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- EMDB-13191: Staphylococcus aureus ribosome in complex with Sal(B) -

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Basic information

Entry
Database: EMDB / ID: EMD-13191
TitleStaphylococcus aureus ribosome in complex with Sal(B)
Map dataconsensus map
Sample
  • Complex: Staphylococcus ribosome in complex with Sal(B)
    • Protein or peptide: x 48 types
    • RNA: x 5 types
  • Ligand: x 3 types
Function / homology
Function and homology information


large ribosomal subunit / ribosomal small subunit assembly / small ribosomal subunit / 5S rRNA binding / transferase activity / cytosolic small ribosomal subunit / ribosomal large subunit assembly / cytosolic large ribosomal subunit / tRNA binding / rRNA binding ...large ribosomal subunit / ribosomal small subunit assembly / small ribosomal subunit / 5S rRNA binding / transferase activity / cytosolic small ribosomal subunit / ribosomal large subunit assembly / cytosolic large ribosomal subunit / tRNA binding / rRNA binding / ribosome / structural constituent of ribosome / ribonucleoprotein complex / translation / mRNA binding / zinc ion binding / cytoplasm
Similarity search - Function
Ribosomal protein L31 type B / Type-1 KH domain profile. / Ribosomal protein L25, long-form / Ribosomal protein L25, beta domain / Ribosomal protein L25, C-terminal / Ribosomal protein TL5, C-terminal domain / : / Ribosomal protein S14, type Z / Ribosomal protein L31 signature. / Ribosomal protein L31 ...Ribosomal protein L31 type B / Type-1 KH domain profile. / Ribosomal protein L25, long-form / Ribosomal protein L25, beta domain / Ribosomal protein L25, C-terminal / Ribosomal protein TL5, C-terminal domain / : / Ribosomal protein S14, type Z / Ribosomal protein L31 signature. / Ribosomal protein L31 / Ribosomal protein L31 superfamily / Ribosomal protein L31 / : / Ribosomal protein L16 signature 1. / : / Ribosomal protein L6, conserved site / Ribosomal protein L6 signature 1. / Ribosomal protein L16, conserved site / Ribosomal protein L16 signature 2. / Ribosomal protein L17 signature. / Ribosomal L25p family / Ribosomal protein L25 / Ribosomal protein L36 signature. / Ribosomal protein L28/L24 superfamily / Ribosomal protein L25/Gln-tRNA synthetase, N-terminal / Ribosomal protein L25/Gln-tRNA synthetase, anti-codon-binding domain superfamily / Ribosomal protein L32p, bacterial type / : / : / Ribosomal protein L28 / Ribosomal protein L35, conserved site / Ribosomal protein L35 signature. / Ribosomal protein L33, conserved site / Ribosomal protein L33 signature. / Ribosomal protein L35, non-mitochondrial / Ribosomal protein L5, bacterial-type / Ribosomal protein L18, bacterial-type / Ribosomal protein L6, bacterial-type / Ribosomal protein S14/S29 / Ribosomal protein L19, conserved site / Ribosomal protein L19 signature. / Ribosomal protein S3, bacterial-type / Ribosomal protein S6, conserved site / Ribosomal protein S6 signature. / Ribosomal protein S19, bacterial-type / Ribosomal protein S13, bacterial-type / Ribosomal protein L36 / Ribosomal protein L36 superfamily / Ribosomal protein L36 / Ribosomal protein S7, bacterial/organellar-type / Ribosomal protein S11, bacterial-type / Ribosomal protein S20 / Ribosomal protein S20 superfamily / Ribosomal protein S20 / Ribosomal protein L20 signature. / Ribosomal protein S9, bacterial/plastid / Ribosomal protein L27, conserved site / Ribosomal protein L27 signature. / Ribosomal protein S4, bacterial-type / 30S ribosomal protein S17 / Ribosomal protein S5, bacterial-type / Ribosomal protein L14P, bacterial-type / Ribosomal protein S6, plastid/chloroplast / Ribosomal protein L22, bacterial/chloroplast-type / Ribosomal protein L2, bacterial/organellar-type / Ribosomal protein S2, bacteria/mitochondria/plastid / Ribosomal protein L35 / Ribosomal protein L35 superfamily / Ribosomal protein L35 / Ribosomal protein L34, conserved site / Ribosomal L28 family / Ribosomal protein L34 signature. / Ribosomal protein L33 / Ribosomal protein L33 / Ribosomal protein L28/L24 / Ribosomal protein L18 / Ribosomal L18 of archaea, bacteria, mitoch. and chloroplast / Ribosomal protein L33 superfamily / Ribosomal protein L30, bacterial-type / Ribosomal protein S18, conserved site / Ribosomal protein S18 signature. / Ribosomal protein L16 / Ribosomal protein S16 / Ribosomal protein S16 domain superfamily / Ribosomal protein S16 / : / L28p-like / Ribosomal protein L20 / Ribosomal protein S15, bacterial-type / Ribosomal protein L20 / Ribosomal protein L20, C-terminal / Ribosomal protein L21 / Ribosomal protein L27 / Ribosomal L27 protein / Ribosomal protein S6 / Ribosomal protein L19 / Ribosomal protein L19 / Ribosomal protein S6 / Ribosomal protein L19 superfamily / Ribosomal proteins 50S L24/mitochondrial 39S L24
Similarity search - Domain/homology
Large ribosomal subunit protein bL17 / Large ribosomal subunit protein uL15 / Large ribosomal subunit protein bL36 / Small ribosomal subunit protein uS14 / Small ribosomal subunit protein uS11 / Large ribosomal subunit protein uL22 / Large ribosomal subunit protein bL35 / Large ribosomal subunit protein bL28 / Small ribosomal subunit protein uS3 / Large ribosomal subunit protein uL14 ...Large ribosomal subunit protein bL17 / Large ribosomal subunit protein uL15 / Large ribosomal subunit protein bL36 / Small ribosomal subunit protein uS14 / Small ribosomal subunit protein uS11 / Large ribosomal subunit protein uL22 / Large ribosomal subunit protein bL35 / Large ribosomal subunit protein bL28 / Small ribosomal subunit protein uS3 / Large ribosomal subunit protein uL14 / Large ribosomal subunit protein bL19 / Small ribosomal subunit protein bS18 / Large ribosomal subunit protein bL33 / Large ribosomal subunit protein uL16 / Small ribosomal subunit protein uS7 / Small ribosomal subunit protein uS17 / Large ribosomal subunit protein bL20 / Large ribosomal subunit protein uL29 / Large ribosomal subunit protein bL34 / Small ribosomal subunit protein uS10 / Small ribosomal subunit protein uS19 / Small ribosomal subunit protein uS12 / Large ribosomal subunit protein bL25 / Small ribosomal subunit protein uS9 / 50S ribosomal protein L32 / Small ribosomal subunit protein uS2 / Large ribosomal subunit protein bL31B / : / 50S ribosomal protein L27 / Large ribosomal subunit protein uL2 / Large ribosomal subunit protein bL21 / Small ribosomal subunit protein bS6 / Large ribosomal subunit protein uL4 / Large ribosomal subunit protein uL24 / Large ribosomal subunit protein uL18 / Large ribosomal subunit protein uL23 / Large ribosomal subunit protein uL5 / Small ribosomal subunit protein uS5 / Large ribosomal subunit protein uL13 / Small ribosomal subunit protein uS4 / Large ribosomal subunit protein uL3 / Large ribosomal subunit protein uL6 / Small ribosomal subunit protein uS15 / Small ribosomal subunit protein bS16 / Small ribosomal subunit protein uS8 / Small ribosomal subunit protein uS13 / Large ribosomal subunit protein uL30 / Small ribosomal subunit protein bS20
Similarity search - Component
Biological speciesStaphylococcus aureus (bacteria) / Staphylococcus lentus (bacteria)
Methodsingle particle reconstruction / cryo EM / Resolution: 2.9 Å
AuthorsNicholson D / Ranson NA / O'Neill AJ
Funding support United Kingdom, 1 items
OrganizationGrant numberCountry
Wellcome Trust203743/Z/16/Z United Kingdom
Citation
Journal: Nucleic Acids Res / Year: 2022
Title: Sal-type ABC-F proteins: intrinsic and common mediators of pleuromutilin resistance by target protection in staphylococci.
Authors: Merianne Mohamad / David Nicholson / Chayan Kumar Saha / Vasili Hauryliuk / Thomas A Edwards / Gemma C Atkinson / Neil A Ranson / Alex J O'Neill /
Abstract: The first member of the pleuromutilin (PLM) class suitable for systemic antibacterial chemotherapy in humans recently entered clinical use, underscoring the need to better understand mechanisms of ...The first member of the pleuromutilin (PLM) class suitable for systemic antibacterial chemotherapy in humans recently entered clinical use, underscoring the need to better understand mechanisms of PLM resistance in disease-causing bacterial genera. Of the proteins reported to mediate PLM resistance in staphylococci, the least-well studied to date is Sal(A), a putative ABC-F NTPase that-by analogy to other proteins of this type-may act to protect the ribosome from PLMs. Here, we establish the importance of Sal proteins as a common source of PLM resistance across multiple species of staphylococci. Sal(A) is revealed as but one member of a larger group of Sal-type ABC-F proteins that vary considerably in their ability to mediate resistance to PLMs and other antibiotics. We find that specific sal genes are intrinsic to particular staphylococcal species, and show that this gene family is likely ancestral to the genus Staphylococcus. Finally, we solve the cryo-EM structure of a representative Sal-type protein (Sal(B)) in complex with the staphylococcal 70S ribosome, revealing that Sal-type proteins bind into the E site to mediate target protection, likely by displacing PLMs and other antibiotics via an allosteric mechanism.
#1: Journal: Nucleic Acids Res. / Year: 2022
Title: Sal-type ABC-F proteins: intrinsic and common mediators of pleuromutilin resistance by target protection in staphylococci
Authors: Mohamad M / Nicholson D / Saha CK / Hauryliuk V / Edwards TA / Atkinson GC / Ranson NA / O'Neill AJ
History
DepositionJul 9, 2021-
Header (metadata) releaseFeb 2, 2022-
Map releaseFeb 2, 2022-
UpdateMar 9, 2022-
Current statusMar 9, 2022Processing site: PDBe / Status: Released

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 0.065
  • Imaged by UCSF Chimera
  • Download
  • Surface view colored by height
  • Surface level: 0.065
  • Imaged by UCSF Chimera
  • Download
  • Surface view with fitted model
  • Atomic models: PDB-7p48
  • Surface level: 0.065
  • Imaged by UCSF Chimera
  • Download
Movie viewer
Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

emd_13191.png

Downloads & links

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Map

FileDownload / File: emd_13191.map.gz / Format: CCP4 / Size: 274.6 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotationconsensus map
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
1.07 Å/pix.
x 416 pix.
= 445.12 Å		1.07 Å/pix.
x 416 pix.
= 445.12 Å		1.07 Å/pix.
x 416 pix.
= 445.12 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 1.07 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.065 / Movie #1: 0.065
Minimum - Maximum-0.47836396 - 0.9345043
Average (Standard dev.)0.0008689617 (±0.017136304)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions416416416
Spacing416416416
CellA=B=C: 445.12003 Å
α=β=γ: 90.0 °

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z1.071.071.07
M x/y/z416416416
origin x/y/z0.0000.0000.000
length x/y/z445.120445.120445.120
α/β/γ90.00090.00090.000
start NX/NY/NZ000
NX/NY/NZ256256256
MAP C/R/S123
start NC/NR/NS000
NC/NR/NS416416416
D min/max/mean-0.4780.9350.001

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Supplemental data

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Mask #1

Fileemd_13191_msk_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
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Histogram

Histogram (log scale)

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Mask #2

Fileemd_13191_msk_2.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Additional map: 30S body map (after multibody refinement)

Fileemd_13191_additional_1.map
Annotation30S body map (after multibody refinement)
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Additional map: 30S head map (after multibody refinement)

Fileemd_13191_additional_2.map
Annotation30S head map (after multibody refinement)
Projections & Slices
AxesZYX

Projections

Slices (1/2)
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Histogram

Histogram (log scale)

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Half map: Half map 1

Fileemd_13191_half_map_1.map
AnnotationHalf map 1
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: Half map 2

Fileemd_13191_half_map_2.map
AnnotationHalf map 2
Projections & Slices
AxesZYX

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Slices (1/2)
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Sample components

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Entire : Staphylococcus ribosome in complex with Sal(B)

EntireName: Staphylococcus ribosome in complex with Sal(B)
Components
  • Complex: Staphylococcus ribosome in complex with Sal(B)
    • Protein or peptide: 50S ribosomal protein L33
    • Protein or peptide: 50S ribosomal protein L34
    • Protein or peptide: 50S ribosomal protein L35
    • Protein or peptide: 50S ribosomal protein L36
    • RNA: P-site tRNA
    • Protein or peptide: ABC-F type ribosomal protection protein
    • RNA: mRNA
    • RNA: 23S rRNA
    • RNA: 5S rRNA
    • Protein or peptide: 50S ribosomal protein L2
    • Protein or peptide: 50S ribosomal protein L3
    • Protein or peptide: 50S ribosomal protein L4
    • Protein or peptide: 50S ribosomal protein L5
    • Protein or peptide: 50S ribosomal protein L6
    • Protein or peptide: 50S ribosomal protein L13
    • Protein or peptide: 50S ribosomal protein L14
    • Protein or peptide: 50S ribosomal protein L15
    • Protein or peptide: 50S ribosomal protein L16
    • Protein or peptide: 50S ribosomal protein L17
    • Protein or peptide: 50S ribosomal protein L18
    • Protein or peptide: 50S ribosomal protein L19
    • Protein or peptide: 50S ribosomal protein L20
    • Protein or peptide: 50S ribosomal protein L21
    • Protein or peptide: 50S ribosomal protein L22
    • Protein or peptide: 50S ribosomal protein L23
    • Protein or peptide: 50S ribosomal protein L24
    • Protein or peptide: 50S ribosomal protein L25
    • Protein or peptide: LSU ribosomal protein L27P
    • Protein or peptide: 50S ribosomal protein L28
    • Protein or peptide: 50S ribosomal protein L29
    • Protein or peptide: 50S ribosomal protein L30
    • Protein or peptide: 50S ribosomal protein L31 type B
    • Protein or peptide: 50S ribosomal protein L32
    • RNA: 16S rRNA
    • Protein or peptide: 30S ribosomal protein S2
    • Protein or peptide: 30S ribosomal protein S3
    • Protein or peptide: 30S ribosomal protein S4
    • Protein or peptide: 30S ribosomal protein S5
    • Protein or peptide: 30S ribosomal protein S6
    • Protein or peptide: 30S ribosomal protein S7
    • Protein or peptide: 30S ribosomal protein S8
    • Protein or peptide: 30S ribosomal protein S9
    • Protein or peptide: 30S ribosomal protein S10
    • Protein or peptide: 30S ribosomal protein S11
    • Protein or peptide: 30S ribosomal protein S12
    • Protein or peptide: 30S ribosomal protein S13
    • Protein or peptide: 30S ribosomal protein S14 type Z
    • Protein or peptide: 30S ribosomal protein S15
    • Protein or peptide: 30S ribosomal protein S16
    • Protein or peptide: 30S ribosomal protein S17
    • Protein or peptide: 30S ribosomal protein S18
    • Protein or peptide: 30S ribosomal protein S19
    • Protein or peptide: 30S ribosomal protein S20
  • Ligand: ZINC ION
  • Ligand: ADENOSINE-5'-TRIPHOSPHATE
  • Ligand: MAGNESIUM ION

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Supramolecule #1: Staphylococcus ribosome in complex with Sal(B)

SupramoleculeName: Staphylococcus ribosome in complex with Sal(B) / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#53

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Macromolecule #1: 50S ribosomal protein L33

MacromoleculeName: 50S ribosomal protein L33 / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 5.944937 KDa
SequenceString:
MRVNVTLACT ECGDRNYITT KNKRNNPERI EMKKYCPRLN KYTLHRETK

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Macromolecule #2: 50S ribosomal protein L34

MacromoleculeName: 50S ribosomal protein L34 / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 5.454642 KDa
SequenceString:
MVKRTYQPNK RKHSKVHGFR KRMSTKNGRK VLARRRRKGR KVLSA

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Macromolecule #3: 50S ribosomal protein L35

MacromoleculeName: 50S ribosomal protein L35 / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 7.722368 KDa
SequenceString:
MPKMKTHRGA AKRVKRTASG QLKRSRAFTS HLFANKSTKQ KRQLRKARLV SKSDMKRVKQ LLAYKK

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Macromolecule #4: 50S ribosomal protein L36

MacromoleculeName: 50S ribosomal protein L36 / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 4.318422 KDa
SequenceString:
MKVRPSVKPI CEKCKVIKRK GKVMVICENP KHKQRQG

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Macromolecule #6: ABC-F type ribosomal protection protein

MacromoleculeName: ABC-F type ribosomal protection protein / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus lentus (bacteria)
Molecular weightTheoretical: 66.070578 KDa
Recombinant expressionOrganism: Staphylococcus aureus (bacteria)
SequenceString: MLFLFEEKPL EIENKQLIKR LSFHIEDHEH LALIGVNGIG KSTLLHHIHK NELIDTAMME QDLSKHDDID VMDYVMSAYP KLVELRKDL SDIDSLNSYI ELDGYNVENN IIIEGNKLGL SSTHFEQKIG TLSGGEQTKV SFLKVILSDA PLLLIDQPTN H MDKEMKVW ...String:
MLFLFEEKPL EIENKQLIKR LSFHIEDHEH LALIGVNGIG KSTLLHHIHK NELIDTAMME QDLSKHDDID VMDYVMSAYP KLVELRKDL SDIDSLNSYI ELDGYNVENN IIIEGNKLGL SSTHFEQKIG TLSGGEQTKV SFLKVILSDA PLLLIDQPTN H MDKEMKVW LIKAFKSEQR AILFVSHDRE FLNETPDAIL ELTKDGATRY SGHYDDYKNQ KDIEIETEKL KYEKEQKEQK AI EESIKKY KEWYQRAAQK ASVRSPYAQK QLSKLAKRFK SKEHQLNRKL EESKSDNPLE ENKSFSIENN EFKSHYLVRF ENV SFSYKS REIFKDTYFE IKRNQTVIIE GKNGSGKSTL IQLILGNLLP MSGAVKKHPD LDIGYFSQDF QNLNPNNSVL EEVM DIENM MITDARTILA SFYFDKSRMN DKVRQLSMGE KCRLQFVKLY FSNPHILILD QPTNYFDISM QEKIIQLIQS FNGAV IIVS HDEIFKDEIR DQVWKIENCK LIHENVSINT PIDAESMKDE LKILEQYTDE RNKETDFGGD YKDHDGDYKD HDIDYK DDD DK

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Macromolecule #10: 50S ribosomal protein L2

MacromoleculeName: 50S ribosomal protein L2 / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 30.087984 KDa
SequenceString: MAIKKYKPIT NGRRNMTSLD FAEITKTTPE KSLLKPLPKK AGRNNQGKLT VRHHGGGHKR QYRVIDFKRN KDGINAKVDS IQYDPNRSA NIALVVYADG EKRYIIAPKG LEVGQIVESG AEADIKVGNA LPLQNIPVGT VVHNIELKPG KGGQIARSAG A SAQVLGKE ...String:
MAIKKYKPIT NGRRNMTSLD FAEITKTTPE KSLLKPLPKK AGRNNQGKLT VRHHGGGHKR QYRVIDFKRN KDGINAKVDS IQYDPNRSA NIALVVYADG EKRYIIAPKG LEVGQIVESG AEADIKVGNA LPLQNIPVGT VVHNIELKPG KGGQIARSAG A SAQVLGKE GKYVLIRLRS GEVRMILSTC RATIGQVGNL QHELVNVGKA GRSRWKGIRP TVRGSVMNPN DHPHGGGEGR AP IGRPSPM SPWGKPTLGK KTRRGKKSSD KLIVRGRKK

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Macromolecule #11: 50S ribosomal protein L3

MacromoleculeName: 50S ribosomal protein L3 / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 23.760256 KDa
SequenceString: MTKGILGRKI GMTQVFGENG ELIPVTVVEA KENVVLQKKT VEVDGYNAIQ VGFEDKKAYK KDAKSNKYAN KPAEGHAKKA DAAPKRFIR EFRNVDVDAY EVGQEVSVDT FVAGDVIDVT GVSKGKGFQG AIKRHGQSRG PMSHGSHFHR APGSVGMASD A SRVFKGQK ...String:
MTKGILGRKI GMTQVFGENG ELIPVTVVEA KENVVLQKKT VEVDGYNAIQ VGFEDKKAYK KDAKSNKYAN KPAEGHAKKA DAAPKRFIR EFRNVDVDAY EVGQEVSVDT FVAGDVIDVT GVSKGKGFQG AIKRHGQSRG PMSHGSHFHR APGSVGMASD A SRVFKGQK MPGRMGGNTV TVQNLEVVQV DTENKVILVK GNVPGPKKGL VEIRTSIKKG NK

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Macromolecule #12: 50S ribosomal protein L4

MacromoleculeName: 50S ribosomal protein L4 / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 22.495697 KDa
SequenceString: MANYDVLKLD GTKSGSIELS DAVFGIEPNN SVLFEAINLQ RASLRQGTHA VKNRSAVSGG GRKPWKQKGT GRARQGTIRA PQWRGGGIV FGPTPRSYAY KMPKKMRRLA LRSALSFKAQ ENGLTVVDAF NFEAPKTKEF KNVLSTLEQP KKVLVVTENE D VNVELSAR ...String:
MANYDVLKLD GTKSGSIELS DAVFGIEPNN SVLFEAINLQ RASLRQGTHA VKNRSAVSGG GRKPWKQKGT GRARQGTIRA PQWRGGGIV FGPTPRSYAY KMPKKMRRLA LRSALSFKAQ ENGLTVVDAF NFEAPKTKEF KNVLSTLEQP KKVLVVTENE D VNVELSAR NIPGVQVTTA QGLNVLDITN ADSLVITEAA AKKVEEVLG

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Macromolecule #13: 50S ribosomal protein L5

MacromoleculeName: 50S ribosomal protein L5 / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 20.296637 KDa
SequenceString:
MNRLKEKFNT EVTENLMKKF NYSSVMEVPK IDKIVVNMGV GDAVQNSKVL DNAVEELELI TGQKPLVTKA KKSIATFRLR EGMPIGAKV TLRGERMYEF LDKLISVSLP RVRDFQGVSK KAFDGRGNYT LGVKEQLIFP EIDYDKVSKV RGMDIVIVTT A NTDEEARE LLANFGMPFR K

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Macromolecule #14: 50S ribosomal protein L6

MacromoleculeName: 50S ribosomal protein L6 / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 19.81858 KDa
SequenceString:
MSRVGKKIID IPSDVTVTFD GNHVTVKGPK GELSRTLNER MTFKQEENTI EVVRPSDSKE DRTNHGTTRA LLNNMVQGVS QGYVKVLEL VGVGYRAQMQ GKDLILNVGY SHPVEIKAEE NITFSVEKNT VVKVEGISKE QVGALASNIR SVRPPEPYKG K GIRYQGEY VRRKEGKTGK

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Macromolecule #15: 50S ribosomal protein L13

MacromoleculeName: 50S ribosomal protein L13 / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 16.359427 KDa
SequenceString:
MRQTFMANES NIERKWYVID AEGQTLGRLS SEVASILRGK NKVTYTPHVD TGDYVIVINA SKIEFTGNKE TDKVYYRHSN HPGGIKSIT AGELRRTNPE RLIENSIKGM LPSTRLGEKQ GKKLFVYGGA EHPHAAQQPE NYELRG

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Macromolecule #16: 50S ribosomal protein L14

MacromoleculeName: 50S ribosomal protein L14 / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 13.157342 KDa
SequenceString:
MIQQETRLKV ADNSGAREVL TIKVLGGSGR KTANIGDVIV CTVKNATPGG VVKKGDVVKA VIVRTKSGVR RNDGSYIKFD ENACVIIRD DKGPRGTRIF GPVARELREG NFMKIVSLAP EVL

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Macromolecule #17: 50S ribosomal protein L15

MacromoleculeName: 50S ribosomal protein L15 / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 15.62889 KDa
SequenceString:
MKLHELKPAE GSRKERNRVG RGVATGNGKT SGRGHKGQKA RSGGGVRPGF EGGQLPLFRR LPKRGFTNIN RKEYAIVNLD QLNKFEDGT EVTPALLVES GVVKNEKSGI KILGNGSLDK KLTVKAHKFS ASAAEAIDAK GGAHEVI

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Macromolecule #18: 50S ribosomal protein L16

MacromoleculeName: 50S ribosomal protein L16 / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 16.274049 KDa
SequenceString:
MLLPKRVKYR RQHRPKTTGR SKGGNYVTFG EFGLQATTTS WITSRQIESA RIAMTRYMKR GGKVWIKIFP HTPYTKKPLE VRMGAGKGA VEGWIAVVKP GRILFEVAGV SEEVAREALR LASHKLPVKT KFVKREELGG ETNES

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Macromolecule #19: 50S ribosomal protein L17

MacromoleculeName: 50S ribosomal protein L17 / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 13.771773 KDa
SequenceString:
MGYRKLGRTS DQRKAMLRDL ATSLIISERI ETTEARAKEV RSVVEKLITL GKKGDLASRR NAAKTLRNVE ILNEDETTQT ALQKLFGEI AERYTERQGG YTRILKQGPR RGDGAESVII ELV

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Macromolecule #20: 50S ribosomal protein L18

MacromoleculeName: 50S ribosomal protein L18 / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 13.124093 KDa
SequenceString:
MISKIDKNKV RLKRHARVRT NLSGTAEKPR LNVYRSNKHI YAQIIDDNKG VTLAQASSKD SDIATTATKV ELATKVGEAI AKKAADKGI KEIVFDRGGY LYHGRVKALA EAARESGLEF

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Macromolecule #21: 50S ribosomal protein L19

MacromoleculeName: 50S ribosomal protein L19 / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 13.392771 KDa
SequenceString:
MTNHKLIEAV TKSQLRTDLP SFRPGDTLRV HVRIIEGTRE RIQVFEGVVI KRRGGGVSET FTVRKISSGV GVERTFPLHT PKIEKIEVK RRGKVRRAKL YYLRSLRGKA ARIQEIR

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Macromolecule #22: 50S ribosomal protein L20

MacromoleculeName: 50S ribosomal protein L20 / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 13.720295 KDa
SequenceString:
MPRVKGGTVT RARRKKTIKL AKGYFGSKHT LYKVAKQQVM KSGQYAFRDR RQRKRDFRKL WITRINAAAR QHEMSYSRLM NGLKKAGID INRKMLSEIA ISDEKAFAQL VTKAKDALK

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Macromolecule #23: 50S ribosomal protein L21

MacromoleculeName: 50S ribosomal protein L21 / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 11.354081 KDa
SequenceString:
MFAIIETGGK QIKVEEGQEI FVEKLDVNEG DTFTFDKVLF VGGDSVKVGA PTVEGATVTA TVNKQGRGKK ITVFTYKRRK NSKRKKGHR QPYTKLTIDK INA

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Macromolecule #24: 50S ribosomal protein L22

MacromoleculeName: 50S ribosomal protein L22 / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 12.857922 KDa
SequenceString:
MEAKAVARTI RIAPRKVRLV LDLIRGKNAA EAIAILKLTN KASSPVIEKV LMSALANAEH NYDMNTDELV VKEAYANEGP TLKRFRPRA QGRASAINKR TSHITIVVSD GKEEAKEA

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Macromolecule #25: 50S ribosomal protein L23

MacromoleculeName: 50S ribosomal protein L23 / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 10.625398 KDa
SequenceString:
MEARDILKRP VITEKSSEAM AEDKYTFDVD TRVNKTQVKM AVEEIFNVKV ASVNIMNYKP KKKRMGRYQG YTNKRRKAIV TLKEGSIDL FN

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Macromolecule #26: 50S ribosomal protein L24

MacromoleculeName: 50S ribosomal protein L24 / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 11.561504 KDa
SequenceString:
MHIKKGDNVK VIAGKDKGKE GKVIATLPKK DRVVVEGVNI MKKHQKPTQL NPEGGILETE AAIHVSNVQL LDPKTNEPTR VGYKFVDGK KVRIAKKSGE EIKSNN

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Macromolecule #27: 50S ribosomal protein L25

MacromoleculeName: 50S ribosomal protein L25 / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 24.098998 KDa
SequenceString: MRMASLKSII RQGKQTRSDL KQLRKSGKVP AVVYGYGTKN VSVKVDEVEF IKVIREVGRN GVIELGVGSK TIKVMVADYQ FDPLKNQIT HIDFLAINMS EERTVEVPVQ LVGEAVGAKE GGVVEQPLFN LEVTATPDNI PEAIEVDITE LNINDSLTVA D VKVTGDFK ...String:
MRMASLKSII RQGKQTRSDL KQLRKSGKVP AVVYGYGTKN VSVKVDEVEF IKVIREVGRN GVIELGVGSK TIKVMVADYQ FDPLKNQIT HIDFLAINMS EERTVEVPVQ LVGEAVGAKE GGVVEQPLFN LEVTATPDNI PEAIEVDITE LNINDSLTVA D VKVTGDFK IENDSAESVV TVVAPTEEPT EEEIEAMEGE QQTEEPEVVG ESKEDEEKTE E

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Macromolecule #28: LSU ribosomal protein L27P

MacromoleculeName: LSU ribosomal protein L27P / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 10.334798 KDa
SequenceString:
MLKLNLQFFA SKKGVSSTKN GRDSESKRLG AKRADGQFVT GGSILYRQRG TKIYPGENVG RGGDDTLFAK IDGVVKFERK GRDKKQVSV YAVAE

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Macromolecule #29: 50S ribosomal protein L28

MacromoleculeName: 50S ribosomal protein L28 / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 6.995289 KDa
SequenceString:
MGKQCFVTGR KASTGNRRSH ALNSTKRRWN ANLQKVRILV DGKPKKVWVS ARALKSGKVT RV

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Macromolecule #30: 50S ribosomal protein L29

MacromoleculeName: 50S ribosomal protein L29 / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 8.105266 KDa
SequenceString:
MKAKEIRDLT TSEIEEQIKS SKEELFNLRF QLATGQLEET ARIRTVRKTI ARLKTVARER EIEQSKANQ

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Macromolecule #31: 50S ribosomal protein L30

MacromoleculeName: 50S ribosomal protein L30 / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 6.565683 KDa
SequenceString:
MAKLQITLTR SVIGRPETQR KTVEALGLKK TNSSVVVEDN PAIRGQINKV KHLVTVEEK

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Macromolecule #32: 50S ribosomal protein L31 type B

MacromoleculeName: 50S ribosomal protein L31 type B / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 14.311188 KDa
SequenceString:
MKQGIHPEYH QVIFLDTTTN FKFLSGSTKT SSEMMEWEDG KEYPVIRLDI SSDSHPFYTG RQKFAAADGR VERFNKKFGL NQTTNIVIV KLSASYNGIV DSFLFISVVR VYTYDCVQRS DSIPCC

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Macromolecule #33: 50S ribosomal protein L32

MacromoleculeName: 50S ribosomal protein L32 / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 6.544663 KDa
SequenceString:
MAVPKRRTSK TRKNKRRTHF KISVPGMTEC PNCGEYKLSH RVCKNCGSYN GEEVVSK

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Macromolecule #35: 30S ribosomal protein S2

MacromoleculeName: 30S ribosomal protein S2 / type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 26.588031 KDa
SequenceString: MAVISMKQLL EAGVHFGHQT RRWNPKMKKY IFTERNGIYI IDLQKTVKKV DEAYNFLKQV SEDGGQVLFV GTKKQAQESV KSEAERAGQ FYINQRWLGG LLTNYKTISK RIKRISEIEK MEEDGLFEVL PKKEVVELKK EYDRLIKFLG GIRDMKSMPQ A LFVVDPRK ...String:
MAVISMKQLL EAGVHFGHQT RRWNPKMKKY IFTERNGIYI IDLQKTVKKV DEAYNFLKQV SEDGGQVLFV GTKKQAQESV KSEAERAGQ FYINQRWLGG LLTNYKTISK RIKRISEIEK MEEDGLFEVL PKKEVVELKK EYDRLIKFLG GIRDMKSMPQ A LFVVDPRK ERNAIAEARK LNIPIVGIVD TNCDPDEIDY VIPANDDAIR AVKLLTAKMA DAILEGQQGV LMKK

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Macromolecule #36: 30S ribosomal protein S3

MacromoleculeName: 30S ribosomal protein S3 / type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 24.143867 KDa
SequenceString: MGQKINPIGL RVGIIRDWEA KWYAEKDFAS LLHEDLKIRK FIDNELKEAS VSHVEIERAA NRINIAIHTG KPGMVIGKGG SEIEKLRNK LNALTDKKVH INVIEIKKVD LDARLVAENI ARQLENRASF RRVQKQAITR AMKLGAKGIK TQVSGRLGGA D IARAEQYS ...String:
MGQKINPIGL RVGIIRDWEA KWYAEKDFAS LLHEDLKIRK FIDNELKEAS VSHVEIERAA NRINIAIHTG KPGMVIGKGG SEIEKLRNK LNALTDKKVH INVIEIKKVD LDARLVAENI ARQLENRASF RRVQKQAITR AMKLGAKGIK TQVSGRLGGA D IARAEQYS EGTVPLHTLR ADIDYAHAEA DTTYGKLGVK VWIYRGEVLP TKNTSGGGK

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Macromolecule #37: 30S ribosomal protein S4

MacromoleculeName: 30S ribosomal protein S4 / type: protein_or_peptide / ID: 37 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 23.051416 KDa
SequenceString: MARFRGSNWK KSRRLGISLS GTGKELEKRP YAPGQHGPNQ RKKLSEYGLQ LREKQKLRYL YGMTERQFRN TFDIAGKKFG VHGENFMIL LASRLDAVVY SLGLARTRRQ ARQLVNHGHI LVDGKRVDIP SYSVKPGQTI SVREKSQKLN IIVESVEINN F VPEYLNFD ...String:
MARFRGSNWK KSRRLGISLS GTGKELEKRP YAPGQHGPNQ RKKLSEYGLQ LREKQKLRYL YGMTERQFRN TFDIAGKKFG VHGENFMIL LASRLDAVVY SLGLARTRRQ ARQLVNHGHI LVDGKRVDIP SYSVKPGQTI SVREKSQKLN IIVESVEINN F VPEYLNFD ADSLTGTFVR LPERSELPAE INEQLIVEYY SR

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Macromolecule #38: 30S ribosomal protein S5

MacromoleculeName: 30S ribosomal protein S5 / type: protein_or_peptide / ID: 38 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 17.770512 KDa
SequenceString:
MARREEETKE FEERVVTINR VAKVVKGGRR FRFTALVVVG DKNGRVGFGT GKAQEVPEAI KKAVEAAKKD LVVVPRVEGT TPHTITGRY GSGSVFMKPA APGTGVIAGG PVRAVLELAG ITDILSKSLG SNTPINMVRA TIDGLQNLKN AEDVAKLRGK T VEELYN

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Macromolecule #39: 30S ribosomal protein S6

MacromoleculeName: 30S ribosomal protein S6 / type: protein_or_peptide / ID: 39 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 11.613146 KDa
SequenceString:
MRTYEVMYIV RPNIEEDAKK ALVERFNGIL ATEGAEVLEA KDWGKRRLAY EINDFKDGFY NIVRVKSDNN KATDEFQRLA KISDDIIRY MVIREDEDK

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Macromolecule #40: 30S ribosomal protein S7

MacromoleculeName: 30S ribosomal protein S7 / type: protein_or_peptide / ID: 40 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 17.826555 KDa
SequenceString:
MPRKGSVPKR DVLPDPIHNS KLVTKLINKI MLDGKRGTAQ RILYSAFDLV EQRSGRDALE VFEEAINNIM PVLEVKARRV GGSNYQVPV EVRPERRTTL GLRWLVNYAR LRGEKTMEDR LANEILDAAN NTGGAVKKRE DTHKMAEANK AFAHYRW

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Macromolecule #41: 30S ribosomal protein S8

MacromoleculeName: 30S ribosomal protein S8 / type: protein_or_peptide / ID: 41 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 14.854315 KDa
SequenceString:
MTMTDPIADM LTRVRNANMV RHEKLELPAS NIKKEIAEIL KSEGFIKNVE YVEDDKQGVL RLFLKYGQND ERVITGLKRI SKPGLRVYA KASEMPKVLN GLGIALVSTS EGVITDKEAR KRNVGGEIIA YVW

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Macromolecule #42: 30S ribosomal protein S9

MacromoleculeName: 30S ribosomal protein S9 / type: protein_or_peptide / ID: 42 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 14.642725 KDa
SequenceString:
MAQVEYRGTG RRKNSVARVR LVPGEGNITV NNRDVREYLP FESLILDLNQ PFDVTETKGN YDVLVNVHGG GFTGQAQAIR HGIARALLE ADPEYRGSLK RAGLLTRDPR MKERKKPGLK AARRSPQFSK R

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Macromolecule #43: 30S ribosomal protein S10

MacromoleculeName: 30S ribosomal protein S10 / type: protein_or_peptide / ID: 43 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 11.598503 KDa
SequenceString:
MAKQKIRIRL KAYDHRVIDQ SAEKIVETAK RSGADVSGPI PLPTEKSVYT IIRAVHKYKD SREQFEQRTH KRLIDIVNPT PKTVDALMG LNLPSGVDIE IKL

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Macromolecule #44: 30S ribosomal protein S11

MacromoleculeName: 30S ribosomal protein S11 / type: protein_or_peptide / ID: 44 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 13.907978 KDa
SequenceString:
MARKQVSRKR RVKKNIENGV AHIRSTFNNT IVTITDEFGN ALSWSSAGAL GFKGSKKSTP FAAQMASETA SKSAMEHGLK TVEVTVKGP GPGRESAIRA LQSAGLEVTA IRDVTPVPHN GCRPPKRRRV

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Macromolecule #45: 30S ribosomal protein S12

MacromoleculeName: 30S ribosomal protein S12 / type: protein_or_peptide / ID: 45 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 16.813574 KDa
SequenceString:
MGLKSEERRT YHMPTINQLV RKPRQSKIKK SDSPALNKGF NSKKKKFTDL NSPQKRGVCT RVGTMTPKKP NSALRKYARV RLSNNIEIN AYIPGIGHNL QEHSVVLVRG GRVKDLPGVR YHIVRGALDT SGVDGRRQGR SLYGTKKPKN

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Macromolecule #46: 30S ribosomal protein S13

MacromoleculeName: 30S ribosomal protein S13 / type: protein_or_peptide / ID: 46 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 13.747919 KDa
SequenceString:
MARIAGVDIP REKRVVISLT YIYGIGTSTA QKILEEANVS ADTRVKDLTD DELGRIREVV DGYKVEGDLR RETNLNIKRL MEISSYRGI RHRRGLPVRG QKTKNNARTR KGPVKTVANK KK

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Macromolecule #47: 30S ribosomal protein S14 type Z

MacromoleculeName: 30S ribosomal protein S14 type Z / type: protein_or_peptide / ID: 47 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 7.317769 KDa
SequenceString:
MAKTSMVAKQ QKKQKYAVRE YTRCERCGRP HSVYRKFKLC RICFRELAYK GQIPGVRKAS W

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Macromolecule #48: 30S ribosomal protein S15

MacromoleculeName: 30S ribosomal protein S15 / type: protein_or_peptide / ID: 48 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 10.63433 KDa
SequenceString:
MAISQERKNE IIKEYRVHET DTGSPEVQIA VLTAEINAVN EHLRTHKKDH HSRRGLLKMV GRRRHLLNYL RSKDIQRYRE LIKSLGIRR

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Macromolecule #49: 30S ribosomal protein S16

MacromoleculeName: 30S ribosomal protein S16 / type: protein_or_peptide / ID: 49 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 10.253886 KDa
SequenceString:
MAVKIRLTRL GSKRNPFYRI VVADARSPRD GRIIEQIGTY NPTSANAPEI KVDEALALKW LNDGAKPTDT VHNILSKEGI MKKFDEQKK AK

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Macromolecule #50: 30S ribosomal protein S17

MacromoleculeName: 30S ribosomal protein S17 / type: protein_or_peptide / ID: 50 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 10.196888 KDa
SequenceString:
MSERNDRKVY VGKVVSDKMD KTITVLVETY KTHKLYGKRV KYSKKYKTHD ENNSAKLGDI VKIQETRPLS ATKRFRLVEI VEESVII

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Macromolecule #51: 30S ribosomal protein S18

MacromoleculeName: 30S ribosomal protein S18 / type: protein_or_peptide / ID: 51 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 9.332018 KDa
SequenceString:
MAGGPRRGGR RRKKVCYFTA NGITHIDYKD TELLKRFISE RGKILPRRVT GTSAKYQRML TTAIKRSRHM ALLPYVKEEQ

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Macromolecule #52: 30S ribosomal protein S19

MacromoleculeName: 30S ribosomal protein S19 / type: protein_or_peptide / ID: 52 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 12.31934 KDa
SequenceString:
MCGLKLHAHN KKGGAQMARS IKKGPFVDEH LMKKVEAQEG SEKKQVIKTW SRRSTIFPNF IGHTFAVYDG RKHVPVYVTE DMVGHKLGE FAPTRTFKGH VADDKKTRR

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Macromolecule #53: 30S ribosomal protein S20

MacromoleculeName: 30S ribosomal protein S20 / type: protein_or_peptide / ID: 53 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 9.039472 KDa
SequenceString:
MANIKSAIKR VKTTEKAEAR NISQKSAMRT AVKNAKTAVS NNADNKNELV SLAVKLVDKA AQSNLIHSNK ADRIKSQLMT ANK

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Macromolecule #5: P-site tRNA

MacromoleculeName: P-site tRNA / type: rna / ID: 5 / Number of copies: 1
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 23.544002 KDa
SequenceString:
GCGGGAUGGA GCAGUUCGGU AGCUCGUCGG GCUCAUAACC CGAAGGUCGG UGGUUCAAAU CCGCCUCCCG CAA

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Macromolecule #7: mRNA

MacromoleculeName: mRNA / type: rna / ID: 7 / Number of copies: 1
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 935.62 Da
SequenceString:
AUG

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Macromolecule #8: 23S rRNA

MacromoleculeName: 23S rRNA / type: rna / ID: 8 / Number of copies: 1
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 946.022188 KDa
SequenceString: UUAAGUUAUU AAGGGCGCAC GGUGGAUGCC UUGGCACUAG AAGCCGAUGA AGGACGUUAC UAACGACGAU AUGCUUUGGG GAGCUGUAA GUAAGCUUUG AUCCAGAGAU UUCCGAAUGG GGAAACCCAG CAUGAGUUAU GUCAUGUUAU CGAUAUGUGA A UACAUAGC ...String:
UUAAGUUAUU AAGGGCGCAC GGUGGAUGCC UUGGCACUAG AAGCCGAUGA AGGACGUUAC UAACGACGAU AUGCUUUGGG GAGCUGUAA GUAAGCUUUG AUCCAGAGAU UUCCGAAUGG GGAAACCCAG CAUGAGUUAU GUCAUGUUAU CGAUAUGUGA A UACAUAGC AUAUCAGAAG GCACACCCGG AGAACUGAAA CAUCUUAGUA CCCGGAGGAA GAGAAAGAAA AUUCGAUUCC CU UAGUAGC GGCGAGCGAA AUGGGAAGAG CCCAAACCAA CAAGCUUGCU UGUUGGGGUU GUAGGACACU CUAUACGGAG UUA CAAAGG ACGACAUUAG ACGAAUCAUC UGGAAAGAUG AAUCAAAGAA GGUAAUAAUC CUGUAGUCGA AAAUGUUGUC UCUC UUGAG UGGAUCCUGA GUACGACGGA GCACGUGAAA UUCCGUCGGA AUCUGGGAGG ACCAUCUCCU AAGGCUAAAU ACUCU CUAG UGACCGAUAG UGAACCAGUA CCGUGAGGGA AAGGUGAAAA GCACCCCGGA AGGGGAGUGA AAUAGAACCU GAAACC GUG UGCUUACAAG UAGUCAGAGC CCGUUAAUGG GUGAUGGCGU GCCUUUUGUA GAAUGAACCG GCGAGUUACG AUUUGAU GC AAGGUUAAGC AGUAAAUGUG GAGCCGUAGC GAAAGCGAGU CUGAAUAGGG CGUUUAGUAU UUGGUCGUAG ACCCGAAA C CAGGUGAUCU ACCCUUGGUC AGGUUGAAGU UCAGGUAACA CUGAAUGGAG GACCGAACCG ACUUACGUUG AAAAGUGAG CGGAUGAACU GAGGGUAGCG GAGAAAUUCC AAUCGAACCU GGAGAUAGCU GGUUCUCUCC GAAAUAGCUU UAGGGCUAGC CUCAAGUGA UGAUUAUUGG AGGUAGAGCA CUGUUUGGAC GAGGGGCCCC UCUCGGGUUA CCGAAUUCAG ACAAACUCCG A AUGCCAAU UAAUUUAACU UGGGAGUCAG AACAUGGGUG AUAAGGUCCG UGUUCGAAAG GGAAACAGCC CAGACCACCA GC UAAGGCC CCAAAAUAUA UGUUAAGUGG AAAAGGAUGU GGCGUUGCCC AGACAACUAG GAUGUUGGCU UAGAAGCAGC CAU CAUUUA AAGAGUGCGU AAUAGCUCAC UAGUCGAGUG ACACUGCGCC GAAAAUGUAC CGGGGCUAAA CAUAUUACCG AAGC UGUGG AUUGUCCUUU GGACAAUGGU AGGAGAGCGU UCUAAGGGCG UUGAAGCAUG AUCGUAAGGA CAUGUGGAGC GCUUA GAAG UGAGAAUGCC GGUGUGAGUA GCGAAAGACG GGUGAGAAUC CCGUCCACCG AUUGACUAAG GUUUCCAGAG GAAGGC UCG UCCGCUCUGG GUUAGUCGGG UCCUAAGCUG AGGCCGACAG GCGUAGGCGA UGGAUAACAG GUUGAUAUUC CUGUACC AC CUAUAAUCGU UUUAAUCGAU GGGGGGACGC AGUAGGAUAG GCGAAGCGUG CGAUUGGAUU GCACGUCUAA GCAGUAAG G CUGAGUAUUA GGCAAAUCCG GUACUCGUUA AGGCUGAGCU GUGAUGGGGA GAAGACAUUG UGUCUUCGAG UCGUUGAUU UCACACUGCC GAGAAAAGCC UCUAGAUAGA AAAUAGGUGC CCGUACCGCA AACCGACACA GGUAGUCAAG AUGAGAAUUC UAAGGUGAG CGAGCGAACU CUCGUUAAGG AACUCGGCAA AAUGACCCCG UAACUUCGGG AGAAGGGGUG CUCUUUAGGG U UAACGCCC AGAAGAGCCG CAGUGAAUAG GCCCAAGCGA CUGUUUAUCA AAAACACAGG UCUCUGCUAA ACCGUAAGGU GA UGUAUAG GGGCUGACGC CUGCCCGGUG CUGGAAGGUU AAGAGGAGUG GUUAGCUUCU GCGAAGCUAC GAAUCGAAGC CCC AGUAAA CGGCGGCCGU AACUAUAACG GUCCUAAGGU AGCGAAAUUC CUUGUCGGGU AAGUUCCGAC CCGCACGAAA GGCG UAACG AUUUGGGCAC UGUCUCAACG AGAGACUCGG UGAAAUCAUA GUACCUGUGA AGAUGCAGGU UACCCGCGAC AGGAC GGAA AGACCCCGUG GAGCUUUACU GUAGCCUGAU AUUGAAAUUC GGCACAGCUU GUACAGGAUA GGUAGGAGCC UUUGAA ACG UGAGCGCUAG CUUACGUGGA GGCGCUGGUG GGAUACUACC CUAGCUGUGU UGGCUUUCUA ACCCGCACCA CUUAUCG UG GUGGGAGACA GUGUCAGGCG GGCAGUUUGA CUGGGGCGGU CGCCUCCUAA AAGGUAACGG AGGCGCUCAA AGGUUCCC U CAGAAUGGUU GGAAAUCAUU CAUAGAGUGU AAAGGCAUAA GGGAGCUUGA CUGCGAGACC UACAAGUCGA GCAGGGUCG AAAGACGGAC UUAGUGAUCC GGUGGUUCCG CAUGGAAGGG CCAUCGCUCA ACGGAUAAAA GCUACCCCGG GGAUAACAGG CUUAUCUCC CCCAAGAGUU CACAUCGACG GGGAGGUUUG GCACCUCGAU GUCGGCUCAU CGCAUCCUGG GGCUGUAGUC G GUCCCAAG GGUUGGGCUG UUCGCCCAUU AAAGCGGUAC GCGAGCUGGG UUCAGAACGU CGUGAGACAG UUCGGUCCCU AU CCGUCGU GGGCGUAGGA AAUUUGAGAG GAGCUGUCCU UAGUACGAGA GGACCGGGAU GGACAUACCU CUGGUGUACC AGU UGUCGU GCCAACGGCA UAGCUGGGUA GCUAUGUGUG GACGGGAUAA GUGCUGAAAG CAUCUAAGCA UGAAGCCCCC CUCA AGAUG AGAUUUCCCA ACUUCGGUUA UAAGAUCCCU CAAAGAUGAU GAGGUUAAUA GGUUCGAGGU GGAAGCAUGG UGACA UGUG GAGCUGACGA AUACUAAUCG AUCGAAGACU UAAUCAA

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Macromolecule #9: 5S rRNA

MacromoleculeName: 5S rRNA / type: rna / ID: 9 / Number of copies: 1
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 36.974945 KDa
SequenceString:
UCUGGUGACU AUAGCAAGGA GGUCACACCU GUUCCCAUGC CGAACACAGA AGUUAAGCUC CUUAGCGUCG AUGGUAGUCG AACUUACGU UCCGCUAGAG UAGAACGUUG CCAGGC

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Macromolecule #34: 16S rRNA

MacromoleculeName: 16S rRNA / type: rna / ID: 34 / Number of copies: 1
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 500.724594 KDa
SequenceString: AUGGAGAGUU UGAUCCUGGC UCAGGAUGAA CGCUGGCGGC GUGCCUAAUA CAUGCAAGUC GAGCGAACGG ACGAGAAGCU UGCUUCUCU GAUGUUAGCG GCGGACGGGU GAGUAACACG UGGAUAACCU ACCUAUAAGA CUGGGAUAAC UUCGGGAAAC C GGAGCUAA ...String:
AUGGAGAGUU UGAUCCUGGC UCAGGAUGAA CGCUGGCGGC GUGCCUAAUA CAUGCAAGUC GAGCGAACGG ACGAGAAGCU UGCUUCUCU GAUGUUAGCG GCGGACGGGU GAGUAACACG UGGAUAACCU ACCUAUAAGA CUGGGAUAAC UUCGGGAAAC C GGAGCUAA UACCGGAUAA UAUUUUGAAC CGCAUGGUUC AAAAGUGAAA GACGGUCUUG CUGUCACUUA UAGAUGGAUC CG CGCUGCA UUAGCUAGUU GGUAAGGUAA CGGCUUACCA AGGCAACGAU GCAUAGCCGA CCUGAGAGGG UGAUCGGCCA CAC UGGAAC UGAGACACGG UCCAGACUCC UACGGGAGGC AGCAGUAGGG AAUCUUCCGC AAUGGGCGAA AGCCUGACGG AGCA ACGCC GCGUGAGUGA UGAAGGUCUU CGGAUCGUAA AACUCUGUUA UUAGGGAAGA ACAUAUGUGU AAGUAACUGU GCACA UCUU GACGGUACCU AAUCAGAAAG CCACGGCUAA CUACGUGCCA GCAGCCGCGG UAAUACGUAG GUGGCAAGCG UUAUCC GGA AUUAUUGGGC GUAAAGCGCG CGUAGGCGGU UUUUUAAGUC UGAUGUGAAA GCCCACGGCU CAACCGUGGA GGGUCAU UG GAAACUGGAA AACUUGAGUG CAGAAGAGGA AAGUGGAAUU CCAUGUGUAG CGGUGAAAUG CGCAGAGAUA UGGAGGAA C ACCAGUGGCG AAGGCGACUU UCUGGUCUGU AACUGACGCU GAUGUGCGAA AGCGUGGGGA UCAAACAGGA UUAGAUACC CUGGUAGUCC ACGCCGUAAA CGAUGAGUGC UAAGUGUUAG GGGGUUUCCG CCCCUUAGUG CUGCAGCUAA CGCAUUAAGC ACUCCGCCU GGGGAGUACG ACCGCAAGGU UGAAACUCAA AGGAAUUGAC GGGGACCCGC ACAAGCGGUG GAGCAUGUGG U UUAAUUCG AAGCAACGCG AAGAACCUUA CCAAAUCUUG ACAUCCUUUG ACAACUCUAG AGAUAGAGC(N) UUCCCCUUCG GGGGACAAA GUGACAGGUG GUGCAUGGUU GUCGUCAGCU CGUGUCGUGA GAUGUUGGGU UAAGUCCCGC AACGAGCGCA A CCCUUAAG CUUAGUUGCC AUCAUUAAGU UGGGCACUCU AAGUUGACUG CCGGUGACAA ACCGGAGGAA GGUGGGGAUG AC GUCAAAU CAUCAUGCCC CUUAUGAUUU GGGCUACACA CGUGCUACAA UGGACAAUAC AAAGGGCAGC GAAACCGCGA GGU CAAGCA AAUCCCAUAA AGUUGUUCUC AGUUCGGAUU GUAGUCUGCA ACUCGACUAC AUGAAGCUGG AAUCGCUAGU AAUC GUAGA UCAGCAUGCU ACGGUGAAUA CGUUCCCGGG UCUUGUACAC ACCGCCCGUC ACACCACGAG AGUUUGUAAC ACCCG AAGC CGGUGGAGUA ACCUUUUAGG AGCUAGCCGU CGAAGGUGGG ACAAAUGAUU GGGGUGAAGU CGUAACAAGG UAGCCG UAU CGGAAGGUGC GGCUGGAUCA CCUCCUU

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Macromolecule #54: ZINC ION

MacromoleculeName: ZINC ION / type: ligand / ID: 54 / Number of copies: 3 / Formula: ZN
Molecular weightTheoretical: 65.409 Da

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Macromolecule #55: ADENOSINE-5'-TRIPHOSPHATE

MacromoleculeName: ADENOSINE-5'-TRIPHOSPHATE / type: ligand / ID: 55 / Number of copies: 2 / Formula: ATP
Molecular weightTheoretical: 507.181 Da
Chemical component information

ChemComp-ATP:
ADENOSINE-5'-TRIPHOSPHATE / ATP, energy-carrying molecule*YM

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Macromolecule #56: MAGNESIUM ION

MacromoleculeName: MAGNESIUM ION / type: ligand / ID: 56 / Number of copies: 2 / Formula: MG
Molecular weightTheoretical: 24.305 Da

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 7.5
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Image recordingFilm or detector model: GATAN K2 SUMMIT (4k x 4k) / Detector mode: COUNTING / Average electron dose: 60.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Final reconstructionResolution.type: BY AUTHOR / Resolution: 2.9 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 59889
Initial angle assignmentType: MAXIMUM LIKELIHOOD
Final angle assignmentType: MAXIMUM LIKELIHOOD
FSC plot (resolution estimation)

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