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- PDB-6vwa: C-terminal regulatory domain of the chloride transporter KCC-1 fr... -
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Open data
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Basic information
Entry | Database: PDB / ID: 6vwa | ||||||
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Title | C-terminal regulatory domain of the chloride transporter KCC-1 from C. elegans | ||||||
![]() | K+/Cl-Cotransporter | ||||||
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Function / homology | ![]() potassium:chloride symporter activity / chloride ion homeostasis / potassium ion homeostasis / cell volume homeostasis / potassium ion import across plasma membrane / chloride transmembrane transport / chemical synaptic transmission / membrane => GO:0016020 / ![]() ![]() Similarity search - Function | ||||||
Biological species | ![]() ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Zimanyi, C.M. / Cheung, J. | ||||||
Funding support | ![]()
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![]() | ![]() Title: Structure of the Regulatory Cytosolic Domain of a Eukaryotic Potassium-Chloride Cotransporter. Authors: Zimanyi, C.M. / Guo, M. / Mahmood, A. / Hendrickson, W.A. / Hirsh, D. / Cheung, J. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 152.4 KB | Display | ![]() |
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PDB format | ![]() | 116.9 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 6vw9SC S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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Unit cell |
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Components on special symmetry positions |
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Components
#1: Protein | Mass: 46134.020 Da / Num. of mol.: 1 / Fragment: C-terminal regulatory domain Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() ![]() ![]() | ||||||||
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#2: Chemical | ![]() #3: Chemical | ![]() #4: Chemical | ChemComp-PG4 / | ![]() #5: Water | ChemComp-HOH / | ![]() Has ligand of interest | N | |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.63 Å3/Da / Density % sol: 53.22 % |
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Crystal grow![]() | Temperature: 298 K / Method: vapor diffusion, sitting drop / pH: 7.5 / Details: 0.1 M HEPES pH 7.5, 50% w/v PEG 200 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 315r / Detector: CCD / Date: May 4, 2018 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Resolution: 2.196→50 Å / Num. obs: 24878 / % possible obs: 99.9 % / Redundancy: 5.4 % / Biso Wilson estimate: 37.95 Å2 / CC1/2: 0.995 / CC star: 0.999 / Rsym value: 0.182 / Net I/σ(I): 10.4 |
Reflection shell | Resolution: 2.196→2.28 Å / Redundancy: 5.4 % / Mean I/σ(I) obs: 0.51 / Num. unique obs: 2448 / CC1/2: 0.476 / CC star: 0.803 / % possible all: 99.71 |
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Processing
Software |
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Refinement | Method to determine structure![]() ![]() Starting model: 6VW9 Resolution: 2.196→48.955 Å / SU ML: 0.3 / Cross valid method: THROUGHOUT / σ(F): 1.35 / Phase error: 25.31
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 142.49 Å2 / Biso mean: 53.5041 Å2 / Biso min: 19.87 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: final / Resolution: 2.196→48.955 Å
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Refine LS restraints |
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LS refinement shell | Refine-ID: X-RAY DIFFRACTION / Rfactor Rfree error: 0 / % reflection obs: 100 %
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Refinement TLS params. | Method: refined / Refine-ID: X-RAY DIFFRACTION
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Refinement TLS group |
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