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Yorodumi- PDB-1e8v: Structure of the multifunctional paramyxovirus hemagglutinin-neur... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1e8v | |||||||||
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Title | Structure of the multifunctional paramyxovirus hemagglutinin-neuraminidase | |||||||||
Components | HEMAGGLUTININ-NEURAMINIDASE | |||||||||
Keywords | HYDROLASE / SIALIDASE / NEURAMINIDASE / HEMAGGLUTININ | |||||||||
Function / homology | Function and homology information exo-alpha-(2->3)-sialidase activity / exo-alpha-(2->6)-sialidase activity / exo-alpha-(2->8)-sialidase activity / exo-alpha-sialidase / host cell surface receptor binding / symbiont entry into host cell / viral envelope / virion attachment to host cell / host cell plasma membrane / virion membrane / membrane Similarity search - Function | |||||||||
Biological species | NEWCASTLE DISEASE VIRUS | |||||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2 Å | |||||||||
Authors | Crennell, S. / Takimoto, T. / Portner, A. / Taylor, G. | |||||||||
Citation | Journal: Nat.Struct.Biol. / Year: 2000 Title: Crystal Structure of the Multifunctional Paramyxovirus Hemagglutinin-Neuraminidase Authors: Crennell, S. / Takimoto, T. / Portner, A. / Taylor, G. #1: Journal: Virology / Year: 2000 Title: Crystallization of Newcastle Disease Virus Hemagglutinin-Neuraminidase Glycoprotein Authors: Takimoto, T. / Taylor, G.L. / Crennell, S.J. / Scroggs, R.A. / Portner, A. | |||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1e8v.cif.gz | 183.1 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1e8v.ent.gz | 149.5 KB | Display | PDB format |
PDBx/mmJSON format | 1e8v.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/e8/1e8v ftp://data.pdbj.org/pub/pdb/validation_reports/e8/1e8v | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS oper: (Code: given Matrix: (-0.1615, 0.9869, 0.009), Vector: |
-Components
-Protein , 1 types, 2 molecules AB
#1: Protein | Mass: 49856.910 Da / Num. of mol.: 2 / Fragment: HEAD DOMAIN, RESIDUES 124-577 / Source method: isolated from a natural source / Source: (natural) NEWCASTLE DISEASE VIRUS / Strain: KANSAS / References: UniProt: Q9Q2W5, exo-alpha-sialidase |
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-Sugars , 3 types, 6 molecules
#2: Polysaccharide | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose Source method: isolated from a genetically manipulated source | ||
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#4: Sugar | #5: Sugar | |
-Non-polymers , 2 types, 241 molecules
#3: Chemical | #6: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION |
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-Sample preparation
Crystal | Density Matthews: 3.19 Å3/Da / Density % sol: 61.43 % | |||||||||||||||||||||||||
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Crystal grow | pH: 6.5 / Details: pH 6.50 | |||||||||||||||||||||||||
Crystal grow | *PLUS pH: 6.2 / Method: vapor diffusion, hanging drop | |||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: EMBL/DESY, HAMBURG / Beamline: X11 / Type: EMBL/DESY, HAMBURG / Wavelength: 0.909 |
Detector | Detector: IMAGE PLATE |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.909 Å / Relative weight: 1 |
Reflection | Resolution: 2.8→30 Å / Num. obs: 38673 / % possible obs: 94 % / Redundancy: 12.9 % / Rmerge(I) obs: 0.052 / Rsym value: 0.052 |
Reflection | *PLUS Num. measured all: 498619 |
Reflection shell | *PLUS % possible obs: 98 % / Rmerge(I) obs: 0.262 |
-Processing
Software | Name: CNS / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT / Resolution: 2→6 Å / Cross valid method: THROUGHOUT / σ(F): 0
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Refinement step | Cycle: LAST / Resolution: 2→6 Å
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Refine LS restraints |
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