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- PDB-6z3u: Structure of the CAK complex form Chaetomium thermophilum -

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Basic information

Entry
Database: PDB / ID: 6z3u
TitleStructure of the CAK complex form Chaetomium thermophilum
Components
  • CYCLIN domain-containing protein
  • Protein kinase domain-containing protein
  • RING-type domain-containing protein
KeywordsTRANSCRIPTION / CAK complex / Kinase / TFIIK / cell cycle / trnascription / DNA repair
Function / homology
Function and homology information


transcription factor TFIIK complex / cyclin-dependent protein serine/threonine kinase activator activity / transcription factor TFIIH holo complex / [RNA-polymerase]-subunit kinase / cyclin-dependent protein serine/threonine kinase regulator activity / RNA polymerase II CTD heptapeptide repeat kinase activity / nucleotide-excision repair / protein phosphorylation / regulation of transcription by RNA polymerase II / ATP binding ...transcription factor TFIIK complex / cyclin-dependent protein serine/threonine kinase activator activity / transcription factor TFIIH holo complex / [RNA-polymerase]-subunit kinase / cyclin-dependent protein serine/threonine kinase regulator activity / RNA polymerase II CTD heptapeptide repeat kinase activity / nucleotide-excision repair / protein phosphorylation / regulation of transcription by RNA polymerase II / ATP binding / metal ion binding / nucleus
Similarity search - Function
Cyclin-dependent kinase 7 / Cdk-activating kinase assembly factor MAT1/Tfb3 / Cdk-activating kinase assembly factor MAT1, centre / CDK-activating kinase assembly factor MAT1 / Zinc finger, C3HC4 type (RING finger) / Cyclin, C-terminal domain 2 / Cyclin C-terminal domain / Cyclin/Cyclin-like subunit Ssn8 / Cyclin, N-terminal / Cyclin, N-terminal domain ...Cyclin-dependent kinase 7 / Cdk-activating kinase assembly factor MAT1/Tfb3 / Cdk-activating kinase assembly factor MAT1, centre / CDK-activating kinase assembly factor MAT1 / Zinc finger, C3HC4 type (RING finger) / Cyclin, C-terminal domain 2 / Cyclin C-terminal domain / Cyclin/Cyclin-like subunit Ssn8 / Cyclin, N-terminal / Cyclin, N-terminal domain / Cyclin-like / domain present in cyclins, TFIIB and Retinoblastoma / Cyclin-like superfamily / Zinc finger, RING-type, conserved site / Zinc finger RING-type signature. / Zinc finger RING-type profile. / Zinc finger, RING-type / Zinc finger, RING/FYVE/PHD-type / Serine/threonine-protein kinase, active site / Serine/Threonine protein kinases active-site signature. / Protein kinase domain / Serine/Threonine protein kinases, catalytic domain / Protein kinase, ATP binding site / Protein kinases ATP-binding region signature. / Protein kinase domain profile. / Protein kinase domain / Protein kinase-like domain superfamily
Similarity search - Domain/homology
RNA polymerase II transcription factor B subunit 3 / [RNA-polymerase]-subunit kinase / CYCLIN domain-containing protein
Similarity search - Component
Biological speciesChaetomium thermophilum (fungus)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.6 Å
AuthorsPeissert, S. / Kuper, J. / Kisker, C.
CitationJournal: Proc.Natl.Acad.Sci.USA / Year: 2020
Title: Structural basis for CDK7 activation by MAT1 and Cyclin H.
Authors: Peissert, S. / Schlosser, A. / Kendel, R. / Kuper, J. / Kisker, C.
History
DepositionMay 22, 2020Deposition site: PDBE / Processing site: PDBE
Revision 1.0Dec 9, 2020Provider: repository / Type: Initial release
Revision 1.1Jan 24, 2024Group: Data collection / Database references / Refinement description
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_initial_refinement_model
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: CYCLIN domain-containing protein
B: Protein kinase domain-containing protein
C: RING-type domain-containing protein
D: CYCLIN domain-containing protein
E: Protein kinase domain-containing protein
F: RING-type domain-containing protein
hetero molecules


Theoretical massNumber of molelcules
Total (without water)208,0547
Polymers208,0186
Non-polymers351
Water37821
1
A: CYCLIN domain-containing protein
B: Protein kinase domain-containing protein
C: RING-type domain-containing protein


Theoretical massNumber of molelcules
Total (without water)104,0093
Polymers104,0093
Non-polymers00
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area8590 Å2
ΔGint-36 kcal/mol
Surface area30240 Å2
MethodPISA
2
D: CYCLIN domain-containing protein
E: Protein kinase domain-containing protein
F: RING-type domain-containing protein
hetero molecules


Theoretical massNumber of molelcules
Total (without water)104,0444
Polymers104,0093
Non-polymers351
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area8810 Å2
ΔGint-35 kcal/mol
Surface area30500 Å2
MethodPISA
Unit cell
Length a, b, c (Å)81.436, 85.240, 160.401
Angle α, β, γ (deg.)90.000, 96.931, 90.000
Int Tables number4
Space group name H-MP1211
Space group name HallP2yb
Symmetry operation#1: x,y,z
#2: -x,y+1/2,-z
Noncrystallographic symmetry (NCS)NCS domain:
IDEns-IDDetails
d_1ens_1chain "A"
d_2ens_1(chain "D" and (resid 5 through 262 or resid 312 through 390))
d_1ens_2chain "B"
d_2ens_2(chain "E" and resid 78 through 399)
d_1ens_3chain "C"
d_2ens_3(chain "F" and resid 273 through 338)

NCS domain segments:
Dom-IDComponent-IDEns-IDBeg label comp-IDEnd label comp-IDLabel asym-IDLabel seq-ID
d_11ens_1ILEGLYA1 - 312
d_21ens_1ILETYRD2 - 234
d_22ens_1SERGLYD236 - 314
d_11ens_2PROSERB1 - 322
d_21ens_2PROSERE2 - 323
d_11ens_3ASPGLYC1 - 66
d_21ens_3ASPGLYF2 - 67

NCS ensembles :
ID
ens_1
ens_2
ens_3

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Components

#1: Protein CYCLIN domain-containing protein


Mass: 46931.000 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus)
Gene: CTHT_0069020 / Production host: Escherichia coli (E. coli) / References: UniProt: G0SH78
#2: Protein Protein kinase domain-containing protein


Mass: 49133.215 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus)
Gene: CTHT_0061570 / Production host: Spodoptera frugiperda (fall armyworm) / References: UniProt: G0SFC6
#3: Protein RING-type domain-containing protein


Mass: 7944.815 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus)
Gene: CTHT_0060790 / Production host: Escherichia coli (E. coli) / References: UniProt: G0SF48
#4: Chemical ChemComp-CL / CHLORIDE ION / Chloride


Mass: 35.453 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: Cl
#5: Water ChemComp-HOH / water / Water


Mass: 18.015 Da / Num. of mol.: 21 / Source method: isolated from a natural source / Formula: H2O
Has ligand of interestN

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.66 Å3/Da / Density % sol: 53.7 %
Crystal growTemperature: 293.15 K / Method: vapor diffusion, sitting drop
Details: 100 mM HEPES pH 7.0-8.0, 0.2 M sodium formate, 15-22% PEG 3350.
PH range: 7-8

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: ESRF / Beamline: ID29 / Wavelength: 1.0723 Å
DetectorType: DECTRIS PILATUS3 S 6M / Detector: PIXEL / Date: Dec 5, 2018
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 1.0723 Å / Relative weight: 1
ReflectionResolution: 2.6→75.9 Å / Num. obs: 33808 / % possible obs: 83.4 % / Redundancy: 6.8 % / Biso Wilson estimate: 38.25 Å2 / CC1/2: 0.98 / Rmerge(I) obs: 0.314 / Rpim(I) all: 0.198 / Net I/σ(I): 6.6
Reflection shellResolution: 2.604→2.816 Å / Redundancy: 6.2 % / Rmerge(I) obs: 1.776 / Mean I/σ(I) obs: 1.3 / Num. unique obs: 1690 / CC1/2: 0.249 / Rpim(I) all: 1.164 / % possible all: 64.4

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Processing

Software
NameVersionClassification
BUSTERrefinement
PHENIX1.18rc4_3812refinement
XDSdata reduction
STARANISOdata scaling
PHASERphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 1ua2

1ua2


Resolution: 2.6→75.87 Å / SU ML: 0.2901 / Cross valid method: FREE R-VALUE / σ(F): 1.35 / Phase error: 27.8692
Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
RfactorNum. reflection% reflection
Rfree0.249 1653 4.89 %
Rwork0.2027 32121 -
obs0.205 33774 50.37 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
Displacement parametersBiso mean: 45.61 Å2
Refinement stepCycle: LAST / Resolution: 2.6→75.87 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms11376 0 1 21 11398
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.002511651
X-RAY DIFFRACTIONf_angle_d0.507415740
X-RAY DIFFRACTIONf_chiral_restr0.03781667
X-RAY DIFFRACTIONf_plane_restr0.00342042
X-RAY DIFFRACTIONf_dihedral_angle_d29.15224421
Refine LS restraints NCS
Ens-IDDom-IDAuth asym-IDRefine-IDTypeRms dev position (Å)
ens_1d_2AX-RAY DIFFRACTIONTorsion NCS0.572901775633
ens_2d_2BX-RAY DIFFRACTIONTorsion NCS0.751849955081
ens_3d_2CX-RAY DIFFRACTIONTorsion NCS0.495517563851
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
2.6-2.680.4346210.3104526X-RAY DIFFRACTION9.86
2.68-2.770.3344350.3444651X-RAY DIFFRACTION12.44
2.77-2.870.3744470.3108957X-RAY DIFFRACTION18.04
2.87-2.980.3546780.28811292X-RAY DIFFRACTION24.79
2.98-3.120.3681710.28671686X-RAY DIFFRACTION31.45
3.12-3.280.26661080.27662042X-RAY DIFFRACTION38.6
3.28-3.490.31581240.24792515X-RAY DIFFRACTION47.68
3.49-3.760.28531830.21993215X-RAY DIFFRACTION61.12
3.76-4.130.23091990.18654110X-RAY DIFFRACTION76.97
4.13-4.730.21352440.16644602X-RAY DIFFRACTION86.14
4.73-5.960.2312680.19595081X-RAY DIFFRACTION95.36
5.96-75.870.22542750.17975444X-RAY DIFFRACTION99.5
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
14.601178103820.4107484496250.6943312697684.221492255950.9206380435721.95313221098-0.0885921668432-1.10657651498-0.2560397557170.295178752601-0.0856562274664-0.0773283676074-0.2707206564960.3314762549920.1623209245850.7003231272430.09514172948340.2007812705280.716154538775-0.05774636207530.35731402069765.620890202819.362417113722.4327424277
28.294202679936.620932770686.776807310625.285981707565.412164774675.544265772480.599941586810.027096568004-1.31890682154-0.07635766305660.433720471302-1.473911482070.4320250754580.915740825613-0.855024456160.5253803377120.08342859088320.03669072775550.786167733798-0.07619257718830.61549158177967.94123779610.4098332891493.83763461711
32.54191457533-0.246162870718-0.3557236015261.745803866110.3125953107971.158627281130.03903213813050.10327875101-0.133217424035-0.5329415066120.238948215794-0.3784922111920.1581468708970.773498214888-0.1343039340870.57337712897-0.003622158062690.2370202468220.549959487255-0.1106399466790.27525021328460.121925110714.2674327898-0.6654673269
42.93327059592-0.624014292792-0.5677767818223.36206667528-0.3816840766640.2544096362360.19091317140.610121102443-0.102982210148-0.43864812389-0.251994471340.2862017071060.1844773042850.353934691042-0.1455010243070.590504854254-0.03517931962090.2519975305420.414386592368-0.1636935047960.24103221386849.235227918916.83767216712.88932199743
54.33065664771-1.289051074830.6185085350290.449943119679-0.04100279065610.3996367401610.153362771686-0.3659805878690.940253333689-0.594028916835-0.0321320411279-0.46120139189-0.7708238321610.471834248482-0.4718988152720.972492975064-0.4215146192990.3765090041210.778739717635-0.2012889299970.57557933817968.815551298241.844589424611.7538473438
62.139693855950.4513968020080.6363062196962.898841966311.976863433982.428192712170.1707513054630.513307115784-0.211410945873-0.3267724043120.0272837232075-0.795858613853-0.6049216757470.726877956184-0.1696386972030.365429410999-0.2200735370150.04184642736170.818907438629-0.07810255664340.41869059272270.762651087327.17038651299.54623108128
71.00690337558-0.926160370791-0.7275463800651.25759329596-0.2173610786142.51034949324-0.12135109382-0.2311621103210.297824899603-0.1480770280360.180495125574-0.746787953221-0.337510661371.07433611721-0.297620309010.495725172667-0.2793694403030.2279361217621.03662783094-0.2691005618430.73896388302676.07547981328.69776111427.36050432832
81.105045844810.2295310775331.755029284694.972014303771.283061145092.957941737-0.2111820586880.326670874887-0.425818259477-0.2096548932550.0174975863534-0.2291044414380.9792613620750.5303223797730.03347964771150.846449653884-0.01227306122720.2887113394550.593701834219-0.2346311193810.51416771352861.19283167533.335411432-9.44308189387
94.433131503414.482324560943.921970155185.022849072374.065330776653.491221463960.928389019599-0.7557744254450.04184053406731.37870427604-0.6238306160240.595351124580.286653466045-0.466231879296-0.05273144990850.8360334560730.02875604773130.08286400900580.747417035316-0.1447606884180.40264943124441.0624341209-4.8391794123-0.401107779614
107.17536651517-0.440679715029-3.144049655632.327859341810.241772250776.56817939057-0.5766288646630.383690464213-1.25493408536-0.1574780365240.135606956378-0.05150987454181.766294671120.09070679372190.3579196995340.9899569433330.01807161072770.05877885750450.336690036396-0.07026033999810.50851734338546.65618518051.0266934336722.6870639061
111.14049404052-0.443058565839-0.9766712924730.9121799295770.3603186020281.13968049961-0.01875481060530.153045201822-0.337948234551-0.0649610007182-0.0106304098877-0.009229510820730.236961835036-0.04943340405640.05491325378860.442863448588-0.1493792600290.04975417444520.33085613368-0.1013256061310.10823028892641.876117558617.930386030127.7992149341
121.37258411143-0.0849874112677-0.6730242161480.924732200523-0.03810023820072.036914740530.3150698300890.07555166119980.253963808113-0.08276524437890.033697116703-0.0336712215727-0.493629970736-0.0195710073137-0.00274685396510.546134080147-0.1746050565820.2567870649510.247175367821-0.0488643417340.016080373570235.354595073436.335073435230.000086227
137.797122556432.731806143953.209045167743.01152836502-0.5283162127892.650324524910.382088148915-0.40000897025-0.0373725896183-0.1208072518550.4183253184-0.535145526309-0.7007616733041.06902611584-0.6445658314950.725298549523-0.157251213766-0.01558478573960.858685441132-0.3003329794250.48542620483557.769008674229.509065735337.0504723363
142.8501060578-1.577939575291.088632285341.96765777716-0.3469092961292.214497388660.258571128799-0.04326279010270.4938934644960.131018947081-0.0164503481608-0.458241606343-0.54839210290.7276170233560.08284506709330.747704943924-0.3258404729650.2592421008640.495874916965-0.1392200996990.4210530429660.676889401336.823865602820.2780403207
153.8062962503-3.71633953259-1.115652295467.228581196591.200286522751.34919920503-0.0446803373253-0.3013037525540.7679271378990.2661878604080.0130581427355-0.578024295131-0.3896026112810.0946476874281-0.0707771250830.415322962885-0.00774462374858-0.01630523643810.260765220765-0.1170761025640.33354762686212.979659843438.118783047597.5956235799
161.78619869036-0.931229949967-0.8180487411163.910450253520.9541100849342.5471924078-0.190262996442-0.342536049230.1140081481260.1155190550110.05463012779310.2305003766380.0311605939408-0.2027474629560.1156623729150.243133409130.0887236447473-0.002298065727320.2117475308830.001719467439060.129242438186-2.5628716284132.03801588793.2799415558
170.860995654313-0.65796335241-0.2565531847763.8131958-0.7303118945250.925089821729-0.278203482683-0.474620101337-0.06582111154070.6095819709920.288683699161-0.2005692287950.3438164095390.2417092066930.009058468884160.5306201024250.1642597663530.07409596396510.3989551938840.09897817720560.12990401704216.298862989713.692022505103.322456452
180.381060519532-0.8029805760040.5412555155751.85354222027-0.9649282347540.918538948455-0.131876305147-0.3640052989820.03805806772590.4418975297690.2462726386420.407260587297-0.446349099471-0.421224224922-0.06535951618830.5322708924070.1565254158670.1772605561680.48844247876-0.01236653885190.204330767639-8.0839327752334.0336079072106.584709827
198.92483080999-7.430334949970.1664188839538.672141349411.000595429560.5248262647790.3732655257961.305160550831.06442275203-1.51628658504-0.459295529357-1.21342625438-0.30291143706-0.04310505023430.1634973476180.6380792832030.1695104832270.1612233162760.7561789979710.07237221070070.563538389974-9.935846866652.242484282679.9244669515
203.1147652565-0.2151651845-1.322224141962.91139726131-0.2456851608172.409995658030.1417510581610.1502112048250.711484314019-0.3256912668030.1694988084680.230151360588-0.809628365801-0.248062967455-0.1886781099380.4259038911430.06277515539840.03813644370380.1889158350290.01989838697630.34705065776810.563554235240.40693586975.818081159
211.991315080120.52466783742-0.5487990023051.956792966070.03252233429832.51044389438-0.2277170695130.217778355947-0.0695064660455-0.1394734474640.1266022091680.005722775650580.180941353480.03800972914360.05202730196340.09453964311590.01830959239740.02828524927960.05294978913140.03643415270740.06961833490316.946678418515.057766087162.1178980271
228.576687659273.79385788446-1.14893495412.18463950604-1.609268386543.91945926425-0.9058653940490.906275266369-1.18144924609-1.033259370990.341244974518-0.689047202450.2994745695550.9373727168970.5643678776490.5850323437530.08020269872880.08696741625030.719173458607-0.1396147651970.50459664518332.234744913518.371206763477.0568021359
233.488901901575.209332849643.182221622859.316189328034.307243643683.067550784720.404835366294-0.388540241002-0.697831151781-0.800224010210.0925159808567-1.875343459860.9297380037331.03939855951-0.3924315849560.5336244006540.1572596806490.1559818974010.4430879371520.09054761118470.52802421473828.133738720212.096176071191.8686277944
240.9073103120740.5996042481360.9967890666734.77424981338-0.6269899417761.868506361270.272925177749-0.0725480669639-0.580989236041-0.09211003731390.0491939098827-0.03584632511690.4071583496230.1098053501120.3633277931260.6402493208510.3192217988910.1392852193860.5103494833540.2222273943710.4640671220820.2777184491.5169653266289.3269463693
251.167941039560.4488045490670.5766812981693.18365092035-0.2806591184370.937645655397-0.00996018237828-0.0711182380139-0.09417747179840.379194153307-0.0641737199594-0.07485832346330.200212821519-0.005582272152790.1127516246430.449613802185-0.02888241557440.1515504538340.1081251238230.06126923541420.3286659452537.670051654961.0490716413480.7081461753
260.834414399778-0.122357083834-0.2823584222020.2134692524540.4953902398811.141311452630.152619036160.02014920633940.208102316610.04507482174810.195916139853-0.196081047923-0.1016011839680.1981328429840.2243348923010.3073207489320.1743269961450.1578845913960.2044192552910.008543257250190.17785322611517.627323805111.178237229887.5017133828
272.574836210530.3980582680260.6572788012291.29475060229-0.01224906420561.03961241618-0.0628800421549-0.4811250160170.4250010089590.07815175248180.07908495346960.0167384825443-0.119819960539-0.1970316080840.2580921682450.23053123420.115220650903-0.1512153648320.627304269568-0.03593262120630.58909843533824.115561065429.4811313368100.652401124
Refinement TLS group

Refine-ID: X-RAY DIFFRACTION

IDRefine TLS-IDSelection detailsAuth asym-IDLabel asym-IDAuth seq-IDLabel seq-ID
11chain 'A' and (resid 5 through 24 )AA5 - 241 - 20
22chain 'A' and (resid 25 through 47 )AA25 - 4721 - 43
33chain 'A' and (resid 48 through 147 )AA48 - 14744 - 118
44chain 'A' and (resid 148 through 188 )AA148 - 188119 - 159
55chain 'A' and (resid 189 through 221 )AA189 - 221160 - 192
66chain 'A' and (resid 222 through 248 )AA222 - 248193 - 219
77chain 'A' and (resid 249 through 350 )AA249 - 350220 - 272
88chain 'A' and (resid 351 through 367 )AA351 - 367273 - 289
99chain 'A' and (resid 368 through 390 )AA368 - 390290 - 312
1010chain 'B' and (resid 78 through 124 )BB78 - 1241 - 47
1111chain 'B' and (resid 125 through 233 )BB125 - 23348 - 156
1212chain 'B' and (resid 234 through 399 )BB234 - 399157 - 322
1313chain 'C' and (resid 273 through 282 )CC273 - 2821 - 10
1414chain 'C' and (resid 283 through 338 )CC283 - 33811 - 66
1515chain 'D' and (resid 4 through 48 )DD4 - 481 - 45
1616chain 'D' and (resid 49 through 188 )DD49 - 18846 - 160
1717chain 'D' and (resid 189 through 332 )DD189 - 332161 - 256
1818chain 'D' and (resid 333 through 367 )DD333 - 367257 - 291
1919chain 'D' and (resid 368 through 390 )DD368 - 390292 - 314
2020chain 'E' and (resid 77 through 172 )EE77 - 1721 - 96
2121chain 'E' and (resid 173 through 399 )EE173 - 39997 - 323
2222chain 'F' and (resid 272 through 281 )FF272 - 2811 - 10
2323chain 'F' and (resid 282 through 286 )FF282 - 28611 - 15
2424chain 'F' and (resid 287 through 293 )FF287 - 29316 - 22
2525chain 'F' and (resid 294 through 311 )FF294 - 31123 - 40
2626chain 'F' and (resid 312 through 324 )FF312 - 32441 - 53
2727chain 'F' and (resid 325 through 338 )FF325 - 33854 - 67

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