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- PDB-6ypc: Crystal structure of the kinetochore subunits H/I/K/T/W penta-com... -
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Open data
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Basic information
Entry | Database: PDB / ID: 6ypc | |||||||||
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Title | Crystal structure of the kinetochore subunits H/I/K/T/W penta-complex from S. cerevisiae at 2.9 angstroms | |||||||||
![]() | (Inner kinetochore subunit ...) x 5 | |||||||||
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Function / homology | ![]() negative regulation of kinetochore assembly / attachment of spindle microtubules to kinetochore / centromeric DNA binding / establishment of mitotic sister chromatid cohesion / mitotic spindle assembly checkpoint signaling / DNA replication initiation / meiotic cell cycle / ![]() ![]() ![]() ![]() ![]() ![]() ![]() Similarity search - Function | |||||||||
Biological species | ![]() ![]() ![]() | |||||||||
Method | ![]() ![]() ![]() | |||||||||
![]() | Bellini, D. / Zhang, Z. / Barford, D. | |||||||||
Funding support | 2items
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![]() | ![]() Title: Crystal structure of the Cenp-HIKHead-TW sub-module of the inner kinetochore CCAN complex. Authors: Zhang, Z. / Bellini, D. / Barford, D. | |||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 154.3 KB | Display | ![]() |
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PDB format | ![]() | 95.8 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Unit cell |
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Components on special symmetry positions |
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Components
-Inner kinetochore subunit ... , 5 types, 5 molecules KHTWI
#1: Protein | Mass: 12748.634 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() Gene: MCM22, YJR135C, J2122 / Production host: ![]() ![]() ![]() |
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#2: Protein/peptide | Mass: 5153.744 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() Gene: MCM16, YPR046W / Production host: ![]() ![]() ![]() |
#3: Protein | Mass: 42154.633 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() Gene: CNN1, YFR046C / Production host: ![]() ![]() ![]() |
#4: Protein | Mass: 10255.458 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() Gene: WIP1, YDR374W-A / Production host: ![]() ![]() ![]() |
#5: Protein | Mass: 28856.742 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() Gene: CTF3, CHL3, YLR381W / Production host: ![]() ![]() ![]() |
-Non-polymers , 1 types, 7 molecules ![](data/chem/img/HOH.gif)
#6: Water | ChemComp-HOH / ![]() |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 4.22 Å3/Da / Density % sol: 70.85 % |
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Crystal grow![]() | Temperature: 293 K / Method: vapor diffusion, sitting drop Details: 1 M NaH2PO4 and 0.38M K2HPO4 with protein at 5.5 mg/ml |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS3 6M / Detector: PIXEL / Date: Oct 23, 2019 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Resolution: 2.9→74.07 Å / Num. obs: 24344 / % possible obs: 100 % / Redundancy: 63.3 % / Biso Wilson estimate: 78.5 Å2 / CC1/2: 0.999 / Rmerge(I) obs: 0.368 / Rpim(I) all: 0.047 / Net I/σ(I): 10 |
Reflection shell | Resolution: 2.9→3.07 Å / Num. unique obs: 3860 / CC1/2: 0.684 / Rpim(I) all: 1.826 |
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Processing
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Refinement | Method to determine structure![]() ![]() Starting model: 5Z08 and 3B0D Resolution: 2.9→68.85 Å / SU ML: 0.4099 / Cross valid method: FREE R-VALUE / σ(F): 1.34 / Phase error: 33.3898 Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 72.07 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.9→68.85 Å
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Refine LS restraints |
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LS refinement shell |
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