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- PDB-7a0x: Structure of dimeric sodium proton antiporter NhaA, at pH 6.0, cr... -

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Basic information

Entry
Database: PDB / ID: 7a0x
TitleStructure of dimeric sodium proton antiporter NhaA, at pH 6.0, crystallized with chimeric Fab antibodies
Components
  • (chimeric antibody Fab-F6, ...) x 2
  • Na(+)/H(+) antiporter NhaA
KeywordsMEMBRANE PROTEIN / sodium proton antiporter / transporter / antibody / chimeric Fab
Function / homology
Function and homology information


sodium:proton antiporter activity / regulation of pH / plasma membrane
Similarity search - Function
Na+/H+ antiporter NhaA / Na+/H+ antiporter domain superfamily / Na+/H+ antiporter 1
Similarity search - Domain/homology
1,2-Distearoyl-sn-glycerophosphoethanolamine / 1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE / CARDIOLIPIN / PHOSPHATE ION / Na(+)/H(+) antiporter NhaA
Similarity search - Component
Biological speciesSalmonella typhimurium (bacteria)
Gallus gallus (chicken)
Homo sapiens (human)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.37 Å
AuthorsFippel, A. / Lentes, C.J. / Mir, S.H. / Wirth, C. / Hunte, C.
Funding support Germany, European Union, 5items
OrganizationGrant numberCountry
German Research Foundation (DFG)BIOSS - EXC-294 Germany
German Research Foundation (DFG)CIBSS - EXC-2189 - Project ID 390939984 Germany
German Research Foundation (DFG)CRC - 1381 - Project ID ID 403222702 Germany
German Research Foundation (DFG)CRC - 746 Germany
European Communitys Seventh Framework ProgrammeEDICT - HEALTH-F4-2007-201924European Union
CitationJournal: To Be Published
Title: Molecular determinants for substrate uptake in electrogenic sodium/proton antiporters
Authors: Fippel, A. / Mir, S.H. / Wirth, C. / Gross, N.M. / Lentes, C.J. / Bialas, E. / Frank, F. / Ulbrich, M.H. / Andrade, S.L.A. / Kulik, A. / Hunte, C.
History
DepositionAug 11, 2020Deposition site: PDBE / Processing site: PDBE
Revision 1.0Feb 23, 2022Provider: repository / Type: Initial release
Revision 1.1Jan 31, 2024Group: Data collection / Refinement description
Category: chem_comp_atom / chem_comp_bond / pdbx_initial_refinement_model

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

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Assembly

Deposited unit
A: Na(+)/H(+) antiporter NhaA
B: Na(+)/H(+) antiporter NhaA
C: chimeric antibody Fab-F6, heavy chain,chimeric antibody Fab-F6, heavy chain,chimeric antibody Fab-F6, heavy chain,chimeric antibody Fab-F6, heavy chain
D: chimeric antibody Fab-F6, light chain,chimeric antibody Fab-F6, light chain,chimeric antibody Fab-F6, light chain,chimeric antibody Fab-F6, light chain
E: chimeric antibody Fab-F6, heavy chain,chimeric antibody Fab-F6, heavy chain,chimeric antibody Fab-F6, heavy chain,chimeric antibody Fab-F6, heavy chain
F: chimeric antibody Fab-F6, light chain,chimeric antibody Fab-F6, light chain,chimeric antibody Fab-F6, light chain,chimeric antibody Fab-F6, light chain
hetero molecules


Theoretical massNumber of molelcules
Total (without water)189,85521
Polymers184,4156
Non-polymers5,44015
Water3,189177
1
A: Na(+)/H(+) antiporter NhaA
B: Na(+)/H(+) antiporter NhaA
hetero molecules


  • defined by author&software
  • Evidence: gel filtration, assessed by SEC-MALLS (Lentes, C. J. et al. Molecular characterization of the Na+/H+-antiporter NhaA from Salmonella Typhimurium. PloS one 9, e101575, doi:10.1371/journal.pone.0101575 (2014))
  • 91.9 kDa, 2 polymers
  • Search similar-shape structures of this assembly by Omokage search (details)
Theoretical massNumber of molelcules
Total (without water)91,92311
Polymers87,0172
Non-polymers4,9059
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area6330 Å2
ΔGint-60 kcal/mol
Surface area32990 Å2
MethodPISA
2


  • Idetical with deposited unit
  • defined by author&software
  • Evidence: gel filtration
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area20390 Å2
ΔGint-134 kcal/mol
Surface area65780 Å2
MethodPISA
3
C: chimeric antibody Fab-F6, heavy chain,chimeric antibody Fab-F6, heavy chain,chimeric antibody Fab-F6, heavy chain,chimeric antibody Fab-F6, heavy chain
D: chimeric antibody Fab-F6, light chain,chimeric antibody Fab-F6, light chain,chimeric antibody Fab-F6, light chain,chimeric antibody Fab-F6, light chain
hetero molecules


Theoretical massNumber of molelcules
Total (without water)48,7704
Polymers48,6992
Non-polymers712
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area3900 Å2
ΔGint-39 kcal/mol
Surface area19060 Å2
MethodPISA
4
E: chimeric antibody Fab-F6, heavy chain,chimeric antibody Fab-F6, heavy chain,chimeric antibody Fab-F6, heavy chain,chimeric antibody Fab-F6, heavy chain
F: chimeric antibody Fab-F6, light chain,chimeric antibody Fab-F6, light chain,chimeric antibody Fab-F6, light chain,chimeric antibody Fab-F6, light chain
hetero molecules


Theoretical massNumber of molelcules
Total (without water)49,1636
Polymers48,6992
Non-polymers4644
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area4910 Å2
ΔGint-38 kcal/mol
Surface area18980 Å2
MethodPISA
Unit cell
Length a, b, c (Å)113.010, 91.713, 139.997
Angle α, β, γ (deg.)90.000, 109.798, 90.000
Int Tables number4
Space group name H-MP1211
Space group name HallP2yb
Symmetry operation#1: x,y,z
#2: -x,y+1/2,-z

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Components

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Protein , 1 types, 2 molecules AB

#1: Protein Na(+)/H(+) antiporter NhaA / Sodium/proton antiporter NhaA


Mass: 43508.680 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (bacteria)
Strain: LT2 / SGSC1412 / ATCC 700720 / Gene: nhaA, STM0039 / Production host: Escherichia coli (E. coli) / Variant (production host): NM554 / References: UniProt: Q8ZRZ3

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Antibody , 2 types, 4 molecules CEDF

#2: Antibody chimeric antibody Fab-F6, heavy chain,chimeric antibody Fab-F6, heavy chain,chimeric antibody Fab-F6, heavy chain,chimeric antibody Fab-F6, heavy chain


Mass: 26305.029 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Details: chimeric Fab with an avian VH and human CH1 regions,chimeric Fab with an avian VH and human CH1 regions,chimeric Fab with an avian VH and human CH1 regions,chimeric Fab with an avian VH and human CH1 regions
Source: (gene. exp.) Gallus gallus (chicken), (gene. exp.) Homo sapiens (human)
Production host: Escherichia coli (E. coli) / Variant (production host): JM83
#3: Antibody chimeric antibody Fab-F6, light chain,chimeric antibody Fab-F6, light chain,chimeric antibody Fab-F6, light chain,chimeric antibody Fab-F6, light chain


Mass: 22393.760 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Details: chimeric Fab with avian VL and human CL regions,chimeric Fab with avian VL and human CL regions,chimeric Fab with avian VL and human CL regions,chimeric Fab with avian VL and human CL regions
Source: (gene. exp.) Gallus gallus (chicken), (gene. exp.) Homo sapiens (human)
Production host: Escherichia coli (E. coli) / Variant (production host): JM83

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Non-polymers , 8 types, 192 molecules

#4: Chemical ChemComp-CDL / CARDIOLIPIN / DIPHOSPHATIDYL GLYCEROL / BIS-(1,2-DIACYL-SN-GLYCERO-3-PHOSPHO)-1',3'-SN-GLYCEROL / Cardiolipin


Mass: 1464.043 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: C81H156O17P2 / Comment: phospholipid*YM
#5: Chemical ChemComp-3PH / 1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE / PHOSPHATIDIC ACID / Phosphatidic acid


Mass: 704.998 Da / Num. of mol.: 3 / Source method: obtained synthetically / Formula: C39H77O8P
#6: Chemical ChemComp-PO4 / PHOSPHATE ION / Phosphate


Mass: 94.971 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: PO4
#7: Chemical
ChemComp-PG4 / TETRAETHYLENE GLYCOL / Polyethylene glycol


Mass: 194.226 Da / Num. of mol.: 4 / Source method: obtained synthetically / Formula: C8H18O5 / Comment: precipitant*YM
#8: Chemical ChemComp-3PE / 1,2-Distearoyl-sn-glycerophosphoethanolamine / 3-SN-PHOSPHATIDYLETHANOLAMINE / 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOETHANOLAMINE / Phosphatidylethanolamine


Mass: 748.065 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: C41H82NO8P / Comment: phospholipid*YM
#9: Chemical ChemComp-CL / CHLORIDE ION / Chloride


Mass: 35.453 Da / Num. of mol.: 3 / Source method: obtained synthetically / Formula: Cl
#10: Chemical ChemComp-CA / CALCIUM ION


Mass: 40.078 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: Ca
#11: Water ChemComp-HOH / water / Water


Mass: 18.015 Da / Num. of mol.: 177 / Source method: isolated from a natural source / Formula: H2O

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Details

Has ligand of interestN

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 4.1 Å3/Da / Density % sol: 66.77 %
Crystal growTemperature: 292 K / Method: vapor diffusion, sitting drop / pH: 8.2
Details: 100 mM Tris-HCl pH 8.2, 50 mM BaCl2, 50 mM CaCl2, 33 % PEG400, soaked at pH 6.0

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: PETRA III, EMBL c/o DESY / Beamline: P13 (MX1) / Wavelength: 0.976 Å
DetectorType: DECTRIS PILATUS 6M-F / Detector: PIXEL / Date: Oct 14, 2017
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.976 Å / Relative weight: 1
ReflectionResolution: 2.37→24.9 Å / Num. obs: 69454 / % possible obs: 94.4 % / Redundancy: 20.4 % / Biso Wilson estimate: 62.04 Å2 / CC1/2: 0.999 / Rmerge(I) obs: 0.16 / Net I/σ(I): 17.8
Reflection shellResolution: 2.37→2.68 Å / Redundancy: 18.6 % / Rmerge(I) obs: 2.75 / Mean I/σ(I) obs: 1.7 / Num. unique obs: 3474 / CC1/2: 0.509 / % possible all: 64.3

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Processing

Software
NameVersionClassification
MxCuBEdata collection
PHENIX1.13_2998refinement
STARANISOdata scaling
XDSdata reduction
PHASERphasing
Cootmodel building
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 7A0W

7a0w


Resolution: 2.37→24.9 Å / SU ML: 0.3039 / Cross valid method: FREE R-VALUE / σ(F): 1.35 / Phase error: 35.0628 / Stereochemistry target values: GeoStd + Monomer Library
RfactorNum. reflection% reflection
Rfree0.2412 2219 3.2 %
Rwork0.2048 67204 -
obs0.206 69423 63.14 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
Displacement parametersBiso mean: 77.87 Å2
Refinement stepCycle: LAST / Resolution: 2.37→24.9 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms11849 0 222 177 12248
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.001812410
X-RAY DIFFRACTIONf_angle_d0.507116884
X-RAY DIFFRACTIONf_chiral_restr0.04021970
X-RAY DIFFRACTIONf_plane_restr0.00362091
X-RAY DIFFRACTIONf_dihedral_angle_d10.32917305
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
2.37-2.450.436260.3168201X-RAY DIFFRACTION1.9
2.45-2.550.3264110.3164768X-RAY DIFFRACTION7.11
2.55-2.660.4054720.32012019X-RAY DIFFRACTION19.18
2.66-2.80.35031430.31524647X-RAY DIFFRACTION43.66
2.8-2.980.3132250.28177308X-RAY DIFFRACTION68.84
2.98-3.210.30592850.26059503X-RAY DIFFRACTION89.31
3.21-3.530.25764100.22410570X-RAY DIFFRACTION99.75
3.53-4.040.24383780.186610614X-RAY DIFFRACTION100
4.04-5.080.20233120.157510750X-RAY DIFFRACTION100
5.08-24.90.22513770.210610824X-RAY DIFFRACTION99.93
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
12.631721586521.401374313351.306643569562.28554800611-0.4126896956681.37499542214-0.1345558501050.7390587480630.154849277995-0.1434070751930.329830913602-0.1101242712770.05252810825240.23788870562-0.06753585581520.5498003482710.03280007601910.0453575361010.6934141963980.003220295644730.2618973480827.418796660086.732615126132.453389174
22.36252847442-0.7442999985660.6838321270312.033103487240.4771743898711.62952623260.1954358960311.43080086906-0.275179878903-0.705239302028-0.202771696976-0.4327439643110.2507836082480.7890833989630.01764341962760.648182390820.1336875580580.1975691422821.07419425091-0.2217069043540.627601712424.4545662353-5.009358273929.2397519085
32.774949249460.55257789787-1.643100159821.63837504784-0.03422751610681.023558264540.2502165986260.583343974715-1.03152176281-0.6292791276650.173959739584-0.5283637246280.4294057441120.178102747914-0.03627901937050.7852930119990.03281855862060.1446108864230.815957628068-0.2915754201420.431418373054-1.25917596801-11.927285789125.2540228623
41.411610050880.7095115331960.3403252975132.87134051490.611948475382.439330413710.167863235151.10053369749-0.164352685678-0.799561198052-0.0716837772105-0.4882628471550.1577676528880.249464401388-0.05105295508170.740749326030.1117251011060.2329982885390.8652629827-0.08792663506050.4195701401210.53100016981.4707605811525.2789108906
51.06100537939-0.08131580182030.4259147250823.091920376840.534418902752.422328892090.08745620918550.84617066282-0.24445011996-0.80052400115-0.149685584582-0.518017010685-0.1051543589141.107370606430.1428986529590.4972050737830.07910724907250.1712576304110.991400160443-0.08044945495680.4760051308428.4809346798-0.39090338885432.1238652456
63.245918824540.716726926602-0.1053863833144.531498735081.268588353530.411741445074-0.02011924573630.707027195386-0.148444038842-0.302243768741-0.0320469404536-0.0239377911703-0.06169637125080.05159196199580.1273040196810.6281299583020.04765443885180.05962360933770.937278519825-0.02657761542930.247925673858-20.25829897630.80072534126323.1389182255
70.832541713194-0.1360967922-0.8442681903960.9037208803070.5898451133824.224215722970.2261513198231.693789289650.239744591059-0.281054182562-0.3575967635020.297410286505-0.710254630779-0.3991483232770.2466134155571.00185690520.114233578413-0.18273253021.804894823750.235003201370.241890481588-31.416833036312.03820239079.09834103889
81.03905754518-0.2323343403711.188196738893.338662671331.200364494125.2310025227-0.01853329283291.165180913550.967845897498-0.53807911443-0.0311506903438-0.520827501489-0.541316113998-0.03087507042320.1084523786760.953502662604-0.03444375990410.04123053293890.943393378090.1893225635980.410336847168-8.7883837155719.285729836721.5282132799
91.840230436170.6008911750240.3904864502591.98713854923-0.07977406385172.0570355381-0.03739728750731.222101243280.225298790614-0.644913742592-0.07202452042770.034290198787-0.186121050676-0.1341349197970.07823219501891.012934348390.0826681780120.001689808938681.458152797390.09903096424080.1166763097-25.07401506157.4675862618212.8130576803
101.27054619333-0.000977849213833-0.6345911835281.810911848790.8626254252010.744124132456-0.06944944746551.520316659620.143625712177-0.986818452285-0.1918953475310.271021443667-0.407010674979-0.05919157318020.1286961734291.180509569420.0365730459904-0.1712049963281.657438707460.0246611519510.220690602173-34.95785016595.240160764619.41055418918
114.24317204712-0.5202032766170.3725385006643.4594829914-1.496113581163.145147351560.0235268035873-0.156077063917-0.4104037368520.2270118546950.1061672607360.368977616056-0.03289118203120.105336793666-0.1219637364290.3036134649860.007986203251440.02157412380130.0890428832277-0.0522148525580.37298467527912.4038422729-7.9938979453367.1644554273
122.77875780092-2.31951912209-4.549114206152.766049523482.807885746218.14336883239-0.39877324391-0.473067125202-0.4055651509471.102895158490.1242489769511.202083318420.0402657176661-0.4547889687210.2750140508471.181008563370.1001530794040.245251796360.657983916854-0.01065650247920.859115711589-1.02228885479-0.020074760446892.8030941976
132.53948298116-0.218913909342-0.4602603072743.469121451050.1031088163362.58133846724-0.0217764545786-0.008272984493250.0729601668630.192488411681-0.1668226636830.356280348557-0.224745803566-0.2017567770060.1653927564540.3650031875070.0366583548878-0.005649936297280.133814045055-0.04427220509480.3767601111457.9688887987312.865827813663.7568712042
143.39807151623-0.411587607239-0.5192661674712.48311431628-0.3625456150673.1177729287-0.117647926465-0.2806305024070.1388148649210.3981641475210.1710277309860.131145338877-0.359928873343-0.1079748226378.02711554518E-60.4182150562750.03318304406530.02546622173660.173160596136-0.04414684469890.3706558397048.3524732417215.060371659869.6525649897
152.44010856316-0.4133839260841.955915350847.25550247049-5.801993720456.731399090680.212992242555-1.64899263713-0.221579569770.9594752286270.5273003305361.022860584010.5550424868660.182237068787-0.4719228523991.726932339360.1847209085850.09697565535641.375789219440.03143885102090.6003615644570.5421727042136.02841953141105.981703578
162.68761454376-1.295621226111.79966017316.45071586619-5.991609098785.71547978266-0.304051296242-0.199101771620.2809316233851.65679118127-0.1790919175370.0531183459328-0.1656839289860.3019036431350.6258329339671.571677811260.3752439232210.06754083962221.02259316452-0.04912032538710.462081510366.361105640616.747192542296.7123602068
174.532783671233.400435283224.432733642782.558984471113.333177260174.347305477780.803246918647-0.147469980263-0.03684703534751.53075239213-0.583736677213-0.5500039351391.135308317321.01749987964-0.1524328900171.505856211340.239276587391-0.1786758028081.294594932850.07759364795860.78783907508612.872288137313.4311256406102.498375251
184.30528664977-2.183994413533.128135651075.41313430539-4.796223822584.66472109889-0.549649578677-0.765548419427-0.2765373431951.471269875180.373933235280.427323447990.2879938494430.231073146270.3323070568571.335250632950.3455534437270.0495496864650.906498222293-0.1427864398930.6053350703214.3694739192711.97178478694.4387050043
191.34374756671-0.3889978075430.2328627089340.355251459948-0.2852602053886.041146307320.538443784794-0.1579379770880.05712132728920.353197741122-0.718083252989-0.0746887337581-0.05148174324420.2696838123860.270055749622.634457189180.231012722280.09641916451851.58114223180.1316927301720.2712031497597.9500948941915.2538847215110.025754963
201.33471249673-0.01157772735651.125942890080.783782716124-1.483161675563.67463901335-0.393602507456-0.5381666680270.5465771532651.426331228340.1202287435470.235611401207-1.54635050510.174842598210.3412231775222.01921298470.0381763231350.1592498776421.50194138788-0.2299192732880.4368025286923.0777037070923.0795991548102.661142123
216.45747721338-1.54461852983-1.472821692993.342706985640.3462112251563.724623260160.1363607363820.2915837833520.507341242674-0.677179120725-0.135987636052-0.199351451005-0.79774289420.187468005426-0.01538318051450.573691356305-0.101153214995-0.09340598418330.2982079184550.06260993988650.232575642682-45.100623642119.584456288746.0498087973
222.02055314518-0.0612078583943-1.527291008341.066163819590.6812304588412.833543973670.0837918736225-0.03658241554880.109835229290.232227163273-0.05682279135780.00742253295946-0.111328850987-0.040202951072-0.01968995130730.360631612369-0.0132106711394-0.1274356446360.1610022254610.0215364472360.255873369237-55.570928059711.136069496565.0214717949
233.7810438281-0.237092559724-0.3474366729274.09445919856-0.2181445218384.197218146140.162825357697-0.688234385680.2904705649091.59373095467-0.1242180920330.00269981815797-0.292854318685-0.04826005752630.03993334306720.731285842992-0.0347664216667-0.1371270157090.317131028552-0.06659480074720.345680455919-60.083352938310.030681927583.1326726798
244.774290810790.899675681587-1.027586296876.04641450756-2.19057475756.07509487308-0.2454953287460.135080590873-0.4110322459510.121710282572-0.0158953798759-0.1034118500390.1450666967140.3197900789290.2774715142270.246056350302-0.007393046154070.001611408362150.208685141024-0.01017129869470.212217448227-40.4616146174-2.4151194412848.145257162
250.1156665220350.229483016692-0.5924204204620.454998320031-1.185230227813.0029848635-0.229146854518-0.2802352345320.00125177489211-0.382043142952-0.216783961879-0.5089949080670.709763641080.6853571700390.2687277955190.6184665367820.06021406946020.09254517849120.2973384446580.1428231816320.441841217927-47.5869634128-11.218407603865.8128918271
268.7103679317-3.89257208142-1.261484649757.376095352221.206792737192.338961232240.153601275190.04060529289230.1160253295020.0278985351448-0.2283482828760.314515213539-0.0293360587668-0.08121344991340.07971229589360.340456340071-0.0243218249053-0.0353954381650.1500526814040.0217217491710.155373337719-67.1447287029-3.5067439080672.1288711466
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection details
1X-RAY DIFFRACTION1chain 'A' and (resid 11 through 85 )
2X-RAY DIFFRACTION2chain 'A' and (resid 86 through 173 )
3X-RAY DIFFRACTION3chain 'A' and (resid 174 through 218 )
4X-RAY DIFFRACTION4chain 'A' and (resid 219 through 286 )
5X-RAY DIFFRACTION5chain 'A' and (resid 287 through 380 )
6X-RAY DIFFRACTION6chain 'B' and (resid 11 through 85 )
7X-RAY DIFFRACTION7chain 'B' and (resid 86 through 173 )
8X-RAY DIFFRACTION8chain 'B' and (resid 174 through 218 )
9X-RAY DIFFRACTION9chain 'B' and (resid 219 through 313 )
10X-RAY DIFFRACTION10chain 'B' and (resid 314 through 383 )
11X-RAY DIFFRACTION11chain 'C' and (resid 1 through 112 )
12X-RAY DIFFRACTION12chain 'C' and (resid 113 through 223 )
13X-RAY DIFFRACTION13chain 'D' and (resid 1 through 64 )
14X-RAY DIFFRACTION14chain 'D' and (resid 65 through 109 )
15X-RAY DIFFRACTION15chain 'D' and (resid 110 through 124 )
16X-RAY DIFFRACTION16chain 'D' and (resid 125 through 146 )
17X-RAY DIFFRACTION17chain 'D' and (resid 147 through 159 )
18X-RAY DIFFRACTION18chain 'D' and (resid 160 through 182 )
19X-RAY DIFFRACTION19chain 'D' and (resid 183 through 193 )
20X-RAY DIFFRACTION20chain 'D' and (resid 194 through 207 )
21X-RAY DIFFRACTION21chain 'E' and (resid 1 through 83 )
22X-RAY DIFFRACTION22chain 'E' and (resid 84 through 184 )
23X-RAY DIFFRACTION23chain 'E' and (resid 185 through 223 )
24X-RAY DIFFRACTION24chain 'F' and (resid 1 through 98 )
25X-RAY DIFFRACTION25chain 'F' and (resid 99 through 109 )
26X-RAY DIFFRACTION26chain 'F' and (resid 110 through 209 )

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