Film or detector model: FEI FALCON IV (4k x 4k) / Digitization - Dimensions - Width: 4096 pixel / Digitization - Dimensions - Height: 4096 pixel / Number grids imaged: 1 / Number real images: 7983 / Average electron dose: 50.0 e/Å2
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company
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Image processing
Particle selection
Number selected: 2303719
Startup model
Type of model: INSILICO MODEL In silico model: a startup model was generated using AlphaFold predicted monomers that were superimposed onto 20S PDB:7ZYJ Details: 7ZYJ LEISHMANIA TARENTOLAE PROTEASOME 20S SUBUNIT IN COMPLEX WITH COMPOUND was used as a template for the superimposition of all 14 AlphaFold-generated models.
Initial angle assignment
Type: RANDOM ASSIGNMENT
Final angle assignment
Type: OTHER
Final reconstruction
Applied symmetry - Point group: C2 (2 fold cyclic) / Resolution.type: BY AUTHOR / Resolution: 2.89 Å / Resolution method: FSC 0.143 CUT-OFF / Details: Standard Homo Refine job in Cryosparc v4 / Number images used: 14257
FSC plot (resolution estimation)
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