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- EMDB-13244: PoxtA-EQ2 antibiotic resistance ABCF bound to E. faecalis 70S rib... -

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Basic information

Entry
Database: EMDB / ID: EMD-13244
TitlePoxtA-EQ2 antibiotic resistance ABCF bound to E. faecalis 70S ribosome, state III
Map dataPoxtA bound to 70S ribosome from E. faecalis, state III. From RELION Refine3d.
Sample
  • Complex: Complex of PoxtA-EQ2 bound to the 70S ribosome from E. faecalis (initiation complex), state III.
    • Complex: ARE-ABC-F family resistance factor PoxtA
      • Protein or peptide: x 1 types
    • Complex: A-site tRNA
      • RNA: x 1 types
    • Organelle or cellular component: 70S Ribosome
      • Protein or peptide: x 47 types
      • RNA: x 5 types
  • Ligand: x 7 types
KeywordsRibosome / Enterococcus faecalis / PoxtA / ABCF / antibiotic resistance protein
Function / homology
Function and homology information


ribosomal small subunit biogenesis / small ribosomal subunit rRNA binding / large ribosomal subunit / regulation of translation / ribosomal small subunit assembly / large ribosomal subunit rRNA binding / small ribosomal subunit / 5S rRNA binding / transferase activity / cytosolic small ribosomal subunit ...ribosomal small subunit biogenesis / small ribosomal subunit rRNA binding / large ribosomal subunit / regulation of translation / ribosomal small subunit assembly / large ribosomal subunit rRNA binding / small ribosomal subunit / 5S rRNA binding / transferase activity / cytosolic small ribosomal subunit / ribosomal large subunit assembly / cytosolic large ribosomal subunit / tRNA binding / rRNA binding / ribosome / structural constituent of ribosome / translation / ribonucleoprotein complex / mRNA binding / zinc ion binding / cytosol / cytoplasm
Similarity search - Function
Ribosomal protein L31 type B / Type-1 KH domain profile. / Ribosomal protein L1, bacterial-type / : / Ribosomal protein S14, type Z / Ribosomal protein L1, conserved site / Ribosomal protein L1 signature. / Ribosomal protein L1 / Ribosomal protein L1, 3-layer alpha/beta-sandwich / Ribosomal protein L1-like ...Ribosomal protein L31 type B / Type-1 KH domain profile. / Ribosomal protein L1, bacterial-type / : / Ribosomal protein S14, type Z / Ribosomal protein L1, conserved site / Ribosomal protein L1 signature. / Ribosomal protein L1 / Ribosomal protein L1, 3-layer alpha/beta-sandwich / Ribosomal protein L1-like / Ribosomal protein L1/ribosomal biogenesis protein / Ribosomal protein L1p/L10e family / Ribosomal protein L31 signature. / Ribosomal protein L31 / Ribosomal protein L31 superfamily / Ribosomal protein L31 / Ribosomal protein L16 signature 1. / : / Ribosomal protein L6, conserved site / Ribosomal protein L6 signature 1. / Ribosomal protein L16, conserved site / Ribosomal protein L16 signature 2. / Ribosomal protein L17 signature. / Ribosomal protein L28/L24 superfamily / Ribosomal protein L36 signature. / Ribosomal protein L32p, bacterial type / : / : / Ribosomal protein L28 / Ribosomal protein L35, conserved site / Ribosomal protein L35 signature. / Ribosomal protein L33, conserved site / Ribosomal protein L33 signature. / Ribosomal protein L35, non-mitochondrial / Ribosomal protein L5, bacterial-type / Ribosomal protein L18, bacterial-type / Ribosomal protein L6, bacterial-type / Ribosomal protein S14/S29 / Ribosomal protein S3, bacterial-type / Ribosomal protein L19, conserved site / Ribosomal protein L19 signature. / Ribosomal protein S19, bacterial-type / Ribosomal protein S13, bacterial-type / Ribosomal protein L36 / Ribosomal protein L36 superfamily / Ribosomal protein L36 / Ribosomal protein S7, bacterial/organellar-type / Ribosomal protein S11, bacterial-type / Ribosomal protein S20 / Ribosomal protein S20 superfamily / Ribosomal protein S20 / Ribosomal protein L20 signature. / Ribosomal protein S9, bacterial/plastid / Ribosomal protein L27, conserved site / Ribosomal protein L27 signature. / Ribosomal protein S4, bacterial-type / 30S ribosomal protein S17 / Ribosomal protein S5, bacterial-type / Ribosomal protein L14P, bacterial-type / Ribosomal protein S6, plastid/chloroplast / Ribosomal protein L34, conserved site / Ribosomal protein L34 signature. / Ribosomal protein L22, bacterial/chloroplast-type / Ribosomal protein L2, bacterial/organellar-type / Ribosomal protein S2, bacteria/mitochondria/plastid / Ribosomal protein L35 / Ribosomal protein L35 superfamily / Ribosomal protein L35 / Ribosomal L28 family / Ribosomal protein L33 / Ribosomal protein L33 / Ribosomal protein L28/L24 / Ribosomal protein L18 / Ribosomal L18 of archaea, bacteria, mitoch. and chloroplast / Ribosomal protein L33 superfamily / Ribosomal protein L30, bacterial-type / Ribosomal protein S18, conserved site / Ribosomal protein S18 signature. / Ribosomal protein L16 / Ribosomal protein S16 / Ribosomal protein S16 domain superfamily / Ribosomal protein S16 / : / L28p-like / Ribosomal protein L20 / Ribosomal protein S15, bacterial-type / Ribosomal protein L20 / Ribosomal protein L20, C-terminal / Ribosomal protein L27 / Ribosomal L27 protein / Ribosomal protein L21 / Ribosomal protein S6 / Ribosomal protein S6 / Ribosomal protein L19 / Ribosomal protein L19 / Ribosomal protein S6 superfamily / Ribosomal protein L19 superfamily / Ribosomal proteins 50S L24/mitochondrial 39S L24 / Ribosomal protein L17 / Ribosomal protein L17 superfamily
Similarity search - Domain/homology
Small ribosomal subunit protein bS18 / Small ribosomal subunit protein bS6 / Small ribosomal subunit protein uS7 / Small ribosomal subunit protein uS12 / Large ribosomal subunit protein uL3 / Small ribosomal subunit protein uS10 / Large ribosomal subunit protein uL2 / Large ribosomal subunit protein uL23 / Small ribosomal subunit protein uS3 / Small ribosomal subunit protein uS19 ...Small ribosomal subunit protein bS18 / Small ribosomal subunit protein bS6 / Small ribosomal subunit protein uS7 / Small ribosomal subunit protein uS12 / Large ribosomal subunit protein uL3 / Small ribosomal subunit protein uS10 / Large ribosomal subunit protein uL2 / Large ribosomal subunit protein uL23 / Small ribosomal subunit protein uS3 / Small ribosomal subunit protein uS19 / Large ribosomal subunit protein uL14 / Large ribosomal subunit protein uL5 / Small ribosomal subunit protein uS17 / Small ribosomal subunit protein uS8 / Large ribosomal subunit protein uL18 / Small ribosomal subunit protein uS14 / Large ribosomal subunit protein uL15 / Large ribosomal subunit protein uL22 / Small ribosomal subunit protein uS13 / Large ribosomal subunit protein bL36 / Large ribosomal subunit protein uL16 / Large ribosomal subunit protein uL29 / Small ribosomal subunit protein uS11 / Large ribosomal subunit protein uL24 / Small ribosomal subunit protein uS5 / Large ribosomal subunit protein uL30 / Large ribosomal subunit protein bL17 / Large ribosomal subunit protein bL35 / Large ribosomal subunit protein bL20 / Large ribosomal subunit protein bL27 / Large ribosomal subunit protein bL21 / Large ribosomal subunit protein bL32 / Large ribosomal subunit protein bL31B / Small ribosomal subunit protein bS16 / Large ribosomal subunit protein bL19 / Small ribosomal subunit protein uS2 / Small ribosomal subunit protein bS20 / Large ribosomal subunit protein uL1 / Large ribosomal subunit protein bL33 / Small ribosomal subunit protein uS4 / Small ribosomal subunit protein uS15 / Large ribosomal subunit protein bL28 / Small ribosomal subunit protein uS9 / Large ribosomal subunit protein uL13 / Large ribosomal subunit protein bL34 / Large ribosomal subunit protein uL6 / Large ribosomal subunit protein uL4
Similarity search - Component
Biological speciesEnterococcus faecalis (bacteria) / Escherichia coli (E. coli)
Methodsingle particle reconstruction / cryo EM / Resolution: 2.9 Å
AuthorsCrowe-McAuliffe C / Wilson DN
Funding support Germany, Sweden, 2 items
OrganizationGrant numberCountry
German Research Foundation (DFG)WI3285/8-1 Germany
Swedish Research Council2017-03783 Sweden
Citation
Journal: mSphere / Year: 2018
Title: The Enterococcus Cassette Chromosome, a Genomic Variation Enabler in Enterococci.
Authors: A Sivertsen / J Janice / T Pedersen / T M Wagner / J Hegstad / K Hegstad /
Abstract: has a highly variable genome prone to recombination and horizontal gene transfer. Here, we have identified a novel genetic island with an insertion locus and mobilization genes similar to those of ... has a highly variable genome prone to recombination and horizontal gene transfer. Here, we have identified a novel genetic island with an insertion locus and mobilization genes similar to those of staphylococcus cassette chromosome elements SCC This novel element termed the enterococcus cassette chromosome (ECC) element was located in the 3' region of and encoded large serine recombinases similar to SCC Horizontal transfer of an ECC element termed ECC:: containing a knock-in chloramphenicol resistance determinant occurred in the presence of a conjugative plasmid. We determined the ECC:: insertion site in the 3' region of in the recipient by long-read sequencing. ECC:: also mobilized by homologous recombination through sequence identity between flanking insertion sequence (IS) elements in ECC:: and the conjugative plasmid. The genes were found in 69 of 516 genomes in GenBank. Full-length ECC elements were retrieved from 32 of these genomes. ECCs were flanked by and sites of approximately 50 bp. The sequences were found by PCR and sequencing of circularized ECCs in three strains. The genes in ECCs contained an amalgam of common and rare genes. Taken together, our data imply that ECC elements act as hot spots for genetic exchange and contribute to the large variation of accessory genes found in is a bacterium found in a great variety of environments, ranging from the clinic as a nosocomial pathogen to natural habitats such as mammalian intestines, water, and soil. They are known to exchange genetic material through horizontal gene transfer and recombination, leading to great variability of accessory genes and aiding environmental adaptation. Identifying mobile genetic elements causing sequence variation is important to understand how genetic content variation occurs. Here, a novel genetic island, the enterococcus cassette chromosome, is shown to contain a wealth of genes, which may aid in adapting to new environments. The transmission mechanism involves the only two conserved genes within ECC, , large serine recombinases that insert ECC into the host genome similarly to SCC elements found in staphylococci.
#1: Journal: Biorxiv / Year: 2021
Title: Structural basis for PoxtA-mediated resistance to Phenicol and Oxazolidinone antibiotics
Authors: Crowe-McAuliffe C / Murina V / Kasari M / Takada H / Turnbull KJ / Sundsfjord A / Hegstad K / Atkinson GC / Wilson DN / Hauryliuk V
History
DepositionJul 20, 2021-
Header (metadata) releaseFeb 23, 2022-
Map releaseFeb 23, 2022-
UpdateJul 17, 2024-
Current statusJul 17, 2024Processing site: PDBe / Status: Released

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 0.014
  • Imaged by UCSF Chimera
  • Download
  • Surface view colored by height
  • Surface level: 0.014
  • Imaged by UCSF Chimera
  • Download
  • Surface view with fitted model
  • Atomic models: PDB-7p7t
  • Surface level: 0.014
  • Imaged by UCSF Chimera
  • Download
Movie viewer
Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

emd_13244.png

Downloads & links

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Map

FileDownload / File: emd_13244.map.gz / Format: CCP4 / Size: 282.6 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
AnnotationPoxtA bound to 70S ribosome from E. faecalis, state III. From RELION Refine3d.
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
0.82 Å/pix.
x 420 pix.
= 344.4 Å		0.82 Å/pix.
x 420 pix.
= 344.4 Å		0.82 Å/pix.
x 420 pix.
= 344.4 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 0.82 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.014 / Movie #1: 0.014
Minimum - Maximum-0.010433473 - 0.05915874
Average (Standard dev.)0.0018360991 (±0.0042402456)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions420420420
Spacing420420420
CellA=B=C: 344.4 Å
α=β=γ: 90.0 °

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z0.820.820.82
M x/y/z420420420
origin x/y/z0.0000.0000.000
length x/y/z344.400344.400344.400
α/β/γ90.00090.00090.000
MAP C/R/S123
start NC/NR/NS000
NC/NR/NS420420420
D min/max/mean-0.0100.0590.002

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Supplemental data

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Mask #1

Fileemd_13244_msk_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Additional map: PoxtA bound to 70S ribosome from E. faecalis,...

Fileemd_13244_additional_1.map
AnnotationPoxtA bound to 70S ribosome from E. faecalis, state III. Sharpened map.
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: PoxtA bound to 70S ribosome from E. faecalis, state III. Half map.

Fileemd_13244_half_map_1.map
AnnotationPoxtA bound to 70S ribosome from E. faecalis, state III. Half map.
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: PoxtA bound to 70S ribosome from E. faecalis, state III. Half map.

Fileemd_13244_half_map_2.map
AnnotationPoxtA bound to 70S ribosome from E. faecalis, state III. Half map.
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : Complex of PoxtA-EQ2 bound to the 70S ribosome from E. faecalis (...

EntireName: Complex of PoxtA-EQ2 bound to the 70S ribosome from E. faecalis (initiation complex), state III.
Components
  • Complex: Complex of PoxtA-EQ2 bound to the 70S ribosome from E. faecalis (initiation complex), state III.
    • Complex: ARE-ABC-F family resistance factor PoxtA
      • Protein or peptide: ARE-ABC-F family resistance factor PoxtA
    • Complex: A-site tRNA
      • RNA: A-site tRNA
    • Organelle or cellular component: 70S Ribosome
      • Protein or peptide: 50S ribosomal protein L29
      • Protein or peptide: 50S ribosomal protein L30
      • Protein or peptide: 50S ribosomal protein L31 type B
      • Protein or peptide: 50S ribosomal protein L32
      • Protein or peptide: 50S ribosomal protein L33
      • Protein or peptide: 50S ribosomal protein L34
      • Protein or peptide: 50S ribosomal protein L35
      • Protein or peptide: 50S ribosomal protein L36
      • RNA: 23S rRNA
      • RNA: 5S rRNA
      • RNA: tRNA-fMet
      • Protein or peptide: 50S ribosomal protein L1
      • Protein or peptide: 50S ribosomal protein L2
      • Protein or peptide: 50S ribosomal protein L3
      • Protein or peptide: 50S ribosomal protein L4
      • Protein or peptide: 50S ribosomal protein L5
      • Protein or peptide: 50S ribosomal protein L6
      • Protein or peptide: 50S ribosomal protein L13
      • Protein or peptide: 50S ribosomal protein L14
      • Protein or peptide: 50S ribosomal protein L15
      • Protein or peptide: 50S ribosomal protein L16
      • Protein or peptide: 50S ribosomal protein L17
      • Protein or peptide: 50S ribosomal protein L18
      • Protein or peptide: 50S ribosomal protein L19
      • Protein or peptide: 50S ribosomal protein L20
      • Protein or peptide: 50S ribosomal protein L21
      • Protein or peptide: 50S ribosomal protein L22
      • Protein or peptide: 50S ribosomal protein L23
      • Protein or peptide: 50S ribosomal protein L24
      • Protein or peptide: 50S ribosomal protein L27
      • Protein or peptide: 50S ribosomal protein L28
      • RNA: 16S rRNA
      • RNA: mRNA
      • Protein or peptide: 30S ribosomal protein S2
      • Protein or peptide: 30S ribosomal protein S3
      • Protein or peptide: 30S ribosomal protein S4
      • Protein or peptide: 30S ribosomal protein S5
      • Protein or peptide: 30S ribosomal protein S6
      • Protein or peptide: 30S ribosomal protein S7
      • Protein or peptide: 30S ribosomal protein S8
      • Protein or peptide: 30S ribosomal protein S9
      • Protein or peptide: 30S ribosomal protein S10
      • Protein or peptide: 30S ribosomal protein S11
      • Protein or peptide: 30S ribosomal protein S12
      • Protein or peptide: 30S ribosomal protein S13
      • Protein or peptide: 30S ribosomal protein S14 type Z
      • Protein or peptide: 30S ribosomal protein S15
      • Protein or peptide: 30S ribosomal protein S16
      • Protein or peptide: 30S ribosomal protein S17
      • Protein or peptide: 30S ribosomal protein S18
      • Protein or peptide: 30S ribosomal protein S19
      • Protein or peptide: 30S ribosomal protein S20
  • Ligand: ADENOSINE-5'-TRIPHOSPHATE
  • Ligand: MAGNESIUM ION
  • Ligand: ZINC ION
  • Ligand: POTASSIUM ION
  • Ligand: SPECTINOMYCIN
  • Ligand: 1,4-DIAMINOBUTANE
  • Ligand: water

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Supramolecule #1: Complex of PoxtA-EQ2 bound to the 70S ribosome from E. faecalis (...

SupramoleculeName: Complex of PoxtA-EQ2 bound to the 70S ribosome from E. faecalis (initiation complex), state III.
type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#54
Details: Obtained by affinity purification of FLAG-tagged PoxtA.

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Supramolecule #2: ARE-ABC-F family resistance factor PoxtA

SupramoleculeName: ARE-ABC-F family resistance factor PoxtA / type: complex / ID: 2 / Parent: 1 / Macromolecule list: #1
Source (natural)Organism: Enterococcus faecalis (bacteria)

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Supramolecule #4: A-site tRNA

SupramoleculeName: A-site tRNA / type: complex / ID: 4 / Parent: 1 / Macromolecule list: #12
Source (natural)Organism: Escherichia coli (E. coli)

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Supramolecule #3: 70S Ribosome

SupramoleculeName: 70S Ribosome / type: organelle_or_cellular_component / ID: 3 / Parent: 1 / Macromolecule list: #2-#11, #13-#54
Source (natural)Organism: Enterococcus faecalis (bacteria)

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Macromolecule #1: ARE-ABC-F family resistance factor PoxtA

MacromoleculeName: ARE-ABC-F family resistance factor PoxtA / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Enterococcus faecalis (bacteria)
Molecular weightTheoretical: 67.890656 KDa
Recombinant expressionOrganism: Enterococcus faecalis (bacteria)
SequenceString: MRGQKMNLTF GLEEVYEDAE FHLGDFDKVG IVGVNGAGKT TLFRLLLREL KLDKGKITIG NSRIGYLPQE IIIMDEKMTV LEFLAEGRP IKKMETELNY IYEKLTVVDD DKQDRLLKRM GWLQEQLEYF DCYEAESILL DLVDSMQIDF DLFDQPISEL S GGQKSKIS ...String:
MRGQKMNLTF GLEEVYEDAE FHLGDFDKVG IVGVNGAGKT TLFRLLLREL KLDKGKITIG NSRIGYLPQE IIIMDEKMTV LEFLAEGRP IKKMETELNY IYEKLTVVDD DKQDRLLKRM GWLQEQLEYF DCYEAESILL DLVDSMQIDF DLFDQPISEL S GGQKSKIS FAKVLYSKPE ILLLDQPTNH LDSSTKEFVT KYLKSYRGSV LIISHDIDFL NQIIDKIMYI DKVTHKISIY EG DYYTYKK KYSENQLLRE KMIIQQEKEV KELATFVQKA KQASQTNHAL KRMGLERAIR LDKKKKNLTK RDKVYKRVKM DIK PNREVA RTPLKVENVT FHYPGHPILY KDLSFQISGK ERFLVVGENG VGKSTLLKLL MGINIPDKGK IIFNDKTDVA YYSQ ELEQL DEEKTILENV KSDEYTDWKL RATLSNFLFY DDDVNKKVNV LSPGEKARIV LCKVLLQKAN LLILDQPTNH LDPET QSII GANFNLFEGT IIVVSHNPSF VEQIGINRML ILPSGNIDNY SPELLEYYSN LNMIKEIQVG HHHHHHAKGG ENLYFQ GVA DYKDHDGDYK DHDIDYKDDD DKG

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Macromolecule #2: 50S ribosomal protein L29

MacromoleculeName: 50S ribosomal protein L29 / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Enterococcus faecalis (bacteria)
Molecular weightTheoretical: 7.342562 KDa
SequenceString:
MKVKEIRELT TAEMLDKEKQ LKEELFNLRF QLATGQLENT ARIKEVRQSI ARIKTVLREQ AN

UniProtKB: Large ribosomal subunit protein uL29

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Macromolecule #3: 50S ribosomal protein L30

MacromoleculeName: 50S ribosomal protein L30 / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Enterococcus faecalis (bacteria)
Molecular weightTheoretical: 6.365469 KDa
SequenceString:
MAELKITLKR SVIGRPQNQR ATVKALGLGK VNSTVTKPAN EAIKGMVNTI SHLVDVEEV

UniProtKB: Large ribosomal subunit protein uL30

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Macromolecule #4: 50S ribosomal protein L31 type B

MacromoleculeName: 50S ribosomal protein L31 type B / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Enterococcus faecalis (bacteria)
Molecular weightTheoretical: 10.138101 KDa
SequenceString:
MKQDIHPNYQ PVVFMDSTTG FKFLSGSTKG SSETVEWEDG NTYPLLRVEV TSDSHPFYTG RQKFTQADGR VDRFNKKYGL KDENANPDA

UniProtKB: Large ribosomal subunit protein bL31B

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Macromolecule #5: 50S ribosomal protein L32

MacromoleculeName: 50S ribosomal protein L32 / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Enterococcus faecalis (bacteria)
Molecular weightTheoretical: 6.548759 KDa
SequenceString:
MAVPARRTSK AKKAKRRTHY KLTIKGLNAC SNCGEMKKSH HVCPACGHYD GKDVMSKEA

UniProtKB: Large ribosomal subunit protein bL32

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Macromolecule #6: 50S ribosomal protein L33

MacromoleculeName: 50S ribosomal protein L33 / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Enterococcus faecalis (bacteria)
Molecular weightTheoretical: 6.052027 KDa
SequenceString:
MRVNITLECT SCKERNYLTN KNKRNNPDRL EKQKYCPRER KVTLHRETK

UniProtKB: Large ribosomal subunit protein bL33

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Macromolecule #7: 50S ribosomal protein L34

MacromoleculeName: 50S ribosomal protein L34 / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Enterococcus faecalis (bacteria)
Molecular weightTheoretical: 5.373507 KDa
SequenceString:
MKRTYQPNKR KRQKVHGFRK RMSTKNGRRV LASRRRKGRK VISA

UniProtKB: Large ribosomal subunit protein bL34

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Macromolecule #8: 50S ribosomal protein L35

MacromoleculeName: 50S ribosomal protein L35 / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Enterococcus faecalis (bacteria)
Molecular weightTheoretical: 7.727316 KDa
SequenceString:
MPKQKTHRGL AKRVKRTGGG GLKRGRAFTS HRFHGKTKKQ RRQLRKASMV AKGDYKRIRQ QLARMK

UniProtKB: Large ribosomal subunit protein bL35

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Macromolecule #9: 50S ribosomal protein L36

MacromoleculeName: 50S ribosomal protein L36 / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Enterococcus faecalis (bacteria)
Molecular weightTheoretical: 4.440534 KDa
SequenceString:
MKVRPSVKPM CEHCKVIRRK GRVMVICPAN PKHKQRQG

UniProtKB: Large ribosomal subunit protein bL36

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Macromolecule #14: 50S ribosomal protein L1

MacromoleculeName: 50S ribosomal protein L1 / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Enterococcus faecalis (bacteria)
Molecular weightTheoretical: 24.49523 KDa
SequenceString: MAKKSKKMQE ALKKVDATKA YSVEEAVALA KDTNIAKFDA TVEVAYKLNV DPKKADQQIR GAVVLPNGTG KTQTVLVFAK GEKAKEAEA AGADFVGDDD MVAKIQGGWF DFDVVVATPD MMATVGKLGR VLGPKGLMPN PKTGTVTMDV TKAVEEVKAG K VTYRVDKA ...String:
MAKKSKKMQE ALKKVDATKA YSVEEAVALA KDTNIAKFDA TVEVAYKLNV DPKKADQQIR GAVVLPNGTG KTQTVLVFAK GEKAKEAEA AGADFVGDDD MVAKIQGGWF DFDVVVATPD MMATVGKLGR VLGPKGLMPN PKTGTVTMDV TKAVEEVKAG K VTYRVDKA GNIHVPIGKV SFDNEKLVEN FNTINDVLLK AKPSTAKGQY IKNISVTTTF GPGIHVDQAS F

UniProtKB: Large ribosomal subunit protein uL1

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Macromolecule #15: 50S ribosomal protein L2

MacromoleculeName: 50S ribosomal protein L2 / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Enterococcus faecalis (bacteria)
Molecular weightTheoretical: 30.291008 KDa
SequenceString: MAIKKYKPTT NGRRNMTSSD FAEITTSTPE KSLLQPLKNN AGRNNNGRIT VRHQGGGHKR QYRVIDFKRN KDNVAAVVKT IEYDPNRSA NIALVHYEDG VKAYILAPKG LEVGMRLVSG PEADIKVGNA LPLENIPVGT VIHNIEMKPG KGGQLIRSAG T SAQVLGKE ...String:
MAIKKYKPTT NGRRNMTSSD FAEITTSTPE KSLLQPLKNN AGRNNNGRIT VRHQGGGHKR QYRVIDFKRN KDNVAAVVKT IEYDPNRSA NIALVHYEDG VKAYILAPKG LEVGMRLVSG PEADIKVGNA LPLENIPVGT VIHNIEMKPG KGGQLIRSAG T SAQVLGKE GKYVLIRLNS GEVRMILATC RATIGSVGNE QHELINIGKA GRSRWMRKRP TVRGSVMNPN DHPHGGGEGK TP IGRKAPV SPWGQPAIGY KTRNKKAKSD KLIVRRRTK

UniProtKB: Large ribosomal subunit protein uL2

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Macromolecule #16: 50S ribosomal protein L3

MacromoleculeName: 50S ribosomal protein L3 / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Enterococcus faecalis (bacteria)
Molecular weightTheoretical: 22.758479 KDa
SequenceString: MTKGILGKKV GMTQIFTESG ELIPVTVVEA TPNVVLQVKT VETDGYEAIQ VGYQDKREVL SNKPAKGHVA KANTAPKRFI KEFKNVELG EYEVGKEIKV DVFQAGDVVD VTGTTKGKGF QGAIKRHGQS RGPMSHGSRY HRRPGSMGPV APNRVFKNKR L AGRMGGDR ...String:
MTKGILGKKV GMTQIFTESG ELIPVTVVEA TPNVVLQVKT VETDGYEAIQ VGYQDKREVL SNKPAKGHVA KANTAPKRFI KEFKNVELG EYEVGKEIKV DVFQAGDVVD VTGTTKGKGF QGAIKRHGQS RGPMSHGSRY HRRPGSMGPV APNRVFKNKR L AGRMGGDR VTIQNLEVVK VDVERNVILI KGNIPGAKKS LITIKSAVKA K

UniProtKB: Large ribosomal subunit protein uL3

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Macromolecule #17: 50S ribosomal protein L4

MacromoleculeName: 50S ribosomal protein L4 / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Enterococcus faecalis (bacteria)
Molecular weightTheoretical: 22.549717 KDa
SequenceString: MPNVALFKQD GTQNGEITLN EEIFGIEPNE SVVYDAIIMQ RASLRQGTHA VKNRSAVRGG GRKPWRQKGT GRARQGSIRS PQWRGGGVV FGPTPRSYSY KFPKKVRRLA MKSVLSDKVA ENNLVAVEGL SFDAPKTKEF KQVLANLSID TKVLVVLENG N DFAALSAR ...String:
MPNVALFKQD GTQNGEITLN EEIFGIEPNE SVVYDAIIMQ RASLRQGTHA VKNRSAVRGG GRKPWRQKGT GRARQGSIRS PQWRGGGVV FGPTPRSYSY KFPKKVRRLA MKSVLSDKVA ENNLVAVEGL SFDAPKTKEF KQVLANLSID TKVLVVLENG N DFAALSAR NLPNVSVVTS DNVSVLDVVS ANKVLATQTA LTQIEEVLA

UniProtKB: Large ribosomal subunit protein uL4

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Macromolecule #18: 50S ribosomal protein L5

MacromoleculeName: 50S ribosomal protein L5 / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Enterococcus faecalis (bacteria)
Molecular weightTheoretical: 20.12349 KDa
SequenceString:
MNRLKEKYIK EVTPSLVEKF NYSSVMQTPK VDKIVINMGV GDAVSNAKNL DKAVEELALI TGQKPLITKA KKSIAGFRLR EGMPIGAKV TLRGERMYEF LDKLVTVSLP RVRDFHGVSK KAFDGRGNYT LGIKEQLIFP EVDYDLVDKV RGMDIVIVTT A NTDEESRE LLAQLGMPFQ K

UniProtKB: Large ribosomal subunit protein uL5

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Macromolecule #19: 50S ribosomal protein L6

MacromoleculeName: 50S ribosomal protein L6 / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Enterococcus faecalis (bacteria)
Molecular weightTheoretical: 19.437303 KDa
SequenceString:
MSRIGNKVVV LPAGVEIKQD GNNITVKGPK GELTREFSSD IKMNIEGNEV TFIRPNDSKE MKTIHGTTRA NFNNMVVGVS EGFQKALEL IGVGYRAQVQ GNKLTLNVGY SHPVEMTAPE GVTFEVPANT QVIVKGINKE VVGELAANIR GVRPPEPYKG K GIRYVGEF VRRKEGKTGK

UniProtKB: Large ribosomal subunit protein uL6

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Macromolecule #20: 50S ribosomal protein L13

MacromoleculeName: 50S ribosomal protein L13 / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Enterococcus faecalis (bacteria)
Molecular weightTheoretical: 16.325902 KDa
SequenceString:
MRTTYMAKAG EVERKWYVVD ATDVPLGRLS AVVASVLRGK NKPTFTPHVD TGDFVIVINA DKVKLTGKKA TDKIYYRHSM YPGGLKSVT AGELRDKNSR RLIETSVKGM LPKNTLGRKQ FTKLNVYGGA EHPHAAQQPE VLDITNLI

UniProtKB: Large ribosomal subunit protein uL13

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Macromolecule #21: 50S ribosomal protein L14

MacromoleculeName: 50S ribosomal protein L14 / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Enterococcus faecalis (bacteria)
Molecular weightTheoretical: 13.165275 KDa
SequenceString:
MIQQESRLRV ADNSGAREIL TIKVLGGSGR KTANIGDVIV ATVKQATPGG VVKKGEVVKA VIVRTKSGAR RADGSYIKFD ENAAVIIRD DKSPRGTRIF GPVARELREN NFMKIVSLAP EVL

UniProtKB: Large ribosomal subunit protein uL14

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Macromolecule #22: 50S ribosomal protein L15

MacromoleculeName: 50S ribosomal protein L15 / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Enterococcus faecalis (bacteria)
Molecular weightTheoretical: 15.602868 KDa
SequenceString:
MKLHELKPAE GSRQVRNRVG RGTSSGNGKT AGRGQKGQKA RSGGGVRLGF EGGQTPLFRR LPKRGFTNIN RKDYAVVNLD TLNRFEDGT EVTPVVLKEA GIVKNEKAGI KVLADGELTK KLTVKAAKFS KSAQEAIEAA GGSIEVI

UniProtKB: Large ribosomal subunit protein uL15

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Macromolecule #23: 50S ribosomal protein L16

MacromoleculeName: 50S ribosomal protein L16 / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Enterococcus faecalis (bacteria)
Molecular weightTheoretical: 16.261168 KDa
SequenceString:
MLVPKRVKHR REFRGKMRGE AKGGKEVAFG EWGLQATESH WITNRQIEAA RIAMTRYMKR GGKVWIKIFP HKSYTSKAIG VRMGKGKGA PEGWVSPVKR GKIMFEIAGV PEEVAREALR LASHKLPVKT KIVKREEMGG ESNEG

UniProtKB: Large ribosomal subunit protein uL16

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Macromolecule #24: 50S ribosomal protein L17

MacromoleculeName: 50S ribosomal protein L17 / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Enterococcus faecalis (bacteria)
Molecular weightTheoretical: 14.503704 KDa
SequenceString:
MSYRKLGRTS SQRKAMLRDI TTDLIINERI VTTEARAKEV RSTVEKMITL GKRGDLHARR QAATFVRNEV ASVREEDESI VVESALQKL FNDLGPRFAE RQGGYTRILK TEPRRGDAAP MVVIEFVK

UniProtKB: Large ribosomal subunit protein bL17

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Macromolecule #25: 50S ribosomal protein L18

MacromoleculeName: 50S ribosomal protein L18 / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Enterococcus faecalis (bacteria)
Molecular weightTheoretical: 12.937829 KDa
SequenceString:
MITKPDKNKT RQKRHRRVRN KISGTAECPR LNIFRSNKNI YAQVIDDVAG VTLASASALD KEISGGTKTE TAAAVGKLVA ERAAEKGIK KVVFDRGGYL YHGRVQALAE AARENGLEF

UniProtKB: Large ribosomal subunit protein uL18

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Macromolecule #26: 50S ribosomal protein L19

MacromoleculeName: 50S ribosomal protein L19 / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Enterococcus faecalis (bacteria)
Molecular weightTheoretical: 13.244479 KDa
SequenceString:
MNPLIQELTQ EQLRTDIPAF RPGDTVRVHA KVVEGTRERI QLFEGVVIKR RGAGISETYT VRKVSNGVGV ERTFPLHTPR VAQIEVVRY GKVRRAKLYY LRALHGKAAR IKEIRR

UniProtKB: Large ribosomal subunit protein bL19

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Macromolecule #27: 50S ribosomal protein L20

MacromoleculeName: 50S ribosomal protein L20 / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Enterococcus faecalis (bacteria)
Molecular weightTheoretical: 13.674152 KDa
SequenceString:
MARVKGGTVT RKRRKKVLKL AKGYYGSKHT LFKSAKEQVM NSYYYAFRDR RQKKRDFRKL WIARINAAAR MNGLSYSKLM HGLKLAEID INRKMLADLA VNDAAAFTAL AEQAKDALSK

UniProtKB: Large ribosomal subunit protein bL20

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Macromolecule #28: 50S ribosomal protein L21

MacromoleculeName: 50S ribosomal protein L21 / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Enterococcus faecalis (bacteria)
Molecular weightTheoretical: 11.166986 KDa
SequenceString:
MYAIIKTGGK QVKVEVGQAI YVEKLNVEAG EKVVFDEVIL VGGESTKVGA PTVAGATVEG TVEKHGKQKK VVTFQYKPKK HSHRKQGHR QPYTKVMIEA INA

UniProtKB: Large ribosomal subunit protein bL21

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Macromolecule #29: 50S ribosomal protein L22

MacromoleculeName: 50S ribosomal protein L22 / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Enterococcus faecalis (bacteria)
Molecular weightTheoretical: 12.473464 KDa
SequenceString:
MSEQITSAKA TAKTVRTSPR KARLVIDLIR GKSVADAISI LKFTPNKSAG IIEKVLMSAV ANAENNFDLD VESLVVSEAF VNEGPTMKR FRPRAKGSAS PINKRTSHIT VVVTEK

UniProtKB: Large ribosomal subunit protein uL22

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Macromolecule #30: 50S ribosomal protein L23

MacromoleculeName: 50S ribosomal protein L23 / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Enterococcus faecalis (bacteria)
Molecular weightTheoretical: 11.089021 KDa
SequenceString:
MELLDVIKRP VITEKSMLAM DEKKYTFEVD TRANKTLVKQ AVESAFDVKV ANVNILNVRP KFKRMGKYAG YTKKRRKAIV TLTEDSKEI QLFEAAE

UniProtKB: Large ribosomal subunit protein uL23

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Macromolecule #31: 50S ribosomal protein L24

MacromoleculeName: 50S ribosomal protein L24 / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Enterococcus faecalis (bacteria)
Molecular weightTheoretical: 11.137063 KDa
SequenceString:
MFVKKGDKVK VITGKDKNKE GVVLAAFPKQ DKVIVEGVNV VKKHQKPNQA APQGGILEVE APIHVSNVMV IDPSNGEATK VAFKEVDGK KVRVSKKTGE VLDK

UniProtKB: Large ribosomal subunit protein uL24

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Macromolecule #32: 50S ribosomal protein L27

MacromoleculeName: 50S ribosomal protein L27 / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Enterococcus faecalis (bacteria)
Molecular weightTheoretical: 10.175572 KDa
SequenceString:
MLLTMNLQLF AHKKGGGSTS NGRDSESKRL GAKSADGQTV TGGSILYRQR GTKIYPGVNV GIGGDDTLFA KVDGVVRFER KGRDKKQVS VYPVAN

UniProtKB: Large ribosomal subunit protein bL27

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Macromolecule #33: 50S ribosomal protein L28

MacromoleculeName: 50S ribosomal protein L28 / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Enterococcus faecalis (bacteria)
Molecular weightTheoretical: 7.005314 KDa
SequenceString:
MAKVCYFTGR KTSSGNNRSH AMNSTKRTVK PNLQKVRVLI DGKPKKVWVS TRALKSGKIE RV

UniProtKB: Large ribosomal subunit protein bL28

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Macromolecule #36: 30S ribosomal protein S2

MacromoleculeName: 30S ribosomal protein S2 / type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Enterococcus faecalis (bacteria)
Molecular weightTheoretical: 29.500635 KDa
SequenceString: MAVISMKQLL EAGVHFGHQT RRWNPKMKKY IFTERNGIYI IDLQKTVKLV DAAYDYMKNV AEEGGVALFV GTKKQAQEAI KDEAIRAGQ YYVNHRWLGG TLTNWDTIQK RIARLKKINA MEEDGTFEVL PKKEVAGLNK ERERLEKFLG GIADMPRIPD V MYIVDPRK ...String:
MAVISMKQLL EAGVHFGHQT RRWNPKMKKY IFTERNGIYI IDLQKTVKLV DAAYDYMKNV AEEGGVALFV GTKKQAQEAI KDEAIRAGQ YYVNHRWLGG TLTNWDTIQK RIARLKKINA MEEDGTFEVL PKKEVAGLNK ERERLEKFLG GIADMPRIPD V MYIVDPRK ERIAVQEAHK LNIPIVAMVD TNCDPDEIDV VIPSNDDAIR AVKLITAKMA DAFIEGNQGE DQATEELFVE ET PEATSIE EIVDVVEGNN ESAE

UniProtKB: Small ribosomal subunit protein uS2

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Macromolecule #37: 30S ribosomal protein S3

MacromoleculeName: 30S ribosomal protein S3 / type: protein_or_peptide / ID: 37 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Enterococcus faecalis (bacteria)
Molecular weightTheoretical: 24.415184 KDa
SequenceString: MGQKVHPIGM RVGIIRDWDA KWYAEKEYAE FLHEDLRIRK FIATKLADAA VSTIEIERAA NRVNISIHTA KPGMVIGKGG SEVENLRKE LNKLTGKRVH INIVEIKKPD LDAKLVGEGI ARQLENRVAF RRAQKQAIQR AMRAGAKGIK TQVSGRLNGA D IARSEGYS ...String:
MGQKVHPIGM RVGIIRDWDA KWYAEKEYAE FLHEDLRIRK FIATKLADAA VSTIEIERAA NRVNISIHTA KPGMVIGKGG SEVENLRKE LNKLTGKRVH INIVEIKKPD LDAKLVGEGI ARQLENRVAF RRAQKQAIQR AMRAGAKGIK TQVSGRLNGA D IARSEGYS EGTVPLHTLR ADIDYAWEEA DTTYGKLGVK VWIYRGEILP TKKNTEKGGK

UniProtKB: Small ribosomal subunit protein uS3

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Macromolecule #38: 30S ribosomal protein S4

MacromoleculeName: 30S ribosomal protein S4 / type: protein_or_peptide / ID: 38 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Enterococcus faecalis (bacteria)
Molecular weightTheoretical: 23.273652 KDa
SequenceString: MSRYTGPSWK VSRRLGISLS GTGKELARRP YKPGQHGPNS RGKVSEYGMQ LTEKQKLRHM YGMNERQFRT LFIKASKIKE GKHGVNFMV LLEQRLDNVV YRLGLATTRR QARQLVNHGH ITVDGKRVDI PSYHVEVGQV IGVREKSQNI STIKEAVEAT V GRPAFVSF ...String:
MSRYTGPSWK VSRRLGISLS GTGKELARRP YKPGQHGPNS RGKVSEYGMQ LTEKQKLRHM YGMNERQFRT LFIKASKIKE GKHGVNFMV LLEQRLDNVV YRLGLATTRR QARQLVNHGH ITVDGKRVDI PSYHVEVGQV IGVREKSQNI STIKEAVEAT V GRPAFVSF DTEKLEGSFT RLPERDELYP EIDEALVVEY YNQKL

UniProtKB: Small ribosomal subunit protein uS4

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Macromolecule #39: 30S ribosomal protein S5

MacromoleculeName: 30S ribosomal protein S5 / type: protein_or_peptide / ID: 39 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Enterococcus faecalis (bacteria)
Molecular weightTheoretical: 17.444357 KDa
SequenceString:
MVYIDPKHLE LEDRVVAINR VTKVVKGGRR LRFAALVVVG DKNGHVGFGT GKAQEVPEAI RKAIEDAKKN LVEVPMVGST IPHEVIGVF GGGRILMKPA VEGSGVAAGG PVRAVLELAG VADITSKSLG SNTPINVVRA TVEGLKQLKR AEEVAALRGK S VEEIIG

UniProtKB: Small ribosomal subunit protein uS5

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Macromolecule #40: 30S ribosomal protein S6

MacromoleculeName: 30S ribosomal protein S6 / type: protein_or_peptide / ID: 40 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Enterococcus faecalis (bacteria)
Molecular weightTheoretical: 11.621188 KDa
SequenceString:
MSQDTKYEIM YIIRPNIDEE AKTALVERFD TILKDNGAEV IESKDWEKRR LAYEMNGFRE GIYHIVNVTS PSTAGAINEF DRLAKINDD IIRHMIVKVE A

UniProtKB: Small ribosomal subunit protein bS6

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Macromolecule #41: 30S ribosomal protein S7

MacromoleculeName: 30S ribosomal protein S7 / type: protein_or_peptide / ID: 41 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Enterococcus faecalis (bacteria)
Molecular weightTheoretical: 17.864625 KDa
SequenceString:
MPRKGPVAKR DVLPDPIYNS KLVTRLINRV MVDGKRGIAA NIIYNSFDII KESTGNDPLE VFEQAMKNVM PVLEVKARRV GGSNYQVPV EVRPERRTTL GLRWVVNYAR LRGEHTMEER LAKEIMDAAN NTGASVKKRE DTHKMADANR AFAHYRW

UniProtKB: Small ribosomal subunit protein uS7

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Macromolecule #42: 30S ribosomal protein S8

MacromoleculeName: 30S ribosomal protein S8 / type: protein_or_peptide / ID: 42 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Enterococcus faecalis (bacteria)
Molecular weightTheoretical: 14.936396 KDa
SequenceString:
MVMTDPIADF LTRIRNANMV KHETLEVPAS KIKRDIAEIL KREGFIRDVE YIEDDKQGVI RVFLKYGKNE ERVITNLKRI SKPGLRAYV KADEVPKVLN GLGIAIISTS EGVITDKEAR AKNIGGEVIA YVW

UniProtKB: Small ribosomal subunit protein uS8

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Macromolecule #43: 30S ribosomal protein S9

MacromoleculeName: 30S ribosomal protein S9 / type: protein_or_peptide / ID: 43 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Enterococcus faecalis (bacteria)
Molecular weightTheoretical: 14.27148 KDa
SequenceString:
MAQVQYSGTG RRKNAVARVR LVPGTGKITV NKKDVEEYIP HADLREVINQ PFGVTETKGA YDVIVNVNGG GYAGQSGAIR HGIARALLQ VDPDFRSALK RAGLLTRDAR MVERKKPGLK KARKASQFSK R

UniProtKB: Small ribosomal subunit protein uS9

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Macromolecule #44: 30S ribosomal protein S10

MacromoleculeName: 30S ribosomal protein S10 / type: protein_or_peptide / ID: 44 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Enterococcus faecalis (bacteria)
Molecular weightTheoretical: 11.731739 KDa
SequenceString:
MAKQKIRIRL KAYEHRILDQ SADKIVETAK RTGADVSGPI PLPTERSLYT VIRATHKYKD SREQFEMRTH KRLIDIVNPT PKTVDALMK LDLPSGVNIE IKL

UniProtKB: Small ribosomal subunit protein uS10

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Macromolecule #45: 30S ribosomal protein S11

MacromoleculeName: 30S ribosomal protein S11 / type: protein_or_peptide / ID: 45 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Enterococcus faecalis (bacteria)
Molecular weightTheoretical: 13.739913 KDa
SequenceString:
MAAKKVSRKR RVKKNIESGV AHIHSTFNNT IVMITDTHGN ALAWSSAGSL GFKGSKKSTP FAAQMAAEAA TKVAMEHGLK TVDVTVKGP GSGREAAIRS LQATGLEVTA IRDVTPVPHN GCRPPKRRRV

UniProtKB: Small ribosomal subunit protein uS11

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Macromolecule #46: 30S ribosomal protein S12

MacromoleculeName: 30S ribosomal protein S12 / type: protein_or_peptide / ID: 46 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Enterococcus faecalis (bacteria)
Molecular weightTheoretical: 15.309817 KDa
SequenceString:
MPTINQLVRK PRKSKVEKSD SPALNKGYNS FKKTQTNVNS PQKRGVCTRV GTMTPKKPNS ALRKYARVRL SNLIEVTAYI PGIGHNLQE HSVVLLRGGR VKDLPGVRYH IVRGALDTAG VNDRKQSRSK YGTKRPKA

UniProtKB: Small ribosomal subunit protein uS12

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Macromolecule #47: 30S ribosomal protein S13

MacromoleculeName: 30S ribosomal protein S13 / type: protein_or_peptide / ID: 47 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Enterococcus faecalis (bacteria)
Molecular weightTheoretical: 13.595774 KDa
SequenceString:
MARIAGVDIP RDKRVVVSLT YIYGIGNTTA KKVLANVGVS EDVRVRDLTN EQTDAIRAEI DKLKVEGDLR REVNLNIKRL MEIGSYRGI RHRRGLPTRG QNTKNNARTR KGPTKTVAGK KK

UniProtKB: Small ribosomal subunit protein uS13

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Macromolecule #48: 30S ribosomal protein S14 type Z

MacromoleculeName: 30S ribosomal protein S14 type Z / type: protein_or_peptide / ID: 48 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Enterococcus faecalis (bacteria)
Molecular weightTheoretical: 7.172593 KDa
SequenceString:
MAKKSMIAKN KRPAKHSTQA YTRCERCGRP HSVYRKFHLC RICFRELAYK GQIPGVKKAS W

UniProtKB: Small ribosomal subunit protein uS14

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Macromolecule #49: 30S ribosomal protein S15

MacromoleculeName: 30S ribosomal protein S15 / type: protein_or_peptide / ID: 49 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Enterococcus faecalis (bacteria)
Molecular weightTheoretical: 10.668236 KDa
SequenceString:
MAISQERKNE IIKEYARHEG DTGSPEVQIA VLTEDINQLN EHARTHKKDH HSYRGLMKKI GHRRNLLAYL RKTDIQRYRE LIQRLGLRR

UniProtKB: Small ribosomal subunit protein uS15

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Macromolecule #50: 30S ribosomal protein S16

MacromoleculeName: 30S ribosomal protein S16 / type: protein_or_peptide / ID: 50 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Enterococcus faecalis (bacteria)
Molecular weightTheoretical: 10.35615 KDa
SequenceString:
MAVKIRLKRM GSKKSPFYRI VVADSRSPRD GRFIETVGTY NPLKDPAEVV LKEDLVLDWL SKGAQPSDTV RNILSKEGVM KKHHEAKNV KK

UniProtKB: Small ribosomal subunit protein bS16

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Macromolecule #51: 30S ribosomal protein S17

MacromoleculeName: 30S ribosomal protein S17 / type: protein_or_peptide / ID: 51 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Enterococcus faecalis (bacteria)
Molecular weightTheoretical: 10.3321 KDa
SequenceString:
MTEERNQRKV YQGRVVSDKM DKTITVVVET KKNHPIYGKR MKYSKKYKAH DENNTAKVGD IVKIMETRPL SATKRFRLLE VVEEAVII

UniProtKB: Small ribosomal subunit protein uS17

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Macromolecule #52: 30S ribosomal protein S18

MacromoleculeName: 30S ribosomal protein S18 / type: protein_or_peptide / ID: 52 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Enterococcus faecalis (bacteria)
Molecular weightTheoretical: 9.262891 KDa
SequenceString:
MAQQRRGGRK RRKVDYIAAN HIEYIDYKDT ELLKRFISER GKILPRRVTG TGAKNQRKLT IAIKRARIMG LLPFVSDEQ

UniProtKB: Small ribosomal subunit protein bS18

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Macromolecule #53: 30S ribosomal protein S19

MacromoleculeName: 30S ribosomal protein S19 / type: protein_or_peptide / ID: 53 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Enterococcus faecalis (bacteria)
Molecular weightTheoretical: 10.586332 KDa
SequenceString:
MGRSLKKGPF VDDHLMKKVE AQQGAEKKKV IKTWSRRSTI FPSFVGFTIA VYDGRKHVPV YIQEDMVGHK LGEFAPTRTY RGHVADDKK TKR

UniProtKB: Small ribosomal subunit protein uS19

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Macromolecule #54: 30S ribosomal protein S20

MacromoleculeName: 30S ribosomal protein S20 / type: protein_or_peptide / ID: 54 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Enterococcus faecalis (bacteria)
Molecular weightTheoretical: 8.97232 KDa
SequenceString:
MPNIESAIKR VRTSANANAK NSSQTNAMRT AIKKFEEAVA AGADNVDALY NEAVKAVDMA ATKGLIHKNK ANRDKIRLSK LAK

UniProtKB: Small ribosomal subunit protein bS20

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Macromolecule #10: 23S rRNA

MacromoleculeName: 23S rRNA / type: rna / ID: 10 / Number of copies: 1
Source (natural)Organism: Enterococcus faecalis (bacteria)
Molecular weightTheoretical: 944.000625 KDa
SequenceString: GGUUAAGUGA AUAAGGGCGC ACGGUGGAUG CCUUGGCACU AGGAGCCGAU GAAGGACGGG ACUAACACCG AUAUGCUUUG GGGAGCUGU AAGUAAGCUA UGAUCCAGAG AUUUCCGAAU GGGGGAACCC AAUAUCUUUU AUAGGAUAUU ACUUUUCAGU G AAUACAUA ...String:
GGUUAAGUGA AUAAGGGCGC ACGGUGGAUG CCUUGGCACU AGGAGCCGAU GAAGGACGGG ACUAACACCG AUAUGCUUUG GGGAGCUGU AAGUAAGCUA UGAUCCAGAG AUUUCCGAAU GGGGGAACCC AAUAUCUUUU AUAGGAUAUU ACUUUUCAGU G AAUACAUA GCUGAUUAGA GGUAGACGCA GAGAACUGAA ACAUCUUAGU ACCUGCAGGA AGAGAAAGAA AAUUCGAUUC CC UGAGUAG CGGCGAGCGA AACGGGAAGA GCCCAAACCA ACAAGCUUGC UUGUUGGGGU UGUAGGACUC CGAUAUGGUA GUC UGUUAG UAUAGUUGAA GGAUUUGGAA AAUUCCGCUA AAGAGGGUGA AAGCCCCGUA GACGAAAUGC UGACAACACC UAGG AGGAU CCUGAGUACG GCGGAACACG AGAAAUUCCG UCGGAAUCCG CGGGGACCAU CCCGCAAGGC UAAAUACUCC CUAGU GACC GAUAGUGAAC CAGUACCGUG AGGGAAAGGU GAAAAGCACC CCGGAAGGGG AGUGAAAUAG AUCCUGAAAC CGUGUG CCU ACAACAAGUC AAAGCUCGUU AAUGAGUGAU GGCGUGCCUU UUGUAGAAUG AACCGGCGAG UUACGAUUGC AUGCGAG GU UAAGUCGAAG AGACGGAGCC GCAGCGAAAG CGAGUCUGAA UAGGGCGAAU GAGUAUGUAG UCGUAGACCC GAAACCAU G UGAUCUACCC AUGUCCAGGU UGAAGGUGCG GUAAAACGCA CUGGAGGACC GAACCCACGU ACGUUGAAAA GUGCGGGGA UGAGGUGUGG GUAGCGGAGA AAUUCCAAAC GAACUUGGAG AUAGCUGGUU CUCUCCGAAA UAGCUUUAGG GCUAGCCUCG GAAUUGAGA AUGAUGGAGG UAGAGCACUG UUUGGACUAG GGGCCCAUCU CGGGUUACCG AAUUCAGAUA AACUCCGAAU G CCAUUCAU UUAUAUCCGG GAGUCAGACU GCGAGUGAUA AGAUCCGUAG UCGAAAGGGA AACAGCCCAG ACCACCAGCU AA GGUCCCA AAAUAUAUGU UAAGUGGAAA AGGAUGUGGG GUUGCACAGA CAACUAGGAU GUUGGCUUAG AAGCAGCCAC CAU UUAAAG AGUGCGUAAU AGCUCACUAG UCGAGUGACC CUGCGCCGAA AAUGUACCGG GGCUAAACAU AUUACCGAAG CUGU GGACU ACACCAUUAG GUGUAGUGGU AGGAGAGCGU UCUAAGGGCG UUGAAGGUCG AUCGUGAGGA CGGCUGGAGC GCUUA GAAG UGAGAAUGCC GGUAUGAGUA GCGAAAGACA GGUGAGAAUC CUGUCCACCG UAUGACUAAG GUUUCCUGGG GAAGGC UCG UCCGCCCAGG GUUAGUCGGG ACCUAAGCCG AGGCCGAUAG GCGUAGGCGA UGGACAACAG GUUGAUAUUC CUGUACC AG UUGUUUUUGU UUGAGCAAUG GAGGGACGCA GUAGGCUAAG GAAUGCAUGC GAUUGGAAGU GCAUGUCCAA GCAAUGAG U CUUGAGUAGA GUUAAAUGCU UUACUCUUUA AGGACAAGUU GUGACGGGGA GCGAAAUAAU AGUAGCGAAG UUCCUGACG UCACACUGCC AAGAAAAGCU UCUAGUGAGA AAACAACUGC CCGUACCGUA AACCGACACA GGUAGUCGAG GAGAGUAUCC UAAGGUGAG CGAGCGAACU CUCGUUAAGG AACUCGGCAA AAUGACCCCG UAACUUCGGG AGAAGGGGUG CUGACUUCGG U CAGCCGCA GUGAAUAGGC CCAAGCGACU GUUUAUCAAA AACACAGGUC UCUGCAAAAU CGUAAGAUGA AGUAUAGGGG CU GACGCCU GCCCGGUGCU GGAAGGUUAA GAGGAUGGGU UAGCUUCGGC GAAGCUCAGA AUUGAAGCCC CAGUAAACGG CGG CCGUAA CUAUAACGGU CCUAAGGUAG CGAAAUUCCU UGUCGGGUAA GUUCCGACCC GCACGAAAGG CGUAACGAUU UGGG CACUG UCUCAACGAG AGACUCGGUG AAAUUUUAGU ACCUGUGAAG AUGCAGGUUA CCCGCGACAG GACGGAAAGA CCCCA UGGA GCUUUACUGU AGUUUGAUAU UGAGUGUUUG UACCACAUGU ACAGGAUAGG UAGGAGCCGA UGAGACCGGA ACGCUA GUU UCGGAGGAGG CGCUGGUGGG AUACUACCCU UGUGUUAUGA ACCCUCUAAC CCGCACCACU AAUCGUGGUG GGAGACA GU GUCAGAUGGG CAGUUUGACU GGGGCGGUCG CCUCCUAAAA GGUAACGGAG GCGCCCAAAG GUUCCCUCAG AAUGGUUG G AAAUCAUUCG AAGAGUGUAA AGGCAGAAGG GAGCUUGACU GCGAGACCUA CAAGUCGAGC AGGGACGAAA GUCGGGCUU AGUGAUCCGG UGGUUCCGCA UGGAAGGGCC AUCGCUCAAC GGAUAAAAGC UACCCUGGGG AUAACAGGCU UAUCUCCCCC AAGAGUCCA CAUCGACGGG GAGGUUUGGC ACCUCGAUGU CGGCUCGUCG CAUCCUGGGG CUGUAGUCGG UCCCAAGGGU U GGGCUGUU CGCCCAUUAA AGCGGCACGC GAGCUGGGUU CAGAACGUCG UGAGACAGUU CGGUCCCUAU CCGUCGCGGG CG UUGGAAA UUUGAGAGGA GCUGUCCUUA GUACGAGAGG ACCGGGAUGG ACUUACCGCU GGUGUACCAG UUGUUCUGCC AAG GGCAUU GCUGGGUAGC UAUGUAGGGA AGGGAUAAAC GCUGAAAGCA UCUAAGUGUG AAGCCCACCU CAAGAUGAGA UUUC CCAUU UCUUUAAGAA AGUAAGACCC CUGAGAGAUG AUCAGGUAGA UAGGUUGGAA GUGGAAGGCU AGUGAUAGUU GGAGC GGAC CAAUACUAAU CGGUCGAGGA CUUAACCA

GENBANK: GENBANK: CP002621.1

+
Macromolecule #11: 5S rRNA

MacromoleculeName: 5S rRNA / type: rna / ID: 11 / Number of copies: 1
Source (natural)Organism: Enterococcus faecalis (bacteria)
Molecular weightTheoretical: 37.433188 KDa
SequenceString:
UGUGGUGGCG AUAGCGAGAA GGAUACACCU GUUCCCAUGC CGAACACAGA AGUUAAGCUU CUUAGCGCCG AUUGUAGUGA AGGGUUUCC CUUUGUGAGA GUAGGACGUC GCCACGC

GENBANK: GENBANK: CP002621.1

+
Macromolecule #12: A-site tRNA

MacromoleculeName: A-site tRNA / type: rna / ID: 12
Details: Probably a mixed species, identity unknown. Fitted from Rovoz et al., 2016 (PDB 5E7K) on the basis of fit to density.
Number of copies: 1
Source (natural)Organism: Escherichia coli (E. coli)
Molecular weightTheoretical: 24.63474 KDa
SequenceString:
GAGCCGUUAG CUCAGUUGGU AGAGCAUCUG ACU(U8U)UU(T6A)A(PSU)C AGAGGGUCAC AGG(5MU)(PSU)CGAGC CCUGUACGG CUCACCA

+
Macromolecule #13: tRNA-fMet

MacromoleculeName: tRNA-fMet / type: rna / ID: 13 / Number of copies: 1
Source (natural)Organism: Enterococcus faecalis (bacteria)
Molecular weightTheoretical: 24.811795 KDa
SequenceString:
CGCGGGGUGG AGCAGUCAGG UAGCUCGUCG GGCUCAUAAC CCGAAGGUCG UAGGUUCAAA UCCUGCCCCC GCAACCA

+
Macromolecule #34: 16S rRNA

MacromoleculeName: 16S rRNA / type: rna / ID: 34 / Number of copies: 1
Source (natural)Organism: Enterococcus faecalis (bacteria)
Molecular weightTheoretical: 504.222625 KDa
SequenceString: GAGAGUUUGA UCCUGGCUCA GGACGAACGC UGGCGGCGUG CCUAAUACAU GCAAGUCGAA CGCUUCUUUC CUCCCGAGUG CUUGCACUC AAUUGGAAAG AGGAGUGGCG GACGGGUGAG UAACACGUGG GUAACCUACC CAUCAGAGGG GGAUAACACU U GGAAACAG ...String:
GAGAGUUUGA UCCUGGCUCA GGACGAACGC UGGCGGCGUG CCUAAUACAU GCAAGUCGAA CGCUUCUUUC CUCCCGAGUG CUUGCACUC AAUUGGAAAG AGGAGUGGCG GACGGGUGAG UAACACGUGG GUAACCUACC CAUCAGAGGG GGAUAACACU U GGAAACAG GUGCUAAUAC CGCAUAACAG UUUAUGCCGC AUGGCAUAAG AGUGAAAGGC GCUGGCGGGU GUCGCUGAUG GA UGGACCC GCGGUGCAUU AGCUAGUUGG UGAGGUAACG GCUCACCAAG GCCACGAUGC AUAGCCGACC UGAGAGGGUG AUC GGCCAC ACUGGGACUG AGACACGGCC CAGACUCCUA CGGGAGGCAG CAGUAGGGAA UCUUCGGCAA UGGACGAAAG UCUG ACCGA GCAACGCCGC GUGAGUGAAG AAGGUUUUCG GAUCGUAAAA CUCUGUUGUU AGAGAAGAAC AAGGACGUUA GUAAC UGAA CGUCCCCUGA CGGUAUCUAA CCAGAAAGCC ACGGCUAACU ACGUGCCAGC AGCCGCGGUA AUACGUAGGU GGCAAG CGU UGUCCGGAUU UAUUGGGCGU AAAGCGAGCG CAGGCGGUUU CUUAAGUCUG AUGUGAAAGC CCCCGGCUCA ACCGGGG AG GGUCAUUGGA AACUGGGAGA CUUGAGUGCA GAAGAGGAGA GUGGAAUUCC AUGUGUAGCG GUGAAAUGCG UAGAUAUA U GGAGGAACAC CAGUGGCGAA GGCGGCUCUC UGGUCUGUAA CUGACGCUGA GGCUCGAAAG CGUGGGGAGC AAACAGGAU UAGAUACCCU GGUAGUCCAC GCCGUAAACG AUGAGUGCUA AGUGUUGGAG GGUUUCCGCC CUUCAGUGCU GCAGCAAACG CAUUAAGCA CUCCGCCUGG GGAGUACGAC CGCAAGGUUG AAACUCAAAG GAAUUGACGG GGGCCCGCAC AAGCGGUGGA G CAUGUGGU UUAAUUCGAA GCAACGCGAA GAACCUUACC AGGUCUUGAC AUCCUUUGAC CACUCUAGAG AUAGAGCUUU CC CUUCGGG GACAAAGUGA CAGGUGGUGC AUGGUUGUCG UCAGCUCGUG UCGUGAGAUG UUGGGUUAAG UCCCGCAACG AGC GCAACC CUUAUUGUUA GUUGCCAUCA UUUAGUUGGG CACUCUAGCG AGACUGCCGG UGACAAACCG GAGGAAGGUG GGGA UGACG UCAAAUCAUC AUGCCCCUUA UGACCUGGGC UACACACGUG CUACAAUGGG AAGUACAACG AGUCGCUAGA CCGCG AGGU CAUGCAAAUC UCUUAAAGCU UCUCUCAGUU CGGAUUGCAG GCUGCAACUC GCCUGCAUGA AGCCGGAAUC GCUAGU AAU CGCGGAUCAG CACGCCGCGG UGAAUACGUU CCCGGGCCUU GUACACACCG CCCGUCACAC CACGAGAGUU UGUAACA CC CGAAGUCGGU GAGGUAACCU UUUUGGAGCC AGCCGCCUAA GGUGGGAUAG AUGAUUGGGG UGAAGUCGUA ACAAGGUA G CCGUAUCGGA AGGUGCGGCU GGAUCACCUC CUCCUCC

+
Macromolecule #35: mRNA

MacromoleculeName: mRNA / type: rna / ID: 35 / Number of copies: 1
Source (natural)Organism: Enterococcus faecalis (bacteria)
Molecular weightTheoretical: 6.243874 KDa
SequenceString:
GGAGGUAAAA AAAUGAAAA

+
Macromolecule #55: ADENOSINE-5'-TRIPHOSPHATE

MacromoleculeName: ADENOSINE-5'-TRIPHOSPHATE / type: ligand / ID: 55 / Number of copies: 2 / Formula: ATP
Molecular weightTheoretical: 507.181 Da
Chemical component information

ChemComp-ATP:
ADENOSINE-5'-TRIPHOSPHATE / ATP, energy-carrying molecule*YM

+
Macromolecule #56: MAGNESIUM ION

MacromoleculeName: MAGNESIUM ION / type: ligand / ID: 56 / Number of copies: 161 / Formula: MG
Molecular weightTheoretical: 24.305 Da

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Macromolecule #57: ZINC ION

MacromoleculeName: ZINC ION / type: ligand / ID: 57 / Number of copies: 4 / Formula: ZN
Molecular weightTheoretical: 65.409 Da

+
Macromolecule #58: POTASSIUM ION

MacromoleculeName: POTASSIUM ION / type: ligand / ID: 58 / Number of copies: 138 / Formula: K
Molecular weightTheoretical: 39.098 Da

+
Macromolecule #59: SPECTINOMYCIN

MacromoleculeName: SPECTINOMYCIN / type: ligand / ID: 59 / Number of copies: 1 / Formula: SCM
Molecular weightTheoretical: 332.35 Da
Chemical component information

ChemComp-SCM:
SPECTINOMYCIN / antibiotic*YM

+
Macromolecule #60: 1,4-DIAMINOBUTANE

MacromoleculeName: 1,4-DIAMINOBUTANE / type: ligand / ID: 60 / Number of copies: 1 / Formula: PUT
Molecular weightTheoretical: 88.151 Da
Chemical component information

ChemComp-PUT:
1,4-DIAMINOBUTANE

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Macromolecule #61: water

MacromoleculeName: water / type: ligand / ID: 61 / Number of copies: 1 / Formula: HOH
Molecular weightTheoretical: 18.015 Da
Chemical component information

ChemComp-HOH:
WATER

-
Experimental details

-
Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

-
Sample preparation

BufferpH: 9
GridModel: Quantifoil R2/2 / Material: COPPER / Mesh: 300 / Support film - Material: CARBON / Support film - topology: CONTINUOUS / Support film - Film thickness: 2 / Pretreatment - Type: GLOW DISCHARGE
VitrificationCryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 278 K / Instrument: FEI VITROBOT MARK II
DetailsGrid was prepared by four applications of elution fraction from affinity purification.

-
Electron microscopy

MicroscopeFEI TITAN KRIOS
Image recordingFilm or detector model: GATAN K2 QUANTUM (4k x 4k) / Digitization - Dimensions - Width: 3838 pixel / Digitization - Dimensions - Height: 3710 pixel / Digitization - Frames/image: 1-40 / Number grids imaged: 1 / Number real images: 3639 / Average exposure time: 5.0 sec. / Average electron dose: 30.255 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsC2 aperture diameter: 70.0 µm / Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Cs: 2.7 mm / Nominal defocus max: 1.5 µm / Nominal defocus min: 0.5 µm / Nominal magnification: 165000
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

+
Image processing

Particle selectionNumber selected: 203231
Startup modelType of model: INSILICO MODEL / In silico model: Initial model generated with RELION.
Final reconstructionResolution.type: BY AUTHOR / Resolution: 2.9 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: RELION (ver. 3.1) / Number images used: 23806
Initial angle assignmentType: RANDOM ASSIGNMENT
Final angle assignmentType: MAXIMUM LIKELIHOOD / Software - Name: RELION (ver. 3.1)
Final 3D classificationNumber classes: 6 / Software - Name: RELION (ver. 3.1)
FSC plot (resolution estimation)

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