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- EMDB-16112: Human Urea Transporter UT-B/UT1 in Complex with Inhibitor UTBinh-14 -
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Open data
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Basic information
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Title | Human Urea Transporter UT-B/UT1 in Complex with Inhibitor UTBinh-14 | |||||||||
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![]() | SLC14A1 / UT1 / UT-B / Urea Transporter / Inhibitor / Solute Carrier / TRANSPORT PROTEIN | |||||||||
Function / homology | ![]() urea channel activity / urea transport / Transport of bile salts and organic acids, metal ions and amine compounds / urea transmembrane transport / urea transmembrane transporter activity / water transmembrane transporter activity / establishment of localization in cell / transmembrane transport / basolateral plasma membrane / plasma membrane Similarity search - Function | |||||||||
Biological species | ![]() | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 2.6 Å | |||||||||
![]() | Chi G / Dietz L / Pike ACW / Maclean EM / Mukhopadhyay SMM / Bohstedt T / Wang D / Scacioc A / McKinley G / Arrowsmith CH ...Chi G / Dietz L / Pike ACW / Maclean EM / Mukhopadhyay SMM / Bohstedt T / Wang D / Scacioc A / McKinley G / Arrowsmith CH / Edwards A / Bountra C / Fernandez-Cid A / Burgess-Brown NA / Duerr KL | |||||||||
Funding support | ![]()
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![]() | ![]() Title: Structural characterization of human urea transporters UT-A and UT-B and their inhibition. Authors: Gamma Chi / Larissa Dietz / Haiping Tang / Matthew Snee / Andreea Scacioc / Dong Wang / Gavin Mckinley / Shubhashish M M Mukhopadhyay / Ashley C W Pike / Rod Chalk / Nicola A Burgess-Brown / ...Authors: Gamma Chi / Larissa Dietz / Haiping Tang / Matthew Snee / Andreea Scacioc / Dong Wang / Gavin Mckinley / Shubhashish M M Mukhopadhyay / Ashley C W Pike / Rod Chalk / Nicola A Burgess-Brown / Jean-Pierre Timmermans / Wouter van Putte / Carol V Robinson / Katharina L Dürr / ![]() ![]() Abstract: In this study, we present the structures of human urea transporters UT-A and UT-B to characterize them at molecular level and to detail the mechanism of UT-B inhibition by its selective inhibitor, ...In this study, we present the structures of human urea transporters UT-A and UT-B to characterize them at molecular level and to detail the mechanism of UT-B inhibition by its selective inhibitor, UTB-14. High-resolution structures of both transporters establish the structural basis for the inhibitor's selectivity to UT-B, and the identification of multiple binding sites for the inhibitor will aid with the development of drug lead molecules targeting both transporters. Our study also discovers phospholipids associating with the urea transporters by combining structural observations, native MS, and lipidomics analysis. These insights improve our understanding of urea transporter function at a molecular level and provide a blueprint for a structure-guided design of therapeutics targeting these transporters. | |||||||||
History |
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Structure visualization
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 97 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 18.6 KB 18.6 KB | Display Display | ![]() |
FSC (resolution estimation) | ![]() | 15.3 KB | Display | ![]() |
Images | ![]() | 201.5 KB | ||
Masks | ![]() | 103 MB | ![]() | |
Filedesc metadata | ![]() | 6.2 KB | ||
Others | ![]() ![]() | 95.6 MB 95.6 MB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 8blpMC ![]() 8bloC C: citing same article ( M: atomic model generated by this map |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
EMDB pages | ![]() ![]() |
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Map
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Voxel size | X=Y=Z: 0.82 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Mask #1
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Density Histograms |
-Half map: #2
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Density Histograms |
-Half map: #1
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Density Histograms |
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Sample components
-Entire : Homotrimeric complex of Human Urea Transporter UT-B
Entire | Name: Homotrimeric complex of Human Urea Transporter UT-B |
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Components |
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-Supramolecule #1: Homotrimeric complex of Human Urea Transporter UT-B
Supramolecule | Name: Homotrimeric complex of Human Urea Transporter UT-B / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1 |
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Source (natural) | Organism: ![]() |
-Macromolecule #1: Urea transporter 1
Macromolecule | Name: Urea transporter 1 / type: protein_or_peptide / ID: 1 Details: N211A is an engineered mutation. The rest are natural variants. Number of copies: 3 / Enantiomer: LEVO |
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Source (natural) | Organism: ![]() |
Molecular weight | Theoretical: 40.17741 KDa |
Recombinant expression | Organism: ![]() |
Sequence | String: MFPKALGYVT GDMKELANQL KDKPVVLQFI DWILRGISQV VFVNNPVSGI LILVGLLVQN PWWALTGWLG TVVSTLMALL LSQDRSLIA SGLYGYNATL VGVLMAVFSD KGDYFWWLLL PVCAMSMTCP IFSSALNSVL SKWDLPVFTL PFNMALSMYL S ATGHYNPF ...String: MFPKALGYVT GDMKELANQL KDKPVVLQFI DWILRGISQV VFVNNPVSGI LILVGLLVQN PWWALTGWLG TVVSTLMALL LSQDRSLIA SGLYGYNATL VGVLMAVFSD KGDYFWWLLL PVCAMSMTCP IFSSALNSVL SKWDLPVFTL PFNMALSMYL S ATGHYNPF FPAKLVIPIT TAPQISWSDL SALELLKSIP VGVGQIYGCD NPWTGGIFLG AILLSSPLMC LHAAIGSLLG IA AGLSLSA PFENIYFGLW GFNSSLACIA MGGMFMALTW QTHLLALGCA LFTAYLGVGM ANFMAEVGLP ACTWPFCLAT LLF LIMTTK NSNIYKMPLS KVTYPEENRI FYLQAKKRMV ESPLAENLYF Q UniProtKB: Urea transporter 1 |
-Macromolecule #2: PHOSPHATIDYLETHANOLAMINE
Macromolecule | Name: PHOSPHATIDYLETHANOLAMINE / type: ligand / ID: 2 / Number of copies: 6 / Formula: PTY |
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Molecular weight | Theoretical: 734.039 Da |
Chemical component information | ![]() ChemComp-PTY: |
-Macromolecule #3: DODECYL-BETA-D-MALTOSIDE
Macromolecule | Name: DODECYL-BETA-D-MALTOSIDE / type: ligand / ID: 3 / Number of copies: 51 / Formula: LMT |
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Molecular weight | Theoretical: 510.615 Da |
Chemical component information | ![]() ChemComp-LMT: |
-Macromolecule #4: 10-(4-ethylphenyl)sulfonyl-~{N}-(thiophen-2-ylmethyl)-5-thia-1,8,...
Macromolecule | Name: 10-(4-ethylphenyl)sulfonyl-~{N}-(thiophen-2-ylmethyl)-5-thia-1,8,11,12-tetrazatricyclo[7.3.0.0^{2,6}]dodeca-2(6),3,7,9,11-pentaen-7-amine type: ligand / ID: 4 / Number of copies: 6 / Formula: 5D3 |
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Molecular weight | Theoretical: 455.576 Da |
Chemical component information | ![]() ChemComp-5D3: |
-Macromolecule #5: CHOLESTEROL HEMISUCCINATE
Macromolecule | Name: CHOLESTEROL HEMISUCCINATE / type: ligand / ID: 5 / Number of copies: 3 / Formula: Y01 |
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Molecular weight | Theoretical: 486.726 Da |
Chemical component information | ![]() ChemComp-Y01: |
-Macromolecule #6: water
Macromolecule | Name: water / type: ligand / ID: 6 / Number of copies: 66 / Formula: HOH |
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Molecular weight | Theoretical: 18.015 Da |
Chemical component information | ![]() ChemComp-HOH: |
-Experimental details
-Structure determination
Method | cryo EM |
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![]() | single particle reconstruction |
Aggregation state | particle |
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Sample preparation
Concentration | 3 mg/mL | |||||||||||||||
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Buffer | pH: 7.5 Component:
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Vitrification | Cryogen name: ETHANE |
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Electron microscopy
Microscope | TFS KRIOS |
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Image recording | Film or detector model: GATAN K2 SUMMIT (4k x 4k) / Number real images: 8221 / Average electron dose: 39.58 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.6 µm / Nominal defocus min: 0.8 µm |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |
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Image processing
-Atomic model buiding 1
Refinement | Protocol: AB INITIO MODEL |
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Output model | ![]() PDB-8blp: |