- EMDB-24701: Metazoan pre-targeting GET complex (cBUGG-out) -
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Basic information
Entry
Database: EMDB / ID: EMD-24701
Title
Metazoan pre-targeting GET complex (cBUGG-out)
Map data
Sharpened cBUGG-out (C2)
Sample
Complex: metazoan pre-targeting GET complex cBUGG-out
Protein or peptide: ATPase GET3
Protein or peptide: Golgi to ER traffic protein 4 homolog
Protein or peptide: Large proline-rich protein BAG6
Protein or peptide: Ubiquitin-like protein 4A
Ligand: ADENOSINE-5'-TRIPHOSPHATE
Ligand: MAGNESIUM ION
Ligand: ZINC ION
Keywords
ATPase / complex / membrane protein chaperone / CHAPERONE
Function / homology
Function and homology information
BAT3 complex / immune response-activating cell surface receptor signaling pathway / maintenance of unfolded protein / GET complex / ubiquitin-like protein transferase activity / tail-anchored membrane protein insertion into ER membrane / positive regulation of ERAD pathway / cytoplasmic sequestering of protein / Hydrolases; Acting on acid anhydrides / synaptonemal complex assembly ...BAT3 complex / immune response-activating cell surface receptor signaling pathway / maintenance of unfolded protein / GET complex / ubiquitin-like protein transferase activity / tail-anchored membrane protein insertion into ER membrane / positive regulation of ERAD pathway / cytoplasmic sequestering of protein / Hydrolases; Acting on acid anhydrides / synaptonemal complex assembly / protein insertion into ER membrane / post-translational protein targeting to endoplasmic reticulum membrane / internal peptidyl-lysine acetylation / misfolded protein binding / natural killer cell activation / endoplasmic reticulum stress-induced pre-emptive quality control / Insertion of tail-anchored proteins into the endoplasmic reticulum membrane / proteasome binding / ubiquitin-specific protease binding / regulation of embryonic development / polyubiquitin modification-dependent protein binding / intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress / intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator / proteasomal protein catabolic process / ERAD pathway / negative regulation of proteasomal ubiquitin-dependent protein catabolic process / synapse assembly / Hsp70 protein binding / kidney development / molecular function activator activity / negative regulation of proteolysis / lung development / protein modification process / regulation of protein stability / brain development / ribosome binding / chromatin organization / chromosome / protein-folding chaperone binding / ubiquitin-dependent protein catabolic process / spermatogenesis / proteasome-mediated ubiquitin-dependent protein catabolic process / molecular adaptor activity / cell differentiation / protein stabilization / intracellular membrane-bounded organelle / signaling receptor binding / ubiquitin protein ligase binding / nucleolus / negative regulation of apoptotic process / apoptotic process / ATP hydrolysis activity / extracellular exosome / nucleoplasm / ATP binding / identical protein binding / membrane / nucleus / metal ion binding / cytosol / cytoplasm Similarity search - Function
Ubl4, C-terminal TUGS domain / UBL4A-like, ubiquitin-like domain / Tethering Ubl4a to BAGS domain / Golgi to ER traffic protein 4 / Large proline-rich protein BAG6 / : / Golgi to ER traffic protein 4 / BCL2-associated athanogene 6 / Bag6, BAG-similar domain / Arsenical pump ATPase, ArsA/GET3, eukaryotic ...Ubl4, C-terminal TUGS domain / UBL4A-like, ubiquitin-like domain / Tethering Ubl4a to BAGS domain / Golgi to ER traffic protein 4 / Large proline-rich protein BAG6 / : / Golgi to ER traffic protein 4 / BCL2-associated athanogene 6 / Bag6, BAG-similar domain / Arsenical pump ATPase, ArsA/GET3, eukaryotic / Arsenical pump ATPase, ArsA/GET3 / Anion-transporting ATPase-like domain / Anion-transporting ATPase / Ubiquitin domain signature. / Ubiquitin conserved site / Ubiquitin domain / Ubiquitin family / Ubiquitin homologues / Tetratricopeptide-like helical domain superfamily / Ubiquitin domain profile. / Ubiquitin-like domain / Ubiquitin-like domain superfamily / P-loop containing nucleoside triphosphate hydrolase Similarity search - Domain/homology
Ubiquitin-like protein 4A / Large proline-rich protein BAG6 / ATPase GET3 / Golgi to ER traffic protein 4 homolog Similarity search - Component
Biological species
Danio rerio (zebrafish) / Homo sapiens (human)
Method
single particle reconstruction / cryo EM / Resolution: 3.4 Å
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)
DP2GM137415
American Heart Association
287375208
Citation
Journal: Nat Struct Mol Biol / Year: 2021 Title: Structural insights into metazoan pretargeting GET complexes. Authors: Alexander F A Keszei / Matthew C J Yip / Ta-Chien Hsieh / Sichen Shao / Abstract: Close coordination between chaperones is essential for protein biosynthesis, including the delivery of tail-anchored (TA) proteins containing a single C-terminal transmembrane domain to the ...Close coordination between chaperones is essential for protein biosynthesis, including the delivery of tail-anchored (TA) proteins containing a single C-terminal transmembrane domain to the endoplasmic reticulum (ER) by the conserved GET pathway. For successful targeting, nascent TA proteins must be promptly chaperoned and loaded onto the cytosolic ATPase Get3 through a transfer reaction involving the chaperone SGTA and bridging factors Get4, Ubl4a and Bag6. Here, we report cryo-electron microscopy structures of metazoan pretargeting GET complexes at 3.3-3.6 Å. The structures reveal that Get3 helix 8 and the Get4 C terminus form a composite lid over the Get3 substrate-binding chamber that is opened by SGTA. Another interaction with Get4 prevents formation of Get3 helix 4, which links the substrate chamber and ATPase domain. Both interactions facilitate TA protein transfer from SGTA to Get3. Our findings show how the pretargeting complex primes Get3 for coordinated client loading and ER targeting.
History
Deposition
Aug 16, 2021
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Header (metadata) release
Dec 15, 2021
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Map release
Dec 15, 2021
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Update
Jun 5, 2024
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Current status
Jun 5, 2024
Processing site: RCSB / Status: Released
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Structure visualization
Movie
Surface view with section colored by density value
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