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- PDB-6z4x: Structure of the CAK complex form Chaetomium thermophilum bound t... -

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Basic information

Entry
Database: PDB / ID: 6z4x
TitleStructure of the CAK complex form Chaetomium thermophilum bound to ATP-gamma-S
Components
  • CYCLIN domain-containing protein
  • Protein kinase domain-containing protein
  • RING-type domain-containing protein
KeywordsTRANSCRIPTION / CAK complex / Kinase / TFIIK / cell cycle / DNA repair
Function / homology
Function and homology information


transcription factor TFIIK complex / cyclin-dependent protein serine/threonine kinase activator activity / transcription factor TFIIH holo complex / [RNA-polymerase]-subunit kinase / cyclin-dependent protein serine/threonine kinase regulator activity / RNA polymerase II CTD heptapeptide repeat kinase activity / nucleotide-excision repair / protein phosphorylation / regulation of transcription by RNA polymerase II / ATP binding ...transcription factor TFIIK complex / cyclin-dependent protein serine/threonine kinase activator activity / transcription factor TFIIH holo complex / [RNA-polymerase]-subunit kinase / cyclin-dependent protein serine/threonine kinase regulator activity / RNA polymerase II CTD heptapeptide repeat kinase activity / nucleotide-excision repair / protein phosphorylation / regulation of transcription by RNA polymerase II / ATP binding / metal ion binding / nucleus
Similarity search - Function
Cyclin-dependent kinase 7 / Cdk-activating kinase assembly factor MAT1/Tfb3 / Cdk-activating kinase assembly factor MAT1, centre / CDK-activating kinase assembly factor MAT1 / Zinc finger, C3HC4 type (RING finger) / Cyclin, C-terminal domain 2 / Cyclin C-terminal domain / Cyclin/Cyclin-like subunit Ssn8 / Cyclin, N-terminal / Cyclin, N-terminal domain ...Cyclin-dependent kinase 7 / Cdk-activating kinase assembly factor MAT1/Tfb3 / Cdk-activating kinase assembly factor MAT1, centre / CDK-activating kinase assembly factor MAT1 / Zinc finger, C3HC4 type (RING finger) / Cyclin, C-terminal domain 2 / Cyclin C-terminal domain / Cyclin/Cyclin-like subunit Ssn8 / Cyclin, N-terminal / Cyclin, N-terminal domain / Cyclin-like / domain present in cyclins, TFIIB and Retinoblastoma / Cyclin-like superfamily / Zinc finger, RING-type, conserved site / Zinc finger RING-type signature. / Zinc finger RING-type profile. / Zinc finger, RING-type / Zinc finger, RING/FYVE/PHD-type / Serine/threonine-protein kinase, active site / Serine/Threonine protein kinases active-site signature. / Protein kinase domain / Serine/Threonine protein kinases, catalytic domain / Protein kinase, ATP binding site / Protein kinases ATP-binding region signature. / Protein kinase domain profile. / Protein kinase domain / Protein kinase-like domain superfamily
Similarity search - Domain/homology
PHOSPHOTHIOPHOSPHORIC ACID-ADENYLATE ESTER / RNA polymerase II transcription factor B subunit 3 / [RNA-polymerase]-subunit kinase / CYCLIN domain-containing protein
Similarity search - Component
Biological speciesChaetomium thermophilum (fungus)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.98 Å
AuthorsPeissert, S. / Kuper, J. / Kisker, C.
CitationJournal: Proc.Natl.Acad.Sci.USA / Year: 2020
Title: Structural basis for CDK7 activation by MAT1 and Cyclin H.
Authors: Peissert, S. / Schlosser, A. / Kendel, R. / Kuper, J. / Kisker, C.
History
DepositionMay 26, 2020Deposition site: PDBE / Processing site: PDBE
Revision 1.0Dec 9, 2020Provider: repository / Type: Initial release
Revision 1.1Jan 24, 2024Group: Data collection / Database references / Refinement description
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_initial_refinement_model
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: CYCLIN domain-containing protein
B: Protein kinase domain-containing protein
C: RING-type domain-containing protein
D: CYCLIN domain-containing protein
E: Protein kinase domain-containing protein
F: RING-type domain-containing protein
hetero molecules


Theoretical massNumber of molelcules
Total (without water)209,14911
Polymers208,0186
Non-polymers1,1315
Water181
1
A: CYCLIN domain-containing protein
B: Protein kinase domain-containing protein
C: RING-type domain-containing protein
hetero molecules


Theoretical massNumber of molelcules
Total (without water)104,5926
Polymers104,0093
Non-polymers5833
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
2
D: CYCLIN domain-containing protein
E: Protein kinase domain-containing protein
F: RING-type domain-containing protein
hetero molecules


Theoretical massNumber of molelcules
Total (without water)104,5575
Polymers104,0093
Non-polymers5482
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Unit cell
Length a, b, c (Å)89.970, 84.903, 154.477
Angle α, β, γ (deg.)90.000, 96.197, 90.000
Int Tables number4
Space group name H-MP1211
Space group name HallP2yb
Symmetry operation#1: x,y,z
#2: -x,y+1/2,-z
Noncrystallographic symmetry (NCS)NCS domain:
IDEns-IDDetails
d_1ens_1chain "A"
d_2ens_1(chain "D" and (resid 5 through 262 or resid 312 through 390))
d_1ens_2chain "B"
d_2ens_2(chain "E" and (resid 78 through 399 or resid 401))
d_1ens_3chain "C"
d_2ens_3(chain "F" and resid 273 through 338)

NCS domain segments:
Dom-IDComponent-IDEns-IDBeg label comp-IDEnd label comp-IDLabel asym-IDLabel seq-ID
d_11ens_1ILEGLYA1 - 312
d_21ens_1ILETYRE2 - 234
d_22ens_1SERGLYE236 - 314
d_11ens_2PROATGB - C1
d_21ens_2PROATGF - G2
d_11ens_3ASPGLYD1 - 66
d_21ens_3ASPGLYH2 - 67

NCS ensembles :
ID
ens_1
ens_2
ens_3

NCS oper:
IDCodeMatrixVector
1given(0.344325020979, -0.00128179606267, 0.938849634887), (0.00216131308473, -0.999995335992, -0.00215794327688), (0.938848022128, 0.00277218186456, -0.344320644682)23.8424545036, -37.2871403617, -34.3964716033
2given(0.348177581782, -0.004100597676, 0.937419626765), (-0.00193781459456, -0.999991444486, -0.00365456290437), (0.93742659255, -0.00054410855946, -0.348182549141)23.9334040062, -37.6037803031, -34.6027180071
3given(0.342723324991, -0.00180448507186, 0.939434652512), (0.000577966524781, -0.999997560969, -0.0021316685247), (0.939436207766, 0.00127353430594, -0.342721446146)23.7135314248, -37.3829602162, -34.3915900966

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Components

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Protein , 3 types, 6 molecules ADBECF

#1: Protein CYCLIN domain-containing protein


Mass: 46931.000 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Chaetomium thermophilum (fungus) / Production host: Escherichia coli (E. coli) / References: UniProt: G0SH78
#2: Protein Protein kinase domain-containing protein


Mass: 49133.215 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Chaetomium thermophilum (fungus) / Production host: Spodoptera frugiperda (fall armyworm) / References: UniProt: G0SFC6
#3: Protein RING-type domain-containing protein


Mass: 7944.815 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Chaetomium thermophilum (fungus) / Production host: Escherichia coli (E. coli) / References: UniProt: G0SF48

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Non-polymers , 4 types, 6 molecules

#4: Chemical ChemComp-AGS / PHOSPHOTHIOPHOSPHORIC ACID-ADENYLATE ESTER / ATP-GAMMA-S / ADENOSINE 5'-(3-THIOTRIPHOSPHATE) / ADENOSINE 5'-(GAMMA-THIOTRIPHOSPHATE) / ADENOSINE-5'-DIPHOSPHATE MONOTHIOPHOSPHATE


Mass: 523.247 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: C10H16N5O12P3S / Feature type: SUBJECT OF INVESTIGATION / Comment: ATP-gamma-S, energy-carrying molecule analogue*YM
#5: Chemical ChemComp-MG / MAGNESIUM ION


Mass: 24.305 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: Mg
#6: Chemical ChemComp-CL / CHLORIDE ION / Chloride


Mass: 35.453 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: Cl
#7: Water ChemComp-HOH / water / Water


Mass: 18.015 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Formula: H2O

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Details

Has ligand of interestY

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 3.63 Å3/Da / Density % sol: 66.11 %
Crystal growTemperature: 293.15 K / Method: vapor diffusion, sitting drop
Details: 100 mM HEPES pH 7.0-8.0, 0.2 M sodium formate, 15-22% PEG 3350

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: BESSY / Beamline: 14.1 / Wavelength: 0.9184 Å
DetectorType: DECTRIS PILATUS3 S 6M / Detector: PIXEL / Date: Apr 18, 2019
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.9184 Å / Relative weight: 1
ReflectionResolution: 2.98→46.65 Å / Num. obs: 29053 / % possible obs: 89.3 % / Redundancy: 3.3 % / Biso Wilson estimate: 44.66 Å2 / CC1/2: 0.952 / Rmerge(I) obs: 0.277 / Rpim(I) all: 0.179 / Net I/σ(I): 5.2
Reflection shellResolution: 2.98→3.23 Å / Redundancy: 3.3 % / Rmerge(I) obs: 0.983 / Num. unique obs: 1703 / CC1/2: 0.409 / Rpim(I) all: 0.634 / % possible all: 67.1

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Processing

Software
NameVersionClassification
BUSTERrefinement
PHENIX1.18rc4_3812refinement
XDSdata reduction
STARANISOdata scaling
PHASERphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 6Z3U

6z3u


Resolution: 2.98→46.65 Å / SU ML: 0.3382 / Cross valid method: FREE R-VALUE / σ(F): 1.34 / Phase error: 25.8318
Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
RfactorNum. reflection% reflection
Rfree0.2464 1468 5.05 %
Rwork0.2028 27581 -
obs0.2051 29049 61.09 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
Displacement parametersBiso mean: 48.72 Å2
Refinement stepCycle: LAST / Resolution: 2.98→46.65 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms11376 0 65 1 11442
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.002311717
X-RAY DIFFRACTIONf_angle_d0.51315844
X-RAY DIFFRACTIONf_chiral_restr0.03981675
X-RAY DIFFRACTIONf_plane_restr0.00352044
X-RAY DIFFRACTIONf_dihedral_angle_d29.22954447
Refine LS restraints NCS
Ens-IDDom-IDAuth asym-IDRefine-IDTypeRms dev position (Å)
ens_1d_2AX-RAY DIFFRACTIONTorsion NCS0.463092590069
ens_2d_2BX-RAY DIFFRACTIONTorsion NCS0.377194529182
ens_3d_2DX-RAY DIFFRACTIONTorsion NCS0.452776748752
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
2.98-3.090.3733330.331495X-RAY DIFFRACTION11.19
3.09-3.210.3352470.27211007X-RAY DIFFRACTION22.31
3.21-3.360.3156580.27651323X-RAY DIFFRACTION29.27
3.36-3.540.25870.25441752X-RAY DIFFRACTION38.85
3.54-3.760.28241320.24222513X-RAY DIFFRACTION55.81
3.76-4.050.26591750.22663136X-RAY DIFFRACTION70.09
4.05-4.450.25922020.18723937X-RAY DIFFRACTION87.12
4.45-5.10.2332350.17634367X-RAY DIFFRACTION96.86
5.1-6.420.23232560.20124491X-RAY DIFFRACTION99.04
6.42-46.650.21392430.1774560X-RAY DIFFRACTION98.28
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
10.2012659556750.606085221018-0.779011638194.04499597363-2.169503646263.03893126709-0.1137268831730.389731251270.337185318784-0.619518257760.245533132206-0.03226364507510.0922758900544-0.976011692266-0.06903933948040.338574063850.1134628266210.06607551862010.9618352703410.2277182246040.493778839227-69.1522705925-22.54412386-23.1744275591
26.4934547021-5.248603147235.247675519824.43608233035-4.852005429727.035534548840.5849543645080.309995512945-0.853241204546-0.178227925144-0.1582857843840.3227076327770.108046923728-0.397432983698-0.233674192740.460146508647-0.1809437220450.01179005620630.8634688012520.2263475381990.582174429203-72.1922837431-41.5819875049-4.64723197278
30.9880818785230.286150708889-0.6468885970751.92648331668-0.2147789830091.71827294667-0.0434179695505-0.329721092348-0.376190768770.5605744747930.1694375910680.0677399890633-0.0845836108149-0.72292707391-0.1095478442890.4238200050760.009061003696430.1569288411310.5891278515220.1602979933210.330261768718-64.4620768675-27.72782770290.0401672799175
45.205321348270.808021683870.8125083462823.757550349891.881496328961.73621649310.366125337813-0.418377270864-0.1380294841870.777147815167-0.00574436730203-0.2151890608210.1149336298590.0174852377367-0.3177094067950.4819500700060.02622385285130.1729956739630.33325567950.1474319794070.312949154231-53.4978348337-25.2772064188-3.12962974105
51.7205718449-0.4578427736950.5274441033970.6422614582530.3408141627020.611413326985-0.07088119586680.05021905221910.8676382442070.00941075785985-0.1625911936590.301846055852-0.358100192212-0.336942947014-0.09881564004550.6671450933290.5258449104170.1883230377410.7785025613140.1358243797060.77819470468-72.5213673872-0.120008228634-12.6250016195
63.60916923533-1.30981838558-1.151158935411.828306029680.2109466264575.31459288135-0.148478785780.0300543794733-0.5180668034230.3168116042280.3768342728530.831445338629-0.534474674177-1.41349828616-0.1618100762130.3467373961770.09934761722930.1398998456060.8005569269060.1379396172380.489899770905-74.6401415671-14.7735181322-10.4830026298
71.06758955494-1.098381575191.602235367523.02227376989-0.5121125350933.106599562820.1196810421660.1729965927270.517371571182-0.03271138309960.0415055072090.8045279755570.0987693729592-0.878948077481-0.09507963968890.3371611805110.1420397880160.04453132767691.22784213190.2779030047180.91754699712-79.9687081212-13.1973148344-8.43671708904
87.5367343364-1.056747068661.493150223514.36944337969-0.6898407654390.420890868740.962360229626-0.2425266413670.09592618035620.296587287078-0.05969283225210.32446966718-0.767515012462-0.527516867336-0.7496753335980.6829837488870.05302162932170.1928465942060.5520644362170.2614144838710.518775877612-65.9442460582-38.69786764298.8619941217
91.96105224074-1.152751305463.2447219110.678393388322-1.909911755655.366841205721.236478732420.6790294579370.505436888215-1.77638228373-0.623646267784-0.06936619757450.5560075344041.50603155475-0.1582100943531.218590166760.1318047288470.1119707785160.8976491189950.3757865042850.799645238-45.6639894871-47.02612455570.616777486748
103.24871938134-2.25568812613-3.351442200683.522058139311.523377051326.42264154096-0.493766044562-0.234309037998-0.682251578009-0.191894046902-0.0406686897870.3345381743281.79841232089-0.2799178247780.4515069250660.819948507234-0.08720041224190.02350854011370.373424096774-0.01507930895250.523731713208-50.4596094061-41.0355185517-22.8583701314
111.24163104590.209954429319-0.7226526270380.7988941491770.6082012934132.19936366124-0.00198196235865-0.147613605149-0.2616651783560.1417160956280.00179544558596-0.1687017164080.406803319505-0.230015470080.02384349764670.2405221819410.249566833863-0.01612423043350.2564598548820.08198294811220.223824812519-45.3376038141-24.1359311599-27.7237293795
121.54271923053-0.151825769263-0.9589137864041.17058434961-0.3569602948172.302458559240.302773181994-0.02028186454180.3996500079150.0583410532711-0.0185607958527-0.0343277514107-0.704200673320.163322518952-0.1463692405060.4667415957650.1471253010530.1469701920910.1615251481420.04365237962020.217982187068-38.6581748831-6.0059521143-29.7424412605
130.1257601475440.447639695251-0.1187372690431.58836803706-0.4192502753050.11039488361-0.1722880127030.536395327801-0.1601722911880.1470300395150.349659636812-0.324375333588-0.08689468453290.0200254333538-0.09515423745280.3209721434380.0660600863211-0.04602589412711.178047158410.2116179618850.611149417259-60.9666247099-12.596179634-37.5358874977
142.876969862162.917782437421.102913224743.759357172850.5213126211921.95403154394-0.04787594897860.1568217339620.683405254808-0.2486933169160.1569009789180.693666742681-0.523636511246-0.6564459646240.07302025145950.5860690833930.3017428635820.1833258186280.4637257713590.2694198523330.483567888658-64.133266021-5.19201948465-20.8710820579
155.156111749642.06366560844-0.345484761912.167647055510.3219769403881.68445976738-0.2879832728280.2567899266281.29778170943-0.5161134431830.4953396120940.541931907469-0.386183480839-0.162331652067-0.04388432856540.355028741517-0.000291726670556-0.1232725835120.3195174831820.08712291805130.439046627779-13.3680981847-4.90569533587-96.2302897222
161.374697678710.150832453295-0.2268960344662.93499673231-0.7665367094522.20583175162-0.1078197060820.1447885907890.204143048776-0.154094030440.181853521659-0.209469248324-0.1305296041310.20843305707-0.02251511676540.170469130184-0.13913047865-0.09404146048980.23894528992-0.0526531168110.1886190170411.98223770065-10.8043731188-90.9613334723
170.7181268566350.0557399353612-0.2375352020210.4326187979910.06995654073370.88571099599-0.1455307013770.429259218408-0.233108360811-0.3189687630560.008046005653980.2541455545710.440016350458-0.390214229835-0.9255221703720.427684518167-0.289671492625-0.1805545530020.485545705546-0.1382320455630.228462029034-16.1273775458-29.424443824-101.833138955
180.9258861736971.177026447960.7512219573721.502290488370.9002025037770.793118448921-0.2635385671020.491837401360.0914462473302-0.0830787011160.269428789433-0.0115471960920.002018363137280.426090642973-0.03703721673110.328011589649-0.1896224479730.08158484697990.5023227986630.04451765663220.4845585189548.26891403947-8.89263670514-103.838146356
199.904786334616.964974864871.014218772746.565274632150.4681139471280.1394314385580.448675694501-1.779606330761.169364607790.966513650798-0.9849306097090.8798191360520.201717213097-0.1677333871760.3210046684580.666574315677-0.304056967508-0.09507044185740.8371899271260.01082325288550.4334260083958.656492542649.63699234466-77.4740780059
203.547749797630.277028887795-1.243079650710.945410052246-0.3743343946332.815494907110.1917882374630.113539926281.057679893280.5489536732020.222601814992-0.0583374061036-0.9196457014340.1381285479010.4235175899740.3912543791240.0397919263308-0.09640443142740.0847167577354-0.06489771961950.522221321741-12.3261064859-2.3886686506-74.4602396894
211.81155581889-0.126987272324-0.5624505455581.82393012167-0.1991112616522.49669544565-0.112730991105-0.2854258876870.1038942690090.1389312797630.124778073577-0.01841594653420.143399519348-0.04942015444760.0119731420290.0417376915490.0133694640767-0.007294502478740.0205682898917-0.03685111952680.170865213819-19.0669020319-27.4602400462-60.8727481852
221.30143203240.1914974796930.8761075554410.8641318178290.06436078575491.75126205892-0.1211055413260.08307779117830.0566357343291-0.0705286006427-0.03864021549780.189895264528-0.0310296714357-0.468483249890.09351164795560.425294751116-0.213683372577-0.2010913436430.9808901998160.102126598650.55779333662-33.8230062446-24.542543836-76.9276706449
234.19294066666-0.5579888859571.939494099221.51536554157-1.216315780192.752222617110.08622448499820.337834349192-0.375986136433-0.09413889590590.153982791883-0.135141366760.1177788026440.108187423855-0.09651521584210.328112082755-0.199128947908-0.1669970925810.589306444506-0.06205365728550.411297635466-28.5339509333-30.8306422174-91.227956474
245.11299191459-4.600399204864.153279594277.49880239396-1.994017637564.753965370590.450480462631-0.126050003501-1.790276913820.6180346287260.3936111147440.9166180921861.12334942186-0.754451147923-0.1311140411710.401859477963-0.08838954388620.007918370201260.300844748916-0.01566560322920.497160025888-21.1240625108-41.303050069-87.9863802699
253.68212315578-1.30330834647-1.02724877313.764821568561.418599977443.93022113717-0.249277440637-0.146175637274-0.423157971275-0.281327387060.20687204230.06126602480570.564702213882-0.02556316951260.01808906165720.2972930582330.01867952989920.01663738408620.141136474404-0.009968704696540.42096136232-8.84268700515-41.6719045555-78.6697766132
261.13504825399-0.665762873708-0.7995707886032.79483711240.2043502414630.8995456057130.1484942136260.398565863794-0.0205382528324-0.437442149928-0.1557571152280.196029317510.00669096822694-0.3598845051940.4838994964490.293652241601-0.106141625171-0.05364070685440.2730764896230.0205484559621-0.000690875151928-18.319167178-31.7410939971-86.0265499406
272.761191848370.04215042892241.692963606491.137883546680.585207388812.30533376558-0.3701858095470.3312183117220.697032901353-0.2551496109230.008875490999580.307354054753-0.286060899573-0.1561475387310.1500122214370.193703754919-0.169175182648-0.1309926206490.5381179878810.06902862024640.293039285799-24.1969311592-13.737602594-99.8463674661
Refinement TLS group

Refine-ID: X-RAY DIFFRACTION

IDRefine TLS-IDSelection detailsAuth asym-IDLabel asym-IDAuth seq-IDLabel seq-ID
11chain 'A' and (resid 5 through 24 )AA5 - 241 - 20
22chain 'A' and (resid 25 through 47 )AA25 - 4721 - 43
33chain 'A' and (resid 48 through 147 )AA48 - 14744 - 118
44chain 'A' and (resid 148 through 188 )AA148 - 188119 - 159
55chain 'A' and (resid 189 through 221 )AA189 - 221160 - 192
66chain 'A' and (resid 222 through 248 )AA222 - 248193 - 219
77chain 'A' and (resid 249 through 350 )AA249 - 350220 - 272
88chain 'A' and (resid 351 through 367 )AA351 - 367273 - 289
99chain 'A' and (resid 368 through 390 )AA368 - 390290 - 312
1010chain 'B' and (resid 78 through 124 )BB78 - 1241 - 47
1111chain 'B' and (resid 125 through 233 )BB125 - 23348 - 156
1212chain 'B' and (resid 234 through 399 )BB234 - 399157 - 322
1313chain 'C' and (resid 273 through 282 )CD273 - 2821 - 10
1414chain 'C' and (resid 283 through 338 )CD283 - 33811 - 66
1515chain 'D' and (resid 4 through 48 )DE4 - 481 - 45
1616chain 'D' and (resid 49 through 188 )DE49 - 18846 - 160
1717chain 'D' and (resid 189 through 332 )DE189 - 332161 - 256
1818chain 'D' and (resid 333 through 367 )DE333 - 367257 - 291
1919chain 'D' and (resid 368 through 390 )DE368 - 390292 - 314
2020chain 'E' and (resid 77 through 172 )EF77 - 1721 - 96
2121chain 'E' and (resid 173 through 399 )EF173 - 39997 - 323
2222chain 'F' and (resid 272 through 281 )FH272 - 2811 - 10
2323chain 'F' and (resid 282 through 286 )FH282 - 28611 - 15
2424chain 'F' and (resid 287 through 293 )FH287 - 29316 - 22
2525chain 'F' and (resid 294 through 311 )FH294 - 31123 - 40
2626chain 'F' and (resid 312 through 324 )FH312 - 32441 - 53
2727chain 'F' and (resid 325 through 338 )FH325 - 33854 - 67

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