+Open data
-Basic information
Entry | Database: EMDB / ID: EMD-37855 | |||||||||
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Title | SKOR D312N L271P double mutation | |||||||||
Map data | ||||||||||
Sample |
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Keywords | Potassium transport / Cryo-EM / ion channel / METAL TRANSPORT | |||||||||
Function / homology | Function and homology information outward rectifier potassium channel activity / potassium ion homeostasis / monoatomic ion channel complex / potassium ion transport Similarity search - Function | |||||||||
Biological species | Arabidopsis thaliana (thale cress) | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 3.4 Å | |||||||||
Authors | Gao X / Sun T / Lu Y / Jia Y / Xu X / Zhang Y / Fu P / Yang G | |||||||||
Funding support | China, 1 items
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Citation | Journal: Plant Commun / Year: 2024 Title: Structural changes in the conversion of an Arabidopsis outward-rectifying K channel into an inward-rectifying channel. Authors: Xudong Gao / Xia Xu / Tengfei Sun / Yuhan Lu / Yutian Jia / Jiaqi Zhou / Peng Fu / Yanming Zhang / Guanghui Yang / | |||||||||
History |
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-Structure visualization
Supplemental images |
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-Downloads & links
-EMDB archive
Map data | emd_37855.map.gz | 78.9 MB | EMDB map data format | |
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Header (meta data) | emd-37855-v30.xml emd-37855.xml | 14.1 KB 14.1 KB | Display Display | EMDB header |
Images | emd_37855.png | 27.9 KB | ||
Filedesc metadata | emd-37855.cif.gz | 5.8 KB | ||
Others | emd_37855_half_map_1.map.gz emd_37855_half_map_2.map.gz | 77.5 MB 77.5 MB | ||
Archive directory | http://ftp.pdbj.org/pub/emdb/structures/EMD-37855 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-37855 | HTTPS FTP |
-Related structure data
Related structure data | 8wuiMC 8wtzC M: atomic model generated by this map C: citing same article (ref.) |
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Similar structure data | Similarity search - Function & homologyF&H Search |
-Links
EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
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Related items in Molecule of the Month |
-Map
File | Download / File: emd_37855.map.gz / Format: CCP4 / Size: 83.7 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||
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Voxel size | X=Y=Z: 1.04 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Half map: #2
File | emd_37855_half_map_1.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Half map: #1
File | emd_37855_half_map_2.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Sample components
-Entire : SKOR double mutation tetramer
Entire | Name: SKOR double mutation tetramer |
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Components |
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-Supramolecule #1: SKOR double mutation tetramer
Supramolecule | Name: SKOR double mutation tetramer / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1 |
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Source (natural) | Organism: Arabidopsis thaliana (thale cress) |
-Macromolecule #1: Potassium channel SKOR
Macromolecule | Name: Potassium channel SKOR / type: protein_or_peptide / ID: 1 / Number of copies: 4 / Enantiomer: LEVO |
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Source (natural) | Organism: Arabidopsis thaliana (thale cress) |
Molecular weight | Theoretical: 95.260367 KDa |
Recombinant expression | Organism: Spodoptera frugiperda (fall armyworm) |
Sequence | String: MDYKDDDDKH MGGSSGGGVS YRSGGESDVE LEDYEVDDFR DGIVESRGNR FNPLTNFLGL DFAGGSGGKF TVINGIRDIS RGSIVHPDN RWYKAWTMFI LIWALYSSFF TPLEFGFFRG LPENLFILDI AGQIAFLVDI VLTFFVAYRD SRTYRMIYKR S SIALRYLK ...String: MDYKDDDDKH MGGSSGGGVS YRSGGESDVE LEDYEVDDFR DGIVESRGNR FNPLTNFLGL DFAGGSGGKF TVINGIRDIS RGSIVHPDN RWYKAWTMFI LIWALYSSFF TPLEFGFFRG LPENLFILDI AGQIAFLVDI VLTFFVAYRD SRTYRMIYKR S SIALRYLK STFIIDLLAC MPWDIIYKAA GEKEEVRYLL LIRLYRVHRV ILFFHKMEKD IRINYLFTRI VKLIFVELYC TH TAACIFY YLATTLPASQ EGYTWIGSLK LGDYSYSKFR EIDPWTRYTT SMYFAVVTMA TVGYGDIHAV NMREMIFAMV YIS FNMILG AYLIGNMTAL IVKGSKTERF RDKMADIMRY MNRNKLGRNI RGQITGHLRL QYESSYTEAA VLQDIPVSIR AKIA QTLYL PYIEKVPLFR GCSSEFINQI VIRLHEEFFL PGEVIMEQGS VVDQLYFVCH GVLEEIGITK DGSEEIVAVL QPDHS FGEI SILCNIPQPY TVRVAELCRI LRLDKQSFMN ILEIFFHDGR RILNNLLEGK ESNVRIKQLE SDITFHISKQ EAELAL KLN SAAFYGDLYQ LKSLIRAGGD PNKTDYDGRS PLHLAASRGY EDITLYLIQE SVDVNIKDKL GSTPLLEAIK NGNDRVA AL LVKEGATLNI ENAGTFLCTV VAKGDSDFLK RLLSNGIDPN SKDYDHRTPL HVAASEGFYV LAIQLVEASA NVLAKDRW G NTPLDEALGC GNKMLIKLLE DAKNSQISSF PSGSKEPKDK VYKKKCTVYF SHPGDSKEKR RRGIVLWVPR SIEELIRTA KEQLNVPEAS CVLSEDEAKI IDVDLISDGQ KLYLAVET UniProtKB: Potassium channel SKOR |
-Macromolecule #2: 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE
Macromolecule | Name: 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE / type: ligand / ID: 2 / Number of copies: 14 / Formula: PC1 |
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Molecular weight | Theoretical: 790.145 Da |
Chemical component information | ChemComp-PC1: |
-Experimental details
-Structure determination
Method | cryo EM |
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Processing | single particle reconstruction |
Aggregation state | particle |
-Sample preparation
Buffer | pH: 7.4 |
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Vitrification | Cryogen name: ETHANE |
-Electron microscopy
Microscope | FEI TITAN KRIOS |
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Electron beam | Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: DIFFRACTION / Nominal defocus max: 1.8 µm / Nominal defocus min: 1.5 µm |
Image recording | Film or detector model: GATAN K3 (6k x 4k) / Average electron dose: 50.0 e/Å2 |
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |
-Image processing
Startup model | Type of model: NONE |
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Initial angle assignment | Type: ANGULAR RECONSTITUTION |
Final angle assignment | Type: ANGULAR RECONSTITUTION |
Final reconstruction | Resolution.type: BY AUTHOR / Resolution: 3.4 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 97040 |