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- PDB-7a0y: Structure of dimeric sodium proton antiporter NhaA K300R variant,... -

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Basic information

Entry
Database: PDB / ID: 7a0y
TitleStructure of dimeric sodium proton antiporter NhaA K300R variant, at pH 8.2, crystallized with chimeric Fab antibodies
Components
  • (chimeric antibody Fab-F6, ...) x 2
  • Na(+)/H(+) antiporter NhaA
KeywordsMEMBRANE PROTEIN / sodium proton antiporter / transporter / chimeric Fab / antibody
Function / homology
Function and homology information


sodium:proton antiporter activity / regulation of pH / plasma membrane
Similarity search - Function
Na+/H+ antiporter NhaA / Na+/H+ antiporter domain superfamily / Na+/H+ antiporter 1
Similarity search - Domain/homology
1,2-Distearoyl-sn-glycerophosphoethanolamine / 1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE / CARDIOLIPIN / PHOSPHATE ION / Na(+)/H(+) antiporter NhaA
Similarity search - Component
Biological speciesSalmonella enterica subsp. enterica serovar Typhimurium str. LT2 (bacteria)
Gallus gallus (chicken)
Homo sapiens (human)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.45 Å
AuthorsFippel, A. / Mir, S.H. / Lentes, C.J. / Wirth, C. / Hunte, C.
Funding support Germany, European Union, 5items
OrganizationGrant numberCountry
German Research Foundation (DFG)BIOSS - EXC-294 Germany
German Research Foundation (DFG)CIBSS - EXC-2189 - Project ID 390939984 Germany
German Research Foundation (DFG)CRC - 1381 - Project ID 403222702 Germany
German Research Foundation (DFG)CRC - 746 Germany
European Communitys Seventh Framework ProgrammeEDICT - HEALTH-F4-2007-201924European Union
CitationJournal: To Be Published
Title: Molecular determinants for substrate uptake in electrogenic sodium/proton antiporters
Authors: Fippel, A. / Mir, S.H. / Wirth, C. / Gross, N.M. / Lentes, C.J. / Bialas, E. / Frank, F. / Ulbrich, M.H. / Andrade, S.L.A. / Kulik, A. / Hunte, C.
History
DepositionAug 11, 2020Deposition site: PDBE / Processing site: PDBE
Revision 1.0Feb 23, 2022Provider: repository / Type: Initial release
Revision 1.1Jan 31, 2024Group: Data collection / Refinement description
Category: chem_comp_atom / chem_comp_bond / pdbx_initial_refinement_model

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Na(+)/H(+) antiporter NhaA
B: Na(+)/H(+) antiporter NhaA
C: chimeric antibody Fab-F6, heavy chain,chimeric antibody Fab-F6, heavy chain
D: chimeric antibody Fab-F6, light chain,chimeric antibody Fab-F6, light chain
E: chimeric antibody Fab-F6, heavy chain,chimeric antibody Fab-F6, heavy chain
F: chimeric antibody Fab-F6, light chain,chimeric antibody Fab-F6, light chain
hetero molecules


Theoretical massNumber of molelcules
Total (without water)190,54019
Polymers185,4926
Non-polymers5,04713
Water2,648147
1
A: Na(+)/H(+) antiporter NhaA
B: Na(+)/H(+) antiporter NhaA
hetero molecules


  • defined by author&software
  • Evidence: gel filtration, assessed by SEC-MALLS (Lentes, C. J. et al. Molecular characterization of the Na+/H+-antiporter NhaA from Salmonella Typhimurium. PloS one 9, e101575, doi:10.1371/journal.pone.0101575 (2014))
  • 93 kDa, 2 polymers
  • Search similar-shape structures of this assembly by Omokage search (details)
Theoretical massNumber of molelcules
Total (without water)93,00011
Polymers88,0952
Non-polymers4,9059
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area7090 Å2
ΔGint-52 kcal/mol
Surface area32940 Å2
MethodPISA
2


  • Idetical with deposited unit
  • defined by author&software
  • Evidence: gel filtration
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area19370 Å2
ΔGint-145 kcal/mol
Surface area65970 Å2
MethodPISA
3
C: chimeric antibody Fab-F6, heavy chain,chimeric antibody Fab-F6, heavy chain
D: chimeric antibody Fab-F6, light chain,chimeric antibody Fab-F6, light chain
hetero molecules


Theoretical massNumber of molelcules
Total (without water)48,8055
Polymers48,6992
Non-polymers1063
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area3830 Å2
ΔGint-54 kcal/mol
Surface area18590 Å2
MethodPISA
4
E: chimeric antibody Fab-F6, heavy chain,chimeric antibody Fab-F6, heavy chain
F: chimeric antibody Fab-F6, light chain,chimeric antibody Fab-F6, light chain
hetero molecules


Theoretical massNumber of molelcules
Total (without water)48,7343
Polymers48,6992
Non-polymers351
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area3980 Å2
ΔGint-35 kcal/mol
Surface area18900 Å2
MethodPISA
Unit cell
Length a, b, c (Å)112.036, 91.681, 138.287
Angle α, β, γ (deg.)90.000, 110.076, 90.000
Int Tables number4
Space group name H-MP1211
Space group name HallP2yb
Symmetry operation#1: x,y,z
#2: -x,y+1/2,-z

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Components

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Protein , 1 types, 2 molecules AB

#1: Protein Na(+)/H(+) antiporter NhaA / Sodium/proton antiporter NhaA


Mass: 44047.324 Da / Num. of mol.: 2 / Mutation: K300R
Source method: isolated from a genetically manipulated source
Details: K300R variant of NhaA and 5 additional residues at the N-terminus (NLGIL)
Source: (gene. exp.) Salmonella enterica subsp. enterica serovar Typhimurium str. LT2 (bacteria)
Gene: nhaA, STM0039 / Production host: Escherichia coli (E. coli) / Variant (production host): NM554 / References: UniProt: Q8ZRZ3

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Antibody , 2 types, 4 molecules CEDF

#2: Antibody chimeric antibody Fab-F6, heavy chain,chimeric antibody Fab-F6, heavy chain


Mass: 26305.029 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Details: chimeric Fab with avian VH and human CH1 regions,chimeric Fab with avian VH and human CH1 regions
Source: (gene. exp.) Gallus gallus (chicken), (gene. exp.) Homo sapiens (human)
Production host: Escherichia coli (E. coli) / Variant (production host): JM83
#3: Antibody chimeric antibody Fab-F6, light chain,chimeric antibody Fab-F6, light chain


Mass: 22393.760 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Details: chimeric Fab with avian VL and human CL regions,chimeric Fab with avian VL and human CL regions
Source: (gene. exp.) Gallus gallus (chicken), (gene. exp.) Homo sapiens (human)
Production host: Escherichia coli (E. coli) / Variant (production host): JM83

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Non-polymers , 7 types, 160 molecules

#4: Chemical ChemComp-CDL / CARDIOLIPIN / DIPHOSPHATIDYL GLYCEROL / BIS-(1,2-DIACYL-SN-GLYCERO-3-PHOSPHO)-1',3'-SN-GLYCEROL / Cardiolipin


Mass: 1464.043 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: C81H156O17P2 / Comment: phospholipid*YM
#5: Chemical ChemComp-3PH / 1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE / PHOSPHATIDIC ACID / Phosphatidic acid


Mass: 704.998 Da / Num. of mol.: 3 / Source method: obtained synthetically / Formula: C39H77O8P
#6: Chemical ChemComp-PO4 / PHOSPHATE ION / Phosphate


Mass: 94.971 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: PO4
#7: Chemical ChemComp-PG4 / TETRAETHYLENE GLYCOL / Polyethylene glycol


Mass: 194.226 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: C8H18O5 / Comment: precipitant*YM
#8: Chemical ChemComp-3PE / 1,2-Distearoyl-sn-glycerophosphoethanolamine / 3-SN-PHOSPHATIDYLETHANOLAMINE / 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOETHANOLAMINE / Phosphatidylethanolamine


Mass: 748.065 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: C41H82NO8P / Comment: phospholipid*YM
#9: Chemical
ChemComp-CL / CHLORIDE ION / Chloride


Mass: 35.453 Da / Num. of mol.: 4 / Source method: obtained synthetically / Formula: Cl
#10: Water ChemComp-HOH / water / Water


Mass: 18.015 Da / Num. of mol.: 147 / Source method: isolated from a natural source / Formula: H2O

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Details

Has ligand of interestN

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 3.9 Å3/Da / Density % sol: 65.8 %
Crystal growTemperature: 292 K / Method: vapor diffusion, sitting drop / pH: 8.2
Details: 100 mM Tris-HCl pH 8.2, 50 mM BaCl2, 50 mM CaCl2, 30 % PEG400, 0.1 mM 2-aminoperimidine

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: ESRF / Beamline: MASSIF-3 / Wavelength: 0.968 Å
DetectorType: DECTRIS PILATUS3 2M / Detector: PIXEL / Date: Oct 30, 2015
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.968 Å / Relative weight: 1
ReflectionResolution: 2.45→24.74 Å / Num. obs: 57756 / % possible obs: 94.5 % / Redundancy: 13.7 % / Biso Wilson estimate: 56.88 Å2 / CC1/2: 0.995 / Rmerge(I) obs: 0.186 / Net I/σ(I): 12.1
Reflection shellResolution: 2.45→2.77 Å / Redundancy: 14.2 % / Rmerge(I) obs: 1.854 / Mean I/σ(I) obs: 1.7 / Num. unique obs: 2890 / CC1/2: 0.496 / % possible all: 70.2

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Processing

Software
NameVersionClassification
MxCuBEdata collection
PHENIX1.13_2998refinement
STARANISOdata scaling
XDSdata reduction
PHASERphasing
Cootmodel building
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 7A0W

7a0w


Resolution: 2.45→24.74 Å / SU ML: 0.2856 / Cross valid method: FREE R-VALUE / σ(F): 1.33 / Phase error: 32.1039 / Stereochemistry target values: GeoStd + Monomer Library
RfactorNum. reflection% reflection
Rfree0.2414 1746 3.02 %
Rwork0.2034 56006 -
obs0.2046 57752 59.44 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
Displacement parametersBiso mean: 73.68 Å2
Refinement stepCycle: LAST / Resolution: 2.45→24.74 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms11897 0 198 147 12242
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.001612422
X-RAY DIFFRACTIONf_angle_d0.434416905
X-RAY DIFFRACTIONf_chiral_restr0.0391974
X-RAY DIFFRACTIONf_plane_restr0.00362098
X-RAY DIFFRACTIONf_dihedral_angle_d8.86227318
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
2.45-2.530.388730.304270X-RAY DIFFRACTION2.83
2.53-2.640.2415270.3093766X-RAY DIFFRACTION8.21
2.64-2.760.3439420.30561526X-RAY DIFFRACTION16.26
2.76-2.90.31931040.29552764X-RAY DIFFRACTION29.6
2.9-3.080.2971710.26825050X-RAY DIFFRACTION53.85
3.08-3.320.282310.25127722X-RAY DIFFRACTION82.15
3.32-3.650.25373070.21499363X-RAY DIFFRACTION99.59
3.65-4.180.24932940.18429435X-RAY DIFFRACTION100
4.18-5.260.20892960.16239447X-RAY DIFFRACTION100
5.26-24.740.22782710.21179663X-RAY DIFFRACTION99.95
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
11.020234586360.1449629409451.06517851151.58238811923-0.6042148342951.65584065898-0.04885919020190.8738178869150.0384438320353-0.2567697501210.102975361797-0.3758434225750.01601800107830.253616241218-0.05211241213460.5742857529690.06497891851350.09377670785190.809357834388-0.118937798090.30017829575113.12874537552.0419229589131.386062569
22.612742464750.0738050151092-1.440201788393.576802071821.129509254185.470223097760.0005302771673321.13382243829-0.153662512132-1.04343503732-0.105065498226-0.6684605091660.3662805488230.7475456695030.164935914130.6538863805190.1284366040450.1788642284381.00445856186-0.1106867467010.60216610161123.7377062534-1.8111773304328.4736314423
32.01337345917-1.06185136362-0.9078576669651.181695879180.4360882029630.413392963957-0.03660817323260.124862507683-0.774853596806-0.2396984745880.192733539478-0.471435120719-0.0994081865583-0.273961629116-0.1150799098760.787182454858-0.03516789724750.1589807674291.02333883422-0.3096617270930.6200085850770.686069596869-15.053665212829.3226787861
40.696277362177-0.03531862334450.1013709618720.520343949217-0.2020583518241.304207591570.05256223468530.868423290216-0.109240418881-0.525450901097-0.111070369115-0.408317962513-0.04762161601820.207088150511-0.008539330327920.6387905242810.07221200798940.2017432809190.938467074602-0.1189000480030.42316522087314.4417232169-1.538281066225.9756017425
50.719683102727-0.594262646576-0.7448509693363.976704048170.3184046916383.09567031282-0.1076531679271.0628535566-0.224787365741-0.889868171174-0.0458490843254-0.723463949497-0.1545375036990.7595400206740.1302045197510.487079755975-0.01665800201290.1646199520211.0528774381-0.08477185738710.45866525642827.60323757781.8360376002630.6983747138
62.667959369741.23126715152-1.856289319674.52176671737-0.975714036011.29670065943-0.1173218165080.688450563248-0.602240177840.311505288322-0.1948333092170.3173719800160.926436690034-0.2811674192450.386267366790.5963038925130.06681229375930.1138361661530.960145608134-0.1846548608240.47537997274-22.9403365921-6.1474454826626.0477522327
71.28898815319-0.916023512468-1.031196592340.7871374984580.5128481304581.04402283389-0.1182044049711.171836953460.0962879201695-0.544622892219-0.194729411230.304923795908-0.26315407821-0.184845199730.2172090483580.9489142982520.0620088427901-0.09749231724011.596912805510.1302938323550.345139720967-26.578874831711.08861824311.5765170465
82.44750610112-0.190397979452-0.6082230573286.035945914822.267660133633.20355733240.2044051359380.8145522059390.792956087287-0.54293563988-0.269156692346-0.0800131492876-0.5286559106410.2206272086940.07193828573120.8159393819210.00465776431032-0.03065316088541.037582867710.1845392289040.326600349818-8.097575117219.214057504620.8134192056
90.966342617975-0.01540723031490.01880804835051.35297104035-0.02231488879221.90680492495-0.0666253855321.26497725990.307809733515-0.872857100093-0.1287604384660.109510363537-0.204479824137-0.4049892553410.1504946947781.029379837950.0570425709177-0.0541941462191.55357401181-0.03389800057370.254488627709-26.85850359418.0426934838210.7826438367
100.03820090092160.0552963229602-0.389795756230.7668349953831.050971408617.126625986730.2160693390431.082117492490.0298571119321-0.6842988908460.1230760243780.167421140421.44078580224-0.550387161619-0.2940951858350.905121538065-0.0425626912911-0.06141189926261.30800146599-0.1556472414240.343628048044-37.1687985776-0.86120054109711.7910180526
113.5021440867-0.2740384728261.77900491763.0158830633-1.210428234013.850219417640.0986418806667-0.271640160564-0.3067928938620.3547399192710.06651104384210.5099010023080.0251986823064-0.124407949673-0.1296801678880.284123970294-0.03819188675450.0711389524910.0671406017562-0.05858164012310.4813385085428.27737634069-7.8569360448169.2202846849
126.69464331332-3.059184042671.473356259818.42097162306-2.149008935661.35296831390.5554096647380.57266384137-0.431576018036-0.3106402668290.412859371471-0.1181359327520.4072207194050.458942216984-0.5699961388830.231634311963-0.0820285753724-0.00230236204631-0.0413433356997-0.04925192593750.41065396009416.2584898626-6.7369445191860.1733242292
131.195064133180.2381523923260.005245198452152.59789788463-0.1631535432683.02470288561-0.001847562501990.0621584433249-0.3453857898470.165541173770.01197156025370.1671041749330.006256821249640.435718236437-0.04694371470850.1900531927880.03350739275690.0007871509943480.0396242110062-0.06544216387620.26834157001813.5775343172-7.4822881213566.0852448548
146.18203386838-0.724497811605-2.698213542821.404053521481.439439950392.26546151599-0.689803147645-1.35746488393-1.070562012741.414836251550.431582056350.8265894016260.254997428883-0.125496602070.2128323695250.8449718989750.1061716827540.1306515015080.4047391293880.08997577677280.55728073042910.33171671-8.9028286436184.6594183015
157.11669875974-2.95962741441-6.55899458557.585107279886.416591132068.18665633595-0.981223378479-2.8028356573-0.3234084416312.139930058150.3159487958671.57850116076-0.271481449232-0.9188686237550.6505158281121.490899069210.3082876359080.3739273351281.67440544748-0.0992236666130.809991155805-3.008262632920.98102457078299.2128918959
167.54042072751-6.53990976395-5.124829865229.195824385234.795135076056.878289129710.309731851515-0.390320951763-0.712644671360.805370081729-0.693685438081.82380838457-0.2795326408990.006170086328640.4557803743311.18050234415-0.005586023715490.3256174058270.6625526654850.09152465536540.844726770617-0.674763151371-4.6252173547989.4713609321
178.12891088731-3.94800766819-4.73390144276.814187943642.040060923727.48188919446-0.35617478995-0.412316120489-0.5225514781331.24595634636-0.1135493938081.83649076415-0.19918005203-0.4931380011030.4878542519121.125457190350.1744203824970.335865295570.8124471284970.01205966046861.0775582427-5.35369479883-0.51163961055993.5834847769
182.93800207142-0.815284757291-0.6207431103074.197124370941.318183947852.883197508260.0223077755658-0.1040140750370.1514937292540.416347589806-0.1113620782950.343939422493-0.402959442709-0.1550566669910.08297075599740.4026302844090.01637647012560.004378896259330.0703658312125-0.04080332696220.4055239477147.6334862013114.10040019365.4548211037
192.210866186940.2578661847470.7413574076623.09383077038-5.107231890999.02964828337-0.183996156571-1.311488080160.1788562111921.066717602740.2580722794150.281549040527-0.3195866993410.0857201076635-0.05758973074131.752610337270.4660621210620.2567713232231.09615308867-0.1385703632510.6283155003951.7454378564211.093714914899.1483156885
204.661996196211.338484344291.148734568771.5691415079-0.5356691592629.18666346124-0.536250951383-1.244766910070.4753022699291.46268144353-0.1575893091960.389547062441-0.9226049105880.01908741223540.6836253387451.571140343390.3743102757040.1589092826990.986290547724-0.1344394987390.7037280811635.2092371930410.998493559297.3452919821
210.298868422506-0.1107924130180.5885875588570.925640481207-2.52556088517.140897903540.147577550475-0.3762905675290.2474966984810.882376352213-0.0859702303790.101785257312-0.2834853444861.4708974895-0.05826069807212.114690032020.1310117296320.2297329828141.42403857912-0.1580126268630.7072288224751.9464150480519.5817029152103.388897583
226.15179772527-2.06615848596-1.639354369933.522723389650.2713286508623.424814176030.05960241364830.4009966382140.296310538161-0.323099278938-0.106903738835-0.217115361797-0.5747358657970.05844177227780.06544907816070.373930299292-0.0562030747539-0.06805421282050.2207833575770.05640274111670.165756512121-44.551421894518.391464389445.3324135069
232.66057003406-0.517557217191-0.3590973381372.571662327280.3010799209043.739161799340.0122145282626-0.440886729503-0.02007039602810.840621464278-0.0922012709025-0.215311422389-0.01153485352470.0346404337780.04904535051960.426019349292-0.0376596776391-0.1468564462620.162051129095-0.01717069589990.258553043742-59.445257979910.55781904376.2787630151
243.44738903081-0.0325923067166-0.9897696785277.08869006079-2.519860403394.44711313721-0.2477644451170.173499558244-0.854676816197-0.0466063897208-0.1841725984810.04882183293180.5877131340920.2061722020530.3716049933930.2775146940580.02292276726060.1347138602880.239182754352-0.02178687410080.469094268085-39.9061721002-2.0405660488447.6552758297
250.008416650880070.0885260239038-0.1196677229790.765600424666-1.015469140611.34640196980.03249628537650.189381950820.323174402975-0.268474722512-0.25201959272-0.5336501854650.06945121689810.3110551336490.03320365385110.5055280507980.1796263927160.1838331355180.2095036076430.3235856734830.591299999094-46.8818145686-10.675469768865.3689066479
266.00886660289-2.59966890724-0.544100073687.37791586171.706751245122.781055082140.02363637307130.0529340788736-0.1079890946070.081347122084-0.1899227280710.4068445510410.0203785046978-0.08306442321820.1461596927260.300458300245-0.0228173443864-0.01044699530530.08658199196360.0356466505050.262223667206-66.5936347458-3.0466798574471.3948647597
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection details
1X-RAY DIFFRACTION1chain 'A' and (resid 12 through 116 )
2X-RAY DIFFRACTION2chain 'A' and (resid 117 through 171 )
3X-RAY DIFFRACTION3chain 'A' and (resid 172 through 200 )
4X-RAY DIFFRACTION4chain 'A' and (resid 201 through 313 )
5X-RAY DIFFRACTION5chain 'A' and (resid 314 through 383 )
6X-RAY DIFFRACTION6chain 'B' and (resid 11 through 52 )
7X-RAY DIFFRACTION7chain 'B' and (resid 53 through 173 )
8X-RAY DIFFRACTION8chain 'B' and (resid 174 through 218 )
9X-RAY DIFFRACTION9chain 'B' and (resid 219 through 354 )
10X-RAY DIFFRACTION10chain 'B' and (resid 355 through 383 )
11X-RAY DIFFRACTION11chain 'C' and (resid 1 through 44 )
12X-RAY DIFFRACTION12chain 'C' and (resid 45 through 60 )
13X-RAY DIFFRACTION13chain 'C' and (resid 61 through 112 )
14X-RAY DIFFRACTION14chain 'C' and (resid 113 through 128 )
15X-RAY DIFFRACTION15chain 'C' and (resid 129 through 152 )
16X-RAY DIFFRACTION16chain 'C' and (resid 153 through 166 )
17X-RAY DIFFRACTION17chain 'C' and (resid 167 through 223 )
18X-RAY DIFFRACTION18chain 'D' and (resid 1 through 109 )
19X-RAY DIFFRACTION19chain 'D' and (resid 110 through 146 )
20X-RAY DIFFRACTION20chain 'D' and (resid 147 through 184 )
21X-RAY DIFFRACTION21chain 'D' and (resid 185 through 207 )
22X-RAY DIFFRACTION22chain 'E' and (resid 1 through 112 )
23X-RAY DIFFRACTION23chain 'E' and (resid 113 through 223 )
24X-RAY DIFFRACTION24chain 'F' and (resid 1 through 98 )
25X-RAY DIFFRACTION25chain 'F' and (resid 99 through 109 )
26X-RAY DIFFRACTION26chain 'F' and (resid 110 through 209 )

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