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Open data
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Basic information
Entry | ![]() | |||||||||||||||||||||
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Title | Golden shiner reovirus inner capsid particle | |||||||||||||||||||||
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![]() | dsRNA virus / aquareovirus / RNA-dependent RNA-polymerase / VIRUS | |||||||||||||||||||||
Biological species | ![]() | |||||||||||||||||||||
Method | single particle reconstruction / cryo EM / Resolution: 4.2 Å | |||||||||||||||||||||
![]() | Zhou ZH / Stevens AW | |||||||||||||||||||||
Funding support | ![]()
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![]() | ![]() Title: Asymmetric reconstruction of the aquareovirus core at near-atomic resolution and mechanism of transcription initiation. Authors: Alexander Stevens / Yanxiang Cui / Sakar Shivakoti / Z Hong Zhou / ![]() | |||||||||||||||||||||
History |
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Structure visualization
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 1.8 GB | ![]() | |
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Header (meta data) | ![]() ![]() | 16.3 KB 16.3 KB | Display Display | ![]() |
FSC (resolution estimation) | ![]() | 28.2 KB | Display | ![]() |
Images | ![]() | 88.2 KB | ||
Filedesc metadata | ![]() | 4.3 KB | ||
Others | ![]() ![]() | 1.6 GB 1.6 GB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Validation report
Summary document | ![]() | 1.3 MB | Display | ![]() |
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Full document | ![]() | 1.3 MB | Display | |
Data in XML | ![]() | 36.1 KB | Display | |
Data in CIF | ![]() | 49.1 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
EMDB pages | ![]() ![]() |
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Map
File | ![]() | ||||||||||||||||||||
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Voxel size | X=Y=Z: 1.36 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Half map: #2
File | emd_29337_half_map_1.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Half map: #1
File | emd_29337_half_map_2.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
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Sample components
-Entire : Golden shiner reovirus
Entire | Name: ![]() |
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Components |
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-Supramolecule #1: Golden shiner reovirus
Supramolecule | Name: Golden shiner reovirus / type: virus / ID: 1 / Parent: 0 / Macromolecule list: #1-#10 / Details: Purified from infected cells / NCBI-ID: 185783 / Sci species name: Golden shiner reovirus / Sci species strain: AQRVC / Virus type: VIRION / Virus isolate: STRAIN / Virus enveloped: No / Virus empty: No |
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Host (natural) | Organism: ![]() |
Molecular weight | Theoretical: 47 MDa |
Virus shell | Shell ID: 1 / Name: capsid / Diameter: 750.0 Å / T number (triangulation number): 13 |
-Experimental details
-Structure determination
Method | cryo EM |
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![]() | single particle reconstruction |
Aggregation state | particle |
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Sample preparation
Buffer | pH: 7.4 / Details: Phosphate buffered saline |
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Grid | Model: Quantifoil R2/1 / Support film - Material: CARBON / Support film - topology: HOLEY / Pretreatment - Type: GLOW DISCHARGE |
Vitrification | Cryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 277 K / Instrument: FEI VITROBOT MARK IV |
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Electron microscopy
Microscope | TFS KRIOS |
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Image recording | Film or detector model: GATAN K2 SUMMIT (4k x 4k) / Detector mode: SUPER-RESOLUTION / Average electron dose: 45.0 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | C2 aperture diameter: 50.0 µm / Calibrated defocus max: 2.5 µm / Calibrated defocus min: 1.5 µm / Calibrated magnification: 81000 / Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Cs: 2.7 mm / Nominal defocus max: 2.5 µm / Nominal defocus min: 1.5 µm |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |