[English] 日本語
![](img/lk-miru.gif)
- EMDB-26344: YcaO-mediated ATP-dependent peptidase activity in ribosomal pepti... -
+
Open data
-
Basic information
Entry | ![]() | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
Title | YcaO-mediated ATP-dependent peptidase activity in ribosomal peptide biosynthesis | |||||||||
![]() | ||||||||||
![]() |
| |||||||||
![]() | Ripps / Ycao domain / musD / Cyanobactin / HYDROLASE | |||||||||
Function / homology | Bacteriocin biosynthesis, cyclodehydratase domain / Thiazole/oxazole-forming peptide maturase, SagD family component / ubiquitin-like modifier activating enzyme activity / YcaO-like domain / YcaO cyclodehydratase, ATP-ad Mg2+-binding / YcaO domain profile. / Ubiquitin-activating enzyme / MusD![]() | |||||||||
Biological species | ![]() | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 3.1 Å | |||||||||
![]() | Zheng Y / Nair SK | |||||||||
Funding support | ![]()
| |||||||||
![]() | ![]() Title: YcaO-mediated ATP-dependent peptidase activity in ribosomal peptide biosynthesis. Authors: Yiwu Zheng / Satish K Nair / ![]() Abstract: YcaO enzymes catalyze ATP-dependent post-translation modifications on peptides, including the installation of (ox/thi)azoline, thioamide and/or amidine moieties. Here we demonstrate that, in the ...YcaO enzymes catalyze ATP-dependent post-translation modifications on peptides, including the installation of (ox/thi)azoline, thioamide and/or amidine moieties. Here we demonstrate that, in the biosynthesis of the bis-methyloxazolic alkaloid muscoride A, the YcaO enzyme MusD carries out both ATP-dependent cyclodehydration and peptide bond cleavage, which is a mechanism unprecedented for such a reaction. YcaO-catalyzed modifications are proposed to occur through a backbone O-phosphorylated intermediate, but this mechanism remains speculative. We report, to our knowedge, the first characterization of an acyl-phosphate species consistent with the proposed mechanism for backbone amide activation. The 3.1-Å-resolution cryogenic electron microscopy structure of MusD along with biochemical analysis allow identification of residues that enable peptide cleavage reaction. Bioinformatics analysis identifies other cyanobactin pathways that may deploy bifunctional YcaO enzymes. Our structural, mutational and mechanistic studies expand the scope of modifications catalyzed by YcaO proteins to include peptide hydrolysis and provide evidence for a unifying mechanism for the catalytically diverse outcomes. | |||||||||
History |
|
-
Structure visualization
Supplemental images |
---|
-
Downloads & links
-EMDB archive
Map data | ![]() | 62.5 MB | ![]() | |
---|---|---|---|---|
Header (meta data) | ![]() ![]() | 14.4 KB 14.4 KB | Display Display | ![]() |
FSC (resolution estimation) | ![]() | 10.5 KB | Display | ![]() |
Images | ![]() | 104.4 KB | ||
Masks | ![]() | 125 MB | ![]() | |
Filedesc metadata | ![]() | 5.7 KB | ||
Others | ![]() ![]() | 115.7 MB 115.7 MB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Validation report
Summary document | ![]() | 819.7 KB | Display | ![]() |
---|---|---|---|---|
Full document | ![]() | 819.2 KB | Display | |
Data in XML | ![]() | 18.8 KB | Display | |
Data in CIF | ![]() | 24 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 7u58MC M: atomic model generated by this map C: citing same article ( |
---|---|
Similar structure data | Similarity search - Function & homology ![]() |
-
Links
EMDB pages | ![]() ![]() |
---|---|
Related items in Molecule of the Month |
-
Map
File | ![]() | ||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Voxel size | X=Y=Z: 1.054 Å | ||||||||||||||||||||
Density |
| ||||||||||||||||||||
Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
|
-Supplemental data
-Mask #1
File | ![]() | ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Projections & Slices |
| ||||||||||||
Density Histograms |
-Half map: #1
File | emd_26344_half_map_1.map | ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Projections & Slices |
| ||||||||||||
Density Histograms |
-Half map: #2
File | emd_26344_half_map_2.map | ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Projections & Slices |
| ||||||||||||
Density Histograms |
-
Sample components
-Entire : MusD
Entire | Name: MusD |
---|---|
Components |
|
-Supramolecule #1: MusD
Supramolecule | Name: MusD / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1 |
---|---|
Source (natural) | Organism: ![]() |
Molecular weight | Theoretical: 88.76 kDa/nm |
-Macromolecule #1: MusD
Macromolecule | Name: MusD / type: protein_or_peptide / ID: 1 / Number of copies: 2 / Enantiomer: LEVO |
---|---|
Source (natural) | Organism: ![]() |
Molecular weight | Theoretical: 88.844805 KDa |
Recombinant expression | Organism: ![]() ![]() |
Sequence | String: SNAMKILQIK PHFRAEIIEP KHVYLLSESS THALTGELYC QLIPLLNGNY TVDEIINKLQ VDPSHIDYAL ERLQARGYIT EAIPQLTPE AVAFWGLLKV EPQVAYQCLQ QTQVYVSSVV NLPTQPLITA LEEVGIKAIN WDGELQEFPP HSLLVVLTDD Y LQPQLNKI ...String: SNAMKILQIK PHFRAEIIEP KHVYLLSESS THALTGELYC QLIPLLNGNY TVDEIINKLQ VDPSHIDYAL ERLQARGYIT EAIPQLTPE AVAFWGLLKV EPQVAYQCLQ QTQVYVSSVV NLPTQPLITA LEEVGIKAIN WDGELQEFPP HSLLVVLTDD Y LQPQLNKI NQIALKANQP WLLIKPVGTI LWLGPIFQPQ ITGCWECLAQ RLRVNREVEA SVLRQKNSSL QLSPSQELNS SV LQSNGNG VKSEVIECLP PPAAVIPSTL QTALHLATTE IAKWIVKQGV EDTTPFPTLE GKVITFDQRN LDLQTHILSL RPQ CPSCGN PNLLTERAFQ PLVLSSRKKQ FTSDGGHRAF SPDQTVNRYQ HLISPITGVV TSLVRASDPN DSLNHTYNAV HSFV IASNI GRMRRYLKHK SSGKGKTDSQ SKASGFCEAI ERYSGVYQGD EPRISATLAE LGEKAIHPAR CSLFSSEQYE YREEF NRRG GVFDWIPQPF DETKVIEWTP VWSLTEQTHK YIPTAYCYYG YPLPEDHEFC RANSNGDATG NTLEEAIIQG FFEIVE RDS VAIWWYNRLK RPAVDLASFN EPYLLEVQDL YRSNNRDLWV IDITADLDIP TFVAVSYLKD NKHQTILLGF GTHFDPK IA ILRAVTEVNQ IAFTCDGVEV TKEFVEMREW FKKATIENQP YLVPDSTVPA KVYQDYQQRW SDDIYEDVMT CVEISKNA G LETLVLDKTR PDIGLNVAKV IVPEMPHYWL RMGAKRIYDV PVKMGWLSTP LTEEQMNPIS VPI UniProtKB: MusD |
-Macromolecule #2: ZINC ION
Macromolecule | Name: ZINC ION / type: ligand / ID: 2 / Number of copies: 2 / Formula: ZN |
---|---|
Molecular weight | Theoretical: 65.409 Da |
-Macromolecule #3: MAGNESIUM ION
Macromolecule | Name: MAGNESIUM ION / type: ligand / ID: 3 / Number of copies: 4 / Formula: MG |
---|---|
Molecular weight | Theoretical: 24.305 Da |
-Experimental details
-Structure determination
Method | cryo EM |
---|---|
![]() | single particle reconstruction |
Aggregation state | 2D array |
-
Sample preparation
Concentration | 6 mg/mL |
---|---|
Buffer | pH: 7.5 / Details: HEPES pH 7.5. 25 mM KCl. |
Grid | Material: COPPER |
Vitrification | Cryogen name: ETHANE-PROPANE |
-
Electron microscopy
Microscope | FEI TITAN |
---|---|
Image recording | Film or detector model: GATAN K3 (6k x 4k) / Average electron dose: 1.33 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Illumination mode: OTHER / Imaging mode: DIFFRACTION / Nominal defocus max: 2.0 µm / Nominal defocus min: 0.6 µm |