+Open data
-Basic information
Entry | Database: PDB / ID: 1w34 | ||||||
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Title | FERREDOXIN-NADP REDUCTASE (MUTATION: Y 303 S) | ||||||
Components | FERREDOXIN-NADP REDUCTASEFerredoxin—NADP(+) reductase | ||||||
Keywords | OXIDOREDUCTASE / FAD / FLAVOPROTEIN / FNR / MEMBRANE / NADP / NADP REDUCTASE / PHYCOBILISOME / THYLAKOID | ||||||
Function / homology | Function and homology information ferredoxin-NADP+ reductase / ferredoxin-NADP+ reductase activity / phycobilisome / plasma membrane-derived thylakoid membrane / electron transport chain / NADP binding / flavin adenine dinucleotide binding Similarity search - Function | ||||||
Biological species | ANABAENA SP. (bacteria) | ||||||
Method | X-RAY DIFFRACTION / MOLECULAR REPLACEMENT / Resolution: 1.73 Å | ||||||
Authors | Hermoso, J.A. / Perez-Dorado, I. / Medina, M. / Julvez, M.M. / Sanz-Aparicio, J. / Gomez-Moreno, C. | ||||||
Citation | Journal: Biochemistry / Year: 2005 Title: C-Terminal Tyrosine of Ferredoxin-Nadp(+) Reductase in Hydride Transfer Processes with Nad(P)(+)/H. Authors: Tejero, J. / Perez-Dorado, I. / Maya, C. / Julvez, M.M. / Sanz-Aparicio, J. / Gomez-Moreno, C. / Hermoso, J.A. / Medina, M. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1w34.cif.gz | 85.2 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1w34.ent.gz | 62.1 KB | Display | PDB format |
PDBx/mmJSON format | 1w34.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/w3/1w34 ftp://data.pdbj.org/pub/pdb/validation_reports/w3/1w34 | HTTPS FTP |
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-Related structure data
Related structure data | 1w35C 1w87C 2bsaC 1gjrS S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 34096.777 Da / Num. of mol.: 1 / Fragment: RESIDUES 137-440 / Mutation: YES Source method: isolated from a genetically manipulated source Source: (gene. exp.) ANABAENA SP. (bacteria) / Strain: PCC 7119 / Production host: ESCHERICHIA COLI (E. coli) / References: UniProt: P21890, ferredoxin-NADP+ reductase |
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#2: Chemical | ChemComp-FAD / |
#3: Chemical | ChemComp-SO4 / |
#4: Water | ChemComp-HOH / |
Compound details | ENGINEERED |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 3 Å3/Da / Density % sol: 60 % |
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Crystal grow | pH: 5.5 / Details: pH 5.50 |
-Data collection
Diffraction | Mean temperature: 120 K |
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Diffraction source | Source: ROTATING ANODE / Wavelength: 1.54179 |
Detector | Detector: IMAGE PLATE / Date: Jun 14, 2004 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.54179 Å / Relative weight: 1 |
Reflection | Resolution: 1.73→23.01 Å / Num. obs: 42423 / % possible obs: 99.6 % / Observed criterion σ(I): 0 / Redundancy: 4.6 % / Biso Wilson estimate: 21.5 Å2 / Rmerge(I) obs: 0.06 / Net I/σ(I): 6.7 |
Reflection shell | Resolution: 1.73→1.82 Å / Redundancy: 4.3 % / Rmerge(I) obs: 0.25 / Mean I/σ(I) obs: 2.9 / % possible all: 97.6 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: PDB ENTRY 1GJR Resolution: 1.73→23.01 Å / Rfactor Rfree error: 0.004 / Data cutoff high absF: 1493551.04 / Isotropic thermal model: RESTRAINED / Cross valid method: THROUGHOUT / σ(F): 0 / Details: ELECTRON DENSITY WAS NOT PRESENT FOR RESIDUES 0-8.
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Solvent computation | Solvent model: FLAT MODEL / Bsol: 51.2842 Å2 / ksol: 0.397214 e/Å3 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 23.4 Å2
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Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 1.73→23.01 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 1.73→1.84 Å / Rfactor Rfree error: 0.014 / Total num. of bins used: 6
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Xplor file |
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