+Open data
-Basic information
Entry | Database: PDB / ID: 7ekn | ||||||
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Title | Crystal structure of AF10-ipep complex | ||||||
Components |
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Keywords | PROTEIN BINDING / inhibitor | ||||||
Function / homology | nucleoplasm / cytosol / Protein AF-10 Function and homology information | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.14 Å | ||||||
Authors | Chen, S. / Zhou, Z. | ||||||
Funding support | China, 1items
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Citation | Journal: J Leukoc Biol / Year: 2021 Title: Structural characteristics of coiled-coil regions in AF10-DOT1L and AF10-inhibitory peptide complex. Authors: Zhou, Z. / Kang, S. / Huang, Z. / Zhou, Z. / Chen, S. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 7ekn.cif.gz | 100.3 KB | Display | PDBx/mmCIF format |
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PDB format | pdb7ekn.ent.gz | 63.2 KB | Display | PDB format |
PDBx/mmJSON format | 7ekn.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ek/7ekn ftp://data.pdbj.org/pub/pdb/validation_reports/ek/7ekn | HTTPS FTP |
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-Related structure data
Related structure data | 7edpSC S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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2 |
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3 |
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4 |
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Unit cell |
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-Components
#1: Protein/peptide | Mass: 5018.727 Da / Num. of mol.: 4 / Source method: obtained synthetically / Source: (synth.) Homo sapiens (human) #2: Protein/peptide | Mass: 5007.853 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: MLLT10 / Production host: Escherichia coli (E. coli) / References: UniProt: Q5JT35 #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.14 Å3/Da / Density % sol: 42.52 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / Details: PEG4000, Tris8.5, Sodium acetate |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: SYNCHROTRON / Site: SSRF / Beamline: BL18U1 / Wavelength: 0.9788 Å |
Detector | Type: DECTRIS PILATUS3 6M / Detector: PIXEL / Date: Mar 1, 2015 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9788 Å / Relative weight: 1 |
Reflection | Resolution: 2.14→50 Å / Num. obs: 17862 / % possible obs: 97.5 % / Redundancy: 3.8 % / Biso Wilson estimate: 19.38 Å2 / Rmerge(I) obs: 0.129 / Rpim(I) all: 0.129 / Rrim(I) all: 0.182 / Net I/σ(I): 10.2 |
Reflection shell | Resolution: 2.14→2.31 Å / Num. unique obs: 30000 / CC1/2: 0.648 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 7EDP Resolution: 2.14→45.01 Å / SU ML: 0.2943 / Cross valid method: FREE R-VALUE / σ(F): 1.98 / Phase error: 26.5325 Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 23.98 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.14→45.01 Å
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Refine LS restraints |
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LS refinement shell |
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