrc4

Macromolecule	Name: Lrc4 / type: protein_or_peptide / ID: 26 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)	Organism: Porphyridium purpureum (eukaryote)
Molecular weight	Theoretical: 16.91526 KDa
Sequence	String: MAFVACGPLR AGEGGARLGA RKAACSMQLA PPGIPPGEDA RNNQSLRQYV ARPVETYQKR SFATPLPLTW TGETETVGAF DVVVPPQEK DLPVSGEATS AFVKYSDMVR AERKAALQAL LSASAAGEGR PTCGAEGRKF VSNANPVLVN GVKCVEYWRK UniProtKB: Uncharacterized protein

+
Macromolecule #27: LRC5

Macromolecule	Name: LRC5 / type: protein_or_peptide / ID: 27 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)	Organism: Porphyridium purpureum (eukaryote)
Molecular weight	Theoretical: 30.272959 KDa
Sequence	String: MAFVSGAGVA VPAGAKASAP LCALRMSGYG DYSYSTDRTK GHVNQYYVDK ARSRSDWGNR NVLPASEGDA VLGRTAKGAV AVPEFGIPQ LDDPVLGFGP DSMVDPRIAE ADGAVWRWDA GFVDESMTLA SCADISDEAV ADEAFAKFRG SVLAERGAMI T KAESATAS ...String: MAFVSGAGVA VPAGAKASAP LCALRMSGYG DYSYSTDRTK GHVNQYYVDK ARSRSDWGNR NVLPASEGDA VLGRTAKGAV AVPEFGIPQ LDDPVLGFGP DSMVDPRIAE ADGAVWRWDA GFVDESMTLA SCADISDEAV ADEAFAKFRG SVLAERGAMI T KAESATAS VITSLRDGLY SGEAQLLTAS GQRLANVAGQ EKIATISGYT WDGQPQTEIP GKPFVKSIGA MDYMDGVEGG DV VAAKVGA FWKPKAPKEV PYKRPMGANT PELPYNTVPR LVQAAGLAVQ E UniProtKB: Uncharacterized protein

+
Macromolecule #28: FAS1 domain-containing protein

Macromolecule	Name: FAS1 domain-containing protein / type: protein_or_peptide / ID: 28 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)	Organism: Porphyridium purpureum (eukaryote)
Molecular weight	Theoretical: 26.620727 KDa
Sequence	String: MAGFVASTGA VLRKQQVDAA PVCERASSRA RAVAPLSMAR TAYPYTGSGY GSAGVPYGQD TYGYKATTAK SITETAAQAG VFNTFVKLL NESGVEKLVE QAGPYTVFAP TDDAFAALLE PHSFNKLATL LRPENNDALR KVLMHHVIPG AFTSASLMDR A VTVKSLAG ...String: MAGFVASTGA VLRKQQVDAA PVCERASSRA RAVAPLSMAR TAYPYTGSGY GSAGVPYGQD TYGYKATTAK SITETAAQAG VFNTFVKLL NESGVEKLVE QAGPYTVFAP TDDAFAALLE PHSFNKLATL LRPENNDALR KVLMHHVIPG AFTSASLMDR A VTVKSLAG EPISIMGLNK LVTAGTAKVV RADVPCANGC IIHAVSSVII PPNYVPVPQP TKPVFPRSVI AEIAKLPTPR QA LGLDPAP SKIVKY UniProtKB: FAS1 domain-containing protein

+
Macromolecule #29: PHYCOERYTHROBILIN

Macromolecule	Name: PHYCOERYTHROBILIN / type: ligand / ID: 29 / Number of copies: 1298 / Formula: PEB
Molecular weight	Theoretical: 588.694 Da
Chemical component information	ChemComp-PEB: PHYCOERYTHROBILIN / Phycoerythrobilin

+
Macromolecule #30: PHYCOUROBILIN

Macromolecule	Name: PHYCOUROBILIN / type: ligand / ID: 30 / Number of copies: 40 / Formula: PUB
Molecular weight	Theoretical: 590.71 Da
Chemical component information	ChemComp-CYB: PHYCOUROBILIN / Phycourobilin

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Macromolecule #31: PHYCOCYANOBILIN

Macromolecule	Name: PHYCOCYANOBILIN / type: ligand / ID: 31 / Number of copies: 120 / Formula: CYC
Molecular weight	Theoretical: 588.694 Da
Chemical component information	ChemComp-CYC: PHYCOCYANOBILIN / Phycocyanobilin

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Macromolecule #32: phenylmethanesulfonic acid

Macromolecule	Name: phenylmethanesulfonic acid / type: ligand / ID: 32 / Number of copies: 4 / Formula: PMS
Molecular weight	Theoretical: 172.202 Da
Chemical component information	ChemComp-IOX: phenylmethanesulfonic acid

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Experimental details

-
Structure determination

Method	cryo EM
Processing	single particle reconstruction
Aggregation state	particle

-
Sample preparation

Buffer

pH: 7
Component:

Concentration	Formula	Name
0.75 mol/L	Na₂HPO₄	disodium hydrogen phosphate
0.75 mol/L	KH₂PO₄	monopotassium phosphate

Grid

Model: PELCO Ultrathin Carbon with Lacey Carbon / Material: COPPER / Mesh: 200 / Support film - Material: CARBON / Support film - topology: LACEY / Support film - Film thickness: 2 / Pretreatment - Type: GLOW DISCHARGE / Pretreatment - Time: 30 sec. / Pretreatment - Atmosphere: AIR / Pretreatment - Pressure: 0.1 kPa

Vitrification

Cryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 18 K / Instrument: FEI VITROBOT MARK IV

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Electron microscopy

Microscope	FEI TITAN KRIOS
Electron beam	Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron optics	C2 aperture diameter: 100.0 µm / Calibrated defocus max: 2.3000000000000003 µm / Calibrated defocus min: 1.3 µm / Calibrated magnification: 130000 / Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy / Cs: 2.7 mm / Nominal defocus max: 2.3000000000000003 µm / Nominal defocus min: 1.3 µm / Nominal magnification: 130000
Specialist optics	Energy filter - Name: GIF Quantum ER
Sample stage	Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN
Temperature	Min: 70.0 K / Max: 70.0 K
Image recording	Film or detector model: GATAN K2 SUMMIT (4k x 4k) / Detector mode: SUPER-RESOLUTION / Digitization - Dimensions - Width: 4096 pixel / Digitization - Dimensions - Height: 4096 pixel / Digitization - Frames/image: 1-32 / Number grids imaged: 2 / Number real images: 6438 / Average exposure time: 8.2 sec. / Average electron dose: 50.0 e/Å²
Experimental equipment	Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Particle selection	Number selected: 315046
Startup model	Type of model: PDB ENTRY PDB model - PDB ID: 6kgx
Initial angle assignment	Type: MAXIMUM LIKELIHOOD / Software - Name: RELION (ver. 3.1beta)
Final 3D classification	Number classes: 5 / Avg.num./class: 87399 / Software - Name: RELION (ver. 3.1beta)
Final angle assignment	Type: MAXIMUM LIKELIHOOD / Software - Name: RELION (ver. 3.1beta)
Final reconstruction	Number classes used: 2 / Applied symmetry - Point group: C₂ (2 fold cyclic) / Algorithm: FOURIER SPACE / Resolution.type: BY AUTHOR / Resolution: 3.0 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: RELION (ver. 3.1beta) / Number images used: 87399

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Atomic model buiding 1

Initial model	PDB ID: 6kgx Chain - Source name: PDB / Chain - Initial model type: experimental model
Refinement	Space: REAL / Protocol: RIGID BODY FIT / Overall B value: 62.05 / Target criteria: 0,99
Output model	PDB-7ezx: Structure of the phycobilisome from the red alga Porphyridium purpureum in Middle Light

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-Sample components

+Entire : phycobilisome

+Supramolecule #1: phycobilisome

+Macromolecule #1: Phycobilisome 31.8 kDa linker polypeptide, phycoerythrin-associat...

+Macromolecule #2: R-phycoerythrin gamma chain, chloroplastic

+Macromolecule #3: Phycobilisome rod-core linker polypeptide

+Macromolecule #4: C-phycocyanin alpha subunit

+Macromolecule #5: C-phycocyanin beta subunit

+Macromolecule #6: Phycoerythrin alpha subunit

+Macromolecule #7: B-phycoerythrin beta chain

+Macromolecule #8: Phycobilisome 31.8 kDa linker polypeptide, phycoerythrin-associat...

+Macromolecule #9: Phycobilisome 31.8 kDa linker polypeptide, phycoerythrin-associat...

+Macromolecule #10: Phycobilisome 27.9 kDa linker polypeptide, phycoerythrin-associat...

+Macromolecule #11: CaRSPs2

+Macromolecule #12: CaRSPs1

+Macromolecule #13: FAS1 domain-containing protein

+Macromolecule #14: R-phycoerythrin gamma chain, chloroplastic

+Macromolecule #15: Phycobilisome 32.1 kDa linker polypeptide, phycocyanin-associated, rod

+Macromolecule #16: R-phycoerythrin gamma chain, chloroplastic

+Macromolecule #17: R-phycoerythrin gamma chain, chloroplastic

+Macromolecule #18: Phycobilisome 27.9 kDa linker polypeptide, phycoerythrin-associat...

+Macromolecule #19: Phycobilisome 31.8 kDa linker polypeptide, phycoerythrin-associat...

+Macromolecule #20: Allophycocyanin alpha subunit

+Macromolecule #21: Allophycocyanin beta subunit

+Macromolecule #22: Allophycocyanin gamma subunit

+Macromolecule #23: Allophycocyanin beta 18 subunit

+Macromolecule #24: Phycobilisome 7.8 kDa linker polypeptide, allophycocyanin-associa...

+Macromolecule #25: Phycobilisome linker polypeptide

+Macromolecule #26: Lrc4

+Macromolecule #27: LRC5

+Macromolecule #28: FAS1 domain-containing protein

+Macromolecule #29: PHYCOERYTHROBILIN

+Macromolecule #30: PHYCOUROBILIN

+Macromolecule #31: PHYCOCYANOBILIN

+Macromolecule #32: phenylmethanesulfonic acid

-Experimental details

-Structure determination

-Sample preparation

-Electron microscopy

-Image processing

-Atomic model buiding 1

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