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- PDB-8oop: CryoEM Structure INO80core Hexasome complex composite model state2 -

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基本情報

登録情報
データベース: PDB / ID: 8oop
タイトルCryoEM Structure INO80core Hexasome complex composite model state2
要素
  • (Chromatin-remodeling ...) x 2
  • (RuvB-like protein ...) x 2
  • Actin-related protein 5
  • DNA Strand 2デオキシリボ核酸
  • DNA strand 1デオキシリボ核酸
  • Histone H2AヒストンH2A
  • Histone H2BヒストンH2B
  • Histone H3.1ヒストンH3
  • Histone H4ヒストンH4
  • Ino eighty subunit 2
キーワードDNA BINDING PROTEIN (DNA結合タンパク質) / ATP-dependent chromatin remodeler
機能・相同性
機能・相同性情報


DASH complex / protein transport along microtubule to mitotic spindle pole body / mitotic sister chromatid biorientation / attachment of spindle microtubules to kinetochore / Ino80 complex / attachment of mitotic spindle microtubules to kinetochore / negative regulation of megakaryocyte differentiation / protein localization to CENP-A containing chromatin / ATP-dependent activity, acting on DNA / Chromatin modifying enzymes ...DASH complex / protein transport along microtubule to mitotic spindle pole body / mitotic sister chromatid biorientation / attachment of spindle microtubules to kinetochore / Ino80 complex / attachment of mitotic spindle microtubules to kinetochore / negative regulation of megakaryocyte differentiation / protein localization to CENP-A containing chromatin / ATP-dependent activity, acting on DNA / Chromatin modifying enzymes / Replacement of protamines by nucleosomes in the male pronucleus / CENP-A containing nucleosome / epigenetic regulation of gene expression / Packaging Of Telomere Ends / Recognition and association of DNA glycosylase with site containing an affected purine / Cleavage of the damaged purine / Deposition of new CENPA-containing nucleosomes at the centromere / Recognition and association of DNA glycosylase with site containing an affected pyrimidine / Cleavage of the damaged pyrimidine / Inhibition of DNA recombination at telomere / Meiotic synapsis / telomere organization / RNA Polymerase I Promoter Opening / Interleukin-7 signaling / Assembly of the ORC complex at the origin of replication / SUMOylation of chromatin organization proteins / DNAメチル化 / Condensation of Prophase Chromosomes / ERCC6 (CSB) and EHMT2 (G9a) positively regulate rRNA expression / Chromatin modifications during the maternal to zygotic transition (MZT) / SIRT1 negatively regulates rRNA expression / HCMV Late Events / innate immune response in mucosa / PRC2 methylates histones and DNA / Defective pyroptosis / helicase activity / HDACs deacetylate histones / RNA Polymerase I Promoter Escape / Nonhomologous End-Joining (NHEJ) / Transcriptional regulation by small RNAs / Formation of the beta-catenin:TCF transactivating complex / RUNX1 regulates genes involved in megakaryocyte differentiation and platelet function / Activated PKN1 stimulates transcription of AR (androgen receptor) regulated genes KLK2 and KLK3 / NoRC negatively regulates rRNA expression / B-WICH complex positively regulates rRNA expression / G2/M DNA damage checkpoint / HDMs demethylate histones / DNA Damage/Telomere Stress Induced Senescence / 紡錘体 / 動原体 / Metalloprotease DUBs / PKMTs methylate histone lysines / RMTs methylate histone arginines / 遺伝的組換え / Pre-NOTCH Transcription and Translation / nucleosome assembly / Activation of anterior HOX genes in hindbrain development during early embryogenesis / HCMV Early Events / Transcriptional regulation of granulopoiesis / structural constituent of chromatin / UCH proteinases / ヌクレオソーム / antimicrobial humoral immune response mediated by antimicrobial peptide / E3 ubiquitin ligases ubiquitinate target proteins / Recruitment and ATM-mediated phosphorylation of repair and signaling proteins at DNA double strand breaks / RUNX1 regulates transcription of genes involved in differentiation of HSCs / 遺伝子発現 / chromatin organization / Factors involved in megakaryocyte development and platelet production / Processing of DNA double-strand break ends / HATs acetylate histones / antibacterial humoral response / Senescence-Associated Secretory Phenotype (SASP) / Oxidative Stress Induced Senescence / ヘリカーゼ / Estrogen-dependent gene expression / chromosome, telomeric region / Ub-specific processing proteases / defense response to Gram-positive bacterium / クロマチンリモデリング / cadherin binding / protein heterodimerization activity / Amyloid fiber formation / negative regulation of cell population proliferation / DNA修復 / ATP hydrolysis activity / protein-containing complex / DNA binding / extracellular space / RNA binding / extracellular exosome / extracellular region / 核質 / ATP binding / 生体膜 / identical protein binding / 細胞核 / 細胞質基質
類似検索 - 分子機能
DASH complex subunit Dad4 / DASH complex subunit Dad4 / INO80 complex subunit B-like conserved region / INO80 complex, subunit Ies2 / INO80 complex, subunit Ies6 / PAPA-1-like conserved region / PAPA-1 / Vps72/YL1, C-terminal / YL1 nuclear protein C-terminal domain / YL1 nuclear protein C-terminal domain ...DASH complex subunit Dad4 / DASH complex subunit Dad4 / INO80 complex subunit B-like conserved region / INO80 complex, subunit Ies2 / INO80 complex, subunit Ies6 / PAPA-1-like conserved region / PAPA-1 / Vps72/YL1, C-terminal / YL1 nuclear protein C-terminal domain / YL1 nuclear protein C-terminal domain / RuvB-like / RuvB-like, AAA-lid domain / RuvBL1/2, DNA/RNA binding domain / TIP49 P-loop domain / TIP49 AAA-lid domain / TIP49, P-loop domain / アクチン / Actin family / アクチン / Histone H2B signature. / ヒストンH2B / ヒストンH2B / Histone H2A conserved site / Histone H2A signature. / Histone H2A, C-terminal domain / C-terminus of histone H2A / ヒストンH2A / Histone 2A / Histone H4, conserved site / Histone H4 signature. / ヒストンH4 / ヒストンH4 / CENP-T/Histone H4, histone fold / Centromere kinetochore component CENP-T histone fold / TATA box binding protein associated factor / TATA box binding protein associated factor (TAF), histone-like fold domain / Histone H3 signature 1. / Histone H3 signature 2. / ヒストンH3 / Histone H3/CENP-A / Histone H2A/H2B/H3 / Core histone H2A/H2B/H3/H4 / ATPase, nucleotide binding domain / Histone-fold / ATPases associated with a variety of cellular activities / AAA+ ATPase domain / P-loop containing nucleoside triphosphate hydrolase
類似検索 - ドメイン・相同性
ADENOSINE-5'-DIPHOSPHATE / TETRAFLUOROALUMINATE ION / ADENOSINE-5'-TRIPHOSPHATE / デオキシリボ核酸 / DNA (> 10) / DNA (> 100) / INO80 complex subunit B-like conserved region domain-containing protein / RuvB-like helicase / RuvB-like helicase / Uncharacterized protein ...ADENOSINE-5'-DIPHOSPHATE / TETRAFLUOROALUMINATE ION / ADENOSINE-5'-TRIPHOSPHATE / デオキシリボ核酸 / DNA (> 10) / DNA (> 100) / INO80 complex subunit B-like conserved region domain-containing protein / RuvB-like helicase / RuvB-like helicase / Uncharacterized protein / Vps72/YL1 C-terminal domain-containing protein / ヒストンH4 / Histone H2B type 1-C/E/F/G/I / ヒストンH3 / Histone H2A type 1-C
類似検索 - 構成要素
生物種Thermochaetoides thermophila (菌類)
Homo sapiens (ヒト)
synthetic construct (人工物)
手法電子顕微鏡法 / 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 2.7 Å
データ登録者Zhang, M. / Jungblut, A. / Hoffmann, T. / Eustermann, S.
資金援助 ドイツ, European Union, 4件
組織認可番号国
EIPOD fellowship under Marie Sklodowska-Curie Actions COFUND847543 ドイツ
European Research Council (ERC)833613European Union
German Research Foundation (DFG)CRC136 ドイツ
German Research Foundation (DFG)CRC1064 ドイツ
引用
ジャーナル: Science / 年: 2023
タイトル: Hexasome-INO80 complex reveals structural basis of noncanonical nucleosome remodeling.
著者: Min Zhang / Anna Jungblut / Franziska Kunert / Luis Hauptmann / Thomas Hoffmann / Olga Kolesnikova / Felix Metzner / Manuela Moldt / Felix Weis / Frank DiMaio / Karl-Peter Hopfner / Sebastian Eustermann /
要旨: Loss of H2A-H2B histone dimers is a hallmark of actively transcribed genes, but how the cellular machinery functions in the context of noncanonical nucleosomal particles remains largely elusive. In ...Loss of H2A-H2B histone dimers is a hallmark of actively transcribed genes, but how the cellular machinery functions in the context of noncanonical nucleosomal particles remains largely elusive. In this work, we report the structural mechanism for adenosine 5'-triphosphate-dependent chromatin remodeling of hexasomes by the INO80 complex. We show how INO80 recognizes noncanonical DNA and histone features of hexasomes that emerge from the loss of H2A-H2B. A large structural rearrangement switches the catalytic core of INO80 into a distinct, spin-rotated mode of remodeling while its nuclear actin module remains tethered to long stretches of unwrapped linker DNA. Direct sensing of an exposed H3-H4 histone interface activates INO80, independently of the H2A-H2B acidic patch. Our findings reveal how the loss of H2A-H2B grants remodelers access to a different, yet unexplored layer of energy-driven chromatin regulation.
#1: ジャーナル: Acta Crystallogr D Struct Biol / 年: 2018
タイトル: Real-space refinement in PHENIX for cryo-EM and crystallography.
著者: Pavel V Afonine / Billy K Poon / Randy J Read / Oleg V Sobolev / Thomas C Terwilliger / Alexandre Urzhumtsev / Paul D Adams /
要旨: This article describes the implementation of real-space refinement in the phenix.real_space_refine program from the PHENIX suite. The use of a simplified refinement target function enables very fast ...This article describes the implementation of real-space refinement in the phenix.real_space_refine program from the PHENIX suite. The use of a simplified refinement target function enables very fast calculation, which in turn makes it possible to identify optimal data-restraint weights as part of routine refinements with little runtime cost. Refinement of atomic models against low-resolution data benefits from the inclusion of as much additional information as is available. In addition to standard restraints on covalent geometry, phenix.real_space_refine makes use of extra information such as secondary-structure and rotamer-specific restraints, as well as restraints or constraints on internal molecular symmetry. The re-refinement of 385 cryo-EM-derived models available in the Protein Data Bank at resolutions of 6 Å or better shows significant improvement of the models and of the fit of these models to the target maps.
#2: ジャーナル: Biochem J / 年: 2021
タイトル: New tools for automated cryo-EM single-particle analysis in RELION-4.0.
著者: Dari Kimanius / Liyi Dong / Grigory Sharov / Takanori Nakane / Sjors H W Scheres /
要旨: We describe new tools for the processing of electron cryo-microscopy (cryo-EM) images in the fourth major release of the RELION software. In particular, we introduce VDAM, a variable-metric gradient ...We describe new tools for the processing of electron cryo-microscopy (cryo-EM) images in the fourth major release of the RELION software. In particular, we introduce VDAM, a variable-metric gradient descent algorithm with adaptive moments estimation, for image refinement; a convolutional neural network for unsupervised selection of 2D classes; and a flexible framework for the design and execution of multiple jobs in pre-defined workflows. In addition, we present a stand-alone utility called MDCatch that links the execution of jobs within this framework with metadata gathering during microscope data acquisition. The new tools are aimed at providing fast and robust procedures for unsupervised cryo-EM structure determination, with potential applications for on-the-fly processing and the development of flexible, high-throughput structure determination pipelines. We illustrate their potential on 12 publicly available cryo-EM data sets.
#3: ジャーナル: Acta Crystallogr D Struct Biol / 年: 2018
タイトル: ISOLDE: a physically realistic environment for model building into low-resolution electron-density maps.
著者: Tristan Ian Croll /
要旨: This paper introduces ISOLDE, a new software package designed to provide an intuitive environment for high-fidelity interactive remodelling/refinement of macromolecular models into electron-density ...This paper introduces ISOLDE, a new software package designed to provide an intuitive environment for high-fidelity interactive remodelling/refinement of macromolecular models into electron-density maps. ISOLDE combines interactive molecular-dynamics flexible fitting with modern molecular-graphics visualization and established structural biology libraries to provide an immersive interface wherein the model constantly acts to maintain physically realistic conformations as the user interacts with it by directly tugging atoms with a mouse or haptic interface or applying/removing restraints. In addition, common validation tasks are accelerated and visualized in real time. Using the recently described 3.8 Å resolution cryo-EM structure of the eukaryotic minichromosome maintenance (MCM) helicase complex as a case study, it is demonstrated how ISOLDE can be used alongside other modern refinement tools to avoid common pitfalls of low-resolution modelling and improve the quality of the final model. A detailed analysis of changes between the initial and final model provides a somewhat sobering insight into the dangers of relying on a small number of validation metrics to judge the quality of a low-resolution model.
#4: ジャーナル: Acta Crystallogr D Biol Crystallogr / 年: 2010
タイトル: Features and development of Coot.
著者: P Emsley / B Lohkamp / W G Scott / K Cowtan /
要旨: Coot is a molecular-graphics application for model building and validation of biological macromolecules. The program displays electron-density maps and atomic models and allows model manipulations ...Coot is a molecular-graphics application for model building and validation of biological macromolecules. The program displays electron-density maps and atomic models and allows model manipulations such as idealization, real-space refinement, manual rotation/translation, rigid-body fitting, ligand search, solvation, mutations, rotamers and Ramachandran idealization. Furthermore, tools are provided for model validation as well as interfaces to external programs for refinement, validation and graphics. The software is designed to be easy to learn for novice users, which is achieved by ensuring that tools for common tasks are 'discoverable' through familiar user-interface elements (menus and toolbars) or by intuitive behaviour (mouse controls). Recent developments have focused on providing tools for expert users, with customisable key bindings, extensions and an extensive scripting interface. The software is under rapid development, but has already achieved very widespread use within the crystallographic community. The current state of the software is presented, with a description of the facilities available and of some of the underlying methods employed.
履歴
登録2023年4月5日登録サイト: PDBE / 処理サイト: PDBE
改定 1.02023年7月26日Provider: repository / タイプ: Initial release
改定 1.12023年8月2日Group: Database references / カテゴリ: citation / citation_author
Item: _citation.journal_volume / _citation.page_first ..._citation.journal_volume / _citation.page_first / _citation.page_last / _citation_author.identifier_ORCID

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構造の表示

構造ビューア分子:
MolmilJmol/JSmol

ダウンロードとリンク

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集合体

登録構造単位
A: RuvB-like protein 1
B: RuvB-like protein 1
C: RuvB-like protein 1
D: RuvB-like protein 2
E: RuvB-like protein 2
F: RuvB-like protein 2
G: Chromatin-remodeling ATPase Ino80
H: Ino eighty subunit 2
I: Chromatin-remodeling complex subunit IES6
J: Actin-related protein 5
K: DNA strand 1
L: DNA Strand 2
M: Histone H3.1
N: Histone H4
O: Histone H2A
P: Histone H2B
Q: Histone H3.1
R: Histone H4
ヘテロ分子


分子量 (理論値)分子数
合計 (水以外)830,29729
ポリマ－826,64818
非ポリマー3,64911
水0
1


  • 登録構造と同一
  • 登録者・ソフトウェアが定義した集合体
  • 根拠: mass spectrometry
タイプ名称対称操作数
identity operation1_555x,y,z1

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要素

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RuvB-like protein ... , 2種, 6分子 ABCDEF

#1: タンパク質 RuvB-like protein 1 / Rvb1


分子量: 50451.848 Da / 分子数: 3 / 由来タイプ: 組換発現
由来: (組換発現) Thermochaetoides thermophila (菌類)
遺伝子: CTHT_0006820 / 発現宿主: Trichoplusia ni (イラクサキンウワバ) / 参照: UniProt: G0RYI5, ヘリカーゼ
#2: タンパク質 RuvB-like protein 2 / Rvb2


分子量: 53212.746 Da / 分子数: 3 / 由来タイプ: 組換発現
由来: (組換発現) Thermochaetoides thermophila (菌類)
遺伝子: CTHT_0006170 / 発現宿主: Trichoplusia ni (イラクサキンウワバ) / 参照: UniProt: G0RYC2, ヘリカーゼ

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Chromatin-remodeling ... , 2種, 2分子 GI

#3: タンパク質 Chromatin-remodeling ATPase Ino80


分子量: 130887.656 Da / 分子数: 1 / 由来タイプ: 組換発現
由来: (組換発現) Thermochaetoides thermophila (菌類)
発現宿主: Trichoplusia ni (イラクサキンウワバ)
#5: タンパク質 Chromatin-remodeling complex subunit IES6


分子量: 23127.523 Da / 分子数: 1 / 由来タイプ: 組換発現
由来: (組換発現) Thermochaetoides thermophila (菌類)
遺伝子: CTHT_0032670 / 発現宿主: Trichoplusia ni (イラクサキンウワバ) / 参照: UniProt: G0S590

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タンパク質 , 6種, 8分子 HJMQNROP

#4: タンパク質 Ino eighty subunit 2


分子量: 53345.980 Da / 分子数: 1 / 由来タイプ: 組換発現
由来: (組換発現) Thermochaetoides thermophila (菌類)
遺伝子: CTHT_0004910 / 発現宿主: Trichoplusia ni (イラクサキンウワバ) / 参照: UniProt: G0RY01
#6: タンパク質 Actin-related protein 5 / Outer kinetochore protein DAD4


分子量: 87773.086 Da / 分子数: 1 / 由来タイプ: 組換発現
由来: (組換発現) Thermochaetoides thermophila (菌類)
遺伝子: CTHT_0032660 / 発現宿主: Trichoplusia ni (イラクサキンウワバ) / 参照: UniProt: G0S589
#9: タンパク質 Histone H3.1 / ヒストンH3 / Histone H3/a / Histone H3/b / Histone H3/c / Histone H3/d / Histone H3/f / Histone H3/h / Histone ...Histone H3/a / Histone H3/b / Histone H3/c / Histone H3/d / Histone H3/f / Histone H3/h / Histone H3/i / Histone H3/j / Histone H3/k / Histone H3/l


分子量: 15305.969 Da / 分子数: 2 / 由来タイプ: 組換発現 / 由来: (組換発現) Homo sapiens (ヒト)
遺伝子: H3C1, H3FA, HIST1H3A, H3C2, H3FL, HIST1H3B, H3C3, H3FC HIST1H3C, H3C4, H3FB, HIST1H3D, H3C6, H3FD, HIST1H3E, H3C7, H3FI, HIST1H3F, H3C8, H3FH, HIST1H3G, H3C10, H3FK, HIST1H3H, H3C11, H3FF, ...遺伝子: H3C1, H3FA, HIST1H3A, H3C2, H3FL, HIST1H3B, H3C3, H3FC HIST1H3C, H3C4, H3FB, HIST1H3D, H3C6, H3FD, HIST1H3E, H3C7, H3FI, HIST1H3F, H3C8, H3FH, HIST1H3G, H3C10, H3FK, HIST1H3H, H3C11, H3FF, HIST1H3I, H3C12, H3FJ, HIST1H3J
発現宿主: Escherichia coli (大腸菌) / 参照: UniProt: P68431
#10: タンパク質 Histone H4 / ヒストンH4


分子量: 11263.231 Da / 分子数: 2 / 由来タイプ: 組換発現 / 由来: (組換発現) Homo sapiens (ヒト)
遺伝子: HIST1H4A, H4/A, H4FA, HIST1H4B, H4/I, H4FI, HIST1H4C, H4/G, H4FG, HIST1H4D, H4/B, H4FB, HIST1H4E, H4/J, H4FJ, HIST1H4F, H4/C, H4FC, HIST1H4H, H4/H, H4FH, HIST1H4I, H4/M, H4FM, HIST1H4J, ...遺伝子: HIST1H4A, H4/A, H4FA, HIST1H4B, H4/I, H4FI, HIST1H4C, H4/G, H4FG, HIST1H4D, H4/B, H4FB, HIST1H4E, H4/J, H4FJ, HIST1H4F, H4/C, H4FC, HIST1H4H, H4/H, H4FH, HIST1H4I, H4/M, H4FM, HIST1H4J, H4/E, H4FE, HIST1H4K, H4/D, H4FD, HIST1H4L, H4/K, H4FK, HIST2H4A, H4/N, H4F2, H4FN, HIST2H4, HIST2H4B, H4/O, H4FO, HIST4H4
発現宿主: Escherichia coli (大腸菌) / 参照: UniProt: P62805
#11: タンパク質 Histone H2A / ヒストンH2A / H2A-clustered histone 6 / Histone H2A/l


分子量: 14004.329 Da / 分子数: 1 / 由来タイプ: 組換発現 / 由来: (組換発現) Homo sapiens (ヒト) / 遺伝子: H2AC6, H2AFL, HIST1H2AC / 発現宿主: Escherichia coli (大腸菌) / 参照: UniProt: Q93077
#12: タンパク質 Histone H2B / ヒストンH2B / Histone H2B.1 A / Histone H2B.a / H2B/a / Histone H2B.g / H2B/g / Histone H2B.h / H2B/h / Histone ...Histone H2B.1 A / Histone H2B.a / H2B/a / Histone H2B.g / H2B/g / Histone H2B.h / H2B/h / Histone H2B.k / H2B/k / Histone H2B.l / H2B/l


分子量: 13806.018 Da / 分子数: 1 / 由来タイプ: 組換発現 / 由来: (組換発現) Homo sapiens (ヒト)
遺伝子: HIST1H2BC, H2BFL, HIST1H2BE, H2BFH, HIST1H2BF, H2BFG, HIST1H2BG, H2BFA, HIST1H2BI, H2BFK
発現宿主: Escherichia coli (大腸菌) / 参照: UniProt: P62807

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DNA鎖 , 2種, 2分子 KL

#7: DNA鎖 DNA strand 1 / デオキシリボ核酸


分子量: 69527.195 Da / 分子数: 1 / 由来タイプ: 合成 / 由来: (合成) synthetic construct (人工物)
#8: DNA鎖 DNA Strand 2 / デオキシリボ核酸


分子量: 70043.562 Da / 分子数: 1 / 由来タイプ: 合成 / 由来: (合成) synthetic construct (人工物)

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非ポリマー , 4種, 11分子

#13: 化合物
ChemComp-ADP / ADENOSINE-5'-DIPHOSPHATE / ADP / アデノシン二リン酸


分子量: 427.201 Da / 分子数: 7 / 由来タイプ: 合成 / 式: C10H15N5O10P2 / タイプ: SUBJECT OF INVESTIGATION / コメント: ADP, エネルギー貯蔵分子*YM
#14: 化合物 ChemComp-MG / MAGNESIUM ION / マグネシウムジカチオン


分子量: 24.305 Da / 分子数: 2 / 由来タイプ: 合成 / 式: Mg / タイプ: SUBJECT OF INVESTIGATION
#15: 化合物 ChemComp-ALF / TETRAFLUOROALUMINATE ION / テトラフルオロアルミナ-ト


分子量: 102.975 Da / 分子数: 1 / 由来タイプ: 合成 / 式: AlF4 / タイプ: SUBJECT OF INVESTIGATION
#16: 化合物 ChemComp-ATP / ADENOSINE-5'-TRIPHOSPHATE / ATP / アデノシン三リン酸


分子量: 507.181 Da / 分子数: 1 / 由来タイプ: 合成 / 式: C10H16N5O13P3 / タイプ: SUBJECT OF INVESTIGATION / コメント: ATP, エネルギー貯蔵分子*YM

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詳細

研究の焦点であるリガンドがあるかY

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実験情報

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実験

実験手法: 電子顕微鏡法
EM実験試料の集合状態: PARTICLE / ３次元再構成法: 単粒子再構成法

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試料調製

構成要素
ID名称タイプ詳細Entity IDParent-ID由来
1INO80 core module in complex with hexasomeCOMPLEX11-subunit ct INO80 contains two modules (core and Arp8 module) Each module was picked and analyzed separately The core module + hexasome has an overall weight of 0.861MDa The 11-subunit ct INO80 + hexasome has an overall weight of 1.1MDa Ino80, Ies2, Ies6, Ies4,Arp6, Rvb1, Rvb2, Arp8, Arp4, Actin, Taf14 Hexasome DNA, 2xH3, 2xH4, H2A, H2B#1-#120MULTIPLE SOURCES
2Chromatin remodeler INO80COMPLEX#1-#61RECOMBINANT
3HistonesヒストンCOMPLEX#9-#121RECOMBINANT
4DNAデオキシリボ核酸COMPLEX#7-#81RECOMBINANT
分子量値: 0.861 MDa / 実験値: NO
由来(天然)
IDEntity assembly-ID生物種Ncbi tax-ID
22Thermochaetoides thermophila (菌類)209285
33Homo sapiens (ヒト)9606
44Synthetic construct (人工物)32630
由来(組換発現)
IDEntity assembly-ID生物種Ncbi tax-ID
22Trichoplusia ni (イラクサキンウワバ)7111
33Escherichia coli (大腸菌)562
44Synthetic construct (人工物)32630
緩衝液pH: 7.5
詳細: 30mM HEPES, pH7.5 50mM NaCl 0.25mM CaCl2 0.25mM DTT 2mM ADP 3.3mM MgCl2 10mM NaF 2mM AlCl3 0.05% octyl-beta-glucoside
試料濃度: 0.88 mg/ml / 包埋: NO / シャドウイング: NO / 染色: NO / 凍結: YES / 詳細: 11-subunit ctINO80 reconstituted with hexasome
試料支持詳細: 10% Oxygene 90% Argon / グリッドの材料: COPPER / グリッドのサイズ: 200 divisions/in. / グリッドのタイプ: Quantifoil R2/1
急速凍結装置: FEI VITROBOT MARK IV / 凍結剤: ETHANE / 湿度: 100 % / 凍結前の試料温度: 281 K
詳細: wait time of 5s, blot force at 3, and a blot time of 2s with Whatman blotting paper (Cytiva, CAT No. 10311807)

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電子顕微鏡撮影

実験機器
モデル: Titan Krios / 画像提供: FEI Company
顕微鏡モデル: FEI TITAN KRIOS
電子銃電子線源: FIELD EMISSION GUN / 加速電圧: 300 kV / 照射モード: FLOOD BEAM
電子レンズモード: BRIGHT FIELDBright-field microscopy / 最大 デフォーカス（公称値）: 2000 nm / 最小 デフォーカス（公称値）: 800 nm / アライメント法: COMA FREE
試料ホルダ凍結剤: NITROGEN
試料ホルダーモデル: FEI TITAN KRIOS AUTOGRID HOLDER
撮影電子線照射量: 50.36 e/Å2 / フィルム・検出器のモデル: GATAN K3 (6k x 4k) / 実像数: 15384

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解析

EMソフトウェア
ID名称バージョンカテゴリ
2SerialEM画像取得
4CTFFIND4.1.14CTF補正
7ISOLDE1.4モデルフィッティング
8Coot0.9.7モデルフィッティング
10PHENIX1.20.1モデル精密化
11RELION4初期オイラー角割当
14RELION4３次元再構成
CTF補正タイプ: PHASE FLIPPING AND AMPLITUDE CORRECTION
粒子像の選択選択した粒子像数: 2137460
詳細: Particles were initially picked by WARP to generate an initial model, which was subsequently used for the 3D template picking
3次元再構成解像度: 2.7 Å / 解像度の算出法: OTHER / 粒子像の数: 98967 / 対称性のタイプ: POINT
原子モデル構築プロトコル: OTHER
原子モデル構築
IDPDB-ID 3D fitting-IDAccession codeSource nameタイプ
16FML

6fml

16FMLPDBexperimental model
27OHC

7ohc

17OHCPDBexperimental model
38A5Q

8a5q

18A5QPDBexperimental model

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万見について

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お知らせ

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2022年2月9日: EMDBエントリの付随情報ファイルのフォーマットが新しくなりました

EMDBエントリの付随情報ファイルのフォーマットが新しくなりました

  • EMDBのヘッダファイルのバージョン3が、公式のフォーマットとなりました。
  • これまでは公式だったバージョン1.9は、アーカイブから削除されます。

関連情報:EMDBヘッダ

外部リンク:wwPDBはEMDBデータモデルのバージョン3へ移行します

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2020年8月12日: 新型コロナ情報

新型コロナ情報

URL: https://pdbj.org/emnavi/covid19.php

新ページ: EM Navigatorに新型コロナウイルスの特設ページを開設しました。

関連情報:Covid-19情報 / 2020年3月5日: 新型コロナウイルスの構造データ

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2020年3月5日: 新型コロナウイルスの構造データ

新型コロナウイルスの構造データ

関連情報:万見生物種 / 2020年8月12日: 新型コロナ情報

外部リンク:COVID-19特集ページ - PDBj / 今月の分子2020年2月：コロナウイルスプロテーアーゼ

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2019年1月31日: EMDBのIDの桁数の変更

EMDBのIDの桁数の変更

  • EMDBエントリに付与されているアクセスコード(EMDB-ID)は4桁の数字(例、EMD-1234)でしたが、間もなく枯渇します。これまでの4桁のID番号は4桁のまま変更されませんが、4桁の数字を使い切った後に発行されるIDは5桁以上の数字(例、EMD-12345)になります。5桁のIDは2019年の春頃から発行される見通しです。
  • EM Navigator/万見では、接頭語「EMD-」は省略されています。

関連情報:Q: 「EMD」とは何ですか？ / 万見/EM NavigatorにおけるID/アクセスコードの表記

外部リンク:EMDB Accession Codes are Changing Soon! / PDBjへお問い合わせ

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2017年7月12日: PDB大規模アップデート

PDB大規模アップデート

  • 新バージョンのPDBx/mmCIF辞書形式に基づくデータがリリースされました。
  • 今回の更新はバージョン番号が4から5になる大規模なもので、全エントリデータの書き換えが行われる「Remediation」というアップデートに該当します。
  • このバージョンアップで、電子顕微鏡の実験手法に関する多くの項目の書式が改定されました(例：em_softwareなど)。
  • EM NavigatorとYorodumiでも、この改定に基づいた表示内容になります。

外部リンク:wwPDB Remediation / OneDepデータ基準に準拠した、より強化された内容のモデル構造ファイルが、PDBアーカイブで公開されました。

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万見 (Yorodumi)

幾万の構造データを、幾万の視点から

  • 万見(Yorodumi)は、EMDB/PDB/SASBDBなどの構造データを閲覧するためのページです。
  • EM Navigatorの詳細ページの後継、Omokage検索のフロントエンドも兼ねています。

関連情報:EMDB / PDB / SASBDB / 3つのデータバンクの比較 / 万見検索 / 2016年8月31日: 新しいEM Navigatorと万見 / 万見文献 / Jmol/JSmol / 機能・相同性情報 / 新しいEM Navigatorと万見の変更点

他の情報も見る