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- PDB-7l99: Crystal structure of BRDT bromodomain 2 in complex with CDD-1302 -

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Basic information

Entry
Database: PDB / ID: 7l99
TitleCrystal structure of BRDT bromodomain 2 in complex with CDD-1302
ComponentsBromodomain testis-specific protein
KeywordsTRANSCRIPTION / Inhibitor Complex
Function / homology
Function and homology information


sperm DNA condensation / male meiotic nuclear division / male meiosis I / regulation of RNA splicing / histone reader activity / RNA splicing / lysine-acetylated histone binding / mRNA processing / histone binding / transcription coactivator activity ...sperm DNA condensation / male meiotic nuclear division / male meiosis I / regulation of RNA splicing / histone reader activity / RNA splicing / lysine-acetylated histone binding / mRNA processing / histone binding / transcription coactivator activity / chromatin remodeling / positive regulation of gene expression / regulation of DNA-templated transcription / nucleus
Similarity search - Function
Bromodomain protein 4, C-terminal / C-terminal domain of bromodomain protein 4 / NET domain superfamily / NET domain profile. / Brdt, bromodomain, repeat II / Brdt, bromodomain, repeat I / NET domain / Bromodomain extra-terminal - transcription regulation / Bromodomain, conserved site / Bromodomain signature. ...Bromodomain protein 4, C-terminal / C-terminal domain of bromodomain protein 4 / NET domain superfamily / NET domain profile. / Brdt, bromodomain, repeat II / Brdt, bromodomain, repeat I / NET domain / Bromodomain extra-terminal - transcription regulation / Bromodomain, conserved site / Bromodomain signature. / Bromodomain / Bromodomain profile. / bromo domain / Bromodomain / Bromodomain-like superfamily
Similarity search - Domain/homology
Chem-JEF / Chem-XWJ / Bromodomain testis-specific protein
Similarity search - Component
Biological speciesHomo sapiens (human)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.9 Å
AuthorsSharma, R. / Yu, Z. / Ku, A.F. / Anglin, J.L. / Ucisik, M.N. / Faver, J.C. / Sankaran, B. / Kim, C. / Matzuk, M.M.
Funding support United States, 1items
OrganizationGrant numberCountry
National Institutes of Health/Eunice Kennedy Shriver National Institute of Child Health & Human Development (NIH/NICHD)P01HD087157 United States
CitationJournal: Proc.Natl.Acad.Sci.USA / Year: 2021
Title: Discovery and characterization of bromodomain 2-specific inhibitors of BRDT.
Authors: Yu, Z. / Ku, A.F. / Anglin, J.L. / Sharma, R. / Ucisik, M.N. / Faver, J.C. / Li, F. / Nyshadham, P. / Simmons, N. / Sharma, K.L. / Nagarajan, S. / Riehle, K. / Kaur, G. / Sankaran, B. / ...Authors: Yu, Z. / Ku, A.F. / Anglin, J.L. / Sharma, R. / Ucisik, M.N. / Faver, J.C. / Li, F. / Nyshadham, P. / Simmons, N. / Sharma, K.L. / Nagarajan, S. / Riehle, K. / Kaur, G. / Sankaran, B. / Storl-Desmond, M. / Palmer, S.S. / Young, D.W. / Kim, C. / Matzuk, M.M.
History
DepositionJan 3, 2021Deposition site: RCSB / Processing site: RCSB
Revision 1.0Jun 30, 2021Provider: repository / Type: Initial release
Revision 1.1Oct 18, 2023Group: Advisory / Data collection ...Advisory / Data collection / Database references / Refinement description
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_initial_refinement_model / pdbx_unobs_or_zero_occ_atoms
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Bromodomain testis-specific protein
B: Bromodomain testis-specific protein
C: Bromodomain testis-specific protein
D: Bromodomain testis-specific protein
hetero molecules


Theoretical massNumber of molelcules
Total (without water)66,26711
Polymers63,1044
Non-polymers3,1637
Water5,801322
1
A: Bromodomain testis-specific protein
hetero molecules


Theoretical massNumber of molelcules
Total (without water)16,2042
Polymers15,7761
Non-polymers4271
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
2
B: Bromodomain testis-specific protein
hetero molecules


Theoretical massNumber of molelcules
Total (without water)16,2042
Polymers15,7761
Non-polymers4271
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
3
C: Bromodomain testis-specific protein
hetero molecules


Theoretical massNumber of molelcules
Total (without water)16,6313
Polymers15,7761
Non-polymers8552
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
4
D: Bromodomain testis-specific protein
hetero molecules


Theoretical massNumber of molelcules
Total (without water)17,2294
Polymers15,7761
Non-polymers1,4533
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Unit cell
Length a, b, c (Å)47.230, 61.970, 99.190
Angle α, β, γ (deg.)90.000, 97.280, 90.000
Int Tables number4
Space group name H-MP1211
Space group name HallP2yb
Symmetry operation#1: x,y,z
#2: -x,y+1/2,-z
Noncrystallographic symmetry (NCS)NCS domain:
IDEns-IDDetails
d_1ens_1(chain "A" and (resid 267 through 268 or resid 270...
d_2ens_1(chain "B" and (resid 267 through 268 or resid 270...
d_3ens_1(chain "C" and (resid 267 through 268 or resid 270...
d_4ens_1(chain "D" and (resid 267 through 268 or resid 270...

NCS domain segments:
Dom-IDComponent-IDEns-IDBeg label comp-IDEnd label comp-IDLabel asym-IDLabel seq-ID
d_11ens_1SERVALA2 - 3
d_12ens_1ALAARGA5 - 8
d_13ens_1ILELEUA13 - 14
d_14ens_1METALAA17 - 19
d_15ens_1HISASPA22 - 34
d_16ens_1ALATYRA37 - 44
d_17ens_1VALVALA46 - 47
d_18ens_1ASNLYSA49 - 57
d_19ens_1LYSASPA59 - 61
d_110ens_1GLUTYRA64 - 65
d_111ens_1ASPALAA67 - 68
d_112ens_1LYSASNA70 - 81
d_113ens_1TYRSERA83 - 108
d_114ens_1ILEPROA110 - 111
d_115ens_1ALAALAA113
d_116ens_1CDDCDDB
d_21ens_1SERVALC1 - 2
d_22ens_1ALAARGC4 - 7
d_23ens_1ILELEUC12 - 13
d_24ens_1METALAC16 - 18
d_25ens_1HISASPC21 - 33
d_26ens_1ALATYRC36 - 43
d_27ens_1VALVALC45 - 46
d_28ens_1ASNLYSC48 - 56
d_29ens_1LYSASPC58 - 60
d_210ens_1GLUTYRC63 - 64
d_211ens_1ASPALAC66 - 67
d_212ens_1LYSASNC69 - 80
d_213ens_1TYRSERC82 - 107
d_214ens_1ILEALAC109 - 111
d_215ens_1CDDCDDD
d_31ens_1SERVALE2 - 3
d_32ens_1ALAARGE5 - 8
d_33ens_1ILELEUE13 - 14
d_34ens_1METALAE17 - 19
d_35ens_1HISASPE22 - 34
d_36ens_1ALATYRE37 - 44
d_37ens_1VALVALE46 - 47
d_38ens_1ASNLYSE49 - 57
d_39ens_1LYSASPE59 - 61
d_310ens_1GLUTYRE64 - 65
d_311ens_1ASPALAE67 - 68
d_312ens_1LYSASNE70 - 81
d_313ens_1TYRSERE83 - 108
d_314ens_1ILEPROE110 - 111
d_315ens_1ALAALAE113
d_316ens_1CDDCDDF
d_41ens_1SERVALH2 - 3
d_42ens_1ALAARGH5 - 8
d_43ens_1ILELEUH13 - 14
d_44ens_1METALAH17 - 19
d_45ens_1HISASPH22 - 34
d_46ens_1ALATYRH37 - 44
d_47ens_1VALVALH46 - 47
d_48ens_1ASNLYSH49 - 57
d_49ens_1LYSASPH59 - 61
d_410ens_1GLUTYRH64 - 65
d_411ens_1ASPALAH67 - 68
d_412ens_1LYSASNH70 - 81
d_413ens_1TYRSERH83 - 108
d_414ens_1ILEPROH110 - 111
d_415ens_1ALAALAH113
d_416ens_1CDDCDDI

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Components

#1: Protein
Bromodomain testis-specific protein / Cancer/testis antigen 9 / CT9 / RING3-like protein


Mass: 15776.041 Da / Num. of mol.: 4 / Fragment: bromodomain 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: BRDT / Production host: Escherichia coli (E. coli) / References: UniProt: Q58F21
#2: Chemical
ChemComp-XWJ / N-[3-(acetylamino)-4-methylphenyl]-3-(4-amino-2-methylphenyl)-1-methyl-1H-indazole-5-carboxamide


Mass: 427.498 Da / Num. of mol.: 6 / Source method: obtained synthetically / Formula: C25H25N5O2 / Feature type: SUBJECT OF INVESTIGATION
#3: Chemical ChemComp-JEF / O-(O-(2-AMINOPROPYL)-O'-(2-METHOXYETHYL)POLYPROPYLENE GLYCOL 500) / JEFFAMINE


Mass: 597.822 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: C30H63NO10
#4: Water ChemComp-HOH / water / Water


Mass: 18.015 Da / Num. of mol.: 322 / Source method: isolated from a natural source / Formula: H2O
Has ligand of interestY

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.28 Å3/Da / Density % sol: 46.09 %
Crystal growTemperature: 298 K / Method: vapor diffusion, hanging drop / pH: 7
Details: 1.1 M sodium malonate, 0.1 M HEPES pH 7.0, 0.5 % v/v Jeffamine ED-2001

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: ALS / Beamline: 8.2.1 / Wavelength: 1 Å
DetectorType: ADSC QUANTUM 315r / Detector: CCD / Date: Dec 5, 2019
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 1 Å / Relative weight: 1
ReflectionResolution: 1.9→44.55 Å / Num. obs: 44413 / % possible obs: 98.19 % / Redundancy: 2 % / Biso Wilson estimate: 26.41 Å2 / CC1/2: 0.986 / Rmerge(I) obs: 0.087 / Net I/σ(I): 8.24
Reflection shellResolution: 1.9→1.96 Å / Rmerge(I) obs: 1.083 / Num. unique obs: 4301 / CC1/2: 0.2

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Processing

Software
NameVersionClassification
PHENIX1.19_4092refinement
iMOSFLMdata reduction
SCALAdata scaling
PHASERphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 7L9A

7l9a


Resolution: 1.9→44.55 Å / SU ML: 0.211 / Cross valid method: FREE R-VALUE / σ(F): 1.33 / Phase error: 21.8553
Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
RfactorNum. reflection% reflection
Rfree0.2049 2199 4.98 %
Rwork0.1703 41966 -
obs0.172 44165 98.22 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
Displacement parametersBiso mean: 34.5 Å2
Refinement stepCycle: LAST / Resolution: 1.9→44.55 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms3613 0 233 322 4168
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.00574014
X-RAY DIFFRACTIONf_angle_d0.91465477
X-RAY DIFFRACTIONf_chiral_restr0.0482559
X-RAY DIFFRACTIONf_plane_restr0.0074687
X-RAY DIFFRACTIONf_dihedral_angle_d13.61881385
Refine LS restraints NCS
Ens-IDDom-IDAuth asym-IDRefine-IDTypeRms dev position (Å)
ens_1d_2AX-RAY DIFFRACTIONTorsion NCS0.87670369801
ens_1d_3AX-RAY DIFFRACTIONTorsion NCS0.958319408218
ens_1d_4AX-RAY DIFFRACTIONTorsion NCS1.00634858257
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
1.9-1.940.30231310.2782490X-RAY DIFFRACTION94.35
1.94-1.990.31431310.2592526X-RAY DIFFRACTION94.59
1.99-2.040.25711320.2372507X-RAY DIFFRACTION95.48
2.04-2.090.28871140.23282604X-RAY DIFFRACTION96.73
2.09-2.150.29521220.21992603X-RAY DIFFRACTION97.39
2.15-2.220.25811360.20912616X-RAY DIFFRACTION98.46
2.22-2.30.25191670.20532619X-RAY DIFFRACTION99.54
2.3-2.390.22691500.1932609X-RAY DIFFRACTION99.32
2.39-2.50.2131390.1882670X-RAY DIFFRACTION99.57
2.5-2.630.21741470.18472644X-RAY DIFFRACTION99.54
2.63-2.80.23851320.17752671X-RAY DIFFRACTION99.43
2.8-3.020.20681310.17362647X-RAY DIFFRACTION99.18
3.02-3.320.17141320.1652650X-RAY DIFFRACTION99.25
3.32-3.80.18131590.1332639X-RAY DIFFRACTION99.18
3.8-4.790.14661450.12492712X-RAY DIFFRACTION99.93
4.79-44.550.18121310.15072759X-RAY DIFFRACTION99.45
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
14.129466844290.3595983463141.976902967793.812694789553.262772094723.519123924220.2146634749320.606080637438-0.262184739817-0.152089550537-0.397608171690.2202348002760.172542299346-0.04104189328220.1658600773220.370396919053-0.01246200621070.05419314988670.559386188707-0.08043355316240.2911883642223.87434967881-15.56504115758.18180931168
20.9830429909581.154886607970.3265944216173.04670167321-0.7120553821735.4630406162-0.01448599573770.121424201362-0.272448068880.0150443356160.0122267051841-0.1588470091910.3770828622810.09286973839450.02638708401150.1248558487520.01570002602480.03327503495690.244022003678-0.002191884125120.23398282625913.8202150899-7.6606838473926.5730771336
31.987874014950.09369982691030.5647844765817.208789988185.438377125734.24145326607-0.03656690102580.313666057676-0.189287568122-0.111500639844-0.02111780380280.162179391290.2983771485360.03709747156470.1087706709430.1874228374230.005132123455720.005890426465540.264430864658-0.008406446328170.1852074529337.67066513295-16.801826680120.0158678337
44.711481894311.597567074234.840250540632.817800359251.692627099695.44359442135-0.0375959655768-0.06064653565070.147596591294-0.12602765141-0.1465470597520.272234390613-0.00128124963047-0.6416402903610.188972996420.114329302891-0.02409253859350.02543679953270.316632272664-0.02741339708070.204566128180.719452124659-6.6066102259222.3037561688
51.999923055532.000375601784.070614259362.0001982075-4.033459267622.033836162-0.698300024940.0675844480064-1.12510469192-0.392071051943-0.380608561714-0.03284335146840.572163060772-0.03202881561591.375187814121.093508729680.141447519568-0.1906474530070.243333228882-0.008084897235851.05676429935-8.45143129482-23.003105856214.8831127834
67.64457041793-6.367281999065.866660737695.35881332144-5.027878665284.771690954560.0156583964092-0.425816723853-0.5027277315840.394018568028-0.13473685369-0.3103577150510.3716741143590.2148898813270.0100387100110.334283666010.05103297156080.07788213899250.3650715034590.115914872450.358397422176-10.0423185502-18.247889852636.905229859
71.09849267783-0.6176944402190.8734739723723.310901600040.1028267108626.093979496080.01159895977540.0466100666913-0.270009668587-0.1425916883220.09372201618520.1709585441520.319697822527-0.236592898351-0.0901296169240.1059425879470.008133520977210.0227703265340.23223780442-0.004726215019190.209748066584-20.0924897329-6.8247469467720.4699074416
89.883122431736.00967897606-6.912334745294.6356940384-5.013951414325.508568420910.03571723453880.0647555490119-0.6873818023650.761146548886-0.247226213180.867952726460.47314802418-0.3511664141240.06563628728990.453487113579-0.06659872624020.06525929595490.3438970105220.03546526120960.480626644851-19.5918419238-17.839638088529.571816231
93.151782217282.103500233521.37319529554.2215283144-2.37637270234.461043043120.3856987365660.0685813791418-0.810468122247-0.205170855493-0.09040543890290.1311366810011.131572102220.119342360179-0.02665462216920.5856903679440.02669192309090.09144095149340.1628122284680.03544115471961.06547002196-14.686215276-25.400944839829.6752038264
104.78257206694-5.257839117056.611426243745.71524186795-7.347790022239.271040805320.187287356134-0.0356154836651-0.385618521654-0.1131729639460.06028002296050.4434702619740.3791102259690.193275507804-0.2345074365790.2082481907840.0374676487830.02095429371890.236399864594-0.009341338030740.20350996323-10.3755477675-13.522003991720.840301921
114.47876742021-5.52413912519-4.902379266076.823171354336.037483822745.421342117730.2819501053241.685469301310.926900839652-0.601110006018-0.229153038968-0.00826425590336-0.5686626040410.0802776178727-0.2301742113370.238196432578-0.0359055502122-0.03809280594190.3802632253780.1244254212130.289734118203-10.86732301920.87032334151613.71340018
124.80992650436-4.20180104645.988753095714.30216559758-4.634633116738.24510137043-0.0473540144721-0.30962983456-0.146553428830.185171735818-0.0455787996858-0.1906034008570.1527568536340.2080534161810.09486152722460.1622628828050.006510408112110.02252406422460.3368115562220.05133298284650.2685884502-6.18506985957-7.4516432217928.1311532165
133.96816582156-3.77967091869-5.044971830293.669873969394.5074527978.54103283486-0.197600276850.6449670374070.0603938777292-0.4319533285310.199793497106-0.1712656097080.05925843750470.1808114687320.02125049673720.339419581782-0.04261499000630.04169591154370.3826984844810.02303750625560.271356609007-18.25488910353.32756395682-5.81846366418
144.725680734340.8059730380181.514288151873.735938842921.34413935627.70129007932-0.009585316714360.08583821765030.379028783817-0.2459547205710.00718493541620.0267818601023-0.9152965536-0.2675730794660.0492047145670.3064863965590.04289821069120.04708136771180.2010293964510.05864698890720.214598816237-26.147438103616.471704306710.2130761466
155.49353463823-0.167342852706-2.852351221452.812727339550.01346372719395.374877189260.05444679464350.1038215869940.0621878563144-0.23721834261-0.031904440321-0.160548442851-0.147058414430.331327430922-0.04545025569740.169133871505-0.0348396427888-0.02118632626760.1591185889510.02811416718330.123601385342-19.08388054718.642083686126.94400350707
168.95922663793-4.18940405925-4.573276409184.309600866183.864529426553.763131064630.06795589666150.434599809925-0.529877482467-0.296451909763-0.107797439890.04280833558020.248631418941-0.4147688986540.03393944944680.343565810777-0.03606841513840.0006399256818740.340155851913-0.01761603665720.192946144717-27.01881754161.391837088614.65567502984
174.700524382315.1500854552-4.19484977895.57947528012-4.507082389033.806483314990.204748406104-0.927544039177-0.0497338202360.997352793941-0.1546608552190.325023069456-0.06619972903370.2306094097170.04365649370360.505529688610.04319990286710.05192063655480.4126947014770.04193181135930.373822024331-35.891416162-3.0896202962354.3056672649
186.227763291661.21359611168-1.368204820598.78716973011-5.618270208519.470346595040.188603448795-0.603127978921-0.2422355529030.684633106255-0.329316772804-0.5728393680630.1439963681190.9661400308820.09386301113550.2477638245910.0539404264464-0.0543891926280.293227274288-0.03447757718530.178395369977-22.16471467558.3661528391248.4699833033
192.36966789740.793273238239-0.2455647900746.35326310849-3.23941818227.953819044670.0489475383722-0.04520909276840.3092574993330.3695424661060.1681538484040.223876496459-0.948501216819-0.126964067071-0.1958789334250.3121805491690.04448188124890.004810712021640.209467939334-0.02678066290730.252171144724-31.989556716416.747819899836.90263543
205.522796662943.97187040789-4.914977779822.8941068552-3.519823087014.388053307330.455918041247-1.137819605270.59209530781.10304376783-0.319481044590.447840039112-0.403516223082-0.118905118501-0.1650670021870.4238362071170.03949898212940.07330188594410.430660520447-0.07159780296890.291530930523-37.72457616278.7298479803850.8878705648
214.729542941911.57109055136-3.736440967322.57534464928-1.429468289737.998662397640.0947120101283-0.1589778840990.0394817086320.4565460686810.05098862247030.327062563864-0.0250304508491-0.42938034208-0.1419083101010.1897049867620.02407615404390.03536012588670.1936839380540.01025198660420.183850019386-36.702216133.4755300908542.3444941831
222.924569947410.1105114084522.041087526363.500575068732.956002376914.23597822438-0.2756326296090.4904399389220.749668395049-0.05659052761070.140852632888-1.02172234465-0.9604478300951.07279142645-0.04003699655010.25056794527-0.02243671786080.02333810507250.28264632850.05161122324620.226592963051-21.08301882348.9231857751130.702624867
234.432921363553.36301386908-3.921466978588.19410193388-5.709649099368.40639469239-0.221224588424-0.204889213495-0.369227061935-0.02600362910480.0427210640603-0.06823465783110.7971910298660.1923265612380.1982939607350.2677788832980.0243231611772-0.005121038598140.1688212949560.004410722468730.164830558335-26.6017720925-1.8184046473543.5918713212
Refinement TLS group

Refine-ID: X-RAY DIFFRACTION

IDRefine TLS-IDSelection detailsAuth asym-IDLabel asym-IDAuth seq-IDLabel seq-ID
11chain 'A' and (resid 267 through 282 )AA267 - 2822 - 17
22chain 'A' and (resid 283 through 317 )AA283 - 31718 - 52
33chain 'A' and (resid 318 through 350 )AA318 - 35053 - 85
44chain 'A' and (resid 351 through 376 )AA351 - 37686 - 111
55chain 'A' and (resid 377 through 378 )AA377 - 378112 - 113
66chain 'B' and (resid 267 through 282 )BC267 - 2821 - 16
77chain 'B' and (resid 283 through 317 )BC283 - 31717 - 51
88chain 'B' and (resid 318 through 326 )BC318 - 32652 - 60
99chain 'B' and (resid 327 through 332 )BC327 - 33261 - 66
1010chain 'B' and (resid 333 through 350 )BC333 - 35067 - 84
1111chain 'B' and (resid 351 through 355 )BC351 - 35585 - 89
1212chain 'B' and (resid 356 through 376 )BC356 - 37690 - 110
1313chain 'C' and (resid 267 through 283 )CE267 - 2832 - 18
1414chain 'C' and (resid 284 through 317 )CE284 - 31719 - 52
1515chain 'C' and (resid 318 through 355 )CE318 - 35553 - 90
1616chain 'C' and (resid 356 through 377 )CE356 - 37791 - 112
1717chain 'D' and (resid 266 through 282 )DH266 - 2821 - 17
1818chain 'D' and (resid 283 through 294 )DH283 - 29418 - 29
1919chain 'D' and (resid 295 through 317 )DH295 - 31730 - 52
2020chain 'D' and (resid 318 through 326 )DH318 - 32653 - 61
2121chain 'D' and (resid 327 through 350 )DH327 - 35062 - 85
2222chain 'D' and (resid 351 through 355 )DH351 - 35586 - 90
2323chain 'D' and (resid 356 through 377 )DH356 - 37791 - 112

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