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Open data
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Basic information
Entry | Database: EMDB / ID: EMD-30589 | |||||||||||||||||||||||||||
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Title | Cryo-EM Structure of human CALHM5 in the presence of Ca2+ | |||||||||||||||||||||||||||
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Function / homology | Calcium homeostasis modulator family / Calcium homeostasis modulator / monoatomic cation channel activity / plasma membrane / Calcium homeostasis modulator protein 5![]() | |||||||||||||||||||||||||||
Biological species | ![]() | |||||||||||||||||||||||||||
Method | single particle reconstruction / cryo EM / Resolution: 2.94 Å | |||||||||||||||||||||||||||
![]() | Liu J / Guan FH / Wu J / Wan FT / Lei M / Ye S | |||||||||||||||||||||||||||
Funding support | ![]()
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![]() | ![]() Title: Cryo-EM structures of human calcium homeostasis modulator 5. Authors: Jie Liu / Futang Wan / Qiuheng Jin / Xiaoxiao Li / Eijaz Ahmed Bhat / Jiangtao Guo / Ming Lei / Fenghui Guan / Jian Wu / Sheng Ye / ![]() | |||||||||||||||||||||||||||
History |
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Structure visualization
Movie |
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Structure viewer | EM map: ![]() ![]() ![]() |
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 135.6 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 11.6 KB 11.6 KB | Display Display | ![]() |
Images | ![]() | 82.4 KB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Validation report
Summary document | ![]() | 485.8 KB | Display | ![]() |
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Full document | ![]() | 485.4 KB | Display | |
Data in XML | ![]() | 6.6 KB | Display | |
Data in CIF | ![]() | 7.5 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 7d65MC ![]() 7d60C ![]() 7d61C M: atomic model generated by this map C: citing same article ( |
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Similar structure data |
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Links
EMDB pages | ![]() ![]() |
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Map
File | ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Voxel size | X=Y=Z: 1.1 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
CCP4 map header:
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-Supplemental data
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Sample components
-Entire : Calcium homeostasis modulator protein 5
Entire | Name: Calcium homeostasis modulator protein 5 |
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Components |
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-Supramolecule #1: Calcium homeostasis modulator protein 5
Supramolecule | Name: Calcium homeostasis modulator protein 5 / type: organelle_or_cellular_component / ID: 1 / Parent: 0 / Macromolecule list: #1 |
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Source (natural) | Organism: ![]() |
Recombinant expression | Organism: ![]() |
-Macromolecule #1: Calcium homeostasis modulator protein 5
Macromolecule | Name: Calcium homeostasis modulator protein 5 / type: protein_or_peptide / ID: 1 / Number of copies: 11 / Enantiomer: LEVO |
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Source (natural) | Organism: ![]() |
Molecular weight | Theoretical: 32.921305 KDa |
Recombinant expression | Organism: ![]() |
Sequence | String: MDAFQGILKF FLNQKTVIGY SFMALLTVGS ERLFSVVAFK CPCSTENMTY GLVFLFAPAW VLLILGFFLN NRSWRLFTGC CVNPRKIFP RGHSCRFFYV LGQITLSSLV APVMWLSVAL LNGTFYECAM SGTRSSGLLE LICKGKPKEC WEELHKVSCG K TSMLPTVN ...String: MDAFQGILKF FLNQKTVIGY SFMALLTVGS ERLFSVVAFK CPCSTENMTY GLVFLFAPAW VLLILGFFLN NRSWRLFTGC CVNPRKIFP RGHSCRFFYV LGQITLSSLV APVMWLSVAL LNGTFYECAM SGTRSSGLLE LICKGKPKEC WEELHKVSCG K TSMLPTVN EELKLSLQAQ SQILGWCLIC SASFFSLLTT CYARCRSKVS YLQLSFWKTY AQKEKEQLEN TFLDYANKLS ER NLKCFFE NKRPDPFPMP TFAAWEAASE LHSFHQSQQH YSTLHRVVDN G |
-Macromolecule #2: 1,2-DIOCTANOYL-SN-GLYCERO-3-PHOSPHATE
Macromolecule | Name: 1,2-DIOCTANOYL-SN-GLYCERO-3-PHOSPHATE / type: ligand / ID: 2 / Number of copies: 55 / Formula: PA8 |
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Molecular weight | Theoretical: 423.458 Da |
Chemical component information | ![]() ChemComp-PA8: |
-Experimental details
-Structure determination
Method | cryo EM |
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![]() | single particle reconstruction |
Aggregation state | particle |
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Sample preparation
Concentration | 3 mg/mL |
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Buffer | pH: 7.5 |
Vitrification | Cryogen name: ETHANE |
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Electron microscopy
Microscope | FEI TITAN KRIOS |
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Image recording | Film or detector model: GATAN K3 (6k x 4k) / Average electron dose: 50.0 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |
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Image processing
Final reconstruction | Resolution.type: BY AUTHOR / Resolution: 2.94 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 392239 |
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Initial angle assignment | Type: COMMON LINE |
Final angle assignment | Type: COMMON LINE |