+Open data
-Basic information
Entry | Database: EMDB / ID: EMD-15354 | ||||||||||||
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Title | IHMof beta-cardiac myosin heads region | ||||||||||||
Map data | map of the IHMof beta-cardiac myosin heads region | ||||||||||||
Sample |
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Keywords | Cardiac Myosin / Myosin / Human / folded-back off state / head-head region / CONTRACTILE PROTEIN | ||||||||||||
Biological species | Homo sapiens (human) | ||||||||||||
Method | single particle reconstruction / cryo EM / Resolution: 3.2 Å | ||||||||||||
Authors | Grinzato A / Kandiah E / Robert-Paganin J | ||||||||||||
Funding support | United States, France, 3 items
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Citation | Journal: Nat Commun / Year: 2023 Title: Cryo-EM structure of the folded-back state of human β-cardiac myosin. Authors: Alessandro Grinzato / Daniel Auguin / Carlos Kikuti / Neha Nandwani / Dihia Moussaoui / Divya Pathak / Eaazhisai Kandiah / Kathleen M Ruppel / James A Spudich / Anne Houdusse / Julien Robert-Paganin / Abstract: To save energy and precisely regulate cardiac contractility, cardiac muscle myosin heads are sequestered in an 'off' state that can be converted to an 'on' state when exertion is increased. The 'off' ...To save energy and precisely regulate cardiac contractility, cardiac muscle myosin heads are sequestered in an 'off' state that can be converted to an 'on' state when exertion is increased. The 'off' state is equated with a folded-back structure known as the interacting-heads motif (IHM), which is a regulatory feature of all class-2 muscle and non-muscle myosins. We report here the human β-cardiac myosin IHM structure determined by cryo-electron microscopy to 3.6 Å resolution, providing details of all the interfaces stabilizing the 'off' state. The structure shows that these interfaces are hot spots of hypertrophic cardiomyopathy mutations that are thought to cause hypercontractility by destabilizing the 'off' state. Importantly, the cardiac and smooth muscle myosin IHM structures dramatically differ, providing structural evidence for the divergent physiological regulation of these muscle types. The cardiac IHM structure will facilitate development of clinically useful new molecules that modulate IHM stability. | ||||||||||||
History |
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-Structure visualization
Supplemental images |
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-Downloads & links
-EMDB archive
Map data | emd_15354.map.gz | 450.8 MB | EMDB map data format | |
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Header (meta data) | emd-15354-v30.xml emd-15354.xml | 14.2 KB 14.2 KB | Display Display | EMDB header |
FSC (resolution estimation) | emd_15354_fsc.xml | 17.3 KB | Display | FSC data file |
Images | emd_15354.png | 100.9 KB | ||
Masks | emd_15354_msk_1.map | 476.8 MB | Mask map | |
Others | emd_15354_half_map_1.map.gz emd_15354_half_map_2.map.gz | 442.3 MB 442.3 MB | ||
Archive directory | http://ftp.pdbj.org/pub/emdb/structures/EMD-15354 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-15354 | HTTPS FTP |
-Validation report
Summary document | emd_15354_validation.pdf.gz | 1 MB | Display | EMDB validaton report |
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Full document | emd_15354_full_validation.pdf.gz | 1 MB | Display | |
Data in XML | emd_15354_validation.xml.gz | 24.9 KB | Display | |
Data in CIF | emd_15354_validation.cif.gz | 32.5 KB | Display | |
Arichive directory | https://ftp.pdbj.org/pub/emdb/validation_reports/EMD-15354 ftp://ftp.pdbj.org/pub/emdb/validation_reports/EMD-15354 | HTTPS FTP |
-Related structure data
-Links
EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
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-Map
File | Download / File: emd_15354.map.gz / Format: CCP4 / Size: 476.8 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||||||||||||||||||
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Annotation | map of the IHMof beta-cardiac myosin heads region | ||||||||||||||||||||||||||||||||||||
Projections & slices | Image control
Images are generated by Spider. | ||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 0.84 Å | ||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Mask #1
File | emd_15354_msk_1.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Half map: half map of the IHMof beta-cardiac myosin heads region
File | emd_15354_half_map_1.map | ||||||||||||
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Annotation | half map of the IHMof beta-cardiac myosin heads region | ||||||||||||
Projections & Slices |
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Density Histograms |
-Half map: half map of the IHMof beta-cardiac myosin heads region
File | emd_15354_half_map_2.map | ||||||||||||
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Annotation | half map of the IHMof beta-cardiac myosin heads region | ||||||||||||
Projections & Slices |
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Density Histograms |
-Sample components
-Entire : human beta-cardiac myosin folded-back off state
Entire | Name: human beta-cardiac myosin folded-back off state |
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Components |
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-Supramolecule #1: human beta-cardiac myosin folded-back off state
Supramolecule | Name: human beta-cardiac myosin folded-back off state / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#5 |
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Source (natural) | Organism: Homo sapiens (human) |
-Experimental details
-Structure determination
Method | cryo EM |
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Processing | single particle reconstruction |
Aggregation state | particle |
-Sample preparation
Concentration | 0.3 mg/mL |
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Buffer | pH: 7.5 |
Vitrification | Cryogen name: ETHANE |
-Electron microscopy
Microscope | FEI TITAN KRIOS |
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Image recording | Film or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Average electron dose: 40.0 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 20.0 µm / Nominal defocus min: 8.0 µm |
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |