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- EMDB-15633: Negative stain EM reconstruction of the augmin holocomplex in clo... -

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Basic information

Entry
Database: EMDB / ID: EMD-15633
TitleNegative stain EM reconstruction of the augmin holocomplex in closed conformation
Map dataNegative stain EM reconstruction of the augmin holocomplex in closed conformation with calibrated pixel size
Sample
  • Complex: Augmin octameric holocomplex
    • Protein or peptide: HAUS augmin-like complex subunit 1
    • Protein or peptide: HAUS augmin-like complex subunit 3
    • Protein or peptide: HAUS augmin like complex subunit 4 L homeolog
    • Protein or peptide: HAUS augmin-like complex subunit 5
    • Protein or peptide: HAUS augmin like complex subunit 2 L homeolog
    • Protein or peptide: HAUS augmin like complex subunit 6 L homeolog
    • Protein or peptide: HAUS augmin like complex subunit 7 S homeolog
    • Protein or peptide: HAUS augmin-like complex subunit 8
KeywordsMicrotubule / Branching / Nucleation / CELL CYCLE
Function / homology
Function and homology information


HAUS complex / mitotic spindle microtubule / centrosome cycle / spindle assembly / spindle pole / spindle / microtubule / cell division / centrosome / cytoplasm
Similarity search - Function
HAUS augmin-like complex subunit 2, metazoa / HAUS augmin-like complex subunit 6 / HAUS augmin-like complex subunit 6, N-terminal / HAUS augmin-like complex subunit 2 / HAUS augmin-like complex subunit 7-like / HAUS augmin-like complex subunit 6 N-terminus / HAUS augmin-like complex subunit 2 / HAUS augmin-like complex subunit 4, metazoa / HAUS complex subunit 5, metazoa / HAUS augmin-like complex subunit 1 ...HAUS augmin-like complex subunit 2, metazoa / HAUS augmin-like complex subunit 6 / HAUS augmin-like complex subunit 6, N-terminal / HAUS augmin-like complex subunit 2 / HAUS augmin-like complex subunit 7-like / HAUS augmin-like complex subunit 6 N-terminus / HAUS augmin-like complex subunit 2 / HAUS augmin-like complex subunit 4, metazoa / HAUS complex subunit 5, metazoa / HAUS augmin-like complex subunit 1 / HAUS augmin-like complex subunit 5 / HAUS augmin-like complex subunit 4 / HAUS augmin-like complex subunit 4 / HAUS augmin-like complex subunit 5 / HAUS augmin-like complex subunit 3 / HAUS augmin-like complex subunit 3, N-terminal / HAUS augmin-like complex subunit 3
Similarity search - Domain/homology
HAUS augmin-like complex subunit 5 / HAUS augmin like complex subunit 6 L homeolog / HAUS augmin like complex subunit 7 S homeolog / HAUS augmin-like complex subunit 8 / LOC495502 protein / HAUS augmin like complex subunit 4 L homeolog / HAUS augmin-like complex subunit 3 / HAUS augmin like complex subunit 2 L homeolog
Similarity search - Component
Biological speciesXenopus laevis (African clawed frog)
Methodsingle particle reconstruction / negative staining / Resolution: 33.0 Å
AuthorsWuertz M / Pfeffer S
Funding support Germany, 2 items
OrganizationGrant numberCountry
German Research Foundation (DFG)PF 963/1-4 Germany
German Research Foundation (DFG)Schi 295/4-4 Germany
CitationJournal: Nat Commun / Year: 2022
Title: The augmin complex architecture reveals structural insights into microtubule branching.
Authors: Erik Zupa / Martin Würtz / Annett Neuner / Thomas Hoffmann / Mandy Rettel / Anna Böhler / Bram J A Vermeulen / Sebastian Eustermann / Elmar Schiebel / Stefan Pfeffer /
Abstract: In mitosis, the augmin complex binds to spindle microtubules to recruit the γ-tubulin ring complex (γ-TuRC), the principal microtubule nucleator, for the formation of branched microtubules. Our ...In mitosis, the augmin complex binds to spindle microtubules to recruit the γ-tubulin ring complex (γ-TuRC), the principal microtubule nucleator, for the formation of branched microtubules. Our understanding of augmin-mediated microtubule branching is hampered by the lack of structural information on the augmin complex. Here, we elucidate the molecular architecture and conformational plasticity of the augmin complex using an integrative structural biology approach. The elongated structure of the augmin complex is characterised by extensive coiled-coil segments and comprises two structural elements with distinct but complementary functions in γ-TuRC and microtubule binding, linked by a flexible hinge. The augmin complex is recruited to microtubules via a composite microtubule binding site comprising a positively charged unordered extension and two calponin homology domains. Our study provides the structural basis for augmin function in branched microtubule formation, decisively fostering our understanding of spindle formation in mitosis.
History
DepositionAug 22, 2022-
Header (metadata) releaseSep 28, 2022-
Map releaseSep 28, 2022-
UpdateDec 13, 2023-
Current statusDec 13, 2023Processing site: PDBe / Status: Released

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Structure visualization

Supplemental images

emd_15633.png

Downloads & links

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Map

FileDownload / File: emd_15633.map.gz / Format: CCP4 / Size: 64 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
AnnotationNegative stain EM reconstruction of the augmin holocomplex in closed conformation with calibrated pixel size
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
2.35 Å/pix.
x 256 pix.
= 601.6 Å		2.35 Å/pix.
x 256 pix.
= 601.6 Å		2.35 Å/pix.
x 256 pix.
= 601.6 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 2.35 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.0245
Minimum - Maximum-0.025224097 - 0.13776912
Average (Standard dev.)0.000109677196 (±0.0035088675)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions256256256
Spacing256256256
CellA=B=C: 601.6 Å
α=β=γ: 90.0 °

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Supplemental data

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Half map: Negative stain EM reconstruction of the augmin holocomplex...

Fileemd_15633_half_map_1.map
AnnotationNegative stain EM reconstruction of the augmin holocomplex in closed conformation half map 1
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: Negative stain EM reconstruction of the augmin holocomplex...

Fileemd_15633_half_map_2.map
AnnotationNegative stain EM reconstruction of the augmin holocomplex in closed conformation half map 2
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : Augmin octameric holocomplex

EntireName: Augmin octameric holocomplex
Components
  • Complex: Augmin octameric holocomplex
    • Protein or peptide: HAUS augmin-like complex subunit 1
    • Protein or peptide: HAUS augmin-like complex subunit 3
    • Protein or peptide: HAUS augmin like complex subunit 4 L homeolog
    • Protein or peptide: HAUS augmin-like complex subunit 5
    • Protein or peptide: HAUS augmin like complex subunit 2 L homeolog
    • Protein or peptide: HAUS augmin like complex subunit 6 L homeolog
    • Protein or peptide: HAUS augmin like complex subunit 7 S homeolog
    • Protein or peptide: HAUS augmin-like complex subunit 8

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Supramolecule #1: Augmin octameric holocomplex

SupramoleculeName: Augmin octameric holocomplex / type: complex / ID: 1 / Parent: 0 / Macromolecule list: all
Source (natural)Organism: Xenopus laevis (African clawed frog)
Molecular weightTheoretical: 435 KDa

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Macromolecule #1: HAUS augmin-like complex subunit 1

MacromoleculeName: HAUS augmin-like complex subunit 1 / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Xenopus laevis (African clawed frog)
Molecular weightTheoretical: 32.684684 KDa
Recombinant expressionOrganism: Spodoptera aff. frugiperda 1 BOLD-2017 (butterflies/moths)
SequenceString: MDEKSTKIIM WLKKMFGDKP LPPYEVNTRT MEILYQLAEW NEARDKDLSL VTEDLKLKSA EVKAEAKYLQ DLLTEGLGPS YTNLSRMGN NYLNQIVDSC LALELKNSSL SSYIPAVNDL SSELVAIELN NQEMEAELTS LRKKLTEALV LEKSLERDLK K AEEQCNFE ...String:
MDEKSTKIIM WLKKMFGDKP LPPYEVNTRT MEILYQLAEW NEARDKDLSL VTEDLKLKSA EVKAEAKYLQ DLLTEGLGPS YTNLSRMGN NYLNQIVDSC LALELKNSSL SSYIPAVNDL SSELVAIELN NQEMEAELTS LRKKLTEALV LEKSLERDLK K AEEQCNFE KAKVEIRSQN MKKLKDKSEE YKYKIHAAKD QLSSAGMEEP LTHRSLVSLS ETLTELKAQS MAAKEKLNSY LD LAPNPSL VKVKIEEAKR ELKATEVELT TKVNMMEFVV PEPSKRRLK

UniProtKB: LOC495502 protein

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Macromolecule #2: HAUS augmin-like complex subunit 3

MacromoleculeName: HAUS augmin-like complex subunit 3 / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Xenopus laevis (African clawed frog)
Molecular weightTheoretical: 68.11225 KDa
Recombinant expressionOrganism: Spodoptera aff. frugiperda 1 BOLD-2017 (butterflies/moths)
SequenceString: MSGGDRFVQT LQKLNYPKGA QLDGEDFDWL FEAVDLKPFL DWFCSAASEQ NVVPDEKLQA FNTLKESGKP VLDEKALDEV LKTFSISKV PAIEEVAIEK LEEEVKALQK QKNLHIRRRN KLQMVESGNR QMCLKSKDKE EETGRAFQEV LHLLRVTNKK L NHELQSIV ...String:
MSGGDRFVQT LQKLNYPKGA QLDGEDFDWL FEAVDLKPFL DWFCSAASEQ NVVPDEKLQA FNTLKESGKP VLDEKALDEV LKTFSISKV PAIEEVAIEK LEEEVKALQK QKNLHIRRRN KLQMVESGNR QMCLKSKDKE EETGRAFQEV LHLLRVTNKK L NHELQSIV NGVQTLMSFF STPETACELS SQPIFLSQLL LDKYLSLEEQ STAALTSFTK EHFFEGMSKF VEGSDENFQL VQ LNVNSFG EDGTTEDKCK EMMRLQLAYI CAKHKLIQMK AKSASLKVGL QWAENNASVV QDKASQKEEN LKVRITSLKN ETL QIENHT NSISNEKLPG LVRDNAQLLN MPIVKGDYDL QMAHQTSCSS RQDLVCDHLM KQKASFELLQ LGYELELRKH RDVY RELGS IVQELKESGD KLEERLTMLS DVNLLSASKP RSNIDSKDLT SHRLYQLLDG DNTQKLFRTY DGLESVAQKL SQDIA SMRD QLEVSEQEHS LLLSKLDSHL KELRDFMYPE GNTLMLTTPE LSGEFHQLGS QLEKLNHITV EILGDLQLKR KMLESN KLQ QIEKQLYVYF FQNEEQLKSI VGKLEAQTGG GSSA

UniProtKB: HAUS augmin-like complex subunit 3

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Macromolecule #3: HAUS augmin like complex subunit 4 L homeolog

MacromoleculeName: HAUS augmin like complex subunit 4 L homeolog / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Xenopus laevis (African clawed frog)
Molecular weightTheoretical: 41.256438 KDa
Recombinant expressionOrganism: Spodoptera aff. frugiperda 1 BOLD-2017 (butterflies/moths)
SequenceString: MAQTLQYVSS RLSMLQIDEE DLERNAQFGK VLIELCPLLG PNGGSANLNR ELEETRRELL LQRKMWMRSE VIYQLVQEML LDLQVRKLE GSLTEEERKF QDGLQQCMLV SECSRLLTAD SVPPSDSTSI LGLDKQDLLD LLPPNMLVLW VRDRLQKQLE E ALKKKCFT ...String:
MAQTLQYVSS RLSMLQIDEE DLERNAQFGK VLIELCPLLG PNGGSANLNR ELEETRRELL LQRKMWMRSE VIYQLVQEML LDLQVRKLE GSLTEEERKF QDGLQQCMLV SECSRLLTAD SVPPSDSTSI LGLDKQDLLD LLPPNMLVLW VRDRLQKQLE E ALKKKCFT FLSFHQPETD EEGDVLRAAK VLRLASTLED EKRRLQNEQE KHQEMRALLE KQQEIYPHVL LRCLSLLRQA AS ELRLRAQ SDIDRINAEY LEAKSNALFL KLRMEELQVL TDCYTPEKVL VHRQIRDTLE AGVKKEKQEL STSRQILSSY EFL GPEFEG LVQEYTRLKD KIKDNRWMLQ ELSKSLP

UniProtKB: HAUS augmin like complex subunit 4 L homeolog

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Macromolecule #4: HAUS augmin-like complex subunit 5

MacromoleculeName: HAUS augmin-like complex subunit 5 / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Xenopus laevis (African clawed frog)
Molecular weightTheoretical: 77.357281 KDa
Recombinant expressionOrganism: Spodoptera aff. frugiperda 1 BOLD-2017 (butterflies/moths)
SequenceString: MERRSLAQEL KKWAVEEMGL PAQKAPSEEM LQRLFIGQCG DIWKFIIRHI HSHRTVRKIE GNLLWYQQLQ HTEAQRTAEE EQQQRRKQL CKEILELRAE LHHLQEQIQT AEREIVGQDL NCERAQDLCR RSLLLRAFNK KREEECEALC QSNKKIQYRC E QLQEIRRA ...String:
MERRSLAQEL KKWAVEEMGL PAQKAPSEEM LQRLFIGQCG DIWKFIIRHI HSHRTVRKIE GNLLWYQQLQ HTEAQRTAEE EQQQRRKQL CKEILELRAE LHHLQEQIQT AEREIVGQDL NCERAQDLCR RSLLLRAFNK KREEECEALC QSNKKIQYRC E QLQEIRRA SQREVMFSAV DPDLSSSTFL EPEVLRDVRE VCKLRFKFLR SLHDDSISSS VHPGKEDLRS LSHQQWMSMA EK VWNTHTP NHILAALERL TLNSTQELKK LQFSQAADLS KGPSCQLKEF SEPITQSRSC NESTHLDPQE TLPSFHSLIQ EGW ANSVKV SSELRRVQSQ AQALSEHLAE RIQEIHKKLS DGSEVSVLTR AAFDAELRCV ILRGCRDALM QECRMLQEEA AGKK QEMKL LQQQQQNIQE ACLLLDKKQK HIQILIKGNS SSKSQIRRSS VEAQKYVQDK LLPWPQEIIQ ESQRLQDSIQ KEVKH FSAI CLPALLKVST DGFNLLPSRE LSINRMSNTH APYYGIFKGI YESVRLPLYK APESVLSHVA DMKKQLFFLR SQLSSR SEA ISKTQRALQK NTNPDTDALL KSLSDHYSLE LDEMVPKMQR LIQQCEKHQE YGKEVQATVM DWWEQPVQLC LPSEERG GL TLRQWRERWT VAVTALQRAT GSRS

UniProtKB: HAUS augmin-like complex subunit 5

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Macromolecule #5: HAUS augmin like complex subunit 2 L homeolog

MacromoleculeName: HAUS augmin like complex subunit 2 L homeolog / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Xenopus laevis (African clawed frog)
Molecular weightTheoretical: 25.135715 KDa
Recombinant expressionOrganism: Spodoptera aff. frugiperda 1 BOLD-2017 (butterflies/moths)
SequenceString: MMAANPWSPV QPSAASLLLE KCLAAGVLSQ NALDQAQLEA PCFSHAEELQ RITEIKAEIN QKSLELELLR LEKETADITH PLYLGQKHQ ALQAMNSHLE AVLREKRSLR QRLAQPVCQE NLPIEASYHR FTAELLPLAV NFIEKLEIYI KTIQTIPKIP D CASNMDNA ...String:
MMAANPWSPV QPSAASLLLE KCLAAGVLSQ NALDQAQLEA PCFSHAEELQ RITEIKAEIN QKSLELELLR LEKETADITH PLYLGQKHQ ALQAMNSHLE AVLREKRSLR QRLAQPVCQE NLPIEASYHR FTAELLPLAV NFIEKLEIYI KTIQTIPKIP D CASNMDNA LMRMESLEAE MEEVTEQILT WREQQKTAFQ MNSDANSSCI TAQTSYLSIE NLHP

UniProtKB: HAUS augmin like complex subunit 2 L homeolog

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Macromolecule #6: HAUS augmin like complex subunit 6 L homeolog

MacromoleculeName: HAUS augmin like complex subunit 6 L homeolog / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Xenopus laevis (African clawed frog)
Molecular weightTheoretical: 110.863484 KDa
Recombinant expressionOrganism: Spodoptera aff. frugiperda 1 BOLD-2017 (butterflies/moths)
SequenceString: MQSGSRPHLA WQREHMWLAL QGLGFESGAE AANAGKTLVH VTFGVNMFDK PNKDAFYVVF HFLFGKLDNV RCKEVFRYCW PPLDKKRDA EFRKACCEWL KKISDEVGAG FPQVVASIFL SPGGPKFVHL LYHFARYVML QHIKRDADAG NVFISEALQS K IQDPQKAL ...String:
MQSGSRPHLA WQREHMWLAL QGLGFESGAE AANAGKTLVH VTFGVNMFDK PNKDAFYVVF HFLFGKLDNV RCKEVFRYCW PPLDKKRDA EFRKACCEWL KKISDEVGAG FPQVVASIFL SPGGPKFVHL LYHFARYVML QHIKRDADAG NVFISEALQS K IQDPQKAL ARNKLARQKY LKVLQKENLV IEEYQRKAQL LIKQIRDMRS EHVALQNQQK LAEKVDRKIS DKDENIQKTR CM WNTIMQM LKEMEKEVDV VDAVVRGNID QYCLDGTNAT LNIPNLLISR IESEMHRLQM DNVYEAGKVN LITVVQLLNE ALK LVSGER SLYDCKGVRL DLQYLHGKAK FESEVLTRLR NMRHKIKRED LVSIEKIIAD REREWERKWE KILGKCPFSL LKGL NPALE LNPPMAPFSF DPASEEVLKS SVFCHYPASL QEYSHKEKPL KDFNQDHSGE LVQRLIGATV LTRSGRKSTS SLGMA TPNR RRMSLNEREF QTPTMNDRIG FQRTPSSAVQ KRRADVSWKT AANSPLPCTP TPYKQDPKNM ARQQLAQQVA DYIVSE SPR SSGGRGMELD DLLGVLSSDP FLSRKEIPRT PENLISDIRS SWRKAIQSEE SLVVASPVAA PCMDSTAELE SAHCSQI DL SMACFLSTSH ASDQNDCSGT RIPHNTGGNK ASSLHSDVVH HESIDMKSEI DSSQPEDLSL PIKENEPADK LINLLDDK K ESENADTLTN PVEFIFSNQP ISKLMDKTMF ISVSGQENLS AHTTLSWNSS NMITSDSSSD THEIIQFGIL HETLPENAG NVSLNSTLSL GGNEEPFEKS ELSDHDFTFD RKEHSSRSME GKMDINSIRS RYEALKRTLT SLTDEEYHGD PDTSNMRFTK HKSESSLIL GSDVYSPVEK VLSLDLEYLT TPSPKDRKLS LPQLISFSPE TIRSEKQEDL LDVFESQDFV NPNKTFDFTS S GLDLQKST DEGPEQLIKL

UniProtKB: HAUS augmin like complex subunit 6 L homeolog

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Macromolecule #7: HAUS augmin like complex subunit 7 S homeolog

MacromoleculeName: HAUS augmin like complex subunit 7 S homeolog / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Xenopus laevis (African clawed frog)
Molecular weightTheoretical: 39.379605 KDa
Recombinant expressionOrganism: Spodoptera aff. frugiperda 1 BOLD-2017 (butterflies/moths)
SequenceString: MTGGKELGAA VELYERLQML SCPCLEGVYL TDPQSIYELL CTPSSHRLDI LQWLCSRIYP PVQEQLSSLK ESQTDTKVKE IAKLCFDLM LCHFDDLDLI RGHASPFKQI SFIGQLLDVI QYPDTISSNV ILESLSHSTE KNVVTCIREN EELLKELFSS P HFQATLSP ...String:
MTGGKELGAA VELYERLQML SCPCLEGVYL TDPQSIYELL CTPSSHRLDI LQWLCSRIYP PVQEQLSSLK ESQTDTKVKE IAKLCFDLM LCHFDDLDLI RGHASPFKQI SFIGQLLDVI QYPDTISSNV ILESLSHSTE KNVVTCIREN EELLKELFSS P HFQATLSP ECNPWPADFK PLLNAEESLQ KRATQSSKGK DMSNSVEALL EISSSLKALK EECVDLCSSV TDGDKVIQSL RL ALTDFHQ LTIAFNQIYA NEFQEHCGHP APHMSPMGPF FQFVHQSLST CFKELESIAQ FTETSENIVD VVRERHQSKE KWA GSTIST LCEKMKELRQ SYEAFQQSSL QD

UniProtKB: HAUS augmin like complex subunit 7 S homeolog

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Macromolecule #8: HAUS augmin-like complex subunit 8

MacromoleculeName: HAUS augmin-like complex subunit 8 / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Xenopus laevis (African clawed frog)
Molecular weightTheoretical: 41.144852 KDa
Recombinant expressionOrganism: Spodoptera aff. frugiperda 1 BOLD-2017 (butterflies/moths)
SequenceString: MSEAGVAPIE DGSQNSSGGS SGDAALKKSK GGAKVVKSRY MQIGRSKVSK NSLANTTVCS GGKVPERGSG GTPTRRSLAP HKAKITAAV PLPALDGSIF TKEDLQSTLL DGHRIARPDL DLSVINDRTL QKITPRPVVT SEQKKPKRDT TPVNLVPEDM V EMIESQTL ...String:
MSEAGVAPIE DGSQNSSGGS SGDAALKKSK GGAKVVKSRY MQIGRSKVSK NSLANTTVCS GGKVPERGSG GTPTRRSLAP HKAKITAAV PLPALDGSIF TKEDLQSTLL DGHRIARPDL DLSVINDRTL QKITPRPVVT SEQKKPKRDT TPVNLVPEDM V EMIESQTL LLTYLTIKMQ KNLFRLEEKA ERNLLLVNDQ KDQLQETIHM MKRDLTLLQR EERLRDLIEK QDEVLTPVVT SK DPFKDNY TTFATALDST RHQLAIKNIH ITGNRHRYLE ELQKHLAITK SLLEEIMPSH ASENAESFDT IKDLENIVLK TDE ELARSF RQILDLSFKV NKEISLQSQK AVEETCESAL VRQWYFDGSL P

UniProtKB: HAUS augmin-like complex subunit 8

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Experimental details

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Structure determination

Methodnegative staining
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 7.5
StainingType: NEGATIVE / Material: Uranyl Acetate
GridModel: Quantifoil / Material: COPPER/PALLADIUM / Mesh: 400 / Support film - Material: CARBON / Support film - topology: CONTINUOUS / Support film - Film thickness: 1 / Pretreatment - Type: GLOW DISCHARGE

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Electron microscopy

MicroscopeTFS TALOS L120C
Image recordingFilm or detector model: FEI CETA (4k x 4k) / Number grids imaged: 1 / Number real images: 583 / Average electron dose: 101.8 e/Å2
Electron beamAcceleration voltage: 120 kV / Electron source: LAB6
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.0 µm / Nominal defocus min: 2.0 µm
Experimental equipment
Model: Talos L120C / Image courtesy: FEI Company

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Image processing

Particle selectionNumber selected: 80837
Startup modelType of model: NONE
Final reconstructionApplied symmetry - Point group: C1 (asymmetric) / Algorithm: BACK PROJECTION / Resolution.type: BY AUTHOR / Resolution: 33.0 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: RELION (ver. 3.1) / Number images used: 10658
Initial angle assignmentType: MAXIMUM LIKELIHOOD / Software - Name: RELION (ver. 3.1)
Final angle assignmentType: MAXIMUM LIKELIHOOD / Software - Name: RELION (ver. 3.1)
Final 3D classificationSoftware - Name: RELION (ver. 3.1)

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Atomic model buiding 1

RefinementSpace: REAL / Protocol: RIGID BODY FIT
Output model

PDB-8at4:
Structure of the augmin holocomplex in closed conformation

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