+Open data
-Basic information
Entry | Database: PDB / ID: 2xus | ||||||
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Title | Crystal Structure of the BRMS1 N-terminal region | ||||||
Components | BREAST CANCER METASTASIS-SUPPRESSOR 1 | ||||||
Keywords | PROTEIN BINDING / BREAST CANCER METASTASIS | ||||||
Function / homology | Function and homology information positive regulation of anoikis / positive regulation of protein deacetylation / negative regulation of stem cell population maintenance / positive regulation of stem cell population maintenance / Sin3-type complex / negative regulation of NF-kappaB transcription factor activity / NF-kappaB binding / negative regulation of cell migration / HDACs deacetylate histones / negative regulation of transforming growth factor beta receptor signaling pathway ...positive regulation of anoikis / positive regulation of protein deacetylation / negative regulation of stem cell population maintenance / positive regulation of stem cell population maintenance / Sin3-type complex / negative regulation of NF-kappaB transcription factor activity / NF-kappaB binding / negative regulation of cell migration / HDACs deacetylate histones / negative regulation of transforming growth factor beta receptor signaling pathway / histone deacetylase binding / regulation of apoptotic process / Potential therapeutics for SARS / negative regulation of DNA-templated transcription / apoptotic process / negative regulation of transcription by RNA polymerase II / nucleoplasm / nucleus / cytoplasm Similarity search - Function | ||||||
Biological species | HOMO SAPIENS (human) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.912 Å | ||||||
Authors | Spinola-Amilibia, M. / Rivera, J. / Ortiz-Lombardia, M. / Romero, A. / Neira, J.L. / Bravo, J. | ||||||
Citation | Journal: J.Mol.Biol. / Year: 2011 Title: The Structure of Brms1 Nuclear Export Signal and Snx6 Interacting Region Reveals a Hexamer Formed by Antiparallel Coiled Coils. Authors: Spinola-Amilibia, M. / Rivera, J. / Ortiz-Lombardia, M. / Romero, A. / Neira, J.L. / Bravo, J. #1: Journal: Acta Crystallogr.,Sect.F / Year: 2008 Title: Crystallization and Preliminary X-Ray Diffraction Analysis of a Breast Cancer Metastasis Suppressor 1 Predicted Coiled-Coil Region. Authors: Spinola-Amilibia, M. / Rivera, J. / Bravo, J. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2xus.cif.gz | 51.6 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2xus.ent.gz | 38.9 KB | Display | PDB format |
PDBx/mmJSON format | 2xus.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/xu/2xus ftp://data.pdbj.org/pub/pdb/validation_reports/xu/2xus | HTTPS FTP |
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-Related structure data
Related structure data | 2ccfS S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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2 |
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Unit cell |
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Components on special symmetry positions |
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Noncrystallographic symmetry (NCS) | NCS oper: (Code: given Matrix: (0.9533, 0.3021, -0.0059), Vector: |
-Components
#1: Protein/peptide | Mass: 5999.769 Da / Num. of mol.: 2 / Fragment: RESIDUES 51-98 Source method: isolated from a genetically manipulated source Source: (gene. exp.) HOMO SAPIENS (human) / Plasmid: PET28-6XHIS-SMT3/BRMS151-98 / Production host: ESCHERICHIA COLI (E. coli) / Strain (production host): BL21(DE3) / Variant (production host): ROSETTA PLYSS / References: UniProt: Q9HCU9 #2: Chemical | #3: Chemical | ChemComp-SO4 / | #4: Water | ChemComp-HOH / | Sequence details | 1 RESIDUE (SER) INSERTED AT THE N-TERMINUS | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 1.95 Å3/Da / Density % sol: 37 % / Description: NONE |
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Crystal grow | pH: 5.25 Details: 0.1 M SODIUM CITRATE PH 5.25, 1.5 M AMMONIUM SULPHATE |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: ESRF / Beamline: ID14-4 / Wavelength: 1.2785 |
Detector | Type: ADSC QUANTUM 315r / Detector: CCD / Date: Nov 20, 2008 / Details: MIRRORS |
Radiation | Monochromator: ESRF MONOCHROMATOR AND TORODIAL FOCUSING MIRROR Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.2785 Å / Relative weight: 1 |
Reflection | Resolution: 1.91→48.7 Å / Num. obs: 7517 / % possible obs: 99.7 % / Observed criterion σ(I): -3 / Redundancy: 40.4 % / Biso Wilson estimate: 33.3 Å2 / Rmerge(I) obs: 0.04 / Net I/σ(I): 79.3 |
Reflection shell | Resolution: 1.91→2.02 Å / Redundancy: 30.9 % / Rmerge(I) obs: 0.28 / Mean I/σ(I) obs: 14.5 / % possible all: 100 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: PDB ENTRY 2CCF Resolution: 1.912→48.701 Å / Cor.coef. Fo:Fc: 0.949 / Cor.coef. Fo:Fc free: 0.909 / SU B: 7.288 / SU ML: 0.112 / Cross valid method: THROUGHOUT / ESU R: 0.176 / ESU R Free: 0.18 / Stereochemistry target values: MAXIMUM LIKELIHOOD Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS. RESIDUES 51-53 AND 95-98 IN CHAIN A AND 91-98 IN CHAIN B ARE DISORDERED, SER INSERTED AT THE N-TERMINUS IS DISORDERED IN BOTH CHAINS.
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: BABINET MODEL PLUS MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 43.555 Å2
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Refinement step | Cycle: LAST / Resolution: 1.912→48.701 Å
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Refine LS restraints |
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