+Open data
-Basic information
Entry | Database: EMDB / ID: EMD-10181 | ||||||||||||||||||
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Title | Rabbit 80S ribosome stalled on a poly(A) tail | ||||||||||||||||||
Map data | Structure of rabbit 80S stalled on a polyA tail | ||||||||||||||||||
Sample |
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Function / homology | Function and homology information regulation of G1 to G0 transition / exit from mitosis / positive regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator / regulation of translation involved in cellular response to UV / protein-DNA complex disassembly / positive regulation of DNA damage response, signal transduction by p53 class mediator resulting in transcription of p21 class mediator / optic nerve development / retinal ganglion cell axon guidance / mammalian oogenesis stage / G1 to G0 transition ...regulation of G1 to G0 transition / exit from mitosis / positive regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator / regulation of translation involved in cellular response to UV / protein-DNA complex disassembly / positive regulation of DNA damage response, signal transduction by p53 class mediator resulting in transcription of p21 class mediator / optic nerve development / retinal ganglion cell axon guidance / mammalian oogenesis stage / G1 to G0 transition / activation-induced cell death of T cells / positive regulation of signal transduction by p53 class mediator / ubiquitin ligase inhibitor activity / phagocytic cup / 90S preribosome / TOR signaling / T cell proliferation involved in immune response / protein-RNA complex assembly / erythrocyte development / cellular response to actinomycin D / negative regulation of ubiquitin-dependent protein catabolic process / ribosomal small subunit export from nucleus / translation regulator activity / rough endoplasmic reticulum / gastrulation / MDM2/MDM4 family protein binding / maturation of LSU-rRNA / DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest / cytosolic ribosome / maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / class I DNA-(apurinic or apyrimidinic site) endonuclease activity / DNA-(apurinic or apyrimidinic site) lyase / rescue of stalled ribosome / ribosomal large subunit biogenesis / maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / maturation of SSU-rRNA / cellular response to leukemia inhibitory factor / positive regulation of translation / small-subunit processome / protein kinase C binding / positive regulation of apoptotic signaling pathway / positive regulation of protein-containing complex assembly / placenta development / cellular response to gamma radiation / mRNA 5'-UTR binding / transcription coactivator binding / spindle / cytoplasmic ribonucleoprotein granule / modification-dependent protein catabolic process / G1/S transition of mitotic cell cycle / protein tag activity / rRNA processing / ribosomal small subunit biogenesis / antimicrobial humoral immune response mediated by antimicrobial peptide / rhythmic process / positive regulation of canonical Wnt signaling pathway / small ribosomal subunit rRNA binding / ribosome binding / glucose homeostasis / regulation of translation / ribosomal small subunit assembly / retina development in camera-type eye / small ribosomal subunit / 5S rRNA binding / large ribosomal subunit rRNA binding / T cell differentiation in thymus / cytosolic small ribosomal subunit / ribosomal large subunit assembly / cell body / cytoplasmic translation / perikaryon / cytosolic large ribosomal subunit / defense response to Gram-negative bacterium / killing of cells of another organism / tRNA binding / mitochondrial inner membrane / postsynaptic density / cell differentiation / protein stabilization / rRNA binding / ribosome / protein ubiquitination / structural constituent of ribosome / positive regulation of apoptotic process / positive regulation of protein phosphorylation / ribonucleoprotein complex / translation / cell division / DNA repair / mRNA binding / centrosome / dendrite / ubiquitin protein ligase binding / positive regulation of cell population proliferation / synapse / positive regulation of gene expression / negative regulation of apoptotic process / nucleolus / apoptotic process / protein kinase binding Similarity search - Function | ||||||||||||||||||
Biological species | Oryctolagus cuniculus (rabbit) / Homo sapiens (human) / Rabbit (rabbit) | ||||||||||||||||||
Method | single particle reconstruction / cryo EM / Resolution: 2.8 Å | ||||||||||||||||||
Authors | Chandrasekaran V / Juszkiewicz S / Choi J / Puglisi JD / Brown A / Shao S / Ramakrishnan V / Hegde RS | ||||||||||||||||||
Funding support | United Kingdom, United States, 5 items
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Citation | Journal: Nat Struct Mol Biol / Year: 2019 Title: Mechanism of ribosome stalling during translation of a poly(A) tail. Authors: Viswanathan Chandrasekaran / Szymon Juszkiewicz / Junhong Choi / Joseph D Puglisi / Alan Brown / Sichen Shao / V Ramakrishnan / Ramanujan S Hegde / Abstract: Faulty or damaged messenger RNAs are detected by the cell when translating ribosomes stall during elongation and trigger pathways of mRNA decay, nascent protein degradation and ribosome recycling. ...Faulty or damaged messenger RNAs are detected by the cell when translating ribosomes stall during elongation and trigger pathways of mRNA decay, nascent protein degradation and ribosome recycling. The most common mRNA defect in eukaryotes is probably inappropriate polyadenylation at near-cognate sites within the coding region. How ribosomes stall selectively when they encounter poly(A) is unclear. Here, we use biochemical and structural approaches in mammalian systems to show that poly-lysine, encoded by poly(A), favors a peptidyl-transfer RNA conformation suboptimal for peptide bond formation. This conformation partially slows elongation, permitting poly(A) mRNA in the ribosome's decoding center to adopt a ribosomal RNA-stabilized single-stranded helix. The reconfigured decoding center clashes with incoming aminoacyl-tRNA, thereby precluding elongation. Thus, coincidence detection of poly-lysine in the exit tunnel and poly(A) in the decoding center allows ribosomes to detect aberrant mRNAs selectively, stall elongation and trigger downstream quality control pathways essential for cellular homeostasis. | ||||||||||||||||||
History |
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-Structure visualization
Movie |
Movie viewer |
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Structure viewer | EM map: SurfViewMolmilJmol/JSmol |
Supplemental images |
-Downloads & links
-EMDB archive
Map data | emd_10181.map.gz | 63.5 MB | EMDB map data format | |
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Header (meta data) | emd-10181-v30.xml emd-10181.xml | 112.4 KB 112.4 KB | Display Display | EMDB header |
FSC (resolution estimation) | emd_10181_fsc.xml | 18 KB | Display | FSC data file |
Images | emd_10181.png | 271.8 KB | ||
Masks | emd_10181_msk_1.map | 512 MB | Mask map | |
Others | emd_10181_additional.map.gz emd_10181_half_map_1.map.gz emd_10181_half_map_2.map.gz | 411.2 MB 413.5 MB 413.5 MB | ||
Archive directory | http://ftp.pdbj.org/pub/emdb/structures/EMD-10181 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-10181 | HTTPS FTP |
-Validation report
Summary document | emd_10181_validation.pdf.gz | 486.8 KB | Display | EMDB validaton report |
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Full document | emd_10181_full_validation.pdf.gz | 486.4 KB | Display | |
Data in XML | emd_10181_validation.xml.gz | 26.2 KB | Display | |
Data in CIF | emd_10181_validation.cif.gz | 34.6 KB | Display | |
Arichive directory | https://ftp.pdbj.org/pub/emdb/validation_reports/EMD-10181 ftp://ftp.pdbj.org/pub/emdb/validation_reports/EMD-10181 | HTTPS FTP |
-Related structure data
Related structure data | 6sgcMC M: atomic model generated by this map C: citing same article (ref.) |
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Similar structure data |
-Links
EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
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Related items in Molecule of the Month |
-Map
File | Download / File: emd_10181.map.gz / Format: CCP4 / Size: 512 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Annotation | Structure of rabbit 80S stalled on a polyA tail | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Projections & slices | Image control
Images are generated by Spider. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 1.085 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
CCP4 map header:
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-Supplemental data
-Mask #1
File | emd_10181_msk_1.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Additional map: Unsharpened map
File | emd_10181_additional.map | ||||||||||||
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Annotation | Unsharpened map | ||||||||||||
Projections & Slices |
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Density Histograms |
-Half map: Half1 map
File | emd_10181_half_map_1.map | ||||||||||||
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Annotation | Half1 map | ||||||||||||
Projections & Slices |
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Density Histograms |
-Half map: Half2 map
File | emd_10181_half_map_2.map | ||||||||||||
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Annotation | Half2 map | ||||||||||||
Projections & Slices |
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Density Histograms |
-Sample components
+Entire : Rabbit 80S ribosome stalled on polyA mRNA
+Supramolecule #1: Rabbit 80S ribosome stalled on polyA mRNA
+Supramolecule #2: ribosome
+Supramolecule #3: polyA mRNA
+Macromolecule #1: 18S ribosomal RNA
+Macromolecule #35: polyA mRNA
+Macromolecule #80: 28S ribosomal RNA
+Macromolecule #81: 5S ribosomal RNA
+Macromolecule #82: 5.8S ribosomal RNA
+Macromolecule #85: tRNA (Lys3)
+Macromolecule #2: uS2
+Macromolecule #3: 40S ribosomal protein S3a
+Macromolecule #4: uS5
+Macromolecule #5: uS5
+Macromolecule #6: 40S ribosomal protein S4
+Macromolecule #7: Ribosomal protein S5
+Macromolecule #8: 40S ribosomal protein S6
+Macromolecule #9: 40S ribosomal protein S7
+Macromolecule #10: 40S ribosomal protein S8
+Macromolecule #11: Ribosomal protein S9 (Predicted)
+Macromolecule #12: S10_plectin domain-ontaining protein
+Macromolecule #13: Ribosomal protein S11
+Macromolecule #14: 40S ribosomal protein S12
+Macromolecule #15: Ribosomal_S13_N domain-containing protein
+Macromolecule #16: uS11
+Macromolecule #17: uS19
+Macromolecule #18: Ribosomal protein S16
+Macromolecule #19: eS17
+Macromolecule #20: eS13
+Macromolecule #21: eS19
+Macromolecule #22: Ribosomal_S10 domain-containing protein
+Macromolecule #23: eS21
+Macromolecule #24: Ribosomal protein S15a
+Macromolecule #25: uS12
+Macromolecule #26: eS24
+Macromolecule #27: eS25
+Macromolecule #28: eS26
+Macromolecule #29: 40S ribosomal protein S27
+Macromolecule #30: Ribosomal protein S28
+Macromolecule #31: uS14
+Macromolecule #32: 40S ribosomal protein S30
+Macromolecule #33: Ribosomal protein S27a
+Macromolecule #34: WD_REPEATS_REGION domain-containing protein
+Macromolecule #36: Ribosomal protein L8
+Macromolecule #37: uL3
+Macromolecule #38: uL4
+Macromolecule #39: 60S ribosomal protein L5
+Macromolecule #40: 60S ribosomal protein L6
+Macromolecule #41: uL30
+Macromolecule #42: eL8
+Macromolecule #43: uL6
+Macromolecule #44: 60S ribosomal protein L10
+Macromolecule #45: Ribosomal protein L11
+Macromolecule #46: eL13
+Macromolecule #47: Ribosomal protein L14
+Macromolecule #48: Ribosomal protein L15
+Macromolecule #49: uL13
+Macromolecule #50: uL22
+Macromolecule #51: eL18
+Macromolecule #52: eL19
+Macromolecule #53: eL20
+Macromolecule #54: eL21
+Macromolecule #55: eL22
+Macromolecule #56: Ribosomal protein L23
+Macromolecule #57: TRASH domain-containing protein
+Macromolecule #58: Ribosomal_L23eN domain-containing protein
+Macromolecule #59: Ribosomal protein L26
+Macromolecule #60: 60S ribosomal protein L27
+Macromolecule #61: Ribosomal_L18e/L15P domain-containing protein
+Macromolecule #62: eL29
+Macromolecule #63: Ribosomal_L7Ae domain-containing protein
+Macromolecule #64: eL31
+Macromolecule #65: eL32
+Macromolecule #66: eL33
+Macromolecule #67: eL34
+Macromolecule #68: uL29
+Macromolecule #69: 60S ribosomal protein L36
+Macromolecule #70: Ribosomal protein L37
+Macromolecule #71: eL38
+Macromolecule #72: eL39
+Macromolecule #73: eL40
+Macromolecule #74: 60s ribosomal protein l41
+Macromolecule #75: eL42
+Macromolecule #76: eL43
+Macromolecule #77: Ribosomal_L28e domain-containing protein
+Macromolecule #78: uL10
+Macromolecule #79: Ribosomal protein L12
+Macromolecule #83: poly-lysine nascent chain
+Macromolecule #84: Ribosomal protein
+Macromolecule #86: MAGNESIUM ION
+Macromolecule #87: ZINC ION
+Macromolecule #88: SPERMIDINE
-Experimental details
-Structure determination
Method | cryo EM |
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Processing | single particle reconstruction |
Aggregation state | particle |
-Sample preparation
Buffer | pH: 7.4 |
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Vitrification | Cryogen name: ETHANE |
-Electron microscopy
Microscope | FEI TITAN KRIOS |
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Image recording | #0 - Image recording ID: 1 / #0 - Film or detector model: FEI FALCON III (4k x 4k) / #0 - Detector mode: INTEGRATING / #0 - Average electron dose: 41.8 e/Å2 / #1 - Image recording ID: 2 / #1 - Film or detector model: FEI FALCON III (4k x 4k) / #1 - Average electron dose: 41.8 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD |
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |
+Image processing
-Atomic model buiding 1
Refinement | Space: REAL / Protocol: FLEXIBLE FIT |
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Output model | PDB-6sgc: |