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Yorodumi- EMDB-14352: Structure of the GroEL chaperonin in complex with the CnoX chaper... -
+Open data
-Basic information
Entry | Database: EMDB / ID: EMD-14352 | |||||||||
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Title | Structure of the GroEL chaperonin in complex with the CnoX chaperedoxin | |||||||||
Map data | Map generated by phenix.resolve after relion refinement | |||||||||
Sample |
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Keywords | Protein Folding / Redox / Complex / Chaperonin / CHAPERONE | |||||||||
Function / homology | : / : Function and homology information | |||||||||
Biological species | Escherichia coli (E. coli) | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 3.4 Å | |||||||||
Authors | Van der Verren SE / Remaut H | |||||||||
Funding support | Belgium, 2 items
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Citation | Journal: To Be Published Title: A molecular plugin rescues GroEL/ES substrates from pre-folding oxidation Authors: Dupuy E / Van der Verren SE / Lin J / Wilson M / Viela F / Latour E / Dachsbeck A / Gennaris A / Vertommen D / Dufresne Y / Iorga B / Goemans CV / Remaut H / Collet JF | |||||||||
History |
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-Structure visualization
Supplemental images |
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-Downloads & links
-EMDB archive
Map data | emd_14352.map.gz | 7.2 MB | EMDB map data format | |
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Header (meta data) | emd-14352-v30.xml emd-14352.xml | 15.4 KB 15.4 KB | Display Display | EMDB header |
FSC (resolution estimation) | emd_14352_fsc.xml | 9.1 KB | Display | FSC data file |
Images | emd_14352.png | 75.6 KB | ||
Filedesc metadata | emd-14352.cif.gz | 5.8 KB | ||
Others | emd_14352_additional_1.map.gz | 9.7 MB | ||
Archive directory | http://ftp.pdbj.org/pub/emdb/structures/EMD-14352 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-14352 | HTTPS FTP |
-Validation report
Summary document | emd_14352_validation.pdf.gz | 480.9 KB | Display | EMDB validaton report |
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Full document | emd_14352_full_validation.pdf.gz | 480.5 KB | Display | |
Data in XML | emd_14352_validation.xml.gz | 9.8 KB | Display | |
Data in CIF | emd_14352_validation.cif.gz | 12.7 KB | Display | |
Arichive directory | https://ftp.pdbj.org/pub/emdb/validation_reports/EMD-14352 ftp://ftp.pdbj.org/pub/emdb/validation_reports/EMD-14352 | HTTPS FTP |
-Related structure data
Related structure data | 7ywyMC M: atomic model generated by this map C: citing same article (ref.) |
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Similar structure data | Similarity search - Function & homologyF&H Search |
-Links
EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
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-Map
File | Download / File: emd_14352.map.gz / Format: CCP4 / Size: 30.5 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||||||||||||||||||
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Annotation | Map generated by phenix.resolve after relion refinement | ||||||||||||||||||||||||||||||||||||
Projections & slices | Image control
Images are generated by Spider. | ||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 1.568 Å | ||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Additional map: Local sharpened map as generated by LocScale to help visualise CnoX
File | emd_14352_additional_1.map | ||||||||||||
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Annotation | Local sharpened map as generated by LocScale to help visualise CnoX | ||||||||||||
Projections & Slices |
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Density Histograms |
-Sample components
-Entire : 1:1 stoichiometric complex of GroEL-CnoX
Entire | Name: 1:1 stoichiometric complex of GroEL-CnoX |
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Components |
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-Supramolecule #1: 1:1 stoichiometric complex of GroEL-CnoX
Supramolecule | Name: 1:1 stoichiometric complex of GroEL-CnoX / type: complex / ID: 1 / Parent: 0 / Macromolecule list: all |
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Source (natural) | Organism: Escherichia coli (E. coli) |
-Macromolecule #1: Chaperedoxin
Macromolecule | Name: Chaperedoxin / type: protein_or_peptide / ID: 1 / Number of copies: 14 / Enantiomer: LEVO |
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Source (natural) | Organism: Escherichia coli (E. coli) |
Molecular weight | Theoretical: 9.771923 KDa |
Recombinant expression | Organism: Escherichia coli (E. coli) |
Sequence | String: ADTPEIQQLQ QQVAENPEDA ALATQLALQL HQVGRNEEAL ELLFGHLRKD LTAADGQTRK TFQEILAALG TGDALASKYR RQLYALLY UniProtKB: UNIPROTKB: A0A7U2VUH6 |
-Macromolecule #2: Chaperonin GroEL
Macromolecule | Name: Chaperonin GroEL / type: protein_or_peptide / ID: 2 / Number of copies: 14 / Enantiomer: LEVO |
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Source (natural) | Organism: Escherichia coli (E. coli) |
Molecular weight | Theoretical: 55.220105 KDa |
Recombinant expression | Organism: Escherichia coli (E. coli) |
Sequence | String: AAKDVKFGND ARVKMLRGVN VLADAVKVTL GPKGRNVVLD KSFGAPTITK DGVSVAREIE LEDKFENMGA QMVKEVASKA NDAAGDGTT TATVLAQAII TEGLKAVAAG MNPMDLKRGI DKAVTAAVEE LKALSVPCSD SKAIAQVGTI SANSDETVGK L IAEAMDKV ...String: AAKDVKFGND ARVKMLRGVN VLADAVKVTL GPKGRNVVLD KSFGAPTITK DGVSVAREIE LEDKFENMGA QMVKEVASKA NDAAGDGTT TATVLAQAII TEGLKAVAAG MNPMDLKRGI DKAVTAAVEE LKALSVPCSD SKAIAQVGTI SANSDETVGK L IAEAMDKV GKEGVITVED GTGLQDELDV VEGMQFDRGY LSPYFINKPE TGAVELESPF ILLADKKISN IREMLPVLEA VA KAGKPLL IIAEDVEGEA LATLVVNTMR GIVKVAAVKA PGFGDRRKAM LQDIATLTGG TVISEEIGME LEKATLEDLG QAK RVVINK DTTTIIDGVG EEAAIQGRVA QIRQQIEEAT SDYDREKLQE RVAKLAGGVA VIKVGAATEV EMKEKKARVE DALH ATRAA VEEGVVAGGG VALIRVASKL ADLRGQNEDQ NVGIKVALRA MEAPLRQIVL NCGEEPSVVA NTVKGGDGNY GYNAA TEEY GNMIDMGILD PTKVTRSALQ YAASVAGLMI TTECMVTDLP UniProtKB: UNIPROTKB: A0A828EVF1 |
-Experimental details
-Structure determination
Method | cryo EM |
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Processing | single particle reconstruction |
Aggregation state | particle |
-Sample preparation
Concentration | 0.4 mg/mL |
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Buffer | pH: 8 / Details: 50mM Tris pH=8, 150mM NaCl, 1mM EDTA |
Vitrification | Cryogen name: ETHANE / Instrument: GATAN CRYOPLUNGE 3 |
Details | Strep-affinity purified complex |
-Electron microscopy
Microscope | JEOL CRYO ARM 300 |
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Image recording | Film or detector model: GATAN K3 (6k x 4k) / Average electron dose: 68.3 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN |
Electron optics | Illumination mode: OTHER / Imaging mode: OTHER / Nominal defocus max: 3.0 µm / Nominal defocus min: 0.5 µm / Nominal magnification: 60000 |
+Image processing
-Atomic model buiding 1
Initial model |
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Refinement | Space: REAL / Protocol: FLEXIBLE FIT / Target criteria: Correlation coeficient | ||||||
Output model | PDB-7ywy: |