+Open data
-Basic information
Entry | Database: PDB / ID: 8wtz | ||||||
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Title | potassium outward rectifier channel SKOR | ||||||
Components | Potassium channel SKOR | ||||||
Keywords | METAL TRANSPORT / Potassium transport / Cryo-EM / ion channel / tetramer | ||||||
Function / homology | Function and homology information potassium ion homeostasis / outward rectifier potassium channel activity / monoatomic ion channel complex / potassium ion transport Similarity search - Function | ||||||
Biological species | Arabidopsis thaliana (thale cress) | ||||||
Method | ELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 3.1 Å | ||||||
Authors | Gao, X. / Sun, T. / Lu, Y. / Jia, Y. / Xu, X. / Zhang, Y. / Fu, P. / Yang, G. | ||||||
Funding support | China, 1items
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Citation | Journal: Plant Commun / Year: 2024 Title: Structural changes in the conversion of an Arabidopsis outward-rectifying K channel into an inward-rectifying channel. Authors: Xudong Gao / Xia Xu / Tengfei Sun / Yuhan Lu / Yutian Jia / Jiaqi Zhou / Peng Fu / Yanming Zhang / Guanghui Yang / | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 8wtz.cif.gz | 401.2 KB | Display | PDBx/mmCIF format |
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PDB format | pdb8wtz.ent.gz | 305.6 KB | Display | PDB format |
PDBx/mmJSON format | 8wtz.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/wt/8wtz ftp://data.pdbj.org/pub/pdb/validation_reports/wt/8wtz | HTTPS FTP |
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-Related structure data
Related structure data | 37847MC 8wuiC M: map data used to model this data C: citing same article (ref.) |
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Similar structure data | Similarity search - Function & homologyF&H Search |
-Links
-Assembly
Deposited unit |
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1 |
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-Components
#1: Protein | Mass: 95146.195 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Arabidopsis thaliana (thale cress) / Gene: SKOR / Production host: Spodoptera frugiperda (fall armyworm) / References: UniProt: Q9M8S6 #2: Chemical | ChemComp-PC1 / Has ligand of interest | N | |
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-Experimental details
-Experiment
Experiment | Method: ELECTRON MICROSCOPY |
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EM experiment | Aggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction |
-Sample preparation
Component | Name: SKOR / Type: COMPLEX / Entity ID: #1 / Source: RECOMBINANT |
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Source (natural) | Organism: Arabidopsis thaliana (thale cress) |
Source (recombinant) | Organism: Spodoptera frugiperda (fall armyworm) |
Buffer solution | pH: 7.4 |
Specimen | Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES |
Vitrification | Cryogen name: ETHANE |
-Electron microscopy imaging
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |
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Microscopy | Model: FEI TITAN KRIOS |
Electron gun | Electron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM |
Electron lens | Mode: DIFFRACTION / Nominal defocus max: 1800 nm / Nominal defocus min: 1500 nm |
Image recording | Electron dose: 50 e/Å2 / Film or detector model: GATAN K3 (6k x 4k) |
-Processing
EM software | Name: PHENIX / Version: 1.20.1_4487: / Category: model refinement | ||||||||||||||||||||||||
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CTF correction | Type: PHASE FLIPPING AND AMPLITUDE CORRECTION | ||||||||||||||||||||||||
3D reconstruction | Resolution: 3.1 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 153828 / Symmetry type: POINT | ||||||||||||||||||||||||
Refine LS restraints |
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