EMDB-19040: Structure of Integrator-PP2A-SOSS-CTD post-termination complex

Basic information

Entry

Database: EMDB / ID: EMD-19040

• Title: Structure of Integrator-PP2A-SOSS-CTD post-termination complex
• Map data: Overall map filtered by local resolution

Sample

• Complex: Post-termination complex
  • Complex: Integrator-PP2A complex and NABP2 and INIP
    • Protein or peptide: x 2 types
  • Complex: RNA Polymerase II C-terminal domain peptides
    • Protein or peptide: x 18 types
  • Complex: DSS1
    • Protein or peptide: x 1 types
• Ligand: x 2 types

• Keywords: Integrator complex / Post-termination complex / RNA Polymerase II / Pol II / RNAPII / TRANSCRIPTION

Function / homology

Function:

SOSS complex / U2 snRNA 3'-end processing / regulation of fertilization / meiotic spindle elongation / Integration of energy metabolism / PP2A-mediated dephosphorylation of key metabolic factors / regulation of microtubule binding / snRNA 3'-end processing / MASTL Facilitates Mitotic Progression / regulation of meiotic cell cycle process involved in oocyte maturation / mitotic sister chromatid separation / protein serine/threonine phosphatase complex / protein phosphatase type 2A complex / meiotic sister chromatid cohesion, centromeric / establishment of protein localization to telomere / snRNA processing / peptidyl-serine dephosphorylation / peptidyl-threonine dephosphorylation / flagellated sperm motility / FAR/SIN/STRIPAK complex / protein localization to nuclear envelope / Regulation of glycolysis by fructose 2,6-bisphosphate metabolism / positive regulation of microtubule binding / Inhibition of replication initiation of damaged DNA by RB1/E2F1 / female meiotic nuclear division / protein phosphatase regulator activity / protein antigen binding / GABA receptor binding / integrator complex / regulation of transcription elongation by RNA polymerase II / APC truncation mutants have impaired AXIN binding / AXIN missense mutants destabilize the destruction complex / Truncations of AMER1 destabilize the destruction complex / Formation of RNA Pol II elongation complex / Formation of the Early Elongation Complex / Transcriptional regulation by small RNAs / RNA Polymerase II Pre-transcription Events / TP53 Regulates Transcription of DNA Repair Genes / FGFR2 alternative splicing / RNA polymerase II transcribes snRNA genes / mRNA Capping / mRNA Splicing - Minor Pathway / Processing of Capped Intron-Containing Pre-mRNA / RNA Polymerase II Promoter Escape / RNA Polymerase II Transcription Pre-Initiation And Promoter Opening / RNA Polymerase II Transcription Initiation / RNA Polymerase II Transcription Elongation / RNA Polymerase II Transcription Initiation And Promoter Clearance / RNA Pol II CTD phosphorylation and interaction with CE / Estrogen-dependent gene expression / Formation of TC-NER Pre-Incision Complex / Dual incision in TC-NER / Gap-filling DNA repair synthesis and ligation in TC-NER / mRNA Splicing - Major Pathway / Initiation of Nuclear Envelope (NE) Reformation / positive regulation of extrinsic apoptotic signaling pathway in absence of ligand / ERKs are inactivated / G-rich strand telomeric DNA binding / Beta-catenin phosphorylation cascade / Signaling by GSK3beta mutants / CTNNB1 S33 mutants aren't phosphorylated / CTNNB1 S37 mutants aren't phosphorylated / CTNNB1 S45 mutants aren't phosphorylated / CTNNB1 T41 mutants aren't phosphorylated / mitotic G2/M transition checkpoint / Hydrolases; Acting on ester bonds; Endoribonucleases producing 3'-phosphomonoesters / regulation of growth / Disassembly of the destruction complex and recruitment of AXIN to the membrane / inner cell mass cell proliferation / negative regulation of epithelial to mesenchymal transition / centrosome localization / negative regulation of glycolytic process through fructose-6-phosphate / positive regulation of NLRP3 inflammasome complex assembly / response to ionizing radiation / Platelet sensitization by LDL / CTLA4 inhibitory signaling / protein serine/threonine phosphatase activity / myosin phosphatase activity / protein-serine/threonine phosphatase / regulation of cell differentiation / T cell homeostasis / ERK/MAPK targets / mesoderm development / regulation of G1/S transition of mitotic cell cycle / positive regulation of telomere capping / phosphoprotein phosphatase activity / RNA polymerase II transcribes snRNA genes / DARPP-32 events / chromosome, centromeric region / negative regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction / lateral plasma membrane / negative regulation of hippo signaling / RNA polymerase II, core complex / Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) / Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal / DNA polymerase binding / Cyclin A/B1/B2 associated events during G2/M transition / core promoter sequence-specific DNA binding / Mitotic Prometaphase / EML4 and NUDC in mitotic spindle formation

Domain/homology:

SOSS complex subunit C / SOSS complex subunit C / Integrator complex subunit 3, N-terminal / Integrator complex subunit 3 / Integrator complex subunit 3 N-terminal / : / Single-stranded DNA binding protein Ssb-like, OB fold / Domain of unknown function DUF3677 / Integrator complex subunit 2, metazoa / Integrator complex subunit 2 / Integrator complex subunit 7 / Integrator complex subunit 1 / : / : / : / Integrator complex subunit 1, RP2B-binding domain / Integrator complex subunit 2 / Integrator complex subunit 1, R3 domain / Integrator complex subunit 1, R4 domain / Integrator complex subunit 1, INTS2-binding domain / Integrator complex subunit 7, C-terminal domain / : / INTS6/SAGE1/DDX26B/CT45, C-terminal / INTS6/SAGE1/DDX26B/CT45 C-terminus / Integrator complex subunit 10 / Integrator complex subunit 15 / Integrator complex subunit 5, C-terminal / Integrator complex subunit 5, N-terminal / Integrator complex subunit 8 / Integrator complex subunit 14 / Integrator complex subunit 5 / Integrator complex subunit 14, beta-barrel domain / Integrator complex subunit 14, C-terminal / Integrator complex subunit 5 N-terminus / Integrator complex subunit 5 C-terminus / Integrator complex subunit 15 / Integrator complex subunit 14, beta-barrel domain / Integrator complex subunit 14, alpha-helical domain / Integrator subunit 10 / Cell cycle regulator Mat89Bb / Cell cycle and development regulator Mat89Bb / : / Sister chromatid cohesion protein PDS5 protein / Integrator complex subunit 9 / : / Integrator IntS9, C-terminal domain / Integrator complex subunit 11, MBL-fold / : / Integrator IntS11, C-terminal domain / : / : / Metallo-beta-lactamase superfamily domain / : / Beta-Casp domain / Beta-Casp domain / Beta-Casp domain / Zn-dependent metallo-hydrolase, RNA specificity domain / Zn-dependent metallo-hydrolase RNA specificity domain / HEAT repeat / HEAT repeat / PPP2R1A-like HEAT repeat / Serine/threonine specific protein phosphatases signature. / Protein phosphatase 2A homologues, catalytic domain. / Serine/threonine-specific protein phosphatase/bis(5-nucleosyl)-tetraphosphatase / HEAT repeat profile. / HEAT, type 2 / von Willebrand factor type A domain / HEAT repeats / Calcineurin-like phosphoesterase domain, ApaH type / Calcineurin-like phosphoesterase / Metallo-dependent phosphatase-like / RNA polymerase II, heptapeptide repeat, eukaryotic / RNA polymerase Rpb1, domain 6 / RNA polymerase Rpb1, domain 6 / RNA polymerase Rpb1 C-terminal repeat / Eukaryotic RNA polymerase II heptapeptide repeat. / RNA polymerase Rpb1, domain 7 / RNA polymerase Rpb1, domain 7 superfamily / RNA polymerase Rpb1, domain 7 / VWFA domain profile. / Metallo-beta-lactamase superfamily / von Willebrand factor, type A / Metallo-beta-lactamase / von Willebrand factor A-like domain superfamily / Ribonuclease Z/Hydroxyacylglutathione hydrolase-like / RNA polymerase Rpb1, domain 3 superfamily / RNA polymerase Rpb1, clamp domain superfamily / RNA polymerase Rpb1, domain 3 / RNA polymerase Rpb1, domain 3 / DNA-directed RNA polymerase, subunit beta-prime / RNA polymerase Rpb1, domain 1 / RNA polymerase Rpb1, domain 1 / RNA polymerase, alpha subunit / RNA polymerase Rpb1, domain 5 / RNA polymerase Rpb1, domain 4 / RNA polymerase Rpb1, domain 2 / RNA polymerase Rpb1, domain 5 / RNA polymerase Rpb1, domain 4 / RNA polymerase, N-terminal / RNA polymerase Rpb1, funnel domain superfamily

Component:

DNA-directed RNA polymerase subunit / Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform / Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform / Integrator complex subunit 11 / Integrator complex subunit 3 / Integrator complex subunit 5 / Integrator complex subunit 8 / Integrator complex subunit 1 / Integrator complex subunit 4 / Integrator complex subunit 15 / Integrator complex subunit 14 / SOSS complex subunit B1 / Integrator complex subunit 2 / SOSS complex subunit C / Integrator complex subunit 9 / Integrator complex subunit 7 / Integrator complex subunit 13 / Integrator complex subunit 10 / Integrator complex subunit 6

• Biological species: Homo sapiens (human) / Sus scrofa (pig) / Trichoplusia ni (cabbage looper)
• Method: single particle reconstruction / cryo EM / Resolution: 3.7 Å
• Authors: Fianu I / Ochmann M / Walshe JL / Cramer P

Funding support

Germany, European Union, 3 items

Organization	Grant number	Country
German Research Foundation (DFG)	SFB860	Germany
German Research Foundation (DFG)	SFB1565	Germany
European Research Council (ERC)	EXC 2067/1-390729940	European Union

• Citation: Journal: Nature / Year: 2024; Title: Structural basis of Integrator-dependent RNA polymerase II termination.; Authors: Isaac Fianu / Moritz Ochmann / James L Walshe / Olexandr Dybkov / Joseph Neos Cruz / Henning Urlaub / Patrick Cramer / ; Abstract: The Integrator complex can terminate RNA polymerase II (Pol II) in the promoter-proximal region of genes. Previous work has shed light on how Integrator binds to the paused elongation complex consisting of Pol II, the DRB sensitivity-inducing factor (DSIF) and the negative elongation factor (NELF) and how it cleaves the nascent RNA transcript, but has not explained how Integrator removes Pol II from the DNA template. Here we present three cryo-electron microscopy structures of the complete Integrator-PP2A complex in different functional states. The structure of the pre-termination complex reveals a previously unresolved, scorpion-tail-shaped INTS10-INTS13-INTS14-INTS15 module that may use its 'sting' to open the DSIF DNA clamp and facilitate termination. The structure of the post-termination complex shows that the previously unresolved subunit INTS3 and associated sensor of single-stranded DNA complex (SOSS) factors prevent Pol II rebinding to Integrator after termination. The structure of the free Integrator-PP2A complex in an inactive closed conformation reveals that INTS6 blocks the PP2A phosphatase active site. These results lead to a model for how Integrator terminates Pol II transcription in three steps that involve major rearrangements.

History

Deposition	Dec 5, 2023	-
Header (metadata) release	Feb 7, 2024	-
Map release	Feb 7, 2024	-
Update	May 15, 2024	-
Current status	May 15, 2024	Processing site: PDBe / Status: Released

Map

• File: Download / File: emd_19040.map.gz / Format: CCP4 / Size: 421.9 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
• Annotation: Overall map filtered by local resolution
• Voxel size: X=Y=Z: 1.05 Å

Density

Contour Level	By AUTHOR: 0.00308
Minimum - Maximum	-0.08337648 - 0.19503137
Average (Standard dev.)	0.00030226208 (±0.0026171028)

• Symmetry: Space group: 1

Details

EMDB XML:

Map geometry	Axis order X Y Z Origin 0 0 0 Dimensions 480 480 480 Spacing 480 480 480
Cell	A=B=C: 503.99997 Å α=β=γ: 90.0 °

Supplemental data

Additional map: Focused refined map around Integrator cleavage module (INTS4,...

• File: emd_19040_additional_1.map
• Annotation: Focused refined map around Integrator cleavage module (INTS4, INTS9 and INTS11)

Additional map: Focused refined map around INTS1, INTS2 INTS7 and DSS1

• File: emd_19040_additional_2.map
• Annotation: Focused refined map around INTS1, INTS2 INTS7 and DSS1

Additional map: Focused refined map around INTS5, INTS8 and INTS15

• File: emd_19040_additional_3.map
• Annotation: Focused refined map around INTS5, INTS8 and INTS15

Additional map: Focused refined map around INTS6 and PP2A

• File: emd_19040_additional_4.map
• Annotation: Focused refined map around INTS6 and PP2A

Additional map: Focused refined map around INTS3 and SOSS factors

• File: emd_19040_additional_5.map
• Annotation: Focused refined map around INTS3 and SOSS factors

Additional map: Focused refined map around INTS1 c-terminus and center of Integrator

• File: emd_19040_additional_6.map
• Annotation: Focused refined map around INTS1 c-terminus and center of Integrator

Half map: #1

• File: emd_19040_half_map_1.map

Half map: #2

• File: emd_19040_half_map_2.map

Sample components

Entire : Post-termination complex

• Entire: Name: Post-termination complex

Components

• Complex: Post-termination complex
  • Complex: Integrator-PP2A complex and NABP2 and INIP
    • Protein or peptide: UNK-UNK-UNK-UNK-UNK-UNK-UNK-UNK-UNK
    • Protein or peptide: DNA-directed RNA polymerase subunit
  • Complex: RNA Polymerase II C-terminal domain peptides
    • Protein or peptide: SOSS complex subunit B1
    • Protein or peptide: SOSS complex subunit C
    • Protein or peptide: Integrator complex subunit 1
    • Protein or peptide: Integrator complex subunit 2
    • Protein or peptide: Integrator complex subunit 3
    • Protein or peptide: Integrator complex subunit 4
    • Protein or peptide: Integrator complex subunit 5
    • Protein or peptide: Integrator complex subunit 6
    • Protein or peptide: Integrator complex subunit 7
    • Protein or peptide: Integrator complex subunit 8
    • Protein or peptide: Integrator complex subunit 9
    • Protein or peptide: Integrator complex subunit 10
    • Protein or peptide: Integrator complex subunit 11
    • Protein or peptide: Integrator complex subunit 14
    • Protein or peptide: Integrator complex subunit 15
    • Protein or peptide: Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform
    • Protein or peptide: Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform
    • Protein or peptide: Integrator complex subunit 13
  • Complex: DSS1
    • Protein or peptide: DSS1
• Ligand: ZINC ION
• Ligand: MANGANESE (II) ION

Supramolecule #1: Post-termination complex

• Supramolecule: Name: Post-termination complex / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#21; Details: Integrator-PP2A, RNA Polymerase II C-terminal domain peptides, NABP2, INIP
• Molecular weight: Theoretical: 1.2 MDa

Supramolecule #2: Integrator-PP2A complex and NABP2 and INIP

• Supramolecule: Name: Integrator-PP2A complex and NABP2 and INIP / type: complex / ID: 2 / Parent: 1 / Macromolecule list: #1, #4
• Source (natural): Organism: Homo sapiens (human)

Supramolecule #3: RNA Polymerase II C-terminal domain peptides

• Supramolecule: Name: RNA Polymerase II C-terminal domain peptides / type: complex / ID: 3 / Parent: 1 / Macromolecule list: #2-#3, #5-#19, #21
• Source (natural): Organism: Sus scrofa (pig)

Supramolecule #4: DSS1

• Supramolecule: Name: DSS1 / type: complex / ID: 4 / Parent: 1 / Macromolecule list: #20
• Source (natural): Organism: Trichoplusia ni (cabbage looper)

Macromolecule #1: UNK-UNK-UNK-UNK-UNK-UNK-UNK-UNK-UNK

• Macromolecule: Name: UNK-UNK-UNK-UNK-UNK-UNK-UNK-UNK-UNK / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Sus scrofa (pig)
• Molecular weight: Theoretical: 783.958 Da

Sequence

String:

(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)

Macromolecule #2: SOSS complex subunit B1

• Macromolecule: Name: SOSS complex subunit B1 / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 22.566053 KDa
• Recombinant expression: Organism: Trichoplusia ni (cabbage looper)

Sequence

String:

SNMTTETFVK DIKPGLKNLN LIFIVLETGR VTKTKDGHEV RTCKVADKTG SINISVWDDV GNLIQPGDII RLTKGYASVF KGCLTLYTG RGGDLQKIGE FCMVYSEVPN FSEPNPEYST QQAPNKAVQN DSNPSASQPT TGPSAASPAS ENQNGNGLSA P PGPGGGPH PPHTPSHPPS TRITRSQPNH TPAGPPGPSS NPVSNGKETR RSSKR

UniProtKB: SOSS complex subunit B1

Macromolecule #3: SOSS complex subunit C

• Macromolecule: Name: SOSS complex subunit C / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 11.642977 KDa
• Recombinant expression: Organism: Trichoplusia ni (cabbage looper)

Sequence

String:

SNMAANSSGQ GFQNKNRVAI LAELDKEKRK LLMQNQSSTN HPGASIALSR PSLNKDFRDH AEQQHIAAQQ KAALQHAHAH SSGYFITQD SAFGNLILPV LPRLDPE

UniProtKB: SOSS complex subunit C

Macromolecule #4: DNA-directed RNA polymerase subunit

• Macromolecule: Name: DNA-directed RNA polymerase subunit / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO / EC number: DNA-directed RNA polymerase
• Source (natural): Organism: Sus scrofa (pig)
• Molecular weight: Theoretical: 1.356393 KDa

Sequence

String:

SPSYSPTSPS YSP

UniProtKB: DNA-directed RNA polymerase subunit

Macromolecule #5: Integrator complex subunit 1

• Macromolecule: Name: Integrator complex subunit 1 / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 244.776078 KDa
• Recombinant expression: Organism: Trichoplusia ni (cabbage looper)

Sequence

String:

SNMNRAKPTT VRRPSAAAKP SGHPPPGDFI ALGSKGQANE SKTASTLLKP APSGLPSERK RDAAAALSSA SALTGLTKRP KLSSTPPLS ALGRLAEAAV AEKRAISPSI KEPSVVPIEV LPTVLLDEIE AAELEGNDDR IEGVLCGAVK QLKVTRAKPD S TLYLSLMY LAKIKPNIFA TEGVIEALCS LLRRDASINF KAKGNSLVSV LACNLLMAAY EEDENWPEIF VKVYIEDSLG ER IWVDSPH CKTFVDNIQT AFNTRMPPRS VLLQGEAGRV AGDLGAGSSP HPSLTEEEDS QTELLIAEEK LSPEQEGQLM PRY EELAES VEEYVLDMLR DQLNRRQPID NVSRNLLRLL TSTCGYKEVR LLAVQKLEMW LQNPKLTRPA QDLLMSVCMN CNTH GSEDM DVISHLIKIR LKPKVLLNHF MLCIRELLSA HKDNLGTTIK LVIFNELSSA RNPNNMQVLY TALQHSSELA PKFLA MVFQ DLLTNKDDYL RASRALLREI IKQTKHEINF QAFCLGLMQE RKEPQYLEME FKERFVVHIT DVLAVSMMLG ITAQVK EAG IAWDKGEKRN LEVLRSFQNQ IAAIQRDAVW WLHTVVPSIS KLAPKDYVHC LHKVLFTEQP ETYYKWDNWP PESDRNF FL RLCSEVPILE DTLMRILVIG LSRELPLGPA DAMELADHLV KRAAAVQADD VEVLKVGRTQ LIDAVLNLCT YHHPENIQ L PPGYQPPNLA ISTLYWKAWP LLLVVAAFNP ENIGLAAWEE YPTLKMLMEM VMTNNYSYPP CTLTDEETRT EMLNRELQT AQREKQEILA FEGHLAAAST KQTITESSSL LLSQLTSLDP QGPPRRPPPH ILDQVKSLNQ SLRLGHLLCR SRNPDFLLHI IQRQASSQS MPWLADLVQS SEGSLDVLPV QCLCEFLLHD AVDDAASGEE DDEGESKEQK AKKRQRQQKQ RQLLGRLQDL L LGPKADEQ TTCEVLDYFL RRLGSSQVAS RVLAMKGLSL VLSEGSLRDG EEKEPPMEED VGDTDVLQGY QWLLRDLPRL PL FDSVRST TALALQQAIH METDPQTISA YLIYLSQHTP VEEQAQHSDL ALDVARLVVE RSTIMSHLFS KLSPSAASDA VLS ALLSIF SRYVRRMRQS KEGEEVYSWS ESQDQVFLRW SSGETATMHI LVVHAMVILL TLGPPRADDS EFQALLDIWF PEEK PLPTA FLVDTSEEAL LLPDWLKLRM IRSEVLRLVD AALQDLEPQQ LLLFVQSFGI PVSSMSKLLQ FLDQAVAHDP QTLEQ NIMD KNYMAHLVEV QHERGASGGQ TFHSLLTASL PPRRDSTEAP KPKSSPEQPI GQGRIRVGTQ LRVLGPEDDL AGMFLQ IFP LSPDPRWQSS SPRPVALALQ QALGQELARV VQGSPEVPGI TVRVLQALAT LLSSPHGGAL VMSMHRSHFL ACPLLRQ LC QYQRCVPQDT GFSSLFLKVL LQMLQWLDSP GVEGGPLRAQ LRMLASQASA GRRLSDVRGG LLRLAEALAF RQDLEVVS S TVRAVIATLR SGEQCSVEPD LISKVLQGLI EVRSPHLEEL LTAFFSATAD AASPFPACKP VVVVSSLLLQ EEEPLAGGK PGADGGSLEA VRLGPSSGLL VDWLEMLDPE VVSSCPDLQL RLLFSRRKGK GQAQVPSFRP YLLTLFTHQS SWPTLHQCIR VLLGKSREQ RFDPSASLDF LWACIHVPRI WQGRDQRTPQ KRREELVLRV QGPELISLVE LILAEAETRS QDGDTAACSL I QARLPLLL SCCCGDDESV RKVTEHLSGC IQQWGDSVLG RRCRDLLLQL YLQRPELRVP VPEVLLHSEG AASSSVCKLD GL IHRFITL LADTSDSRAL ENRGADASMA CRKLAVAHPL LLLRHLPMIA ALLHGRTHLN FQEFRQQNHL SCFLHVLGLL ELL QPHVFR SEHQGALWDC LLSFIRLLLN YRKSSRHLAA FINKFVQFIH KYITYNAPAA ISFLQKHADP LHDLSFDNSD LVML KSLLA GLSLPSRDDR TDRGLDEEGE EESSAGSLPL VSVSLFTPLT AAEMAPYMKR LSRGQTVEDL LEVLSDIDEM SRRRP EILS FFSTNLQRLM SSAEECCRNL AFSLALRSMQ NSPSIAAAFL PTFMYCLGSQ DFEVVQTALR NLPEYALLCQ EHAAVL LHR AFLVGMYGQM DPSAQISEAL RILHMEAVM

UniProtKB: Integrator complex subunit 1

Macromolecule #6: Integrator complex subunit 2

• Macromolecule: Name: Integrator complex subunit 2 / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 134.451625 KDa
• Recombinant expression: Organism: Trichoplusia ni (cabbage looper)

Sequence

String:

MKDQQTVIMT ECTSLQFVSP FAFEAMQKVD VVCLASLSDP ELRLLLPCLV RMALCAPADQ SQSWAQDKKL ILRLLSGVEA VNSIVALLS VDFHALEQDA SKEQQLRHKL GGGSGESILV SQLQHGLTLE FEHSDSPRRL RLVLSELLAI MNKVSESNGE F FFKSSELF ESPVYLEEAA DVLCILQAEL PSLLPIVDVA EALLHVRNGA WFLCLLVANV PDSFNEVCRG LIKNGERQDE ES LGGRRRT DALRFLCKMN PSQALKVRGM VVEECHLPGL GVALTLDHTK NEACEDGVSD LVCFVSGLLL GTNAKVRTWF GTF IRNGQQ RKRETSSSVL WQMRRQLLLE LMGILPTVRS TRIVEEADVD MEPNVSVYSG LKEEHVVKAS ALLRLYCALM GIAG LKPTE EEAEQLLQLM TSRPPATPAG VRFVSLSFCM LLAFSTLVST PEQEQLMVVW LSWMIKEEAY FESTSGVSAS FGEML LLVA MYFHSNQLSA IIDLVCSTLG MKIVIKPSSL SRMKTIFTQE IFTEQVVTAH AVRVPVTSNL SANITGFLPI HCIYQL LRS RSFTKHKVSI KDWIYRQLCE TSTPLHPQLL PLIDVYINSI LTPASKSNPE ATNQPVTEQE ILNIFQGVIG GDNIRLN QR FSITAQLLVL YYILSYEEAL LANTKTLAAM QRKPKSYSSS LMDQIPIKFL IRQAQGLQQE LGGLHSALLR LLATNYPH L CIVDDWICEE EITGTDALLR RMLLTNNAKN HSPKQLQEAF SAVPVNNTQV MQIIEHLTLL SASELIPYAE VLTSNMSQL LNSGVPRRIL QTVNKLWMVL NTVMPRRLWV MTVNALQPSI KFVRQQKYTQ NDLMIDPLIV LRCDQRVHRC PPLMDITLHM LNGYLLASK AYLSAHLKET EQDRPSQNNT IGLVGQTDAP EVTREELKNA LLAAQDSAAV QILLEICLPT EEEKANGVNP D SLLRNVQS VITTSAPNKG MEEGEDNLLC NLREVQCLIC CLLHQMYIAD PNIAKLVHFQ GYPCELLPLT VAGIPSMHIC LD FIPELIA QPELEKQIFA IQLLSHLCIQ YALPKSLSVA RLAVNVMGTL LTVLTQAKRY AFFMPTLPSL VSFCRAFPPL YED IMSLLI QIGQVCASDV ATQTRDIDPI ITRLQQIKEK PSGWSQICKD SSYKNGSRDT GSMDPDVQLC HCIERTVIEI INMS VSGI

UniProtKB: Integrator complex subunit 2

Macromolecule #7: Integrator complex subunit 3

• Macromolecule: Name: Integrator complex subunit 3 / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 118.155555 KDa
• Recombinant expression: Organism: Trichoplusia ni (cabbage looper)

Sequence

String:

MELQKGKGAA AAAAASGAAG GGGGGAGAGA PGGGRLLLST SLDAKDELEE RLERCMSIVT SMTAGVSERE ANDALNAYVC KGLPQHEEI CLGLFTLILT EPAQAQKCYR DLALVSRDGM NIVLNKINQI LMEKYLKLQD TCRTQLVWLV RELVKSGVLG A DGVCMTFM KQIAGGDVTA KNIWLAESVL DILTEQREWV LKSSILIAMA VYTYLRLIVD HHGTAQLQAL RQKEVDFCIS LL RERFMEC LMIGRDLVRL LQNVARIPEF ELLWKDIIHN PQALSPQFTG ILQLLQSRTS RKFLACRLTP DMETKLLFMT SRV RFGQQK RYQDWFQRQY LSTPDSQSLR CDLIRYICGV VHPSNEVLSS DILPRWAIIG WLLTTCTSNV AASNAKLALF YDWL FFSPD KDSIMNIEPA ILVMHHSMKP HPAITATLLD FMCRIIPNFY PPLEGHVRQG VFSSLNHIVE KRVLAHLAPL FDNPK LDKE LRAMLREKFP EFCSSPSPPV EVKIEEPVSM EMDNHMSDKD ESCYDNAEAA FSDDEEDLNS KGKKREFRFH PIKETV VEE PVDITPYLDQ LDESLRDKVL QLQKGSDTEA QCEVMQEIVD QVLEEDFDSE QLSVLASCLQ ELFKAHFRGE VLPEEIT EE SLEESVGKPL YLIFRNLCQM QEDNSSFSLL LDLLSELYQK QPKIGYHLLY YLRASKAAAG KMNLYESFAQ ATQLGDLH T CLMMDMKACQ EDDVRLLCHL TPSIYTEFPD ETLRSGELLN MIVAVIDSAQ LQELVCHVMM GNLVMFRKDS VLNILIQSL DWETFEQYCA WQLFLAHNIP LETIIPILQH LKYKEHPEAL SCLLLQLRRE KPSEEMVKMV LSRPCHPDDQ FTTSILRHWC MKHDELLAE HIKSLLIKNN SLPRKRQSLR SSSSKLAQLT LEQILEHLDN LRLNLTNTKQ NFFSQTPILQ ALQHVQASCD E AHKMKFSD LFSLAEEYED SSTKPPKSRR KAALSSPRSR KNATQPPNAE EESGSSSASE EEDTKPKPTK RKRKGSSAVG SD SD

UniProtKB: Integrator complex subunit 3

Macromolecule #8: Integrator complex subunit 4

• Macromolecule: Name: Integrator complex subunit 4 / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 108.306758 KDa
• Recombinant expression: Organism: Trichoplusia ni (cabbage looper)

Sequence

String:

MAAHLKKRVY EEFTKVVQPQ EEIATKKLRL TKPSKSAALH IDLCKATSPA DALQYLLQFA RKPVEAESVE GVVRILLEHY YKENDPSVR LKIASLLGLL SKTAGFSPDC IMDDAINILQ NEKSHQVLAQ LLDTLLAIGT KLPENQAIQM RLVDVACKHL T DTSHGVRN KCLQLLGNLG SLEKSVTKDA EGLAARDVQK IIGDYFSDQD PRVRTAAIKA MLQLHERGLK LHQTIYNQAC KL LSDDYEQ VRSAAVQLIW VVSQLYPESI VPIPSSNEEI RLVDDAFGKI CHMVSDGSWV VRVQAAKLLG SMEQVSSHFL EQT LDKKLM SDLRRKRTAH ERAKELYSSG EFSSGRKWGD DAPKEEVDTG AVNLIESGAC GAFVHGLEDE MYEVRIAAVE ALCM LAQSS PSFAEKCLDF LVDMFNDEIE EVRLQSIHTM RKISNNITLR EDQLDTVLAV LEDSSRDIRE ALHELLCCTN VSTKE GIHL ALVELLKNLT KYPTDRDSIW KCLKFLGSRH PTLVLPLVPE LLSTHPFFDT AEPDMDDPAY IAVLVLIFNA AKTCPT MPA LFSDHTFRHY AYLRDSLSHL VPALRLPGRK LVSSAVSPSI IPQEDPSQQF LQQSLERVYS LQHLDPQGAQ ELLEFTI RD LQRLGELQSE LAGVADFSAT YLRCQLLLIK ALQEKLWNVA APLYLKQSDL ASAAAKQIME ETYKMEFMYS GVENKQVV I IHHMRLQAKA LQLIVTARTT RGLDPLFGMC EKFLQEVDFF QRYFIADLPH LQDSFVDKLL DLMPRLMTSK PAEVVKILQ TMLRQSAFLH LPLPEQIHKA SATIIEPAGE SDNPLRFTSG LVVALDVDAT LEHVQDPQNT VKVQVLYPDG QAQMIHPKPA DFRNPGPGR HRLITQVYLS HTAWTEACQV EVRLLLAYNS SARIPKCPWM EGGEMSPQVE TSIEGTIPFS KPVKVYIMPK P ARR

UniProtKB: Integrator complex subunit 4

Macromolecule #9: Integrator complex subunit 5

• Macromolecule: Name: Integrator complex subunit 5 / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 108.316422 KDa
• Recombinant expression: Organism: Trichoplusia (butterflies/moths)

Sequence

String:

SNMSALCDPP GAPGPPGPAP ATHGPAPLSA QELSQEIKAF LTGVDPILGH QLSAREHARC GLLLLRSLPP ARAAVLDHLR GVFDESVRA HLAALDETPV AGPPHLRPPP PSHVPAGGPG LEDVVQEVQQ VLSEFIRANP KAWAPVISAW SIDLMGQLSS T YSGQHQRV PHATGALNEL LQLWMGCRAT RTLMDIYVQC LSALIGSCPD ACVDALLDTS VQHSPHFDWV VAHIGSSFPG TI ISRVLSC GLKDFCVHGG AGGGAGSSGG SSSQTPSTDP FPGSPAIPAE KRVPKIASVV GILGHLASRH GDSIRRELLR MFH DSLAGG SGGRSGDPSL QATVPFLLQL AVMSPALLGT VSGELVDCLK PPAVLSQLQQ HLQGFPREEL DNMLNLAVHL VSQA SGAGA YRLLQFLVDT AMPASVITTQ GLAVPDTVRE ACDRLIQLLL LHLQKLVHHR GGSPGEGVLG PPPPPRLVPF LDALK NHVG ELCGETLRLE RKRFLWQHQL LGLLSVYTRP SCGPEALGHL LSRARSPEEL SLATQLYAGL VVSLSGLLPL AFRSCL ARV HAGTLQPPFT ARFLRNLALL VGWEQQGGEG PAALGAHFGE SASAHLSDLA PLLLHPEEEV AEAAASLLAI CPFPSEA LS PSQLLGLVRA GVHRFFASLR LHGPPGVASA CQLLTRLSQT SPAGLKAVLQ LLVEGALHRG NTELFGGQVD GDNETLSV V SASLASASLL DTNRRHTAAV PGPGGIWSVF HAGVIGRGLK PPKFVQSRNQ QEVIYNTQSL LSLLVHCCSA PGGTECGEC WGAPILSPEA AKAVAVTLVE SVCPDAAGAE LAWPPEEHAR ATVERDLRIG RRFREQPLLF ELLKLVAAAP PALCYCSVLL RGLLAALLG HWEASRHPDT THSPWHLEAS CTLVAVMAEG SLLPPALGNM HEVFSQLAPF EVRLLLLSVW GFLREHGPLP Q KFIFQSER GRFIRDFSRE GGGEGGPHLA VLHSVLHRNI DRLGLFSGRF QAPSPSTLLR QGT

UniProtKB: Integrator complex subunit 5

Macromolecule #10: Integrator complex subunit 6

• Macromolecule: Name: Integrator complex subunit 6 / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 100.72825 KDa
• Recombinant expression: Organism: Trichoplusia ni (cabbage looper)

Sequence

String:

SNMPILLFLI DTSASMNQRS HLGTTYLDTA KGAVETFMKL RARDPASRGD RYMLVTFEEP PYAIKAGWKE NHATFMNELK NLQAEGLTT LGQSLRTAFD LLNLNRLVTG IDNYGQGRNP FFLEPAIIIT ITDGSKLTTT SGVQDELHLP LNSPLPGSEL T KEPFRWDQ RLFALVLRLP GTMSVESEQL TGVPLDDSAI TPMCEVTGGR SYSVCSPRML NQCLESLVQK VQSGVVINFE KA GPDPSPV EDGQPDISRP FGSQPWHSCH KLIYVRPNPK TGVPIGHWPV PESFWPDQNS PTLPPRTSHP VVKFSCTDCE PMV IDKLPF DKYELEPSPL TQFILERKSP QTCWQVYVSN SAKYSELGHP FGYLKASTAL NCVNLFVMPY NYPVLLPLLD DLFK VHKAK PTLKWRQSFE SYLKTMPPYY LGPLKKAVRM MGAPNLIADS MEYGLSYSVI SYLKKLSQQA KIESDRVIGS VGKKV VQET GIKVRSRSHG LSMAYRKDFQ QLLQGISEDV PHRLLDLNMK EYTGFQVALL NKDLKPQTFR NAYDIPRRNL LDHLTR MRS NLLKSTRRFL KGQDEDQVHS VPIAQMGNYQ EYLKQVPSPL RELDPDQPRR LHTFGNPFKL DKKGMMIDEA DEFVAGP QN KHKRPGEPNM QGIPKRRRCM SPLLRGRQQN PVVNNHIGGK GPPAPTTQAQ PDLIKPLPLH KISETTNDSI IHDVVENH V ADQLSSDITP NAMDTEFSAS SPASLLERPT NHMEALGHDH LGTNDLTVGG FLENHEEPRD KEQCAEENIP ASSLNKGKK LMHCRSHEEV NTELKAQIMK EIRKPGRKYE RIFTLLKHVQ GSLQTRLIFL QNVIKEASRF KKRMLIEQLE NFLDEIHRRA NQINHINSN

UniProtKB: Integrator complex subunit 6

Macromolecule #11: Integrator complex subunit 7

• Macromolecule: Name: Integrator complex subunit 7 / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 107.153805 KDa
• Recombinant expression: Organism: Trichoplusia ni (cabbage looper)

Sequence

String:

SNMASNSTKS FLADAGYGEQ ELDANSALME LDKGLRSGKL GEQCEAVVRF PRLFQKYPFP ILINSAFLKL ADVFRVGNNF LRLCVLKVT QQSEKHLEKI LNVDEFVKRI FSVIHSNDPV ARAITLRMLG SLASIIPERK NAHHSIRQSL DSHDNVEVEA A VFAAANFS AQSKDFAVGI CNKISEMIQG LATPVDLKLK LIPILQHMHH DAILASSARQ LLQQLVTSYP STKMVIVSLH TF TLLAASS LVDTPKQIQL LLQYLKNDPR KAVKRLAIQD LKLLANKTPH TWSRENIQAL CECALQTPYD SLKLGMLSVL STL SGTIAI KHYFSIVPGN VSSSPRSSDL VKLAQECCYH NNRGIAAHGV RVLTNITVSC QEKDLLALEQ DAVFGLESLL VLCS QDDSP GAQATLKIAL NCMVKLAKGR PHLSQSVVET LLTQLHSAQD AARILMCHCL AAIAMQLPVL GDGMLGDLME LYKVI GRSA TDKQQELLVS LATVIFVASQ KALSVESKAV IKQQLESVSN GWTVYRIARQ ASRMGNHDMA KELYQSLLTQ VASEHF YFW LNSLKEFSHA EQCLTGLQEE NYSSALSCIA ESLKFYHKGI ASLTAASTPL NPLSFQCEFV KLRIDLLQAF SQLICTC NS LKTSPPPAIA TTIAMTLGND LQRCGRISNQ MKQSMEEFRS LASRYGDLYQ ASFDADSATL RNVELQQQSC LLISHAIE A LILDPESASF QEYGSTGTAH ADSEYERRMM SVYNHVLEEV ESLNRKYTPV SYMHTACLCN AIIALLKVPL SFQRYFFQK LQSTSIKLAL SPSPRNPAEP IAVQNNQQLA LKVEGVVQHG SKPGLFRKIQ SVCLNVSSTL QSKSGQDYKI PIDNMTNEME QRVEPHNDY FSTQFLLNFA ILGTHNITVE SSVKDANGIV WKTGPRTTIF VKSLEDPYSQ QIRLQQQQAQ QPLQQQQQRN A YTRF

UniProtKB: Integrator complex subunit 7

Macromolecule #12: Integrator complex subunit 8

• Macromolecule: Name: Integrator complex subunit 8 / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 113.219859 KDa
• Recombinant expression: Organism: Trichoplusia ni (cabbage looper)

Sequence

String:

MSAEAADREA ATSSRPCTPP QTCWFEFLLE ESLLEKHLRK PCPDPAPVQL IVQFLEQASK PSVNEQNQVQ PPPDNKRNRI LKLLALKVA AHLKWDLDIL EKSLSVPVLN MLLNELLCIS KVPPGTKHVD MDLATLPPTT AMAVLLYNRW AIRTIVQSSF P VKQAKPGP PQLSVMNQMQ QEKELTENIL KVLKEQAADS ILVLEAALKL NKDLYVHTMR TLDLLAMEPG MVNGETESST AG LKVKTEE MQCQVCYDLG AAYFQQGSTN SAVYENAREK FFRTKELIAE IGSLSLHCTI DEKRLAGYCQ ACDVLVPSSD STS QQLTPY SQVHICLRSG NYQEVIQIFI EDNLTLSLPV QFRQSVLREL FKKAQQGNEA LDEICFKVCA CNTVRDILEG RTIS VQFNQ LFLRPNKEKI DFLLEVCSRS VNLEKASESL KGNMAAFLKN VCLGLEDLQY VFMISSHELF ITLLKDEERK LLVDQ MRKR SPRVNLCIKP VTSFYDIPAS ASVNIGQLEH QLILSVDPWR IRQILIELHG MTSERQFWTV SNKWEVPSVY SGVILG IKD NLTRDLVYIL MAKGLHCSTV KDFSHAKQLF AACLELVTEF SPKLRQVMLN EMLLLDIHTH EAGTGQAGER PPSDLIS RV RGYLEMRLPD IPLRQVIAEE CVAFMLNWRE NEYLTLQVPA FLLQSNPYVK LGQLLAATCK ELPGPKESRR TAKDLWEV V VQICSVSSQH KRGNDGRVSL IKQRESTLGI MYRSELLSFI KKLREPLVLT IILSLFVKLH NVREDIVNDI TAEHISIWP SSIPNLQSVD FEAVAITVKE LVRYTLSINP NNHSWLIIQA DIYFATNQYS AALHYYLQAG AVCSDFFNKA VPPDVYTDQV IKRMIKCCS LLNCHTQVAI LCQFLREIDY KTAFKSLQEQ NSHDAMDSYY DYIWDVTILE YLTYLHHKRG ETDKRQIAIK A IGQTELNA SNPEEVLQLA AQRRKKKFLQ AMAKLYF

UniProtKB: Integrator complex subunit 8

Macromolecule #13: Integrator complex subunit 9

• Macromolecule: Name: Integrator complex subunit 9 / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 73.891219 KDa
• Recombinant expression: Organism: Trichoplusia ni (cabbage looper)

Sequence

String:

MKLYCLSGHP TLPCNVLKFK STTIMLDCGL DMTSTLNFLP LPLVQSPRLS NLPGWSLKDG NAFLDKELKE CSGHVFVDSV PEFCLPETE LIDLSTVDVI LISNYHCMMA LPYITEHTGF TGTVYATEPT VQIGRLLMEE LVNFIERVPK AQSASLWKNK D IQRLLPSP LKDAVEVSTW RRCYTMQEVN SALSKIQLVG YSQKIELFGA VQVTPLSSGY ALGSSNWIIQ SHYEKVSYVS GS SLLTTHP QPMDQASLKN SDVLVLTGLT QIPTANPDGM VGEFCSNLAL TVRNGGNVLV PCYPSGVIYD LLECLYQYID SAG LSSVPL YFISPVANSS LEFSQIFAEW LCHNKQSKVY LPEPPFPHAE LIQTNKLKHY PSIHGDFSND FRQPCVVFTG HPSL RFGDV VHFMELWGKS SLNTVIFTEP DFSYLEALAP YQPLAMKCIY CPIDTRLNFI QVSKLLKEVQ PLHVVCPEQY TQPPP AQSH RMDLMIDCQP PAMSYRRAEV LALPFKRRYE KIEIMPELAD SLVPMEIKPG ISLATVSAVL HTKDNKHLLQ PPPRPA QPT SGKKRKRVSD DVPDCKVLKP LLSGSIPVEQ FVQTLEKHGF SDIKVEDTAK GHIVLLQEAE TLIQIEEDST HIICDND EM LRVRLRDLVL KFLQKF

UniProtKB: Integrator complex subunit 9

Macromolecule #14: Integrator complex subunit 10

• Macromolecule: Name: Integrator complex subunit 10 / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 82.339867 KDa
• Recombinant expression: Organism: Trichoplusia ni (cabbage looper)

Sequence

String:

MSAQGDCEFL VQRARELVPQ DLWAAKAWLI TARSLYPADF NIQYEMYTIE RNAERTATAG RLLYDMFVNF PDQPVVWREI SIITSALRN DSQDKQTQFL RSLFETLPGR VQCEMLLKVT EQCFNTLERS EMLLLLLRRF PETVVQHGVG LGEALLEAET I EEQESPVN CFRKLFVCDV LPLIINNHDV RLPANLLYKY LNKAAEFYIN YVTRSTQIEN QHQGAQDTSD LMSPSKRSSQ KY IIEGLTE KSSQIVDPWE RLFKILNVVG MRCEWQMDKG RRSYGDILHR MKDLCRYMNN FDSEAHAKYK NQVVYSTMLV FFK NAFQYV NSIQPSLFQG PNAPSQVPLV LLEDVSNVYG DVEIDRNKHI HKKRKLAEGR EKTMSSDDED CSAKGRNRHI VVNK AELAN STEVLESFKL ARESWELLYS LEFLDKEFTR ICLAWKTDTW LWLRIFLTDM IIYQGQYKKA IASLHHLAAL QGSIS QPQI TGQGTLEHQR ALIQLATCHF ALGEYRMTCE KVLDLMCYMV LPIQDGGKSQ EEPSKVKPKF RKGSDLKLLP CTSKAI MPY CLHLMLACFK LRAFTDNRDD MALGHVIVLL QQEWPRGENL FLKAVNKICQ QGNFQYENFF NYVTNIDMLE EFAYLRT QE GGKIHLELLP NQGMLIKHHT VTRGITKGVK EDFRLAMERQ VSRCGENLMV VLHRFCINEK ILLLQTLT

UniProtKB: Integrator complex subunit 10

Macromolecule #15: Integrator complex subunit 11

• Macromolecule: Name: Integrator complex subunit 11 / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 67.970781 KDa
• Recombinant expression: Organism: Trichoplusia ni (cabbage looper)

Sequence

String:

SNMPEIRVTP LGAGQDVGRS CILVSIAGKN VMLDCGMHMG FNDDRRFPDF SYITQNGRLT DFLDCVIISH FHLDHCGALP YFSEMVGYD GPIYMTHPTQ AICPILLEDY RKIAVDKKGE ANFFTSQMIK DCMKKVVAVH LHQTVQVDDE LEIKAYYAGH V LGAAMFQI KVGSESVVYT GDYNMTPDRH LGAAWIDKCR PNLLITQSTY ATTIRDSKRC RERDFLKKVH ETVERGGKVL IP VFALGRA QELCILLETF WERMNLKVPI YFSTGLTEKA NHYYKLFIPW TNQKIRKTFV QRNMFEFKHI KAFDRAFADN PGP MVVFAT PGMLHAGQSL QIFRKWAGNE KNMVIMPGYC VQGTVGHKIL SGQRKLEMEG RQVLEVKMQV EYMSFSAHAD AKGI MQLVG QAEPESVLLV HGEAKKMEFL KQKIEQELRV NCYMPANGET VTLPTSPSIP VGISLGLLKR EMAQGLLPEA KKPRL LHGT LIMKESNFRL VSSEQALKEL GLAEHQLRFT CRVHLHDTRK EQETALRVYS HLKSVLKDHC VQHLPDGSVT VESVLL QAA APSEDPGTKV LLVSWTYQDE ELGSFLTSLL KKGLPQAPS

UniProtKB: Integrator complex subunit 11

Macromolecule #16: Integrator complex subunit 14

• Macromolecule: Name: Integrator complex subunit 14 / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 57.526547 KDa
• Recombinant expression: Organism: Trichoplusia ni (cabbage looper)

Sequence

String:

MPTVVVMDVS LSMTRPVSIE GSEEYQRKHL AAHGLTMLFE HMATNYKLEF TALVVFSSLW ELMVPFTRDY NTLQEALSNM DDYDKTCLE SALVGVCNIV QQEWGGAIPC QVVLVTDGCL GIGRGSLRHS LATQNQRSES NRFPLPFPFP SKLYIMCMAN L EELQSTDS LECLERLIDL NNGEGQIFTI DGPLCLKNVQ SMFGKLIDLA YTPFHAVLKC GHLTADVQVF PRPEPFVVDE EI DPIPKVI NTDLEIVGFI DIADISSPPV LSRHLVLPIA LNKEGDEVGT GITDDNEDEN SANQIAGKIP NFCVLLHGSL KVE GMVAIV QLGPEWHGML YSQADSKKKS NLMMSLFEPG PEPLPWLGKM AQLGPISDAK ENPYGEDDNK SPFPLQPKNK RSYA QNVTV WIKPSGLQTD VQKILRNARK LPEKTQTFYK ELNRLRKAAL AFGFLDLLKG VADMLERECT LLPETAHPDA AFQLT HAAQ QLKLASTGTS EYAAYDQNIT PLHTDFSGSS TERI

UniProtKB: Integrator complex subunit 14

Macromolecule #17: Integrator complex subunit 15

• Macromolecule: Name: Integrator complex subunit 15 / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 50.303582 KDa
• Recombinant expression: Organism: Trichoplusia ni (cabbage looper)

Sequence

String:

SNMSDIRHSL LRRDALSAAK EVLYHLDIYF SSQLQSAPLP IVDKGPVELL EEFVFQVPKE RSAQPKRLNS LQELQLLEIM CNYFQEQTK DSVRQIIFSS LFSPQGNKAD DSRMSLLGKL VSMAVAVCRI PVLECAASWL QRTPVVYCVR LAKALVDDYC C LVPGSIQT LKQIFSASPR FCCQFITSVT ALYDLSSDDL IPPMDLLEMI VTWIFEDPRL ILITFLNTPI AANLPIGFLE LT PLVGLIR WCVKAPLAYK RKKKPPLSNG HVSNKVTKDP GVGMDRDSHL LYSKLHLSVL QVLMTLQLHL TEKNLYGRLG LIL FDHMVP LVEEINRLAD ELNPLNASQE IELSLDRLAQ ALQVAMASGA LLCTRDDLRT LCSRLPHNNL LQLVISGPVQ QSPH AALPP GFYPHIHTPP LGYGAVPAHP AAHPALPTHP GHTFISGVTF PFRPIR

UniProtKB: Integrator complex subunit 15

Macromolecule #18: Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit...

• Macromolecule: Name: Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform; type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 65.579531 KDa
• Recombinant expression: Organism: Trichoplusia ni (cabbage looper)

Sequence

String:

SNMAAADGDD SLYPIAVLID ELRNEDVQLR LNSIKKLSTI ALALGVERTR SELLPFLTDT IYDEDEVLLA LAEQLGTFTT LVGGPEYVH CLLPPLESLA TVEETVVRDK AVESLRAISH EHSPSDLEAH FVPLVKRLAG GDWFTSRTSA CGLFSVCYPR V SSAVKAEL RQYFRNLCSD DTPMVRRAAA SKLGEFAKVL ELDNVKSEII PMFSNLASDE QDSVRLLAVE ACVNIAQLLP QE DLEALVM PTLRQAAEDK SWRVRYMVAD KFTELQKAVG PEITKTDLVP AFQNLMKDCE AEVRAAASHK VKEFCENLSA DCR ENVIMS QILPCIKELV SDANQHVKSA LASVIMGLSP ILGKDNTIEH LLPLFLAQLK DECPEVRLNI ISNLDCVNEV IGIR QLSQS LLPAIVELAE DAKWRVRLAI IEYMPLLAGQ LGVEFFDEKL NSLCMAWLVD HVYAIREAAT SNLKKLVEKF GKEWA HATI IPKVLAMSGD PNYLHRMTTL FCINVLSEVC GQDITTKHML PTVLRMAGDP VANVRFNVAK SLQKIGPILD NSTLQS EVK PILEKLTQDQ DVDVKYFAQE ALTVLSLA

UniProtKB: Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform

Macromolecule #19: Serine/threonine-protein phosphatase 2A catalytic subunit alpha i...

• Macromolecule: Name: Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform; type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 35.836348 KDa
• Recombinant expression: Organism: Trichoplusia ni (cabbage looper)

Sequence

String:

SNMDEKVFTK ELDQWIEQLN ECKQLSESQV KSLCEKAKEI LTKESNVQEV RCPVTVCGDV HGQFHDLMEL FRIGGKSPDT NYLFMGDYV NRGYYSVETV TLLVALKVRY RERITILRGN HESRQITQVY GFYDECLRKY GNANVWKYFT DLFDYLPLTA L VDGQIFCL HGGLSPSIDT LDHIRALDRL QEVPHEGPMC DLLWSDPDDR GGWGISPRGA GYTFGQDISE TFNHANGLTL VS RAHQLVM EGYNWCHDRN VVTIFSAPNY CYRCGNQAAI MELDDTLKYS FLQFDPAPRR GEPHVTRRTP DYFL

UniProtKB: Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform

Macromolecule #20: DSS1

• Macromolecule: Name: DSS1 / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trichoplusia ni (cabbage looper)
• Molecular weight: Theoretical: 3.383542 KDa

Sequence

String:

DVSVWEENWE DDIVQDDFNQ QLRLEME

Macromolecule #21: Integrator complex subunit 13

• Macromolecule: Name: Integrator complex subunit 13 / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 80.34518 KDa
• Recombinant expression: Organism: Trichoplusia ni (cabbage looper)

Sequence

String:

MKIFSESHKT VFVVDHCPYM AESCRQHVEF DMLVKNRTQG IIPLAPISKS LWTCSVESSM EYCRIMYDIF PFKKLVNFIV SDSGAHVLN SWTQEDQNLQ ELMAALAAVG PPNPRADPEC CSILHGLVAA VETLCKITEY QHEARTLLME NAERVGNRGR I ICITNAKS DSHVRMLEDC VQETIHEHNK LAANSDHLMQ IQKCELVLIH TYPVGEDSLV SDRSKKELSP VLTSEVHSVR AG RHLATKL NILVQQHFDL ASTTITNIPM KEEQHANTSA NYDVELLHHK DAHVDFLKSG DSHLGGGSRE GSFKETITLK WCT PRTNNI ELHYCTGAYR ISPVDVNSRP SSCLTNFLLN GRSVLLEQPR KSGSKVISHM LSSHGGEIFL HVLSSSRSIL EDPP SISEG CGGRVTDYRI TDFGEFMREN RLTPFLDPRY KIDGSLEVPL ERAKDQLEKH TRYWPMIISQ TTIFNMQAVV PLASV IVKE SLTEEDVLNC QKTIYNLVDM ERKNDPLPIS TVGTRGKGPK RDEQYRIMWN ELETLVRAHI NNSEKHQRVL ECLMAC RSK PPEEEERKKR GRKREDKEDK SEKAVKDYEQ EKSWQDSERL KGILERGKEE LAEAEIIKDS PDSPEPPNKK PLVEMDE TP QVEKSKGPVS LLSLWSNRIN TANSRKHQEF AGRLNSVNNR AELYQHLKEE NGMETTENGK ASRQ

UniProtKB: Integrator complex subunit 13

Macromolecule #22: ZINC ION

• Macromolecule: Name: ZINC ION / type: ligand / ID: 22 / Number of copies: 2 / Formula: ZN
• Molecular weight: Theoretical: 65.409 Da

Macromolecule #23: MANGANESE (II) ION

• Macromolecule: Name: MANGANESE (II) ION / type: ligand / ID: 23 / Number of copies: 2 / Formula: MN
• Molecular weight: Theoretical: 54.938 Da

Experimental details

Structure determination

• Method: cryo EM
• Processing: single particle reconstruction
• Aggregation state: particle

Sample preparation

• Concentration: 0.3 mg/mL
• Buffer: pH: 7.4
• Vitrification: Cryogen name: ETHANE

Electron microscopy

• Microscope: FEI TITAN KRIOS
• Image recording: Film or detector model: GATAN K3 (6k x 4k) / Average electron dose: 39.93 e/Ų
• Electron beam: Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
• Electron optics: Illumination mode: OTHER / Imaging mode: OTHER / Nominal defocus max: 2.0 µm / Nominal defocus min: 0.5 µm
• Experimental equipment: Model: Titan Krios / Image courtesy: FEI Company

Image processing

• Startup model: Type of model: INSILICO MODEL
• Final reconstruction: Resolution.type: BY AUTHOR / Resolution: 3.7 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 236382
• Initial angle assignment: Type: MAXIMUM LIKELIHOOD
• Final angle assignment: Type: NOT APPLICABLE

Atomic model buiding 1

Initial model

PDB ID:

4oww

Chain - Source name: PDB / Chain - Initial model type: experimental model

Output model

PDB-8rbz:
Structure of Integrator-PP2A-SOSS-CTD post-termination complex

Atomic model buiding 2

Initial model

PDB ID:

8rbx

Chain - Source name: PDB / Chain - Initial model type: experimental model

Output model

PDB-8rbz:
Structure of Integrator-PP2A-SOSS-CTD post-termination complex