+Open data
-Basic information
Entry | Database: EMDB / ID: EMD-15499 | |||||||||
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Title | 365 A SynPspA rod after incubation with ATP | |||||||||
Map data | ||||||||||
Sample |
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Keywords | Nucleotide binding / Helical assembly / ESCRT-III fold / Membrane remodeling / LIPID BINDING PROTEIN | |||||||||
Function / homology | PspA/IM30 / PspA/IM30 family / Chloroplast membrane-associated 30 kD protein Function and homology information | |||||||||
Biological species | Synechocystis sp. PCC 6803 (bacteria) | |||||||||
Method | helical reconstruction / cryo EM / Resolution: 6.9 Å | |||||||||
Authors | Junglas B / Hudina E / Schoennenbeck P / Ritter I / Santiago-Schuebel B / Huesgen P / Sachse C | |||||||||
Funding support | European Union, 1 items
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Citation | Journal: To Be Published Title: Non-canonical ATPase activity drives PspA membrane constriction Authors: Junglas B / Hudina E / Schoennenbeck P / Ritter I / Santiago-Schuebel B / Huesgen P / Sachse C | |||||||||
History |
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-Structure visualization
Supplemental images |
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-Downloads & links
-EMDB archive
Map data | emd_15499.map.gz | 8.9 MB | EMDB map data format | |
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Header (meta data) | emd-15499-v30.xml emd-15499.xml | 15.6 KB 15.6 KB | Display Display | EMDB header |
FSC (resolution estimation) | emd_15499_fsc.xml | 13.3 KB | Display | FSC data file |
Images | emd_15499.png | 47.6 KB | ||
Masks | emd_15499_msk_1.map | 244.1 MB | Mask map | |
Filedesc metadata | emd-15499.cif.gz | 5.3 KB | ||
Others | emd_15499_additional_1.map.gz emd_15499_half_map_1.map.gz emd_15499_half_map_2.map.gz | 46.2 MB 225.7 MB 225.7 MB | ||
Archive directory | http://ftp.pdbj.org/pub/emdb/structures/EMD-15499 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-15499 | HTTPS FTP |
-Validation report
Summary document | emd_15499_validation.pdf.gz | 1018 KB | Display | EMDB validaton report |
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Full document | emd_15499_full_validation.pdf.gz | 1017.5 KB | Display | |
Data in XML | emd_15499_validation.xml.gz | 22.2 KB | Display | |
Data in CIF | emd_15499_validation.cif.gz | 29.1 KB | Display | |
Arichive directory | https://ftp.pdbj.org/pub/emdb/validation_reports/EMD-15499 ftp://ftp.pdbj.org/pub/emdb/validation_reports/EMD-15499 | HTTPS FTP |
-Related structure data
Related structure data | 8al0MC 8akqC 8akrC 8aksC 8aktC 8akuC 8akvC 8akwC 8akxC 8akyC 8akzC M: atomic model generated by this map C: citing same article (ref.) |
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Similar structure data | Similarity search - Function & homologyF&H Search |
-Links
EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
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-Map
File | Download / File: emd_15499.map.gz / Format: CCP4 / Size: 244.1 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||
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Voxel size | X=Y=Z: 1.362 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Mask #1
File | emd_15499_msk_1.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Additional map: Local filtered map
File | emd_15499_additional_1.map | ||||||||||||
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Annotation | Local filtered map | ||||||||||||
Projections & Slices |
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Density Histograms |
-Half map: #1
File | emd_15499_half_map_1.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Half map: #2
File | emd_15499_half_map_2.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Sample components
-Entire : Phage shock protein A (PspA)
Entire | Name: Phage shock protein A (PspA) |
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Components |
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-Supramolecule #1: Phage shock protein A (PspA)
Supramolecule | Name: Phage shock protein A (PspA) / type: complex / ID: 1 / Parent: 0 / Macromolecule list: all |
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Source (natural) | Organism: Synechocystis sp. PCC 6803 (bacteria) |
Molecular weight | Theoretical: 182.9 kDa/nm |
-Macromolecule #1: Chloroplast membrane-associated 30 kD protein
Macromolecule | Name: Chloroplast membrane-associated 30 kD protein / type: protein_or_peptide / ID: 1 / Number of copies: 60 / Enantiomer: LEVO |
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Source (natural) | Organism: Synechocystis sp. PCC 6803 (bacteria) / Strain: PCC 6803 / Kazusa |
Molecular weight | Theoretical: 28.097758 KDa |
Recombinant expression | Organism: Escherichia coli BL21(DE3) (bacteria) |
Sequence | String: MGSSHHHHHH SSSAALEVLF QGPMELFNRV GRVLKSQLTH WQQQQEAPED LLERLLGEME LELIELRRAL AQTIATFKST ERQRDAQQL IAQRWYEKAQ AALDRGNEQL AREALGQRQS YQSHTEALGK SLGEQRALVE QVRGQLQKLE RKYLELKSQK N LYLARLKS ...String: MGSSHHHHHH SSSAALEVLF QGPMELFNRV GRVLKSQLTH WQQQQEAPED LLERLLGEME LELIELRRAL AQTIATFKST ERQRDAQQL IAQRWYEKAQ AALDRGNEQL AREALGQRQS YQSHTEALGK SLGEQRALVE QVRGQLQKLE RKYLELKSQK N LYLARLKS AIAAQKIEEI AGNLDNASAS SLFERIETKI LELEAERELL NPPPSPLDKK FEQWEEQQAV EATLAAMKAR RS LPPPSS UniProtKB: Chloroplast membrane-associated 30 kD protein |
-Experimental details
-Structure determination
Method | cryo EM |
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Processing | helical reconstruction |
Aggregation state | helical array |
-Sample preparation
Buffer | pH: 8 |
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Vitrification | Cryogen name: ETHANE |
-Electron microscopy
Microscope | FEI TALOS ARCTICA |
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Image recording | Film or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Average electron dose: 58.0 e/Å2 |
Electron beam | Acceleration voltage: 200 kV / Electron source: FIELD EMISSION GUN |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 4.0 µm / Nominal defocus min: 1.0 µm |
Experimental equipment | Model: Talos Arctica / Image courtesy: FEI Company |