+Open data
-Basic information
Entry | Database: PDB / ID: 7ezg | ||||||
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Title | The structure of the human METTL6 enzyme in complex with SAH | ||||||
Components | tRNA N(3)-methylcytidine methyltransferase METTL6 | ||||||
Keywords | Methyltransferase / M3C / tRNA modifications / cocrystal / METTL6 / TOXIN | ||||||
Function / homology | Function and homology information tRNA (cytidine-3-)-methyltransferase activity / tRNA methylation / tRNA modification / Transferases; Transferring one-carbon groups; Methyltransferases / enzyme binding / nucleus / cytoplasm Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.9 Å | ||||||
Authors | Xie, W. / Chen, R. / Zhou, J. / Liu, L. | ||||||
Funding support | China, 1items
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Citation | Journal: Commun Biol / Year: 2021 Title: Crystal structure of human METTL6, the m 3 C methyltransferase. Authors: Chen, R. / Zhou, J. / Liu, L. / Mao, X.L. / Zhou, X. / Xie, W. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 7ezg.cif.gz | 91.2 KB | Display | PDBx/mmCIF format |
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PDB format | pdb7ezg.ent.gz | 54.1 KB | Display | PDB format |
PDBx/mmJSON format | 7ezg.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ez/7ezg ftp://data.pdbj.org/pub/pdb/validation_reports/ez/7ezg | HTTPS FTP |
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-Related structure data
Related structure data | 6c5bS S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 35785.793 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: METTL6 / Production host: Escherichia coli (E. coli) References: UniProt: Q8TCB7, Transferases; Transferring one-carbon groups; Methyltransferases |
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#2: Chemical | ChemComp-SAH / |
#3: Water | ChemComp-HOH / |
Has ligand of interest | Y |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 3.12 Å3/Da / Density % sol: 60.6 % |
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Crystal grow | Temperature: 298 K / Method: vapor diffusion, sitting drop / pH: 9 / Details: PEGRX F7?25% PEG1500?0.1M BIS-Tris pH9.0?0.1M NaCl |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: SYNCHROTRON / Site: SSRF / Beamline: BL19U1 / Wavelength: 0.979 Å |
Detector | Type: DECTRIS PILATUS3 6M / Detector: PIXEL / Date: Jun 28, 2020 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.979 Å / Relative weight: 1 |
Reflection | Resolution: 1.899→50 Å / Num. obs: 35491 / % possible obs: 100 % / Redundancy: 38.5 % / Biso Wilson estimate: 29.02 Å2 / CC1/2: 0.998 / Rmerge(I) obs: 0.19 / Net I/σ(I): 28 |
Reflection shell | Resolution: 1.9→1.97 Å / Rmerge(I) obs: 1.76 / Mean I/σ(I) obs: 2 / Num. unique obs: 3525 / CC1/2: 0.75 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 6C5B Resolution: 1.9→25.3 Å / SU ML: 0.2014 / Cross valid method: FREE R-VALUE / σ(F): 1.36 / Phase error: 20.5623 / Stereochemistry target values: CDL v1.2
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 37.01 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.9→25.3 Å
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Refine LS restraints |
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LS refinement shell |
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