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Open data
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Basic information
Entry | Database: PDB / ID: 7thg | ||||||
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Title | Crystal Structure Of Human NADH-Cytochrome B5 Reductase | ||||||
![]() | NADH-cytochrome b5 reductase 3 soluble form | ||||||
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Function / homology | ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Zheng, A. / Thibodeau, P.H. | ||||||
Funding support | ![]()
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![]() | ![]() Title: Structure Of wild-type Human NADH-Cytochrome B5 Reductase and mutants Authors: Zheng, A. / Thibodeau, P.H. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 80.5 KB | Display | ![]() |
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PDB format | ![]() | 49.3 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 7tnvC ![]() 7tswC ![]() 1umkS S: Starting model for refinement C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Unit cell |
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Components
#1: Protein | Mass: 31151.975 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() ![]() |
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#2: Chemical | ChemComp-FAD / ![]() |
Has ligand of interest | Y |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.4 Å3/Da / Density % sol: 48.71 % |
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Crystal grow![]() | Temperature: 293 K / Method: vapor diffusion, sitting drop / Details: PEG 4K 30%, 0.1M Na/K PO4 pH 7.6 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() |
Detector | Type: RIGAKU SATURN 944 / Detector: CCD / Date: Sep 6, 2017 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Resolution: 2.9→49.05 Å / Num. obs: 6909 / % possible obs: 98.6 % / Redundancy: 5.8 % / Biso Wilson estimate: 67.43 Å2 / Rmerge(I) obs: 0.118 / Rpim(I) all: 0.054 / Rrim(I) all: 0.13 / Net I/σ(I): 11.4 |
Reflection shell | Resolution: 2.9→3 Å / Redundancy: 6.03 % / Rmerge(I) obs: 1.207 / Mean I/σ(I) obs: 1.4 / Num. unique obs: 659 / CC1/2: 0.659 / Rpim(I) all: 0.536 / Rrim(I) all: 1.323 / % possible all: 96.63 |
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Processing
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Refinement | Method to determine structure![]() ![]() Starting model: 1UMK Resolution: 2.9→32.41 Å / SU ML: 0.4535 / Cross valid method: FREE R-VALUE / σ(F): 1.34 / Phase error: 35.524 Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 73.08 Å2 | ||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.9→32.41 Å
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Refine LS restraints |
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LS refinement shell |
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